##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931043_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2418551 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.97034381329978 30.0 2.0 31.0 2.0 33.0 2 20.575345320400523 28.0 2.0 31.0 2.0 33.0 3 21.30228347469208 28.0 2.0 31.0 2.0 34.0 4 22.846836804351035 32.0 2.0 35.0 2.0 37.0 5 22.18182705264433 32.0 2.0 35.0 2.0 37.0 6 23.22231575848514 32.0 2.0 35.0 2.0 37.0 7 23.455435093161153 32.0 2.0 35.0 2.0 37.0 8 23.945473550071924 32.0 2.0 35.0 2.0 37.0 9 25.373278876484306 33.0 10.0 37.0 2.0 39.0 10-11 25.81752689937074 33.5 10.0 37.0 2.0 39.0 12-13 25.76479594600238 34.0 6.0 37.0 2.0 39.0 14-15 25.782839807802276 34.0 2.0 38.0 2.0 40.0 16-17 24.590710305468026 32.5 2.0 38.0 2.0 40.0 18-19 23.48647433938751 32.0 2.0 38.0 2.0 40.0 20-21 23.150743151581256 31.5 2.0 38.0 2.0 40.0 22-23 22.780430513973037 30.0 2.0 38.0 2.0 40.0 24-25 22.6269051179818 30.0 2.0 38.0 2.0 40.0 26-27 22.355558555515266 30.0 2.0 38.0 2.0 40.0 28-29 22.186880284930936 30.0 2.0 38.0 2.0 40.0 30-31 22.227404962723547 30.0 2.0 37.5 2.0 40.0 32-33 22.123084648618118 30.0 2.0 37.5 2.0 40.0 34-35 21.822260518798238 29.0 2.0 37.0 2.0 40.0 36-37 21.352424447530773 27.0 2.0 37.0 2.0 40.0 38-39 21.27499110004296 27.0 2.0 37.0 2.0 39.0 40-41 21.18418011445696 27.0 2.0 37.0 2.0 39.0 42-43 21.06532899244217 27.0 2.0 37.0 2.0 39.0 44-45 20.686139138682627 26.0 2.0 36.5 2.0 39.0 46-47 20.346486801394718 25.0 2.0 36.0 2.0 39.0 48-49 20.132256669385924 24.5 2.0 36.0 2.0 39.0 50-51 17.632567599360115 12.0 2.0 33.5 2.0 38.5 52-53 19.6771281647565 24.5 2.0 35.5 2.0 38.5 54-55 20.542441528005817 26.5 2.0 37.0 2.0 39.0 56-57 20.549231750746628 26.5 2.0 37.0 2.0 39.0 58-59 20.28578082496503 26.0 2.0 37.0 2.0 39.0 60-61 19.998176594167333 26.0 2.0 36.0 2.0 39.0 62-63 19.56078060789291 24.0 2.0 36.0 2.0 39.0 64-65 19.22873117002701 23.0 2.0 35.0 2.0 39.0 66-67 18.88586430470145 22.0 2.0 35.0 2.0 38.5 68-69 18.444066922715294 21.0 2.0 34.0 2.0 38.0 70-71 18.099615844363008 20.0 2.0 34.0 2.0 38.0 72-73 17.702407143781546 18.0 2.0 34.0 2.0 37.0 74-75 17.25037718865552 14.0 2.0 33.5 2.0 36.5 76-77 16.84349885530634 10.0 2.0 33.0 2.0 36.0 78-79 16.473229011916636 7.5 2.0 32.0 2.0 35.5 80-81 16.038946046620477 7.0 2.0 32.0 2.0 35.0 82-83 15.584471859390188 2.0 2.0 31.5 2.0 35.0 84-85 15.201183270478893 2.0 2.0 31.0 2.0 34.5 86-87 14.708314399820388 2.0 2.0 31.0 2.0 34.0 88-89 14.290435678222208 2.0 2.0 31.0 2.0 34.0 90-91 13.806487438139614 2.0 2.0 30.0 2.0 34.0 92-93 13.251035020555697 2.0 2.0 30.0 2.0 34.0 94-95 12.67239289144616 2.0 2.0 29.0 2.0 34.0 96-97 12.004736720457828 2.0 2.0 29.0 2.0 34.0 98-99 11.28637973728898 2.0 2.0 28.0 2.0 34.0 100-101 10.228548829443746 2.0 2.0 25.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.071670444921377 1101 2 9.645006255229873 1101 3 8.988404857019432 1101 4 11.038000462762632 1101 5 11.847406054968129 1101 6 10.919504785767064 1101 7 10.665016846306859 1101 8 9.927560660472029 1101 9 9.825640071704829 1101 10-11 9.28696043136378 1101 12-13 9.141896258156155 1101 14-15 9.974539781093966 1101 16-17 10.88638759100007 1101 18-19 12.063223955959 1101 20-21 11.918547107338956 1101 22-23 12.225307372927578 1101 24-25 12.191515457958172 1101 26-27 12.177542891874477 1101 28-29 12.05048046757366 1101 30-31 11.607582398254777 1101 32-33 11.658703971889619 1101 34-35 11.525960057118638 1101 36-37 11.933521732538129 1101 38-39 11.633436290888543 1101 40-41 11.262218581218821 1101 42-43 10.772670308510673 1101 44-45 10.984746980553275 1101 46-47 10.823128803494548 1101 48-49 11.044264560882091 1101 50-51 12.9793490981451 1101 52-53 11.983555481577831 1101 54-55 11.579437177443388 1101 56-57 11.624201467425227 1101 58-59 11.354636913669808 1101 60-61 11.026390009009699 1101 62-63 11.196265275820647 1101 64-65 11.099819573241238 1101 66-67 10.857861444110831 1101 68-69 10.714088468301195 1101 70-71 10.391361356503019 1101 72-73 10.326354360590013 