##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931045_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2518804 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37810484658592 31.0 31.0 34.0 30.0 34.0 2 31.5329684246968 31.0 31.0 34.0 30.0 34.0 3 31.59608171179655 31.0 31.0 34.0 30.0 34.0 4 35.36935545600213 37.0 35.0 37.0 33.0 37.0 5 35.25059671177273 37.0 35.0 37.0 33.0 37.0 6 35.27998883597136 37.0 35.0 37.0 32.0 37.0 7 35.16364869993854 37.0 35.0 37.0 32.0 37.0 8 35.16663305282984 37.0 35.0 37.0 32.0 37.0 9 36.67855101071778 39.0 35.0 39.0 32.0 39.0 10-11 36.62100743051067 39.0 35.0 39.0 32.0 39.0 12-13 36.50751070746274 38.0 35.0 39.0 32.0 39.0 14-15 37.577867908737645 39.0 36.0 41.0 32.0 41.0 16-17 37.535562910016026 39.0 36.0 41.0 32.0 41.0 18-19 37.5523589767207 39.0 36.0 41.0 32.0 41.0 20-21 37.53795610932808 39.0 36.0 41.0 32.0 41.0 22-23 37.437977508373024 39.0 36.0 41.0 32.0 41.0 24-25 37.31691548846199 39.0 36.0 41.0 31.5 41.0 26-27 37.07085763719606 39.0 36.0 40.0 31.0 41.0 28-29 36.88779793902185 39.0 36.0 40.0 30.5 41.0 30-31 36.65799145149841 38.5 35.5 40.0 30.0 41.0 32-33 36.49491544399643 38.0 35.0 40.0 30.0 41.0 34-35 36.578484471201406 38.0 35.0 40.0 30.0 41.0 36-37 36.64844148254489 39.0 35.0 40.0 30.0 41.0 38-39 36.62740133809538 39.0 35.0 40.0 30.0 41.0 40-41 36.51502240746005 39.0 35.0 40.0 30.0 41.0 42-43 36.37383913158784 39.0 35.0 40.0 30.0 41.0 44-45 36.20956116474326 39.0 35.0 40.0 30.0 41.0 46-47 35.972639594029545 38.0 35.0 40.0 29.0 41.0 48-49 35.757738990409734 38.0 35.0 40.0 28.0 41.0 50-51 35.494932912604554 38.0 34.5 40.0 27.5 41.0 52-53 35.28984490258075 38.0 34.0 40.0 27.0 41.0 54-55 35.135300722088736 38.0 34.0 40.0 26.5 41.0 56-57 34.96406747011677 38.0 34.0 40.0 26.0 41.0 58-59 34.76510399380024 38.0 34.0 40.0 26.0 41.0 60-61 34.46264040393774 37.5 33.0 40.0 26.0 41.0 62-63 34.21092887735608 37.0 33.0 40.0 25.5 41.0 64-65 33.90340375829163 37.0 32.5 39.5 25.0 41.0 66-67 33.58071092470871 36.0 32.0 39.0 24.0 41.0 68-69 33.23164426450013 36.0 32.0 39.0 23.0 40.0 70-71 32.85151286086571 35.5 31.5 39.0 22.5 40.0 72-73 32.4221271285896 35.0 31.0 38.0 22.0 40.0 74-75 31.900659003241223 35.0 31.0 37.0 21.0 39.5 76-77 30.522254411220565 33.5 29.5 36.0 20.0 38.5 78-79 31.24787180741336 34.0 30.5 36.0 20.5 39.0 80-81 31.209611784005425 34.0 31.0 36.0 20.5 38.5 82-83 30.93893530421581 34.0 30.5 36.0 20.0 37.0 84-85 30.595412743508426 34.0 30.0 35.0 19.5 37.0 86-87 30.251928097620933 34.0 30.0 35.0 18.0 36.5 88-89 29.90739354868422 34.0 30.0 35.0 16.5 36.0 90-91 29.576854729466845 34.0 29.0 35.0 10.0 36.0 92-93 29.258666216188317 34.0 29.0 35.0 6.5 35.0 94-95 28.884362181416257 34.0 29.0 35.0 2.0 35.0 96-97 28.456354484112303 34.0 29.0 35.0 2.0 35.0 98-99 27.96596539468732 34.0 29.0 35.0 2.0 35.0 100-101 26.836179393077032 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28305467694759656 1101 2 0.43130647319199866 1101 3 0.3079042595465715 1101 4 0.1691298358959159 1101 5 0.23997421066385982 1101 6 0.34373580596984254 1101 7 0.4148569109938336 1101 8 0.2804839827940597 1101 9 0.3969138130973562 1101 10-11 0.4111572290154655 1101 12-13 0.49153478580765153 1101 14-15 0.4608903972786962 1101 16-17 0.5090944083018556 1101 18-19 0.4624263718906718 1101 20-21 0.47014979539266477 1101 22-23 0.6450366307583195 1101 24-25 0.6058339576552143 