##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931043_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2418551 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.365692515890714 31.0 31.0 34.0 30.0 34.0 2 31.522976774109786 31.0 31.0 34.0 30.0 34.0 3 31.58851394905462 31.0 31.0 34.0 30.0 34.0 4 35.36042407209937 37.0 35.0 37.0 33.0 37.0 5 35.241459452374585 37.0 35.0 37.0 33.0 37.0 6 35.274382884628025 37.0 35.0 37.0 32.0 37.0 7 35.15552163258083 37.0 35.0 37.0 32.0 37.0 8 35.153817306312746 37.0 35.0 37.0 32.0 37.0 9 36.66103547123877 39.0 35.0 39.0 32.0 39.0 10-11 36.60384192849355 38.5 35.0 39.0 32.0 39.0 12-13 36.490697942693785 38.0 35.0 39.0 32.0 39.0 14-15 37.558760183266756 39.0 36.0 41.0 32.0 41.0 16-17 37.51408880771999 39.0 36.0 41.0 32.0 41.0 18-19 37.54055051971201 39.0 36.0 41.0 32.0 41.0 20-21 37.52212213015149 39.0 36.0 41.0 32.0 41.0 22-23 37.42122142555604 39.0 36.0 41.0 32.0 41.0 24-25 37.30279369754865 39.0 36.0 41.0 31.5 41.0 26-27 37.057639677641696 39.0 36.0 40.0 31.0 41.0 28-29 36.873730179764664 39.0 36.0 40.0 30.5 41.0 30-31 36.63962885215156 38.5 35.0 40.0 30.0 41.0 32-33 36.47170681949647 38.0 35.0 40.0 30.0 41.0 34-35 36.55111965801011 38.0 35.0 40.0 30.0 41.0 36-37 36.62323928666379 39.0 35.0 40.0 30.0 41.0 38-39 36.614247952596415 39.0 35.0 40.0 30.0 41.0 40-41 36.49680511182109 39.0 35.0 40.0 30.0 41.0 42-43 36.35980014479745 39.0 35.0 40.0 30.0 41.0 44-45 36.19893068204888 38.5 35.0 40.0 30.0 41.0 46-47 35.95412997286392 38.0 35.0 40.0 29.0 41.0 48-49 35.73907724087687 38.0 35.0 40.0 28.0 41.0 50-51 35.4870149523413 38.0 34.5 40.0 27.5 41.0 52-53 35.27393592279013 38.0 34.0 40.0 27.0 41.0 54-55 35.12475279619905 38.0 34.0 40.0 26.5 41.0 56-57 34.96178889756718 38.0 34.0 40.0 26.0 41.0 58-59 34.76410358929789 38.0 33.5 40.0 26.0 41.0 60-61 34.45527507999625 37.5 33.0 40.0 26.0 41.0 62-63 34.199727440107736 37.0 33.0 40.0 25.5 41.0 64-65 33.88997792479877 37.0 32.5 39.5 25.0 41.0 66-67 33.56652764403149 36.0 32.0 39.0 23.5 41.0 68-69 33.224703138366735 36.0 32.0 39.0 23.0 40.0 70-71 32.84279368100983 35.5 31.5 39.0 22.5 40.0 72-73 32.41644976682319 35.0 31.0 38.0 22.0 40.0 74-75 31.896043333384327 35.0 31.0 37.0 21.0 39.5 76-77 30.514226906937253 33.5 29.5 36.0 20.0 38.5 78-79 31.24398245064917 34.0 30.0 36.0 20.5 39.0 80-81 31.203207209606084 34.0 31.0 36.0 20.0 38.5 82-83 30.925798753055034 34.0 30.5 36.0 20.0 37.0 84-85 30.588933001619566 34.0 30.0 35.0 19.5 37.0 86-87 30.23962819059842 34.0 30.0 35.0 18.0 36.5 88-89 29.892893513512842 34.0 30.0 35.0 16.5 36.0 90-91 29.556036651697646 34.0 29.0 35.0 10.0 36.0 92-93 29.241923986717666 34.0 29.0 35.0 6.5 35.0 94-95 28.858802439973356 34.0 29.0 35.0 2.0 35.0 96-97 28.438323401077753 34.0 29.0 35.0 2.0 35.0 98-99 27.944937898766657 34.0 29.0 35.0 2.0 35.0 100-101 26.804299764611123 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29683124536003547 1101 2 0.36457407687826304 1101 3 0.3051570276561719 1101 4 0.34139580773146605 1101 5 0.3904083046550042 1101 6 0.307902557431909 1101 7 0.4250113675831173 1101 8 0.3764315450562137 