1101 74-75 10.085242358408625 1101 76-77 9.837727092477163 1101 78-79 9.321671252693882 1101 80-81 9.407029453820916 1101 82-83 9.109272958448846 1101 84-85 8.917591747286785 1101 86-87 8.603398648247826 1101 88-89 8.441931504375646 1101 90-91 8.220111026757584 1101 92-93 8.168233885315892 1101 94-95 7.678039602914277 1101 96-97 7.576363926702365 1101 98-99 7.436751291424356 1101 100-101 6.735760176879001 1102 1 7.863151255084478 1102 2 9.259677138437848 1102 3 8.715249248136612 1102 4 10.580772242996161 1102 5 11.335265415657926 1102 6 10.42231792299506 1102 7 10.116679742126163 1102 8 9.545037341317933 1102 9 9.104843662359936 1102 10-11 8.54192063802111 1102 12-13 8.592568947541512 1102 14-15 9.119210919592028 1102 16-17 10.033233162833906 1102 18-19 11.416889203638252 1102 20-21 11.498004392520333 1102 22-23 11.762902109484163 1102 24-25 11.415955893993644 1102 26-27 11.617054218776627 1102 28-29 11.4103690494053 1102 30-31 11.40719443709716 1102 32-33 11.267821737207822 1102 34-35 11.10724537889584 1102 36-37 11.490372840975784 1102 38-39 11.415387441129955 1102 40-41 10.911319561213649 1102 42-43 10.691201257647311 1102 44-45 10.665555228976068 1102 46-47 10.512881539087413 1102 48-49 10.345250060405018 1102 50-51 12.257576363764866 1102 52-53 11.318566921764958 1102 54-55 10.9518146302227 1102 56-57 10.814206860428367 1102 58-59 10.846187769051163 1102 60-61 10.802527884804686 1102 62-63 10.727345487966712 1102 64-65 10.461571852503067 1102 66-67 10.240236535456816 1102 68-69 9.789382211460218 1102 70-71 9.592029658089967 1102 72-73 9.707134925993739 1102 74-75 9.267151385785294 1102 76-77 9.122547942656983 1102 78-79 8.70602141177152 1102 80-81 8.376046092352487 1102 82-83 8.452768087194828 1102 84-85 8.241488255875701 1102 86-87 7.8728358772015525 1102 88-89 7.735652006403605 1102 90-91 7.706514196822267 1102 92-93 7.438345638552491 1102 94-95 7.201750148628756 1102 96-97 7.081748473355033 1102 98-99 6.414247629607138 1102 100-101 5.779416696828328 1103 1 7.935370100874113 1103 2 9.401904457532162 1103 3 8.734670390292692 1103 4 10.977287911674814 1103 5 11.582880092127734 1103 6 10.668568611766293 1103 7 10.392332293763513 1103 8 9.770531585901384 1103 9 9.627579417562128 1103 10-11 8.93973740659019 1103 12-13 8.694034879686985 1103 14-15 9.588919801052768 1103 16-17 10.643822713181347 1103 18-19 11.572866571592773 1103 20-21 11.909244634665189 1103 22-23 12.280530082233408 1103 24-25 11.945247269758223 1103 26-27 12.062586284308555 1103 28-29 11.704240624620379 1103 30-31 11.523484081634464 1103 32-33 11.665192144191234 1103 34-35 11.415711181167737 1103 36-37 11.688109289677616 1103 38-39 11.503312477601728 1103 40-41 11.097820054859302 1103 42-43 10.712672479303667 1103 44-45 10.722181681069742 1103 46-47 10.739582945882951 1103 48-49 10.478684592147758 1103 50-51 12.48385552384854 1103 52-53 11.462989482443025 1103 54-55 10.996042884911073 1103 56-57 10.760019005246221 1103 58-59 10.706770069175114 1103 60-61 10.64172517788646 1103 62-63 10.522582627015911 1103 64-65 10.415633175523851 1103 66-67 10.388263087666619 1103 68-69 10.200835074289774 1103 70-71 10.086791056386986 1103 72-73 10.002221701712173 1103 74-75 9.705143376494924 1103 76-77 9.19850648524858 1103 78-79 8.815372907650243 1103 80-81 8.560706605365693 1103 82-83 8.568583630160914 1103 84-85 8.30003901185335 1103 86-87 8.137474866507787 1103 88-89 8.032175429669131 1103 90-91 7.753537412668356 1103 92-93 7.322121088413098 1103 94-95 6.730616755864604 1103 96-97 6.413824786055281 1103 98-99 6.1246752793116475 1103 100-101 5.792335649416909 1104 1 7.8190620213885005 1104 2 9.461573825119178 1104 3 8.707694827075638 1104 4 10.667727041561449 1104 5 11.280286396376471 1104 6 10.602026323209078 1104 7 10.172600664386035 1104 8 9.63153450260442 1104 9 9.543304762571974 1104 10-11 8.639679763518998 1104 12-13 8.587340797708958 1104 14-15 9.383942009697648 1104 16-17 10.400875048968299 1104 18-19 11.485053626086387 