1101 26-27 0.44448480877331775 1101 28-29 0.6334019294806339 1101 30-31 0.49572620869175665 1101 32-33 0.48261052811614746 1101 34-35 0.39319493698261 1101 36-37 0.565870854934154 1101 38-39 0.5238503766516445 1101 40-41 0.6151345287766077 1101 42-43 0.6079916104101031 1101 44-45 0.6800672105877013 1101 46-47 0.515978670072613 1101 48-49 0.5049631813064437 1101 50-51 0.5578808470906864 1101 52-53 0.45405824182883947 1101 54-55 0.3646246118474963 1101 56-57 0.30847098633781656 1101 58-59 0.2866754741267741 1101 60-61 0.42839623931327964 1101 62-63 0.28934618889214647 1101 64-65 0.2553679013471921 1101 66-67 0.2712184705515668 1101 68-69 0.3184374528345515 1101 70-71 0.4087149790178586 1101 72-73 0.37177940076868765 1101 74-75 0.3222403166696921 1101 76-77 0.29564098768855374 1101 78-79 0.2657248112611583 1101 80-81 0.3252883559493931 1101 82-83 0.38374754035248415 1101 84-85 0.32431330953764004 1101 86-87 0.470213119533728 1101 88-89 0.4265102671389407 1101 90-91 0.34767836007190667 1101 92-93 0.25366608944561264 1101 94-95 0.5244956919315271 1101 96-97 0.5956671454030911 1101 98-99 0.6210441419588797 1101 100-101 0.7322320563767377 1102 1 0.24675862524704684 1102 2 0.3834579897729675 1102 3 0.3165386290923067 1102 4 0.18766750276699184 1102 5 0.27022721001917205 1102 6 0.2954055388188763 1102 7 0.31904390094618407 1102 8 0.3494230836950436 1102 9 0.46954333964735184 1102 10-11 0.38661456190852306 1102 12-13 0.40466056218075863 1102 14-15 0.44688989411030633 1102 16-17 0.3911106983784336 1102 18-19 0.45384499226585007 1102 20-21 0.5597508772046993 1102 22-23 0.5096994293179975 1102 24-25 0.4615427175023825 1102 26-27 0.35079218176260696 1102 28-29 0.4460569289302967 1102 30-31 0.45417299077266904 1102 32-33 0.6143834104848125 1102 34-35 0.5833856692498571 1102 36-37 0.5875221906889934 1102 38-39 0.4495124361827507 1102 40-41 0.6090667897323172 1102 42-43 0.7048997979032166 1102 44-45 0.6921026839590851 1102 46-47 0.6461277337077007 1102 48-49 0.5606250835974294 1102 50-51 0.48860619577065734 1102 52-53 0.4435530293188137 1102 54-55 0.5139829149121056 1102 56-57 0.3463337786078924 1102 58-59 0.5489054535283202 1102 60-61 0.33099557575997096 1102 62-63 0.195241435523279 1102 64-65 0.248199166972924 1102 66-67 0.21835135417815366 1102 68-69 0.416767877225638 1102 70-71 0.2144361608346088 1102 72-73 0.17260805620840358 1102 74-75 0.30886201266164903 1102 76-77 0.3480805681719126 1102 78-79 0.4041142006977694 1102 80-81 0.3719048630173347 1102 82-83 0.2127704502381711 1102 84-85 0.3256534670922413 1102 86-87 0.3388223306600402 1102 88-89 0.40346908595114783 1102 90-91 0.3142750548845541 1102 92-93 0.35795748683856843 1102 94-95 0.4346611085392311 1102 96-97 0.40777384854946774 1102 98-99 0.4369162742921624 1102 100-101 0.7305096480870397 1103 1 0.2960436012444738 1103 2 0.3389882284205541 1103 3 0.30563939395896966 1103 4 0.12009876812749098 1103 5 0.21532371797026428 1103 6 0.2582347032843515 1103 7 0.26707247434352865 1103 8 0.2691759375894307 1103 9 0.47206410742342086 1103 10-11 0.3726181910056141 1103 12-13 0.5103788401205875 1103 14-15 0.504647816490511 1103 16-17 0.5533641073562379 1103 18-19 0.502152562072375 1103 20-21 0.4885322968401056 1103 22-23 0.3573674664664779 1103 24-25 0.44970313278134455 1103 26-27 0.2928637530697742 1103 28-29 0.382963391632515 1103 30-31 0.4764649876182645 