1101 9 0.5093415422334289 1101 10-11 0.3794013068577442 1101 12-13 0.44896106089250054 1101 14-15 0.6009956646933929 1101 16-17 0.5610391449491772 1101 18-19 0.5274428057763103 1101 20-21 0.4280435450014224 1101 22-23 0.5596326241028109 1101 24-25 0.412130008407793 1101 26-27 0.5046085238060485 1101 28-29 0.5832378855692113 1101 30-31 0.5175139988917579 1101 32-33 0.5121474891474804 1101 34-35 0.6054899887966485 1101 36-37 0.567087337944777 1101 38-39 0.4975141857322569 1101 40-41 0.4649615552520743 1101 42-43 0.5406218606834443 1101 44-45 0.5733995895848025 1101 46-47 0.6956185967164572 1101 48-49 0.7168560884549819 1101 50-51 0.7386830482221427 1101 52-53 0.6816420457049688 1101 54-55 0.6749511574134601 1101 56-57 0.6688370433405879 1101 58-59 0.6877847477661518 1101 60-61 0.7043172526597985 1101 62-63 0.560177635972849 1101 64-65 0.6080111399589754 1101 66-67 0.5945051948939124 1101 68-69 0.6161224675545256 1101 70-71 0.5979483513244404 1101 72-73 0.6019010716970357 1101 74-75 0.6549947261419113 1101 76-77 0.5113060213642306 1101 78-79 0.5492944823050401 1101 80-81 0.4772118208427045 1101 82-83 0.4568387780324237 1101 84-85 0.4945801367307503 1101 86-87 0.4246799869607223 1101 88-89 0.4157475524111547 1101 90-91 0.37703912168638354 1101 92-93 0.2638364813260665 1101 94-95 0.5179222010442643 1101 96-97 0.6924428177928377 1101 98-99 0.48069646865415194 1101 100-101 0.6949559457872034 1102 1 0.2923392566821512 1102 2 0.41560337460713725 1102 3 0.30720030570856593 1102 4 0.24230591689989467 1102 5 0.37091834234220755 1102 6 0.30869954117950726 1102 7 0.3154368723394896 1102 8 0.29848637498515274 1102 9 0.48065810513531204 1102 10-11 0.5078391868011991 1102 12-13 0.46907002189374936 1102 14-15 0.4424992914082324 1102 16-17 0.5326769744728352 1102 18-19 0.5048643918298197 1102 20-21 0.4429920561815308 1102 22-23 0.40285658690704906 1102 24-25 0.42994103949255447 1102 26-27 0.45719700575544664 1102 28-29 0.6873803661591822 1102 30-31 0.5170532002805359 1102 32-33 0.5544328619827041 1102 34-35 0.5797892972796248 1102 36-37 0.535508671899315 1102 38-39 0.5514072798162246 1102 40-41 0.5201143463705336 1102 42-43 0.6708166948884937 1102 44-45 0.5531901081108828 1102 46-47 0.5688940323849536 1102 48-49 0.6047456052865741 1102 50-51 0.5017783926882586 1102 52-53 0.38842334825882574 1102 54-55 0.5213526042700423 1102 56-57 0.3129358533696518 1102 58-59 0.42053575376866803 1102 60-61 0.29918775676198806 1102 62-63 0.18747740078685382 1102 64-65 0.21755013392455425 1102 66-67 0.1537739802555862 1102 68-69 0.1927826509166337 1102 70-71 0.21314696701393387 1102 72-73 0.07693338184895993 1102 74-75 0.14505620318654522 1102 76-77 0.24976116529997228 1102 78-79 0.31692681032727776 1102 80-81 0.2496217433302519 1102 82-83 0.021729529829826788 1102 84-85 0.18572220885657842 1102 86-87 0.19331751970965527 1102 88-89 0.23908984217178642 1102 90-91 0.1921659370382507 1102 92-93 0.2031679882715558 1102 94-95 0.27664785674090453 1102 96-97 0.2256819863130204 1102 98-99 0.4483137186054904 1102 100-101 0.6858586036761452 1103 1 0.350265705371374 