1104 20-21 11.779002661053706 1104 22-23 11.721343195031995 1104 24-25 11.46577859825221 1104 26-27 11.702348456634066 1104 28-29 11.475939058619968 1104 30-31 11.31171792460286 1104 32-33 11.185970925722177 1104 34-35 10.950219439098362 1104 36-37 11.217794107547338 1104 38-39 10.807803666879511 1104 40-41 10.705436971494446 1104 42-43 10.398938547840128 1104 44-45 10.469453939417377 1104 46-47 10.533416076768482 1104 48-49 10.520893291584297 1104 50-51 12.110247504006228 1104 52-53 11.283301603611065 1104 54-55 10.949324872875732 1104 56-57 10.768223293260338 1104 58-59 10.586751709462952 1104 60-61 10.353308283314487 1104 62-63 10.367523068570325 1104 64-65 9.965208489742231 1104 66-67 9.97037885507713 1104 68-69 9.762889719436362 1104 70-71 9.4459266345876 1104 72-73 9.439374388570283 1104 74-75 9.209136416897202 1104 76-77 8.795094845833567 1104 78-79 8.64106292733738 1104 80-81 8.426583119481274 1104 82-83 8.311018810497048 1104 84-85 8.06937050376029 1104 86-87 7.823646681652335 1104 88-89 7.426058839132537 1104 90-91 7.094482237763154 1104 92-93 6.778210675807507 1104 94-95 6.552726358921445 1104 96-97 6.024411290666729 1104 98-99 5.8310849039467545 1104 100-101 5.1338917577640615 1105 1 7.972419386387088 1105 2 9.16749574576756 1105 3 8.671576058816285 1105 4 10.653190240128971 1105 5 10.76074358702768 1105 6 10.475245152725616 1105 7 10.049814169239493 1105 8 9.568799487054523 1105 9 9.43403018901623 1105 10-11 8.688928773979839 1105 12-13 8.395604922202413 1105 14-15 9.349056042305321 1105 16-17 10.34930474456721 1105 18-19 10.806535460974704 1105 20-21 11.286937528172732 1105 22-23 11.716611077418197 1105 24-25 11.605910006696273 1105 26-27 11.80295719991933 1105 28-29 11.341470405806856 1105 30-31 11.200114988970029 1105 32-33 11.128157100446526 1105 34-35 11.063736072780387 1105 36-37 11.288223673563731 1105 38-39 11.0478847148486 1105 40-41 10.810720698287401 1105 42-43 10.479950292727509 1105 44-45 10.402266292164526 1105 46-47 10.531663473338085 1105 48-49 10.420785207991798 1105 50-51 12.110797051645473 1105 52-53 10.936474608040427 1105 54-55 10.630677066354199 1105 56-57 10.548648677295777 1105 58-59 10.511147089257506 1105 60-61 10.412305432499295 1105 62-63 10.423000285609245 1105 64-65 10.205070130963605 1105 66-67 9.806861337877997 1105 68-69 9.616513612094924 1105 70-71 9.492749212572711 1105 72-73 9.4265527883441 1105 74-75 8.994853773885232 1105 76-77 8.6704980241391 1105 78-79 8.536572304398916 1105 80-81 8.246385682858902 1105 82-83 8.174466529844898 1105 84-85 7.65611215157859 1105 86-87 7.533958798794924 1105 88-89 7.284050545851976 1105 90-91 7.045928260758158 1105 92-93 6.601349694278909 1105 94-95 6.2739390155984776 1105 96-97 5.913164117921463 1105 98-99 5.4817613835433985 1105 100-101 4.804127833534489 1106 1 7.893907700400849 1106 2 9.315740491785846 1106 3 8.897895444359587 1106 4 10.957078893413296 1106 5 11.563851211191281 1106 6 10.603569533085622 1106 7 9.979699429818137 1106 8 9.365330798900718 1106 9 9.152872663806544 1106 10-11 8.7280285289511 1106 12-13 8.395018266844762 1106 14-15 8.998525343030984 1106 16-17 10.309825666252255 1106 18-19 11.612368440901196 1106 20-21 11.620244945004327 1106 22-23 11.635887948118413 1106 24-25 11.368749277264552 1106 26-27 11.824262036880977 1106 28-29 11.620614984545892 1106 30-31 11.43922564065224 1106 32-33 11.45854037016662 1106 34-35 11.382704006999596 1106 36-37 11.451204601374503 1106 38-39 11.205180210089392 1106 40-41 11.0626900579142 1106 42-43 10.990566634259881 1106 44-45 10.500318136948241 1106 46-47 10.331062681706758 1106 48-49 10.515106254547263 1106 50-51 12.275949664500057 1106 52-53 11.448617961635755 1106 54-55 10.838985366702897 1106 56-57 10.736587490791205 1106 58-59 10.47351868477159 1106 60-61 10.367775875907075 1106 62-63 10.41922059943452 1106 64-65 10.442639045297788 1106 66-67 10.093835645200585 1106 68-69 9.86512737375735 1106 70-71 9.554530029649328 1106 72-73 9.431272536718428 