FastQCFastQC Report
Sat 18 Jun 2016
SRR1931044_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931044_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967293
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN795644.04433909946307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.060.0764241
GTATCAA34300.047.667431
ATCAACG41150.038.5964623
TCAACGC42400.038.166194
CAACGCA42200.037.9811255
TATCAAC44200.036.1746642
AACGCAG46200.034.7513966
ACGCAGA52250.030.427357
CGCAGAG53400.030.0117598
GCAGAGT59150.026.7445019
GTACATG34350.025.207691
GAGTACT39900.023.74475312-13
TACATGG35200.023.2905852
ACATGGG35000.023.0470813
CAGAGTA56150.022.49629810-11
GTACTAG5000.022.4111181
GCGTGCG4550.022.2746669
AGAGTAC53500.022.02225110-11
GGCCGCG1453.225606E-521.991517
AACGTGC1453.7706035E-521.5510448
Produced by FastQC (version 0.11.5)