##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931044_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1967293 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.89939475207811 30.0 10.0 31.0 2.0 33.0 2 21.497886181671973 30.0 2.0 31.0 2.0 34.0 3 22.254911698460777 30.0 10.0 31.0 2.0 34.0 4 23.87157378184134 33.0 2.0 35.0 2.0 37.0 5 23.23590080379486 33.0 2.0 35.0 2.0 37.0 6 24.29728718599619 33.0 2.0 36.0 2.0 37.0 7 24.564156940526907 33.0 10.0 36.0 2.0 37.0 8 25.062568209209303 33.0 10.0 36.0 2.0 37.0 9 26.58179183273666 34.0 17.0 38.0 2.0 39.0 10-11 27.041114872060234 35.0 17.0 38.0 2.0 39.0 12-13 27.007891554537125 35.0 14.0 38.0 2.0 39.0 14-15 27.073452454718236 35.5 10.0 39.0 2.0 40.0 16-17 25.880334805237453 34.0 2.0 38.5 2.0 40.0 18-19 24.754574432989898 33.0 2.0 38.5 2.0 40.0 20-21 24.420990925093516 32.5 2.0 38.5 2.0 40.0 22-23 24.066408765750705 32.0 2.0 38.0 2.0 40.0 24-25 23.93008819733512 32.0 2.0 38.0 2.0 40.0 26-27 23.64819704029852 32.0 2.0 38.0 2.0 40.0 28-29 23.492934199430387 31.5 2.0 38.0 2.0 40.0 30-31 23.560504205525056 31.0 2.0 38.0 2.0 40.0 32-33 23.47233406513417 31.0 2.0 38.0 2.0 40.0 34-35 23.173164597240984 30.0 2.0 38.0 2.0 40.0 36-37 22.6737717767511 30.0 2.0 38.0 2.0 40.0 38-39 22.597226747617157 30.0 2.0 37.5 2.0 40.0 40-41 22.52642921008716 30.0 2.0 37.0 2.0 40.0 42-43 22.407690923517748 30.0 2.0 37.0 2.0 39.0 44-45 22.013584910839413 30.0 2.0 37.0 2.0 39.0 46-47 21.682967153342183 29.0 2.0 37.0 2.0 39.0 48-49 21.48505026958364 28.5 2.0 37.0 2.0 39.0 50-51 18.805425018032395 17.5 2.0 34.5 2.0 38.5 52-53 21.055667610264457 27.5 2.0 36.0 2.0 38.5 54-55 22.000938599385044 30.0 2.0 37.5 2.0 39.5 56-57 22.01987578871068 30.0 2.0 38.0 2.0 40.0 58-59 21.756394954894873 29.0 2.0 37.0 2.0 40.0 60-61 21.48571514258425 28.0 2.0 37.0 2.0 40.0 62-63 21.043610433219655 28.0 2.0 36.5 2.0 39.0 64-65 20.70822114448636 27.5 2.0 36.0 2.0 39.0 66-67 20.360474520063864 26.5 2.0 36.0 2.0 39.0 68-69 19.912741772577853 26.0 2.0 35.0 2.0 38.5 70-71 19.57160117989542 26.0 2.0 35.0 2.0 38.0 72-73 19.168872404873092 25.0 2.0 34.0 2.0 38.0 74-75 18.70454578956973 22.5 2.0 34.0 2.0 37.0 76-77 18.278780791676684 22.0 2.0 34.0 2.0 36.5 78-79 17.89522430059986 20.5 2.0 33.5 2.0 36.0 80-81 17.456954302180712 19.5 2.0 33.0 2.0 35.5 82-83 16.980113536722797 17.5 2.0 32.0 2.0 35.0 84-85 16.56493338816333 12.5 2.0 32.0 2.0 35.0 86-87 16.045947146662954 7.5 2.0 31.5 2.0 34.5 88-89 15.60063320511993 4.5 2.0 31.0 2.0 34.0 90-91 15.080313151116787 2.0 2.0 31.0 2.0 34.0 92-93 14.481431337375774 2.0 2.0 31.0 2.0 34.0 94-95 13.866591300838259 2.0 2.0 30.5 2.0 34.0 96-97 13.139762353650422 2.0 2.0 30.0 2.0 34.0 98-99 12.359379868682499 2.0 2.0 29.0 2.0 34.0 100-101 11.187989536891557 2.0 2.0 27.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.954066864868583 1101 2 9.62949282405253 1101 3 8.888301565285396 1101 4 11.20755117218457 1101 5 11.919742480165056 1101 6 11.037887028564189 1101 7 10.633665632929258 1101 8 10.120762171549146 1101 9 9.83590075062951 1101 10-11 9.023591343938502 1101 12-13 8.998669571818947 1101 14-15 9.734230899815895 1101 16-17 11.094197837378182 1101 18-19 12.17417398779153 1101 20-21 12.216868658922866 1101 22-23 12.391437683726 1101 24-25 12.320199797918264 1101 26-27 12.474802614726034 1101 28-29 12.508079485470308 1101 30-31 11.89493565131081 1101 32-33 11.627510416631111 1101 34-35 11.783720022725035 1101 36-37 12.162313926349036 1101 38-39 11.776142362767057 1101 40-41 11.404521193628693 1101 42-43 11.266041813896308 1101 44-45 11.162994387036452 1101 46-47 11.062385649377187 1101 48-49 11.305979943253256 1101 50-51 13.102991058645177 1101 52-53 11.967013329204764 1101 54-55 11.516905206967081 1101 56-57 11.318435781641181 1101 58-59 11.462021902670845 1101 60-61 11.257431398555713 1101 62-63 11.277091117290784 1101 64-65 10.995119988740381 1101 66-67 10.834026595159774 1101 68-69 10.884231029081626 1101 70-71 10.494944923943724 1101 72-73 10.466426677944135 1101 74-75 10.464067614651832 1101 76-77 10.406453507792186 1101 78-79 9.787949770109385 1101 80-81 9.662869143940856 1101 82-83 9.26686748187566 1101 84-85 8.98630072222421 1101 86-87 8.832903442100188 1101 88-89 8.611526444533538 1101 90-91 8.405706294554498 1101 92-93 8.200824356458947 1101 