1106 74-75 9.322755244057692 1106 76-77 9.127077870524925 1106 78-79 8.753524347090465 1106 80-81 8.544445773802261 1106 82-83 8.358279612966188 1106 84-85 8.215782540797324 1106 86-87 8.001501619923943 1106 88-89 7.716568098391797 1106 90-91 7.020215262454512 1106 92-93 6.757377342474179 1106 94-95 6.4894547539831695 1106 96-97 5.9736782659753676 1106 98-99 5.59497379283564 1106 100-101 5.247896387393691 1107 1 7.310335088881082 1107 2 8.43304057351682 1107 3 8.397986854003843 1107 4 10.226777671750291 1107 5 11.134758209943808 1107 6 10.124916373538152 1107 7 9.737783806296822 1107 8 8.349598930272506 1107 9 5.576456889728167 1107 10-11 7.67084104239369 1107 12-13 7.5095814362859805 1107 14-15 8.02747128229197 1107 16-17 9.340369930028228 1107 18-19 10.741510104341646 1107 20-21 10.922031196993832 1107 22-23 10.440524702323255 1107 24-25 10.726303477806564 1107 26-27 10.71633614859432 1107 28-29 10.730574737417239 1107 30-31 10.576382451208445 1107 32-33 11.014876268347034 1107 34-35 11.086409054962772 1107 36-37 11.262305899391446 1107 38-39 10.848701417126858 1107 40-41 10.363751081917297 1107 42-43 10.387424293312659 1107 44-45 9.892588510544837 1107 46-47 10.041315617569445 1107 48-49 10.299204068243654 1107 50-51 11.929091064465641 1107 52-53 10.760033816369699 1107 54-55 10.599457820137747 1107 56-57 10.05051643861243 1107 58-59 10.167758801775939 1107 60-61 10.030308272560408 1107 62-63 10.153456468578927 1107 64-65 10.247287225708163 1107 66-67 10.32578291258993 1107 68-69 10.13968094518592 1107 70-71 9.75696340814978 1107 72-73 9.425420975226626 1107 74-75 9.32294075186515 1107 76-77 8.920751516586783 1107 78-79 8.674750069764361 1107 80-81 8.430524488260042 1107 82-83 8.270463846415664 1107 84-85 8.057668052906415 1107 86-87 7.675664549549268 1107 88-89 7.549386580346457 1107 90-91 7.149794482353853 1107 92-93 6.636209154105785 1107 94-95 6.68106281093676 1107 96-97 6.1712326547137 1107 98-99 5.901981820753223 1107 100-101 5.338095525992221 1108 1 6.841929912562563 1108 2 8.322198977823298 1108 3 7.9891747815159135 1108 4 9.282025177493264 1108 5 11.166782771001 1108 6 9.68482609038021 1108 7 9.11029828148315 1108 8 6.378242830548196 1108 9 7.348137219204002 1108 10-11 6.300324947410687 1108 12-13 5.205338190959797 1108 14-15 7.375169607104915 1108 16-17 8.871975345034645 1108 18-19 8.566561246920255 1108 20-21 8.619413156677389 1108 22-23 9.52573191145505 1108 24-25 9.092370089578615 1108 26-27 7.719312761596765 1108 28-29 9.718872675444015 1108 30-31 9.969736597622017 1108 32-33 10.448784597950866 1108 34-35 10.13601544076553 1108 36-37 9.486823960304633 1108 38-39 9.315395756446765 1108 40-41 9.795157154667532 1108 42-43 9.099403352235132 1108 44-45 8.548587684872185 1108 46-47 9.346444477938377 1108 48-49 9.958919896235127 1108 50-51 11.435478985487709 1108 52-53 10.683702137536528 1108 54-55 10.017293701473445 1108 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2313 66-67 -16.4194745399846 2313 68-69 -15.987303181798207 2313 70-71 -15.654960711091412 2313 72-73 -15.278218204022313 2313 74-75 -14.841596607794159 2313 76-77 -14.435036327005939 2313 78-79 -14.082702504761386 2313 80-81 -13.649034164469343 2313 82-83 -13.216373164811591 2313 84-85 -12.849803878955761 2313 86-87 -12.372148071434083 2313 88-89 -11.969002889262526 2313 90-91 -11.502354181989931 2313 92-93 -10.96735259579743 2313 94-95 -10.390946480584729 2313 96-97 -9.724431301925867 2313 98-99 -9.016330219510037 2313 100-101 -7.9725897237174195 2314 1 -19.509641682315202 2314 2 -18.127160742782056 2314 3 -18.850561345763868 2314 4 -20.33535937819164 2314 5 -19.619790764117358 2314 6 -20.689640343457587 2314 7 -20.923849952897914 2314 8 -21.45295623813632 2314 9 -22.860244620144076 2314 10-11 -23.359162829073597 2314 12-13 -23.3179446961182 2314 14-15 -23.30895922487025 2314 16-17 -22.06748914856256 2314 18-19 -20.95128896650621 2314 20-21 -20.60352048709444 2314 22-23 -20.239304953116157 2314 