FastQCFastQC Report
Sat 18 Jun 2016
SRR1931044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967293
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54880.2789620051512408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.25964612287036043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33270.1691156324960237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.061.270371
GTATCAA57500.039.7595331
ATCAACG73150.030.664543
CAACGCA73900.030.4300355
TATCAAC74900.030.0153482
TCAACGC75200.029.89184
AACGCAG76600.029.356696
GTGGTAT14550.029.0730821
TGGTATC14850.026.8854242
CGCAGAG88350.025.2913428
ACGCAGA88750.025.2842627
GCAGAGT97750.022.0341649
CACCGTA1100.00289877421.5642747
GAGTACT74350.021.24813512-13
CAGAGTA96100.020.95616710-11
TAACGCA1604.972588E-520.7915484
GTACTTT79800.019.61862814-15
GAGTACA41350.019.5405791
AGAGTAC93000.019.43567310-11
TATACCG1250.006074904518.9770455
Produced by FastQC (version 0.11.5)