94-95 7.820720999624756 1101 96-97 7.713816292844385 1101 98-99 7.518539717203845 1101 100-101 6.849451352463655 1102 1 7.894674593848926 1102 2 9.229091375005808 1102 3 8.567424949386545 1102 4 10.74435987793849 1102 5 11.568218517660064 1102 6 10.497973072040647 1102 7 10.289595706469896 1102 8 9.570183894257823 1102 9 9.404884857178647 1102 10-11 8.582262402908523 1102 12-13 8.64872523137733 1102 14-15 9.162547125410267 1102 16-17 9.99228432123796 1102 18-19 11.30784941263067 1102 20-21 11.532805831746327 1102 22-23 11.744773271000778 1102 24-25 11.446559439233638 1102 26-27 11.734489149542046 1102 28-29 11.685845970106246 1102 30-31 11.635649215707588 1102 32-33 11.3859993956589 1102 34-35 11.036982929879244 1102 36-37 11.214644140441138 1102 38-39 11.044762161793653 1102 40-41 10.732553616639652 1102 42-43 10.518686222926355 1102 44-45 10.463864776826313 1102 46-47 10.324282853618026 1102 48-49 9.977879712178563 1102 50-51 11.958481634695243 1102 52-53 11.214327581510656 1102 54-55 10.755368977846263 1102 56-57 10.615153850265049 1102 58-59 10.683432209159072 1102 60-61 10.48944011595195 1102 62-63 10.560505307082497 1102 64-65 10.40184277334654 1102 66-67 10.320562212637089 1102 68-69 9.880377042304936 1102 70-71 9.633061935141438 1102 72-73 9.643834296575555 1102 74-75 9.28959904848761 1102 76-77 9.172492698533002 1102 78-79 8.835635817297891 1102 80-81 8.520424333166481 1102 82-83 8.748482005582542 1102 84-85 8.463070291230673 1102 86-87 8.351907732884785 1102 88-89 8.265879321493642 1102 90-91 8.05199043375275 1102 92-93 7.631156791027854 1102 94-95 7.504839191083514 1102 96-97 7.301516057720185 1102 98-99 6.8625221153694245 1102 100-101 6.054898080002209 1103 1 7.9383535477372185 1103 2 9.423047943765678 1103 3 8.716987910069626 1103 4 11.042918947657729 1103 5 11.657603962536427 1103 6 10.835100967272592 1103 7 10.374971078479192 1103 8 9.831067559414052 1103 9 9.69387144162086 1103 10-11 8.93934539589348 1103 12-13 8.74742213315945 1103 14-15 9.614873477550649 1103 16-17 10.652412686506764 1103 18-19 11.669081507828718 1103 20-21 11.895466616036973 1103 22-23 12.217466441800862 1103 24-25 12.230993251253167 1103 26-27 12.096328659514686 1103 28-29 11.68308592278466 1103 30-31 11.65750262655056 1103 32-33 11.686356247968455 1103 34-35 11.428806951549248 1103 36-37 11.70413023967403 1103 38-39 11.490368555781142 1103 40-41 11.231343225295266 1103 42-43 10.877522513239448 1103 44-45 11.011658978714262 1103 46-47 10.99097315630566 1103 48-49 11.006420878386031 1103 50-51 12.854433393266813 1103 52-53 11.867806999903529 1103 54-55 11.3157859502846 1103 56-57 11.250319068115882 1103 58-59 11.133038354607091 1103 60-61 10.967694254165082 1103 62-63 11.027097700907735 1103 64-65 10.872904336201533 1103 66-67 10.7083750260853 1103 68-69 10.675361705726964 1103 70-71 10.325956121526634 1103 72-73 10.112362057340214 1103 74-75 10.073742312913168 1103 76-77 9.68829208837273 1103 78-79 9.52536649258004 1103 80-81 9.299248873590376 1103 82-83 9.145315625128479 1103 84-85 8.650593733725268 1103 86-87 8.624526217987176 1103 88-89 8.310904909292589 1103 90-91 8.015651548121987 1103 92-93 7.76053159659836 1103 94-95 7.5132673420179845 1103 96-97 6.803855301297329 1103 98-99 6.367473087656238 1103 100-101 5.847018862752909 1104 1 7.833618453445826 1104 2 9.459214689090437 1104 3 8.66311387608933 1104 4 10.77406865858924 1104 5 11.65539539077075 1104 6 10.787117687033476 1104 7 10.334002502387314 1104 8 9.75027525119232 1104 9 9.355431477410296 1104 10-11 8.749363364351584 1104 12-13 8.575972071216203 1104 14-15 9.433303253080108 1104 16-17 10.59642256547162 1104 18-19 11.70070789909374 1104 20-21 11.947102274208572 1104 22-23 12.012914534833723 1104 24-25 11.986188372484946 1104 26-27 12.032359658177501 1104 28-29 11.678594115516649 1104 30-31 11.408744871354209 1104 32-33 11.551907751812863 1104 34-35 11.456208235642606 1104 36-37 11.70065668599971 1104 38-39 11.09567945189347 1104 40-41 10.84530282453228 1104 42-43 10.49333703008211 1104 44-45 10.580937708582706 1104 46-47 10.681832409938682 1104 48-49 10.483513245109723 1104 50-51 12.34833984912979 1104 52-53 11.39402257469754 1104 54-55 11.136367454817275 1104 56-57 11.038239335848363 1104 58-59 11.018958617423447 1104 60-61 10.850109008456862 