24-25 -20.082639611624334 2314 26-27 -19.81211913595853 2314 28-29 -19.6600563117504 2314 30-31 -19.702556766755166 2314 32-33 -19.598751296500044 2314 34-35 -19.302866980654727 2314 36-37 -18.8348679294897 2314 38-39 -18.763183987441476 2314 40-41 -18.675157164308022 2314 42-43 -18.574248180554935 2314 44-45 -18.19239019638509 2314 46-47 -17.84486324421917 2314 48-49 -17.632077387428048 2314 50-51 -15.231187681178955 2314 52-53 -17.205510124784 2314 54-55 -18.027719880210686 2314 56-57 -18.02768720056682 2314 58-59 -17.77339489547532 2314 60-61 -17.501244185821317 2314 62-63 -17.0842516227877 2314 64-65 -16.76916651025777 2314 66-67 -16.4194745399846 2314 68-69 -15.987303181798207 2314 70-71 -15.654960711091412 2314 72-73 -15.278218204022313 2314 74-75 -14.841596607794159 2314 76-77 -14.435036327005939 2314 78-79 -14.082702504761386 2314 80-81 -13.649034164469343 2314 82-83 -13.216373164811591 2314 84-85 -12.849803878955761 2314 86-87 -12.372148071434083 2314 88-89 -11.969002889262526 2314 90-91 -11.502354181989931 2314 92-93 -10.96735259579743 2314 94-95 -10.390946480584729 2314 96-97 -9.724431301925867 2314 98-99 -9.016330219510037 2314 100-101 -7.9725897237174195 2315 1 -19.509641682315202 2315 2 -18.127160742782056 2315 3 -18.850561345763868 2315 4 -20.33535937819164 2315 5 -19.619790764117358 2315 6 -20.689640343457587 2315 7 -20.923849952897914 2315 8 -21.45295623813632 2315 9 -22.860244620144076 2315 10-11 -23.359162829073597 2315 12-13 -23.3179446961182 2315 14-15 -23.30895922487025 2315 16-17 -22.06748914856256 2315 18-19 -20.95128896650621 2315 20-21 -20.60352048709444 2315 22-23 -20.239304953116157 2315 24-25 -20.082639611624334 2315 26-27 -19.81211913595853 2315 28-29 -19.6600563117504 2315 30-31 -19.702556766755166 2315 32-33 -19.598751296500044 2315 34-35 -19.302866980654727 2315 36-37 -18.8348679294897 2315 38-39 -18.763183987441476 2315 40-41 -18.675157164308022 2315 42-43 -18.574248180554935 2315 44-45 -18.19239019638509 2315 46-47 -17.84486324421917 2315 48-49 -17.632077387428048 2315 50-51 -15.231187681178955 2315 52-53 -17.205510124784 2315 54-55 -18.027719880210686 2315 56-57 -18.02768720056682 2315 58-59 -17.77339489547532 2315 60-61 -17.501244185821317 2315 62-63 -17.0842516227877 2315 64-65 -16.76916651025777 2315 66-67 -16.4194745399846 2315 68-69 -15.987303181798207 2315 70-71 -15.654960711091412 2315 72-73 -15.278218204022313 2315 74-75 -14.841596607794159 2315 76-77 -14.435036327005939 2315 78-79 -14.082702504761386 2315 80-81 -13.649034164469343 2315 82-83 -13.216373164811591 2315 84-85 -12.849803878955761 2315 86-87 -12.372148071434083 2315 88-89 -11.969002889262526 2315 90-91 -11.502354181989931 2315 92-93 -10.96735259579743 2315 94-95 -10.390946480584729 2315 96-97 -9.724431301925867 2315 98-99 -9.016330219510037 2315 100-101 -7.9725897237174195 2316 1 -19.509641682315202 2316 2 -18.127160742782056 2316 3 -18.850561345763868 2316 4 -20.33535937819164 2316 5 -19.619790764117358 2316 6 -20.689640343457587 2316 7 -20.923849952897914 2316 8 -21.45295623813632 2316 9 -22.860244620144076 2316 10-11 -23.359162829073597 2316 12-13 -23.3179446961182 2316 14-15 -23.30895922487025 2316 16-17 -22.06748914856256 2316 18-19 -20.95128896650621 2316 20-21 -20.60352048709444 2316 22-23 -20.239304953116157 2316 24-25 -20.082639611624334 2316 26-27 -19.81211913595853 2316 28-29 -19.6600563117504 2316 30-31 -19.702556766755166 2316 32-33 -19.598751296500044 2316 34-35 -19.302866980654727 2316 36-37 -18.8348679294897 2316 38-39 -18.763183987441476 2316 40-41 -18.675157164308022 2316 42-43 -18.574248180554935 2316 44-45 -18.19239019638509 2316 46-47 -17.84486324421917 2316 48-49 -17.632077387428048 2316 50-51 -15.231187681178955 2316 52-53 -17.205510124784 2316 54-55 -18.027719880210686 2316 56-57 -18.02768720056682 2316 58-59 -17.77339489547532 2316 60-61 -17.501244185821317 2316 62-63 -17.0842516227877 2316 64-65 -16.76916651025777 2316 66-67 -16.4194745399846 2316 68-69 -15.987303181798207 2316 70-71 -15.654960711091412 2316 72-73 -15.278218204022313 2316 74-75 -14.841596607794159 2316 76-77 -14.435036327005939 2316 78-79 -14.082702504761386 2316 80-81 -13.649034164469343 2316 82-83 -13.216373164811591 2316 84-85 -12.849803878955761 2316 86-87 -12.372148071434083 2316 88-89 -11.969002889262526 2316 90-91 -11.502354181989931 2316 92-93 -10.96735259579743 2316 94-95 -10.390946480584729 2316 96-97 -9.724431301925867 2316 98-99 -9.016330219510037 2316 100-101 -7.9725897237174195 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 579722.0 3 21398.0 4 92870.0 5 45227.0 6 23172.0 7 23869.0 8 23295.0 9 21697.0 10 22534.0 11 24756.0 12 25947.0 13 27733.0 14 28415.0 15 29583.0 16 31968.0 17 32696.0 18 31456.0 19 32629.0 20 34267.0 21 36558.0 22 39373.0 23 42786.0 24 46282.0 25 50884.0 26 55345.0 27 60067.0 28 66390.0 29 72482.0 30 80531.0 31 88694.0 32 98770.0 33 109567.0 34 119655.0 35 123609.0 36 107254.0 37 56757.0 38 10208.0 39 105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17554201166017 20.525618182838354 14.321716566993054 22.97712323850842 2 14.668407249975287 20.859262791252114 41.88849412407762 22.58383583469498 3 18.246346265026347 26.367540040400065 30.763253162505706 24.622860532067886 4 12.008930728072018 17.547623468305055 40.25980828967594 30.183637513946987 5 12.510580244652376 38.30039436701994 36.00873315147381 13.180292236853875 6 29.122483335787454 38.99986154363464 18.515297230652923 13.362357889924992 7 25.66325563254007 33.50536574267187 23.368418771702682 17.46295985308537 8 24.095318926279294 36.9534138043625 21.111079066442677 17.840188202915538 9 25.090843244285278 17.09967911255766 21.74927170996791 36.06020593318915 10-11 23.506828186096605 27.5081071905219 29.48849091136138 19.496573712020105 12-13 24.110482776133686 25.07876976569291 29.765351069960055 21.045396388213348 14-15 21.547790544371207 26.808280636808597 27.280515413460794 24.363413405359402 16-17 20.934110889793594 30.03658202898799 27.878116607256864 21.151190473961552 18-19 20.720327808994462 28.870981556236234 29.99988166960802 20.408808965161278 20-21 21.446248452716972 27.991107125747337 30.170533702874707 20.39211071866098 22-23 21.150824438375494 27.843341145602167 29.767655131945876 21.23817928407646 24-25 21.038907115246918 28.11280224723227 29.948691586013265 20.899599051507547 26-27 20.965769889395165 28.263292055304724 29.78315782680744 20.98778022849267 28-29 20.89226307919627 28.421648690974404 29.88401696999726 20.80207125983207 30-31 21.246702361258187 28.0485208291692 29.7950608778488 20.909715931723817 32-33 21.009289016807497 28.25100204464658 29.705777874742324 21.0339310638036 34-35 21.19810730454484 28.247402723987364 29.759459728419195 20.795030243048597 36-37 21.22771704207431 28.09612364834099 29.71009990538564 20.96605940419906 38-39 21.13681516268793 28.247734825719107 29.704640822691296 20.91080918890167 40-41 21.283608263474036 28.20908894734398 29.600019667133758 20.907283122048224 42-43 21.114636002414176 28.26528850629163 29.425086698975356 21.194988792318835 44-45 21.16357957631722 28.434464964693102 29.357550244432375 21.044405214557305 46-47 21.259800970579075 28.45505667385173 29.098534826176923 21.186607529392273 48-49 21.241393158209952 28.55721201741675 28.995060519912386 21.20633430446091 50-51 21.56189214319858 28.720180752276885 28.537724785607043 21.18020231891749 52-53 21.132088233374734 28.854608965005045 28.77421090079386 21.23909190082636 54-55 20.962447595154437 28.76723750125332 28.881792255101345 21.388522648490895 56-57 20.981721168460858 28.90918890440537 28.88174420437374 21.22734572276003 58-59 21.057012454383596 28.820506886535096 28.913022357632606 21.2094583014487 60-61 21.12244324727441 28.9017778707724 28.87292963717715 21.10284924477604 62-63 20.96179387872563 28.765966593805953 28.882025747968353 21.390213779500062 64-65 20.869501449913034 28.70713563395767 28.95822007963495 21.46514283649434 