1103 32-33 0.4948783152018805 1103 34-35 0.4701465086891048 1103 36-37 0.48625024575326137 1103 38-39 0.4088761957435878 1103 40-41 0.403246223317538 1103 42-43 0.43623437156087874 1103 44-45 0.4421527563113372 1103 46-47 0.44563413056553713 1103 48-49 0.2926530904268674 1103 50-51 0.20353702089258974 1103 52-53 0.3458568169017795 1103 54-55 0.4025901531998457 1103 56-57 0.3810630127459049 1103 58-59 0.4404936811661102 1103 60-61 0.26487610649628124 1103 62-63 0.29861435204638553 1103 64-65 0.28023766296484354 1103 66-67 0.28413999372612153 1103 68-69 0.18626079895505399 1103 70-71 0.27968449367028825 1103 72-73 0.21273561054916001 1103 74-75 0.340280958998477 1103 76-77 0.4838577997986526 1103 78-79 0.37426449431775666 1103 80-81 0.311251778667625 1103 82-83 0.4415658043813906 1103 84-85 0.4076336219030914 1103 86-87 0.3591577541695443 1103 88-89 0.47538148230367483 1103 90-91 0.43977788389589634 1103 92-93 0.35758186763344924 1103 94-95 0.5208577535385039 1103 96-97 0.5945161596871174 1103 98-99 0.47285114960894603 1103 100-101 0.6312302612053848 1104 1 0.36733218288507175 1104 2 0.4051718883842348 1104 3 0.35394181301462524 1104 4 0.17261274381927194 1104 5 0.26407012146398046 1104 6 0.24397653727584157 1104 7 0.2946699594309976 1104 8 0.27127924266535075 1104 9 0.531454445740458 1104 10-11 0.45831378286370494 1104 12-13 0.4116557825337708 1104 14-15 0.5294581075761258 1104 16-17 0.5059494323365143 1104 18-19 0.5325699179366907 1104 20-21 0.5137033065449614 1104 22-23 0.5361444257426697 1104 24-25 0.34326790661832973 1104 26-27 0.5837006157696081 1104 28-29 0.5721940709272104 1104 30-31 0.502330004057832 1104 32-33 0.6014226638061047 1104 34-35 0.315428874247786 1104 36-37 0.4453773711488864 1104 38-39 0.5800893809721899 1104 40-41 0.5798797060696756 1104 42-43 0.6971221455754986 1104 44-45 0.7845753044852231 1104 46-47 0.6903424791068176 1104 48-49 0.748386552868439 1104 50-51 0.5811932267442685 1104 52-53 0.5114992865165036 1104 54-55 0.5408987253821635 1104 56-57 0.7907513767411913 1104 58-59 0.7110118853524838 1104 60-61 0.7570378775175257 1104 62-63 0.6895562900639405 1104 64-65 0.5191216012057325 1104 66-67 0.4509515574072438 1104 68-69 0.2963674406594592 1104 70-71 0.5401578122590962 1104 72-73 0.5149625027855009 1104 74-75 0.604570115480513 1104 76-77 0.643714906360163 1104 78-79 0.747981574902731 1104 80-81 0.5733778975286477 1104 82-83 0.5508604731955309 1104 84-85 0.609436915212374 1104 86-87 0.551833884609831 1104 88-89 0.6064780054498478 1104 90-91 0.5956814754182211 1104 92-93 0.6083184066383502 1104 94-95 0.8013590122688612 1104 96-97 0.8019140766176598 1104 98-99 0.8525193754174509 1104 100-101 1.0774299747589744 1105 1 0.34084422316385243 1105 2 0.37037036652843724 1105 3 0.3525547426887101 1105 4 0.14790386518937737 1105 5 0.24131621315731877 1105 6 0.2158097141098807 1105 7 0.31711115963172887 1105 8 0.31362686282304253 1105 9 0.30682806511529037 1105 10-11 0.35759940533511525 1105 12-13 0.37477852082846397 1105 14-15 0.5328933215879985 1105 16-17 0.5347794348855217 1105 18-19 0.4957759530724175 1105 20-21 0.4176656799021714 1105 22-23 0.445916517742468 1105 24-25 0.42641151632724217 1105 26-27 0.4603960187571161 1105 28-29 0.5498069011098607 1105 30-31 0.37369804029304987 1105 32-33 0.43962032931590755 1105 34-35 0.513492277159763 1105 36-37 0.6301134973592184 