1104 62-63 10.883644082143842 1104 64-65 10.701611590444148 1104 66-67 10.478685406003887 1104 68-69 10.304654409697232 1104 70-71 10.054887428430849 1104 72-73 9.837993104181106 1104 74-75 9.48053185826939 1104 76-77 9.226271042416741 1104 78-79 9.248767766841148 1104 80-81 8.975364958187154 1104 82-83 8.48524118261771 1104 84-85 8.217564032920992 1104 86-87 8.06669285515706 1104 88-89 7.885894646869524 1104 90-91 7.409771917126761 1104 92-93 7.171222706267406 1104 94-95 6.849207146454299 1104 96-97 6.487519797492247 1104 98-99 6.466419130662553 1104 100-101 5.859765468164587 1105 1 7.742712959780256 1105 2 9.048174297674556 1105 3 8.511931665304726 1105 4 10.640739548755295 1105 5 10.74516197477164 1105 6 10.44082087589852 1105 7 9.89543022560131 1105 8 9.509990324902631 1105 9 9.443750590262194 1105 10-11 8.656417879477456 1105 12-13 8.37943911315924 1105 14-15 9.222340791378645 1105 16-17 10.481709144256698 1105 18-19 11.227050746371997 1105 20-21 11.572397816653503 1105 22-23 11.732284425629604 1105 24-25 11.589744066910256 1105 26-27 11.83158034053098 1105 28-29 11.681526269714556 1105 30-31 11.218689551713211 1105 32-33 11.137054217030915 1105 34-35 11.188879996751645 1105 36-37 11.322533859256673 1105 38-39 10.982228057400587 1105 40-41 11.05498070684509 1105 42-43 10.5441042102935 1105 44-45 10.386479390831216 1105 46-47 10.488835607496764 1105 48-49 10.378963490646754 1105 50-51 12.292729893808673 1105 52-53 11.072842155824812 1105 54-55 10.917896697834966 1105 56-57 10.837455917717005 1105 58-59 10.753111371145046 1105 60-61 10.685174796635657 1105 62-63 10.531701415942994 1105 64-65 10.27769854696589 1105 66-67 9.969230713081 1105 68-69 9.768081877861064 1105 70-71 9.84994294079463 1105 72-73 9.665234654722045 1105 74-75 9.387139420547555 1105 76-77 9.173687449932768 1105 78-79 8.709241553261652 1105 80-81 8.398121459915917 1105 82-83 8.467952000844857 1105 84-85 8.049603970823247 1105 86-87 7.732660799206764 1105 88-89 7.509132566021087 1105 90-91 7.163370810159876 1105 92-93 7.163747522401666 1105 94-95 6.935405591058386 1105 96-97 6.474831974784088 1105 98-99 5.959286234768941 1105 100-101 5.431037122825034 1106 1 8.004498054113121 1106 2 9.541583576589133 1106 3 8.91788723131819 1106 4 10.786485898710186 1106 5 11.535211103418021 1106 6 10.58994390432051 1106 7 9.833598477780917 1106 8 9.419506170804453 1106 9 9.223934501078315 1106 10-11 8.58948691768283 1106 12-13 8.384532831303776 1106 14-15 9.063808088342338 1106 16-17 10.377029058538042 1106 18-19 11.503568328141807 1106 20-21 11.565108412775981 1106 22-23 11.758827043956853 1106 24-25 11.564683185330324 1106 26-27 11.728922516586426 1106 28-29 11.789265904172684 1106 30-31 11.295293784397685 1106 32-33 11.49701416668097 1106 34-35 11.418011619022863 1106 36-37 11.728141763155378 1106 38-39 11.386450096247554 1106 40-41 11.263852767186851 1106 42-43 10.918838476750864 1106 44-45 10.587936457409732 1106 46-47 10.582111643073912 1106 48-49 10.631576859435654 1106 50-51 12.845426962048148 1106 52-53 11.888491673331679 1106 54-55 11.3384221154366 1106 56-57 11.125249618926244 1106 58-59 10.957985113617795 1106 60-61 10.861379340017702 1106 62-63 10.969272861728872 1106 64-65 10.909892791570204 1106 66-67 10.711299314876793 1106 68-69 10.578431583610275 1106 70-71 10.158053808370376 1106 72-73 10.02910163040361 1106 74-75 9.909325779654026 1106 76-77 9.906570281286516 1106 78-79 9.432439307869195 1106 80-81 8.999378356164424 1106 82-83 8.853915265090865 1106 84-85 8.517752686346073 1106 86-87 8.335115073903802 1106 88-89 8.00200121813425 1106 90-91 8.017325548134998 1106 92-93 7.688217805839924 1106 94-95 7.465932787680446 1106 96-97 7.127160802079878 1106 98-99 6.611156058511577 1106 100-101 5.781764282156873 1107 1 7.494160672844643 1107 2 8.675315868252351 1107 3 8.272249921561762 1107 4 10.263870833836176 1107 5 11.057464676537442 1107 6 10.01856635380685 1107 7 9.891395774228158 1107 8 8.655304738577016 1107 9 6.206148361835087 1107 10-11 8.000665786368824 1107 12-13 7.9392696562018905 1107 14-15 8.454731106945399 1107 16-17 9.78375259825205 1107 18-19 11.310373229365783 1107 20-21 11.529833583858622 1107 22-23 11.114348908103825 1107 24-25 11.214486340965038 1107 26-27 11.111629305596086 1107 28-29 