66-67 20.803847179290525 28.78479740561957 28.710909870310395 21.70044554477951 68-69 20.876650067241894 28.838387873978 28.72126644988549 21.56369560889462 70-71 20.941667295408344 28.69187371749981 28.70231437154701 21.66414461554483 72-73 20.919732193145933 28.880086214120322 28.59687792025406 21.603303672479683 74-75 20.801820512820512 28.99574358974359 28.4385641025641 21.763871794871793 76-77 20.865776258699377 28.904889949572137 28.459932036943346 21.769401754785136 78-79 20.7207254196799 28.946581926576552 28.455197198048232 21.87749545569531 80-81 20.82115550797878 29.069405877111198 28.234845624846276 21.87459299006374 82-83 20.949895144774448 28.930134881767156 28.420105605622048 21.699864367836348 84-85 20.948750925761452 28.993884640290425 28.24515217191798 21.812212262030144 86-87 20.838890807618714 29.05883419606298 28.360361219669294 21.741913776649017 88-89 20.89925666627244 28.961558702602986 28.245726205496425 21.89345842562815 90-91 20.86103686397561 28.89114722410295 28.406201749365827 21.841614162555608 92-93 20.861494869186178 28.774850435827837 28.267648179500576 22.09600651548541 94-95 20.708068775570897 28.865468123264698 28.300103299501515 22.126359801662886 96-97 20.708167272188792 28.76603697472126 28.230386061842406 22.295409691247546 98-99 20.585649370217578 28.742673110012777 27.949150046545885 22.72252747322376 100-101 20.706126870155607 28.864712235782058 28.00149979894 22.427661095122343 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 142810.0 1 129542.5 2 86418.5 3 44102.0 4 27318.5 5 21507.0 6 19919.0 7 20328.0 8 21104.5 9 20888.5 10 19035.5 11 16156.5 12 12965.5 13 9840.0 14 7545.0 15 6253.5 16 5813.5 17 6280.5 18 7249.5 19 8281.0 20 9519.0 21 10939.0 22 12450.0 23 13961.0 24 15967.5 25 18411.0 26 20930.5 27 23816.5 28 27105.0 29 31229.0 30 35742.5 31 40165.0 32 45125.5 33 49475.5 34 53596.0 35 57173.5 36 60598.5 37 64701.5 38 67270.5 39 68472.0 40 69053.5 41 69412.5 42 69811.0 43 70546.0 44 71122.0 45 71340.5 46 70927.0 47 69639.0 48 67953.5 49 65972.5 50 62828.5 51 58447.0 52 53672.0 53 48495.0 54 43091.0 55 37404.5 56 31476.5 57 25709.5 58 20366.5 59 15516.0 60 11523.0 61 8529.5 62 6101.0 63 4115.5 64 2632.0 65 1617.0 66 1041.5 67 624.5 68 358.5 69 231.5 70 141.0 71 85.0 72 53.5 73 34.5 74 19.5 75 13.0 76 8.5 77 8.0 78 7.0 79 4.5 80 5.0 81 3.5 82 1.5 83 2.0 84 1.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.88851920013264 2 28.89420152810505 3 22.689287924877334 4 28.961059741969468 5 32.05051288974266 6 24.745519114544205 7 23.674547280582463 8 19.714986369938035 9 11.633122477053409 10-11 15.237181270934538 12-13 15.331948757747924 14-15 19.44831843529452 16-17 22.707418615526404 18-19 26.621683809851437 20-21 28.667971028934268 22-23 28.26787609605917 24-25 26.808345162041235 26-27 28.240173558465383 28-29 25.22927984565965 30-31 27.081442566230773 32-33 26.08818255228027 34-35 24.173916531013816 36-37 27.129198433276784 38-39 27.242055263668203 40-41 24.315633616988023 42-43 25.155909468107147 44-45 23.88004222362894 46-47 27.127730612255025 48-49 22.5749012528576 50-51 28.163371374016922 52-53 21.75238396874823 54-55 22.88576920643807 56-57 24.973341475949855 58-59 24.246956131998044 60-61 23.822135650643713 62-63 23.635970463306336 64-65 22.191551883751885 66-67 22.691996158030157 68-69 23.675477589680764 70-71 22.18208340448475 72-73 22.038526373849464 74-75 19.37321148075852 76-77 21.586458172682736 78-79 20.727948263236957 80-81 21.745975172737726 82-83 22.58408030262748 84-85 19.579099220979835 86-87 21.106025880785644 88-89 19.940617336578804 90-91 21.069371702312665 92-93 21.056140639581304 94-95 20.12732003583964 96-97 19.027343231546492 98-99 18.431139140749977 100-101 20.106749867999476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2418551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.8155246078747 #Duplication Level Percentage of deduplicated Percentage of total 1 87.80309573400139 66.5683776526876 2 