1105 38-39 0.6260867390026377 1105 40-41 0.4451036017329386 1105 42-43 0.4293915741824037 1105 44-45 0.5593566956659188 1105 46-47 0.43419009599654146 1105 48-49 0.28926700120442206 1105 50-51 0.3774453046264554 1105 52-53 0.39470702099493593 1105 54-55 0.4854943435471242 1105 56-57 0.409951770640113 1105 58-59 0.47812648132649116 1105 60-61 0.3563671455002364 1105 62-63 0.20071516190372307 1105 64-65 0.13383892571410883 1105 66-67 0.172951073748159 1105 68-69 0.1293168968334868 1105 70-71 0.10059827607798866 1105 72-73 0.3717378329772316 1105 74-75 0.3906954906612441 1105 76-77 0.4194344218586785 1105 78-79 0.3684662071075948 1105 80-81 0.3070017894155903 1105 82-83 0.29614250961811095 1105 84-85 0.2564297685322252 1105 86-87 0.35101057485669784 1105 88-89 0.41267319892437015 1105 90-91 0.3660889472973672 1105 92-93 0.30172103539079487 1105 94-95 0.3922923653552388 1105 96-97 0.5157082368337242 1105 98-99 0.5865299040800593 1105 100-101 0.7164529834234443 1106 1 0.2741904760361429 1106 2 0.32140744347564976 1106 3 0.3080450853333403 1106 4 0.22624823337068278 1106 5 0.23359402015786657 1106 6 0.27898498185312803 1106 7 0.4119872846786663 1106 8 0.3390533673281979 1106 9 0.42295981317860054 1106 10-11 0.3915389631887365 1106 12-13 0.3579479108913475 1106 14-15 0.3932850675643067 1106 16-17 0.39083994091972585 1106 18-19 0.39859354834869265 1106 20-21 0.3496898096257013 1106 22-23 0.38199866045510333 1106 24-25 0.4778676264528272 1106 26-27 0.44853084195464277 1106 28-29 0.39635564503962684 1106 30-31 0.5258368983619803 1106 32-33 0.5701042654309205 1106 34-35 0.49230153578408675 1106 36-37 0.449568690339504 1106 38-39 0.4628509197938371 1106 40-41 0.3604485356764684 1106 42-43 0.5336905862635959 1106 44-45 0.46617728941102143 1106 46-47 0.5417827386740299 1106 48-49 0.5884855110704876 1106 50-51 0.5323500798158349 1106 52-53 0.3917706653413262 1106 54-55 0.4797056092431191 1106 56-57 0.5827410920022871 1106 58-59 0.637890848812134 1106 60-61 0.6810492857993324 1106 62-63 0.5453139072887154 1106 64-65 0.4834138231918885 1106 66-67 0.3873856771005748 1106 68-69 0.3687790606858954 1106 70-71 0.6081804127567665 1106 72-73 0.6260164364460508 1106 74-75 0.7654150053408983 1106 76-77 0.7195774596114148 1106 78-79 0.6280887436106184 1106 80-81 0.6461247798771375 1106 82-83 0.6790902089108712 1106 84-85 0.8063079202431105 1106 86-87 0.7644777035033563 1106 88-89 0.6282952640771811 1106 90-91 0.664094795163404 1106 92-93 0.7643151368404091 1106 94-95 0.9601840456690525 1106 96-97 0.8367662334959398 1106 98-99 0.9365362573698199 1106 100-101 1.1812935939355462 1107 1 0.06213988886360511 1107 2 0.10611594184268469 1107 3 0.034621417763329276 1107 4 -0.17100352119814488 1107 5 0.02857143659869621 1107 6 0.14671272836135785 1107 7 0.09736634103333586 1107 8 0.037505872366068616 1107 9 0.07655859019201472 1107 10-11 0.08406554230068508 1107 12-13 0.09224226152715431 1107 14-15 0.18388335816259627 1107 16-17 0.06335470709303337 1107 18-19 0.16271209466236058 1107 20-21 0.19321492949227803 1107 22-23 0.08394779533106345 1107 24-25 0.0691462033411625 1107 26-27 0.20620612511773118 1107 28-29 0.17425954330938254 1107 30-31 0.18883530014085892 1107 32-33 0.08517861745649213 1107 34-35 0.08170810933700068 1107 36-37 0.013208465198793817 1107 38-39 0.0638588414115091 1107 40-41 0.035885961845607994 