11.365110681641088 1107 30-31 11.08597774666184 1107 32-33 11.23709000752693 1107 34-35 11.480590559551985 1107 36-37 11.837134527526487 1107 38-39 11.370642436346937 1107 40-41 10.993142000319441 1107 42-43 10.618709999229154 1107 44-45 10.13434076286551 1107 46-47 10.518255686881666 1107 48-49 10.713961595917063 1107 50-51 12.410918632218685 1107 52-53 11.414070473981909 1107 54-55 10.90331019576847 1107 56-57 10.680241164116236 1107 58-59 10.89814860638548 1107 60-61 10.620651386255538 1107 62-63 10.919975070325549 1107 64-65 10.809912076648356 1107 66-67 10.702104450335842 1107 68-69 10.433556986279395 1107 70-71 10.072274939518838 1107 72-73 9.968374843678365 1107 74-75 9.999462517479593 1107 76-77 9.842930247616259 1107 78-79 9.49817835262051 1107 80-81 9.017740047391285 1107 82-83 9.09716621196337 1107 84-85 8.855544797342043 1107 86-87 8.608657146826227 1107 88-89 8.494896156670308 1107 90-91 8.488695794321853 1107 92-93 7.971933171329402 1107 94-95 7.843255584298193 1107 96-97 7.359797299876579 1107 98-99 6.919930334534845 1107 100-101 6.397792168545413 1108 1 7.0806721870005696 1108 2 8.709462338321249 1108 3 8.291736009770975 1108 4 9.93800169119962 1108 5 11.497054716557322 1108 6 10.06571526673687 1108 7 9.356441125879485 1108 8 6.656727758229437 1108 9 7.7918953164037 1108 10-11 6.608846895466794 1108 12-13 5.55623269777297 1108 14-15 7.734877892955094 1108 16-17 9.020977197651334 1108 18-19 9.072309050514999 1108 20-21 9.412892190876928 1108 22-23 10.201143032805273 1108 24-25 9.704620478625642 1108 26-27 8.234181862905363 1108 28-29 10.20369665579867 1108 30-31 10.339934562227214 1108 32-33 10.806727946658384 1108 34-35 10.422306448180493 1108 36-37 10.10688527509943 1108 38-39 10.11964869107453 1108 40-41 10.318807731952813 1108 42-43 9.539994335017205 1108 44-45 9.127544070341276 1108 46-47 9.992557203719016 1108 48-49 10.336785189278533 1108 50-51 12.22532441809356 1108 52-53 11.04108371241583 1108 54-55 10.47624450773236 1108 56-57 10.434077626385207 1108 58-59 10.368586278703425 1108 60-61 10.51090086159876 1108 62-63 10.535680564630365 1108 64-65 10.263259795219266 1108 66-67 9.65614717436284 1108 68-69 9.902284634378788 1108 70-71 9.731210094706004 1108 72-73 9.564368654325225 1108 74-75 9.300297852950209 1108 76-77 8.806423116763824 1108 78-79 8.722506823023352 1108 80-81 8.818627253739425 1108 82-83 8.859111678523693 1108 84-85 8.534494823609714 1108 86-87 8.248800683404093 1108 88-89 8.216734717780739 1108 90-91 8.19356454063918 1108 92-93 7.764270470927228 1108 94-95 7.483043098034864 1108 96-97 7.090999733263764 1108 98-99 6.821170219981655 1108 100-101 6.211286570241635 1109 1 6.738471980876113 1109 2 8.380549564061575 1109 3 8.185209128252378 1109 4 9.502173751427076 1109 5 10.543968764580562 1109 6 9.475849893680849 1109 7 8.326343550701022 1109 8 8.791707191618542 1109 9 7.827234616375019 1109 10-11 6.343752600731516 1109 12-13 6.7772169267640585 1109 14-15 7.932454970274474 1109 16-17 6.9702482975797935 1109 18-19 6.343557068171332 1109 20-21 8.709023013990976 1109 22-23 10.07523010707337 1109 24-25 9.467755783484229 1109 26-27 10.034980060429515 1109 28-29 10.490774285096808 1109 30-31 8.076075180836298 1109 32-33 6.882014554374567 1109 34-35 8.720771449583907 1109 36-37 7.214352091986967 1109 38-39 7.643217245789398 1109 40-41 8.667137543302228 1109 42-43 7.390615436626263 1109 44-45 8.00002082459265 1109 46-47 7.6255602955858315 1109 48-49 8.327622974745843 1109 50-51 10.064055386393957 1109 52-53 10.343215804055216 1109 54-55 10.283059470220067 1109 56-57 9.166290989203969 1109 58-59 9.29777115732266 1109 60-61 9.572525067297171 1109 62-63 9.659726496946284 1109 64-65 9.634071555400755 1109 66-67 9.300391513470306 1109 68-69 9.238518275269133 1109 70-71 8.791511428403684 1109 72-73 8.087820985753442 1109 74-75 7.107752207209263 1109 76-77 7.079893127227194 1109 78-79 8.01643038585966 1109 80-81 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56-57 -18.99233131632555 2313 58-59 -18.73760841156206 2313 60-61 -18.489564904586615 2313 62-63 -18.057705555537314 2313 64-65 -17.72230145303411 2313 66-67 -17.406731260662777 2313 68-69 -16.984068416389725 2313 70-71 -16.667485759974948 2313 72-73 -16.285421750891356 2313 74-75 -15.82336306926684 2313 76-77 -15.430884754684708 2313 78-79 -15.064638030260301 2313 80-81 -14.655522722972268 2313 82-83 -14.206561353614173 2313 84-85 -13.806213140992055 2313 86-87 -13.310118739045839 2313 88-89 -12.881541947333375 2313 90-91 -12.39027337272831 2313 92-93 -11.841458453152883 2313 94-95 -11.23842872311092 2313 96-97 -10.539880564826596 2313 98-99 -9.790147898322955 2313 100-101 -8.701149386907877 2314 1 -19.97661705380055 2314 2 -18.529009948590723 2314 3 -19.298389747099076 2314 4 -20.749934964599166 2314 5 -20.050220551258235 2314 6 -21.182538110067977 2314 7 -21.472156917902538 2314 8 -22.039126922720726 2314 9 -23.547648232734634 2314 10-11 -24.06721272260494 2314 12-13 -24.06533227660989 2314 14-15 -24.08277464547061 2314 16-17 -22.805524898296497 2314 18-19 -21.607942463225104 2314 20-21 -21.275044464921866 2314 22-23 -20.89899669705034 2314 24-25 -20.759282642007804 2314 26-27 -20.48915302298192 2314 28-29 -20.344277261294945 2314 30-31 -20.423456215602315 2314 32-33 -20.359550581520466 2314 34-35 -20.071871474502316 2314 36-37 -19.57266758936261 2314 38-39 -19.51845098288914 2314 40-41 -19.476255146482213 2314 42-43 -19.361961882961367 2314 44-45 -18.97748620446077 2314 46-47 -18.648104184264216 2314 48-49 -18.46689436358593 2314 50-51 -15.994051455218038 2314 52-53 -18.09352271515753 2314 54-55 -18.96553471909577 2314 56-57 -18.99233131632555 2314 58-59 -18.73760841156206 2314 60-61 -18.489564904586615 2314 62-63 -18.057705555537314 2314 64-65 -17.72230145303411 2314 66-67 -17.406731260662777 2314 68-69 -16.984068416389725 2314 70-71 -16.667485759974948 2314 72-73 -16.285421750891356 2314 74-75 -15.82336306926684 2314 76-77 -15.430884754684708 2314 78-79 -15.064638030260301 2314 80-81 -14.655522722972268 2314 82-83 -14.206561353614173 2314 84-85 -13.806213140992055 2314 86-87 -13.310118739045839 2314 88-89 -12.881541947333375 2314 90-91 -12.39027337272831 2314 92-93 -11.841458453152883 2314 94-95 -11.23842872311092 2314 96-97 -10.539880564826596 2314 98-99 -9.790147898322955 2314 100-101 -8.701149386907877 2315 1 -19.97661705380055 2315 2 -18.529009948590723 2315 3 -19.298389747099076 2315 4 -20.749934964599166 2315 5 -20.050220551258235 2315 6 -21.182538110067977 2315 7 -21.472156917902538 2315 8 -22.039126922720726 2315 9 -23.547648232734634 2315 10-11 -24.06721272260494 2315 12-13 -24.06533227660989 2315 14-15 -24.08277464547061 2315 16-17 -22.805524898296497 2315 18-19 -21.607942463225104 2315 20-21 -21.275044464921866 2315 22-23 -20.89899669705034 2315 24-25 -20.759282642007804 2315 26-27 -20.48915302298192 2315 28-29 -20.344277261294945 2315 30-31 -20.423456215602315 2315 32-33 -20.359550581520466 2315 34-35 -20.071871474502316 2315 36-37 -19.57266758936261 2315 38-39 -19.51845098288914 2315 40-41 -19.476255146482213 2315 42-43 -19.361961882961367 2315 44-45 -18.97748620446077 2315 46-47 -18.648104184264216 2315 48-49 -18.46689436358593 2315 50-51 -15.994051455218038 2315 52-53 -18.09352271515753 2315 54-55 -18.96553471909577 2315 56-57 -18.99233131632555 2315 58-59 -18.73760841156206 2315 60-61 -18.489564904586615 2315 62-63 -18.057705555537314 2315 64-65 -17.72230145303411 2315 66-67 -17.406731260662777 2315 68-69 -16.984068416389725 2315 70-71 -16.667485759974948 2315 72-73 -16.285421750891356 2315 74-75 -15.82336306926684 2315 76-77 -15.430884754684708 2315 78-79 -15.064638030260301 2315 80-81 -14.655522722972268 2315 82-83 -14.206561353614173 2315 84-85 -13.806213140992055 2315 86-87 -13.310118739045839 2315 88-89 -12.881541947333375 2315 90-91 -12.39027337272831 2315 92-93 -11.841458453152883 2315 94-95 -11.23842872311092 2315 96-97 -10.539880564826596 2315 98-99 -9.790147898322955 2315 100-101 -8.701149386907877 2316 1 -19.97661705380055 2316 2 -18.529009948590723 2316 3 -19.298389747099076 2316 4 -20.749934964599166 2316 5 -20.050220551258235 2316 6 -21.182538110067977 2316 7 -21.472156917902538 2316 8 -22.039126922720726 2316 9 -23.547648232734634 2316 10-11 -24.06721272260494 2316 12-13 -24.06533227660989 2316 14-15 -24.08277464547061 2316 16-17 -22.805524898296497 2316 18-19 -21.607942463225104 2316 20-21 -21.275044464921866 2316 22-23 -20.89899669705034 2316 24-25 -20.759282642007804 2316 26-27 -20.48915302298192 2316 28-29 -20.344277261294945 2316 30-31 -20.423456215602315 