FastQCFastQC Report
Sat 18 Jun 2016
SRR1931043_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931043_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418551
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1051774.348760890301672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.054.782211
GTATCAA38200.048.8077961
ATCAACG44650.040.4898073
TCAACGC45350.040.1929974
CAACGCA46150.039.486515
TATCAAC48650.037.8968162
AACGCAG49850.036.5413446
ACGCAGA57600.031.3706047
CGCAGAG59400.029.8401228
GTACATG40300.027.707841
GCAGAGT63450.026.8696379
TACATGG42250.025.9400082
GTGGTAT10350.024.7446211
ACATGGG43600.024.3707393
AGAGTAC58300.022.21658110-11
GAGTACT42750.021.17208512-13
CAGAGTA62400.021.0507410-11
TGGTATC11250.020.0301042
CATGGGG26600.019.3080164
AGTACTT47850.019.16636712-13
Produced by FastQC (version 0.11.5)