1107 42-43 -0.01770368793555832 1107 44-45 -0.00982542061607461 1107 46-47 -0.07503784363434818 1107 48-49 -0.012773609213027726 1107 50-51 -0.003441734512570349 1107 52-53 0.023182658948442736 1107 54-55 0.15475633539140432 1107 56-57 0.17181308144013485 1107 58-59 0.14451547531481168 1107 60-61 -0.10108503426181414 1107 62-63 -0.19572806338739923 1107 64-65 -0.2143062796501667 1107 66-67 -0.20224777436214936 1107 68-69 -0.11086828835170337 1107 70-71 -0.09183834896931131 1107 72-73 -0.15109564749529625 1107 74-75 -0.02808787840589133 1107 76-77 0.028647016973653905 1107 78-79 0.08477868871519689 1107 80-81 0.05529266258160703 1107 82-83 0.13732382714448832 1107 84-85 0.13897590717938613 1107 86-87 0.20805980342692365 1107 88-89 0.29386124281389314 1107 90-91 0.3804842052113493 1107 92-93 0.2458768768167836 1107 94-95 0.24713387542376353 1107 96-97 0.32585663722048963 1107 98-99 0.3261210673204893 1107 100-101 0.5101501709628558 1108 1 0.021053802777519337 1108 2 0.030430256157000457 1108 3 -0.022700904558991652 1108 4 -0.06557309076771389 1108 5 -0.014726861699607241 1108 6 -0.04813712148848737 1108 7 0.029313287980279767 1108 8 0.046820186680385234 1108 9 0.0915385701719913 1108 10-11 0.02817214890728792 1108 12-13 0.05702454380944033 1108 14-15 -0.024337362558121356 1108 16-17 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-1.2223812907264637 2316 72-73 -1.7595571297080141 2316 74-75 -3.0129586326744757 2316 76-77 -1.8373567854467332 2316 78-79 -1.664820046248792 2316 80-81 -1.5085691706237618 2316 82-83 -0.8713464225721559 2316 84-85 -0.96768838297454 2316 86-87 -1.0294539986278473 2316 88-89 -1.0695115771926886 2316 90-91 -0.8055660306118355 2316 92-93 -0.6552091895062055 2316 94-95 -1.1858537667099824 2316 96-97 -0.9378448727791451 2316 98-99 -1.222928876008826 2316 100-101 -1.5181952277428614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 27.0 5 52.0 6 103.0 7 324.0 8 742.0 9 1505.0 10 2729.0 11 4397.0 12 6357.0 13 8284.0 14 10189.0 15 12950.0 16 15486.0 17 18091.0 18 15739.0 19 12658.0 20 12034.0 21 12499.0 22 13865.0 23 16191.0 24 19875.0 25 25448.0 26 32915.0 27 42323.0 28 53794.0 29 68178.0 30 83464.0 31 101801.0 32 123646.0 33 150897.0 34 187891.0 35 236427.0 36 312533.0 37 429549.0 38 410858.0 39 74948.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.368308170941745 21.552257389310224 14.93481176445365 23.144622675294375 2 14.995626575018631 21.78700169656332 40.956221678450234 22.26115004996782 3 18.407900744416875 26.608794044665014 31.01256575682382 23.970739454094293 4 12.475229176103406 18.39103680935483 39.48633374308177 29.647400271459993 5 12.16936111419905 38.56159380012387 36.37690768473376 12.892137400943321 6 29.171235666706103 39.85844768074779 17.900051850375537 13.07026480217057 7 25.48153806330306 33.52039301192153 23.343301026995352 17.654767897780058 8 23.970187189607046 37.790075603972234 20.551115360076672 17.68862184634405 9 24.68004656178091 16.868243817303767 21.48265605422256 36.969053566692764 10-11 23.504508488949515 27.678811054770442 29.336562114400326 19.480118341879717 12-13 24.264452494120224 24.94521209272337 29.77373388322394 21.016601529932462 14-15 21.342331519244848 26.83202027628986 27.253807759555727 24.57184044490957 16-17 20.653750748371053 30.020696330480657 28.092757515074613 21.232795406073677 18-19 