2316 32-33 -20.359550581520466 2316 34-35 -20.071871474502316 2316 36-37 -19.57266758936261 2316 38-39 -19.51845098288914 2316 40-41 -19.476255146482213 2316 42-43 -19.361961882961367 2316 44-45 -18.97748620446077 2316 46-47 -18.648104184264216 2316 48-49 -18.46689436358593 2316 50-51 -15.994051455218038 2316 52-53 -18.09352271515753 2316 54-55 -18.96553471909577 2316 56-57 -18.99233131632555 2316 58-59 -18.73760841156206 2316 60-61 -18.489564904586615 2316 62-63 -18.057705555537314 2316 64-65 -17.72230145303411 2316 66-67 -17.406731260662777 2316 68-69 -16.984068416389725 2316 70-71 -16.667485759974948 2316 72-73 -16.285421750891356 2316 74-75 -15.82336306926684 2316 76-77 -15.430884754684708 2316 78-79 -15.064638030260301 2316 80-81 -14.655522722972268 2316 82-83 -14.206561353614173 2316 84-85 -13.806213140992055 2316 86-87 -13.310118739045839 2316 88-89 -12.881541947333375 2316 90-91 -12.39027337272831 2316 92-93 -11.841458453152883 2316 94-95 -11.23842872311092 2316 96-97 -10.539880564826596 2316 98-99 -9.790147898322955 2316 100-101 -8.701149386907877 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 424130.0 3 15253.0 4 73989.0 5 33752.0 6 15191.0 7 16742.0 8 15985.0 9 14187.0 10 15073.0 11 16852.0 12 18652.0 13 19953.0 14 21047.0 15 22497.0 16 24200.0 17 24198.0 18 23327.0 19 23995.0 20 25783.0 21 27813.0 22 30463.0 23 33551.0 24 36906.0 25 40528.0 26 45391.0 27 50498.0 28 56198.0 29 64048.0 30 71821.0 31 81002.0 32 91817.0 33 103223.0 34 113655.0 35 117201.0 36 98945.0 37 50825.0 38 8508.0 39 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.45727119975546 21.012424290576135 14.422298248065903 23.108006261602505 2 14.428642509404273 20.841178938331105 42.764389338990064 21.965789213274554 3 18.400209254350983 26.26328939077863 30.718577379656196 24.617923975214183 4 11.695359177047479 17.616745789564703 40.75998426839677 29.927910764991044 5 12.16996160028836 38.240851363175224 36.59018699874714 12.99900003778927 6 28.66708622939592 39.55784610950323 18.60022503017811 13.174842630922742 7 25.169293754796712 33.31252858481236 23.777416988401896 17.740760671989033 8 23.53739253766625 37.55477313161725 21.228585291812337 17.679249038904164 9 24.553762510586758 17.181566293587842 22.183695028550094 36.08097616727531 10-11 23.180375979368666 27.81059470663973 29.754469032739138 19.254560281252466 12-13 23.84992538399025 25.342601104915413 29.94334397639782 20.864129534696517 14-15 21.1030097888448 27.192525536859186 27.74414528739172 23.960319386904292 16-17 20.399619986542262 30.220544445332457 28.48808655231888 20.891749015806393 18-19 20.114055531733868 29.06241672742324 30.598825357877637 20.224702382965255 20-21 20.924013167147695 28.38416983523007 30.663660221191137 20.028156776431096 22-23 20.3816844495143 28.26485348830144 30.36955224787531 20.983909814308955 24-25 20.391299406469297 28.37412620033485 30.711100330267012 20.52347406292884 26-27 20.343424855740842 28.649886215696363 30.43379618278697 20.57289274577582 28-29 20.318372077790595 28.696411406653716 30.500799138718975 20.484417376836713 30-31 20.654800432966365 28.46026336974022 30.459095285235627 20.425840912057787 32-33 20.429967417958878 28.653624894801847 30.29837653985574 20.61803114738353 34-35 20.581297769776004 28.683386402660705 30.301743906431767 20.43357192113152 36-37 20.625667992471865 28.495193516632227 30.284843389048007 20.594295101847905 38-39 20.51671499415605 28.794537329193542 30.352651490232986 20.336096186417425 40-41 20.762356158440184 28.613894905683324 30.252201379827177 20.371547556049315 42-43 20.60833912014754 28.6791856591458 30.121496543180697 20.59097867752596 44-45 20.615135453474675 29.01426514854077 29.83637612877896 20.5342232692056 46-47 20.706656912948063 28.79508684779095 29.617699596808496 20.880556642452493 48-49 20.67943826739833 28.924269911312315 29.579626757263583 20.81666506402577 50-51 20.960129798198302 29.211764832211788 29.10022816522529 20.727877204364617 52-53 20.461174393509623 29.4142299254464 29.264301116154385 20.86029456488959 54-55 20.531220891093742 29.301935131836608 29.33967883946978 20.827165137599867 56-57 20.46602065303739 29.40454573624708 29.375149343287234 20.7542842674283 58-59 20.438916890429056 29.329592886539746 29.523237520232204 