FastQCFastQC Report
Sat 18 Jun 2016
SRR1931043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418551
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69150.28591499621054095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55030.227532931908403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34200.14140698294143889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.055.5089761
GTATCAA66950.045.4252321
ATCAACG83800.035.7766043
TCAACGC84350.035.5425874
CAACGCA84450.035.4483955
TATCAAC84900.035.3704952
AACGCAG88400.033.9717836
ACGCAGA101450.029.227717
CGCAGAG102150.028.8416488
GCAGAGT115800.025.0731989
GTGGTAT16650.024.5443741
GCCGCGT1000.0016658923.7212128
GTACATG64500.021.9545381
TGGTATC17450.021.7834362
GAGTACT81600.021.13397212-13
ACATGGG62000.020.8444773
AGAGTAC108750.020.83104110-11
TACATGG66000.020.5898842
CAGAGTA114400.020.4450310-11
GTACTTT88750.019.43134714-15
Produced by FastQC (version 0.11.5)