20.467888728142405 28.76833608331573 30.41248941958167 20.35128576896019 20-21 21.201054151097107 27.853417733178127 30.527385219334256 20.41814289639051 22-23 20.871352475010887 27.75964081265779 30.345637222848843 21.02336948948248 24-25 20.756696818054863 28.035471007005548 30.486350685517788 20.7214814894218 26-27 20.77871856985664 28.16314344411423 30.285414476004973 20.772723510024157 28-29 20.756784826745907 28.225220695327003 30.286578869584947 20.731415608342147 30-31 20.97577360642056 27.949348422850584 30.31443729160529 20.760440679123565 32-33 20.9505380460227 28.111817634185886 30.142887265469792 20.79475705432162 34-35 20.98425989419771 28.075248831533102 30.266697531954907 20.673793742314288 36-37 20.95594825503573 28.08913375204191 30.276406024909313 20.67851196801304 38-39 20.869931506958086 28.07867330264142 30.354860020422457 20.696535169978038 40-41 20.928178208183226 28.13052602826309 30.230307587616455 20.710988175937224 42-43 20.983233258322574 28.156849587705512 29.97678206809336 20.883135085878553 44-45 20.877298407837472 28.40702154772547 29.87191192314358 20.84376812129348 46-47 21.005318347435058 28.47369642517874 29.58864550987862 20.93233971750759 48-49 21.164479139762232 28.46228171301889 29.395394470529684 20.977844676689195 50-51 21.124605341010227 28.607926492743246 29.214381249285264 21.053086916961263 52-53 21.089548121851138 28.680681306906074 29.208446145212143 21.02132442603065 54-55 21.127810875744604 28.641394587084363 29.217343550807062 21.013450986363974 56-57 21.108204561864007 28.760107383285792 29.14428274131114 20.987405313539064 58-59 21.060052907741827 28.740490782442123 29.32363146287285 20.875824846943193 60-61 21.02525565904125 28.826915837922083 29.314563075150858 20.833265427885806 62-63 21.075576131859194 28.776588060995106 29.208575033934586 20.939260773211117 64-65 20.89846940714574 28.564168554844237 29.274496081936196 21.262865956073828 66-67 20.97453436247151 28.706284479783868 29.15533509543992 21.163846062304703 68-69 20.973224544572098 28.762371962205446 29.113715988421724 21.150687504800732 70-71 21.008947762900103 28.481725540788418 29.180518942790705 21.328807753520778 72-73 21.050352451653463 28.719014097668495 29.132007031934382 21.09862641874366 74-75 20.980029238322587 28.77667638623251 29.000582059199644 21.242712316245264 76-77 20.930165937933367 28.83010703317983 28.963552857815543 21.27617417107126 78-79 20.848067480125902 28.827207243512692 29.096913348030256 21.22781192833115 80-81 20.94359162482633 28.861484321015375 28.910614830108685 21.284309224049615 82-83 21.025951205413364 28.74524972963359 29.075882839633415 21.152916225319636 84-85 21.138405370167746 28.738560046752347 28.832850829203068 21.29018375387684 86-87 20.880644146984046 28.920193869788996 28.874358624172423 21.32480335905454 88-89 20.91405376723811 28.773724328812733 28.918316905877656 21.393904998071502 90-91 20.959178418803205 28.752860405085173 29.0021402291818 21.28582094692982 92-93 20.899499472868094 28.68649847419635 28.94839666527025 21.465605387665313 94-95 20.72755454217368 28.77602641639328 28.95363513250083 21.542783908932208 96-97 20.74617968412383 28.697812274756647 28.984098997797965 21.57190904332156 98-99 20.686881551720578 28.75070866967021 28.895995877408488 21.66641390120073 100-101 20.694841678828524 28.68538004545014 