20.708252702798998 60-61 20.487112236887416 29.38625424954261 29.441875723217887 20.68475779035209 62-63 20.40319268040589 29.252544955091324 29.341505594487373 21.00275677001541 64-65 20.258103875303032 29.29333989705036 29.44544059590698 21.00311563173963 66-67 20.284821722389623 29.340733151257286 29.21625083476566 21.158194291587428 68-69 20.396950186068363 29.301124490193537 29.228274050582186 21.073651273155917 70-71 20.41753360787405 28.987633505745414 29.374160463256256 21.220672423124277 72-73 20.45017991482392 29.18117515393209 29.200019419175028 21.168625512068953 74-75 20.30739153571487 29.4653856774797 28.930049225663545 21.297173561141882 76-77 20.30299197102424 29.384964311072437 29.110074708694572 21.201969009208753 78-79 20.235382718698084 29.33526025198635 28.988402311169885 21.440954718145676 80-81 20.233672821745177 29.38173959760098 28.938635089074705 21.445952491579135 82-83 20.4179185487771 29.290311461409445 29.09603393457973 21.195736055233724 84-85 20.553498581779788 29.233589886787627 28.93746637605854 21.27544515537404 86-87 20.260904163955367 29.329343311968454 29.040520797022463 21.369231727053716 88-89 20.47829558195787 29.275457793727472 28.889976808815764 21.35626981549889 90-91 20.37769296715529 29.237500418542737 29.05530007776998 21.32950653653199 92-93 20.372137103342723 29.2116166329292 28.83293829137302 21.58330797235506 94-95 20.114366485158353 29.324174182993872 28.92793449203709 21.633524839810686 96-97 20.11640848999259 29.15436538005463 28.96960321623711 21.759622913715667 98-99 20.057696943115616 29.10268359085829 28.7582722280092 22.08134723801689 100-101 20.23947716141395 29.27547556119359 28.60262724703074 21.88242003036171 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 106629.0 1 96935.5 2 65051.0 3 33684.5 4 20973.5 5 16599.0 6 15720.5 7 16032.5 8 16398.0 9 15928.5 10 14396.5 11 12101.5 12 9682.0 13 7338.5 14 5645.5 15 4861.0 16 4760.5 17 5246.0 18 5979.5 19 7053.0 20 8140.5 21 9261.0 22 10721.5 23 12223.5 24 13879.5 25 15843.5 26 18414.5 27 21539.5 28 24797.5 29 28324.5 30 32294.0 31 36179.5 32 40378.0 33 44748.0 34 48822.0 35 52228.5 36 55925.0 37 59606.0 38 61507.5 39 61907.0 40 61657.0 41 60946.5 42 59698.5 43 59500.0 44 59482.0 45 59188.0 46 57966.5 47 55790.0 48 53455.0 49 50631.5 50 46999.0 51 42569.5 52 38465.5 53 34556.0 54 30170.5 55 25417.0 56 20826.0 57 16618.5 58 13177.0 59 10430.0 60 7956.5 61 5824.0 62 4136.0 63 2799.0 64 1792.5 65 1067.5 66 642.0 67 405.5 68 248.5 69 151.5 70 97.5 71 60.5 72 39.0 73 28.5 74 19.5 75 8.5 76 7.5 77 7.0 78 4.5 79 4.0 80 2.0 81 1.5 82 1.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 20.346842081987788 2 27.057840392864712 3 21.100822297441205 4 27.10511347318371 5 30.05353040955262 6 23.108250779116275 7 21.978779978376377 8 18.286701574193575 9 10.693933237194459 10-11 14.072865607715778 12-13 14.215091498826052 14-15 18.125515619686546 16-17 21.134065947471985 18-19 24.842003707632774 20-21 26.81336740383868 22-23 26.385546026951758 24-25 25.08449427716156 26-27 26.400642913892337 28-29 23.559607033624378 30-31 25.23818261946746 32-33 24.319509091934957 34-35 22.5277068540375 36-37 25.30744022369825 38-39 25.43553502198198 40-41 22.675219197140436 42-43 23.432960926511708 44-45 22.319654469364757 46-47 25.30217918734017 48-49 20.971329639255565 50-51 26.62651165840574 52-53 20.19091716383884 54-55 21.282569500323543 56-57 23.311448777584225 58-59 22.602555897875913 60-61 22.137551447598298 62-63 21.97661964943707 64-65 20.5953053256429 66-67 21.067629478679585 68-69 21.99174195201223 70-71 20.589078495170774 72-73 20.425528886647797 74-75 17.99470134850274 76-77 20.033416476345923 78-79 19.20819623716447 80-81 20.19193378922204 82-83 20.95778310602437 84-85 18.12884506781654 86-87 19.57916283949569 88-89 18.44178777640138 90-91 19.540556490568513 92-93 19.493537566595318 94-95 18.655024950528468 96-97 17.58861033918181 98-99 17.018181836665917 100-101 18.61763855206113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1967293.