8.090164734466695 12.267201670154398 3 1.9958845355043737 4.539570993480253 4 0.7960598049902438 2.41414766938311 5 0.4130897646112752 1.5659308607073652 6 0.223504642293558 1.0167073024668887 7 0.14413307805794712 0.764926744841771 8 0.09991149464992477 0.605987390499273 9 0.07847507642745817 0.5354666179191723 >10 0.3420539971428214 4.244158658088928 >50 0.010434048064670779 0.536111994657309 >100 0.00297353697716923 0.3534932943634394 >500 5.488820312245854E-5 0.03944986262958116 >1k 1.0977640624491707E-4 0.1716568389736522 >5k 0.0 0.0 >10k+ 5.488820312245854E-5 4.376812449147107 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 105177 4.348760890301672 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.134707103550845E-5 0.0 12-13 0.0 0.0 0.0 1.0336767758877113E-4 0.0 14-15 0.0 0.0 0.0 1.2404121310652535E-4 0.0 16-17 0.0 0.0 0.0 1.447147486242796E-4 0.0 18-19 0.0 0.0 0.0 1.653882841420338E-4 2.0673535517754226E-5 20-21 0.0 0.0 0.0 1.8606181965978802E-4 4.134707103550845E-5 22-23 0.0 0.0 0.0 2.0673535517754226E-4 4.134707103550845E-5 24-25 0.0 0.0 0.0 2.0673535517754226E-4 4.134707103550845E-5 26-27 0.0 0.0 0.0 2.480824262130507E-4 4.134707103550845E-5 28-29 0.0 0.0 0.0 4.5481778139059296E-4 4.134707103550845E-5 30-31 0.0 0.0 0.0 0.0012404121310652535 4.134707103550845E-5 32-33 0.0 0.0 0.0 0.003349112753876185 4.134707103550845E-5 34-35 0.0 0.0 0.0 0.0062847547973972845 4.134707103550845E-5 36-37 0.0 0.0 0.0 0.011825262316155417 4.134707103550845E-5 38-39 0.0 0.0 0.0 0.020280738342916896 4.134707103550845E-5 40-41 0.0 0.0 0.0 0.029645849932459563 4.134707103550845E-5 42-43 0.0 0.0 0.0 0.037997958281632264 4.134707103550845E-5 44-45 0.0 0.0 0.0 0.050319385450213784 4.134707103550845E-5 46-47 0.0 0.0 0.0 0.06481153384815949 4.134707103550845E-5 48-49 0.0 0.0 0.0 0.08130901519132737 4.134707103550845E-5 50-51 0.0 0.0 0.0 0.10111426221733592 4.134707103550845E-5 52-53 0.0 0.0 0.0 0.12304888340167315 4.134707103550845E-5 54-55 0.0 8.26941420710169E-5 0.0 0.14653401974984195 4.134707103550845E-5 56-57 0.0 8.26941420710169E-5 0.0 0.1745673339119167 4.134707103550845E-5 58-59 0.0 8.26941420710169E-5 0.0 0.20700411113927306 4.134707103550845E-5 60-61 0.0 8.26941420710169E-5 0.0 0.24163228313151142 4.134707103550845E-5 62-63 0.0 8.26941420710169E-5 0.0 0.2784311763531139 4.134707103550845E-5 64-65 0.0 8.26941420710169E-5 0.0 0.31649115524129945 4.134707103550845E-5 66-67 0.0 8.26941420710169E-5 0.0 0.3613940743858616 4.134707103550845E-5 68-69 0.0 8.26941420710169E-5 0.0 0.4145664077375255 4.134707103550845E-5 70-71 0.0 8.26941420710169E-5 0.0 0.4715633451599739 4.134707103550845E-5 72-73 0.0 8.26941420710169E-5 0.0 0.5298006947134876 4.134707103550845E-5 74-75 0.0 8.26941420710169E-5 0.0 0.5919866895508923 4.134707103550845E-5 76-77 0.0 8.26941420710169E-5 0.0 0.6616978513167595 4.134707103550845E-5 78-79 0.0 8.26941420710169E-5 0.0 0.7421592515518589 4.134707103550845E-5 80-81 0.0 8.26941420710169E-5 0.0 0.8320891310540899 4.134707103550845E-5 82-83 0.0 8.26941420710169E-5 0.0 0.9300196688016915 4.134707103550845E-5 84-85 0.0 8.26941420710169E-5 0.0 1.0293560069645005 4.134707103550845E-5 86-87 0.0 8.26941420710169E-5 0.0 1.1332818700122513 4.134707103550845E-5 88-89 0.0 8.26941420710169E-5 4.134707103550845E-5 1.249446466086512 4.134707103550845E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1870 0.0 54.78221 1 GTATCAA 3820 0.0 48.807796 1 ATCAACG 4465 0.0 40.489807 3 TCAACGC 4535 0.0 40.192997 4 CAACGCA 4615 0.0 39.48651 5 TATCAAC 4865 0.0 37.896816 2 AACGCAG 4985 0.0 36.541344 6 ACGCAGA 5760 0.0 31.370604 7 CGCAGAG 5940 0.0 29.840122 8 GTACATG 4030 0.0 27.70784 1 GCAGAGT 6345 0.0 26.869637 9 TACATGG 4225 0.0 25.940008 2 GTGGTAT 1035 0.0 24.744621 1 ACATGGG 4360 0.0 24.370739 3 AGAGTAC 5830 0.0 22.216581 10-11 GAGTACT 4275 0.0 21.172085 12-13 CAGAGTA 6240 0.0 21.05074 10-11 TGGTATC 1125 0.0 20.030104 2 CATGGGG 2660 0.0 19.308016 4 AGTACTT 4785 0.0 19.166367 12-13 >>END_MODULE