28.86219015056352 21.757588125157813 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 116.0 1 96.5 2 115.0 3 165.5 4 146.5 5 151.5 6 281.0 7 623.0 8 1142.0 9 1478.0 10 1464.0 11 1371.0 12 1457.5 13 1634.5 14 1958.0 15 2344.0 16 2623.0 17 2934.5 18 3321.0 19 3731.0 20 4238.0 21 5107.5 22 6373.5 23 8099.0 24 10322.0 25 13184.5 26 17045.5 27 21604.0 28 26546.5 29 33180.5 30 40665.5 31 47247.0 32 54502.5 33 61353.5 34 66913.0 35 72752.0 36 80564.0 37 88906.5 38 93855.0 39 95710.0 40 97757.0 41 99482.0 42 101426.0 43 103806.5 44 104394.5 45 105236.0 46 105875.5 47 105166.5 48 102938.0 49 99293.5 50 93392.0 51 86406.5 52 80867.0 53 73573.5 54 64738.5 55 55384.0 56 44515.0 57 34158.0 58 26076.0 59 19590.0 60 13937.5 61 9605.0 62 6503.0 63 4086.5 64 2336.0 65 1372.0 66 745.0 67 396.0 68 212.5 69 113.5 70 55.5 71 31.0 72 21.5 73 16.0 74 13.5 75 9.0 76 5.5 77 2.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003890735444282286 2 0.007265352921465902 3 0.002143874632563709 4 0.1688499780054343 5 0.0017468608117185774 6 7.146248775212363E-4 7 0.0 8 8.337290237747757E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.779096745915919E-4 24-25 5.756700402254404E-4 26-27 0.0025210377623665833 28-29 1.3895483729579595E-4 30-31 0.005796401784338916 32-33 0.02084322559436939 34-35 0.01322056023414287 36-37 0.001091788007324111 38-39 3.1761105667610503E-4 40-41 0.0016674580475495515 42-43 0.010977432146367878 44-45 0.007364606376677184 46-47 0.010262807268846643 48-49 0.009706987919663458 50-51 0.18923663770583182 52-53 0.11179909194998895 54-55 0.017409056044059006 56-57 0.006451474588733383 58-59 0.018183232994707012 60-61 0.12112891673984955 62-63 0.23348382803902168 64-65 0.23898246945772678 66-67 0.24936438087282695 68-69 0.24483842331519245 70-71 0.23143920686166927 72-73 0.15844821589929187 74-75 0.2791801188182963 76-77 0.21536014711744145 78-79 0.0054192386545360415 80-81 9.925345521128282E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.925345521128282E-5 90-91 0.005895655239550199 92-93 0.018222934376791525 94-95 0.002580589835493353 96-97 1.9850691042256564E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2518804.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.33377227037825 #Duplication Level Percentage of deduplicated Percentage of total 1 72.91678010873521 41.076772853347364 2 15.738180344528304 17.731821349576013 3 5.047665392418324 8.5306209814069 4 2.260235756093813 5.093104254446202 5 1.1767745188822867 3.314607388014933 6 0.7194634873892444 2.431805535326271 7 0.4456859044476757 1.757501777169105 8 0.2941958677852296 1.3258530414959548 9 0.2292932430517804 1.162525800349394 >10 1.0716472460533781 10.72437188808124 >50 0.0678551398928549 2.6425994569988007 >100 0.030880710413158933 3.087512219546853 >500 7.771096523617704E-4 0.27106243305332806 >1k 4.238779921876431E-4 0.37238748877284056 >5k 1.412926640625477E-4 0.4774535324150655 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6331 0.2513494499770526 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5666 0.22494803089085136 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3092 0.12275667340531457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.970138208451313E-5 0.0 4 0.0 0.0 0.0 3.970138208451313E-5 0.0 5 0.0 0.0 0.0 3.970138208451313E-5 0.0 6 7.940276416902626E-5 0.0 0.0 3.970138208451313E-5 