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.78165511785812 #Duplication Level Percentage of deduplicated Percentage of total 1 87.40697560418772 66.23845280131592 2 8.558281261815923 12.971214379691236 3 2.0699871897864135 4.70601165944335 4 0.7584265366948705 2.29899272944169 5 0.3763212950641233 1.4259125298027557 6 0.19941758025333206 0.9067316574717482 7 0.12825065437067973 0.6803332800710932 8 0.09912045026896402 0.6009209421927552 9 0.06836262289327653 0.46625694399454265 >10 0.317279690130174 4.098086904989177 >50 0.012403660919346046 0.6506970952965496 >100 0.004901333856601796 0.6280003556425365 >500 6.80299396701013E-5 0.04474905797566895 >1k 1.360598793402026E-4 0.18178015947259069 >5k 0.0 0.0 >10k+ 6.80299396701013E-5 4.1018595031983 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 79564 4.04433909946307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.0166253832042303E-4 0.0 0.0 0.0 0.0 7 1.0166253832042303E-4 0.0 0.0 5.0831269160211516E-5 0.0 8 1.0166253832042303E-4 0.0 0.0 5.0831269160211516E-5 0.0 9 1.0166253832042303E-4 0.0 0.0 5.0831269160211516E-5 0.0 10-11 1.270781729005288E-4 0.0 0.0 5.0831269160211516E-5 0.0 12-13 1.5249380748063457E-4 0.0 0.0 5.0831269160211516E-5 0.0 14-15 1.5249380748063457E-4 0.0 0.0 5.0831269160211516E-5 0.0 16-17 2.0332507664084606E-4 0.0 0.0 1.0166253832042303E-4 0.0 18-19 2.0332507664084606E-4 0.0 0.0 1.0166253832042303E-4 0.0 20-21 2.0332507664084606E-4 0.0 0.0 1.5249380748063454E-4 0.0 22-23 2.0332507664084606E-4 0.0 0.0 2.0332507664084606E-4 0.0 24-25 2.0332507664084606E-4 0.0 0.0 4.066501532816922E-4 0.0 26-27 2.0332507664084606E-4 0.0 0.0 4.5748142244190365E-4 0.0 28-29 2.0332507664084606E-4 0.0 0.0 6.608064990827497E-4 0.0 30-31 2.0332507664084606E-4 0.0 0.0 0.0015503537093864515 0.0 32-33 2.541563458010576E-4 0.0 0.0 0.0038631764561760755 0.0 34-35 2.541563458010576E-4 0.0 0.0 0.007751768546932257 0.0 36-37 2.541563458010576E-4 0.0 0.0 0.013470286327456053 0.0 38-39 2.541563458010576E-4 0.0 0.0 0.023331552544537087 0.0 40-41 2.7957198038116335E-4 0.0 0.0 0.03255742789711548 0.0 42-43 3.0498761496126914E-4 0.0 0.0 0.04285075990205831 0.0 44-45 3.0498761496126914E-4 0.0 0.0 0.055888980441652567 0.0 46-47 3.0498761496126914E-4 0.0 0.0 0.07169750515047835 0.0 48-49 3.0498761496126914E-4 0.0 0.0 0.0882176676275471 0.0 50-51 3.0498761496126914E-4 0.0 0.0 0.11017677590475847 2.5415634580105758E-5 52-53 3.0498761496126914E-4 0.0 0.0 0.13404205677547779 5.0831269160211516E-5 54-55 3.0498761496126914E-4 0.0 0.0 0.16171968283321295 5.0831269160211516E-5 56-57 3.0498761496126914E-4 0.0 0.0 0.1954716455555934 5.0831269160211516E-5 58-59 3.0498761496126914E-4 0.0 0.0 0.2368991299211658 5.0831269160211516E-5 60-61 3.0498761496126914E-4 0.0 0.0 0.2815798155129917 5.0831269160211516E-5 62-63 3.5581888412148066E-4 0.0 0.0 0.3274804515646627 5.0831269160211516E-5 64-65 3.5581888412148066E-4 0.0 0.0 0.3733810876163337 5.0831269160211516E-5 66-67 3.5581888412148066E-4 0.0 0.0 0.43005795272996955 5.0831269160211516E-5 68-69 3.5581888412148066E-4 0.0 0.0 0.4961894339074048 5.0831269160211516E-5 70-71 3.812345187015864E-4 0.0 0.0 0.5712418028224571 5.0831269160211516E-5 72-73 4.3206578786179786E-4 0.0 0.0 0.6481240974272771 5.0831269160211516E-5 74-75 4.5748142244190365E-4 0.0 0.0 0.7287170746807923 5.0831269160211516E-5 76-77 4.5748142244190365E-4 0.0 0.0 0.8172397299233007 5.0831269160211516E-5 78-79 4.5748142244190365E-4 0.0 0.0 0.924010810794325 5.0831269160211516E-5 80-81 4.5748142244190365E-4 0.0 0.0 1.0417106145347947 5.0831269160211516E-5 82-83 4.5748142244190365E-4 0.0 0.0 1.1695004252035663 5.0831269160211516E-5 84-85 4.5748142244190365E-4 0.0 0.0 1.299145577196686 5.0831269160211516E-5 86-87 4.5748142244190365E-4 0.0 0.0 1.4379657732732238 5.0831269160211516E-5 88-89 5.083126916021152E-4 0.0 0.0 1.591603284309963 5.0831269160211516E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1755 0.0 60.076424 1 GTATCAA 3430 0.0 47.66743 1 ATCAACG 4115 0.0 38.596462 3 TCAACGC 4240 0.0 38.16619 4 CAACGCA 4220 0.0 37.981125 5 TATCAAC 4420 0.0 36.174664 2 AACGCAG 4620 0.0 34.751396 6 ACGCAGA 5225 0.0 30.42735 7 CGCAGAG 5340 0.0 30.011759 8 GCAGAGT 5915 0.0 26.744501 9 GTACATG 3435 0.0 25.20769 1 GAGTACT 3990 0.0 23.744753 12-13 TACATGG 3520 0.0 23.290585 2 ACATGGG 3500 0.0 23.047081 3 CAGAGTA 5615 0.0 22.496298 10-11 GTACTAG 500 0.0 22.411118 1 GCGTGCG 455 0.0 22.274666 9 AGAGTAC 5350 0.0 22.022251 10-11 GGCCGCG 145 3.225606E-5 21.99151 7 AACGTGC 145 3.7706035E-5 21.551044 8 >>END_MODULE