0.0 7 7.940276416902626E-5 0.0 0.0 3.970138208451313E-5 0.0 8 7.940276416902626E-5 0.0 0.0 3.970138208451313E-5 0.0 9 7.940276416902626E-5 0.0 0.0 3.970138208451313E-5 0.0 10-11 7.940276416902626E-5 0.0 0.0 1.1910414625353938E-4 0.0 12-13 7.940276416902626E-5 0.0 0.0 1.1910414625353938E-4 0.0 14-15 7.940276416902626E-5 0.0 0.0 1.1910414625353938E-4 0.0 16-17 1.1910414625353938E-4 0.0 0.0 1.1910414625353938E-4 0.0 18-19 1.1910414625353938E-4 0.0 0.0 1.1910414625353938E-4 0.0 20-21 1.1910414625353938E-4 0.0 0.0 1.1910414625353938E-4 0.0 22-23 1.1910414625353938E-4 0.0 0.0 2.183576014648222E-4 0.0 24-25 1.1910414625353938E-4 0.0 0.0 3.7716312980287474E-4 0.0 26-27 1.1910414625353938E-4 0.0 0.0 5.359686581409272E-4 0.0 28-29 1.1910414625353938E-4 0.0 0.0 8.932810969015454E-4 0.0 30-31 1.5880552833805252E-4 0.0 0.0 0.0029180515832117147 0.0 32-33 1.5880552833805252E-4 0.0 0.0 0.0069080404827052844 0.0 34-35 1.5880552833805252E-4 0.0 0.0 0.013677126128114774 0.0 36-37 1.5880552833805252E-4 0.0 0.0 0.024178141689468493 0.0 38-39 1.5880552833805252E-4 0.0 0.0 0.04099167700225981 0.0 40-41 1.5880552833805252E-4 0.0 0.0 0.06108057633702345 0.0 42-43 1.5880552833805252E-4 0.0 0.0 0.08236051713432248 0.0 44-45 1.5880552833805252E-4 0.0 0.0 0.10929790487866464 0.0 46-47 1.5880552833805252E-4 0.0 0.0 0.1400664759941623 0.0 48-49 1.5880552833805252E-4 0.0 0.0 0.1753014525941677 0.0 50-51 1.5880552833805252E-4 0.0 0.0 0.2161938761412162 0.0 52-53 1.5880552833805252E-4 0.0 0.0 0.2557761540794758 0.0 54-55 1.5880552833805252E-4 0.0 0.0 0.29972558404703187 0.0 56-57 1.5880552833805252E-4 0.0 0.0 0.3546127447788713 0.0 58-59 1.5880552833805252E-4 0.0 0.0 0.4205368897302053 0.0 60-61 1.7865621938030908E-4 0.0 0.0 0.4919398254092021 0.0 62-63 1.9850691042256564E-4 0.0 0.0 0.5671143923862277 0.0 64-65 1.9850691042256564E-4 0.0 0.0 0.6459613372060709 0.0 66-67 1.9850691042256564E-4 0.0 0.0 0.7412446542089023 0.0 68-69 2.779096745915919E-4 0.0 0.0 0.8496492779906654 0.0 70-71 2.977603656338485E-4 0.0 0.0 0.9697261081052754 0.0 72-73 3.5731243876061815E-4 0.0 0.0 1.098437988823267 0.0 74-75 3.970138208451313E-4 0.0 0.0 1.2302267266528082 0.0 76-77 3.970138208451313E-4 0.0 0.0 1.383255703897564 1.9850691042256564E-5 78-79 3.970138208451313E-4 0.0 0.0 1.5548252265757876 3.970138208451313E-5 80-81 3.970138208451313E-4 0.0 0.0 1.7503942347240993 3.970138208451313E-5 82-83 3.970138208451313E-4 0.0 0.0 1.9551938142070602 3.970138208451313E-5 84-85 3.970138208451313E-4 0.0 0.0 2.167933670106924 3.970138208451313E-5 86-87 4.168645118873878E-4 0.0 0.0 2.3967922871330996 3.970138208451313E-5 88-89 4.367152029296444E-4 0.0 0.0 2.6605682697026047 3.970138208451313E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3565 0.0 54.91849 1 GTATCAA 6590 0.0 41.246918 1 ATCAACG 8010 0.0 33.517445 3 TCAACGC 8060 0.0 33.309525 4 CAACGCA 8055 0.0 33.220352 5 TATCAAC 8390 0.0 31.886103 2 AACGCAG 8460 0.0 31.796997 6 ACGCAGA 9650 0.0 27.728424 7 CGCAGAG 9765 0.0 27.353289 8 GTGGTAT 1855 0.0 25.105165 1 GCAGAGT 11285 0.0 23.248146 9 GTACATG 6250 0.0 22.049507 1 TGGTATC 1925 0.0 21.722355 2 ACATGGG 5995 0.0 21.48004 3 TACATGG 6265 0.0 21.161095 2 GAGTACT 8065 0.0 20.941626 12-13 AGAGTAC 10210 0.0 20.677547 10-11 CAGAGTA 10760 0.0 20.458344 10-11 ACCGGGC 175 1.00345685E-4 18.977255 8 AGTACTT 8740 0.0 18.835747 12-13 >>END_MODULE