1103 2 0.4069232809468595 1103 3 0.3330123622628314 1103 4 0.21623600457085246 1103 5 0.2327293977351701 1103 6 0.25271678284143206 1103 7 0.4185536488522956 1103 8 0.357636850536764 1103 9 0.4348146267428703 1103 10-11 0.33472623084094977 1103 12-13 0.35412419052007493 1103 14-15 0.5006107784101559 1103 16-17 0.46509212479203654 1103 18-19 0.5157915819331933 1103 20-21 0.5104011667493253 1103 22-23 0.4301842553990909 1103 24-25 0.42748337134095493 1103 26-27 0.42678200296253266 1103 28-29 0.5031085281707846 1103 30-31 0.5180962867826366 1103 32-33 0.6832064456988292 1103 34-35 0.6915146108990697 1103 36-37 0.46871504226090366 1103 38-39 0.5216979129057435 1103 40-41 0.5547500825330118 1103 42-43 0.656732190066144 1103 44-45 0.6466128629688441 1103 46-47 0.7430882173274398 1103 48-49 0.648722939196567 1103 50-51 0.5943109461917473 1103 52-53 0.6277507040455816 1103 54-55 0.5477319120060926 1103 56-57 0.613329319846514 1103 58-59 0.6587881851153554 1103 60-61 0.4942104152865525 1103 62-63 0.5409119262250499 1103 64-65 0.4471260967227124 1103 66-67 0.474066788412145 1103 68-69 0.4201346864641664 1103 70-71 0.43341364284319894 1103 72-73 0.3767619613473556 1103 74-75 0.4580920104642594 1103 76-77 0.4780631377390385 1103 78-79 0.7596221471096136 1103 80-81 0.5480354259520333 1103 82-83 0.47488173786963017 1103 84-85 0.486611842802251 1103 86-87 0.46161282824764527 1103 88-89 0.6237406777127603 1103 90-91 0.6346866380024672 1103 92-93 0.3323456931408053 1103 94-95 0.5196457169792872 1103 96-97 0.6450574520610708 1103 98-99 0.532006831145349 1103 100-101 0.6910587476718888 1104 1 0.2541739177209301 1104 2 0.3963993290688208 1104 3 0.3729564042361986 1104 4 0.16825042079383223 1104 5 0.3011673614254917 1104 6 0.2718396141042234 1104 7 0.3056741501469915 1104 8 0.39056250006689197 1104 9 0.5044081794471822 1104 10-11 0.32080673232960777 1104 12-13 0.30595267601657383 1104 14-15 0.43273409215333203 1104 16-17 0.4650036296459348 1104 18-19 0.5052077746053172 1104 20-21 0.48463271369296734 1104 22-23 0.4791141914886268 1104 24-25 0.46067147565369737 1104 26-27 0.5249220592599926 1104 28-29 0.47306988124945093 1104 30-31 0.539226624373434 1104 32-33 0.532504667067613 1104 34-35 0.6039895776830875 1104 36-37 0.6091136522809748 1104 38-39 0.6716918819009194 1104 40-41 0.5217594254383684 1104 42-43 0.5214188687584524 1104 44-45 0.5040033896240672 1104 46-47 0.45235196195711325 1104 48-49 0.518666138647653 1104 50-51 0.26227927947659424 1104 52-53 0.15586836985880836 1104 54-55 0.31639984185004977 1104 56-57 0.4078373777538644 1104 58-59 0.5117213749168386 1104 60-61 0.29391347164219894 1104 62-63 0.15450150755815173 1104 64-65 0.11319799721052703 1104 66-67 0.07713181173608064 1104 68-69 -0.12001150024362062 1104 70-71 0.04642239901361478 1104 72-73 0.021744741161725756 1104 74-75 0.21005617841592183 1104 76-77 0.39205570535902723 1104 78-79 0.4993773186204855 1104 80-81 0.3590119153374651 1104 82-83 0.41498764716331493 1104 84-85 0.4679831599494584 1104 86-87 0.48048305435880323 1104 88-89 0.5127817055172095 1104 90-91 0.4001404059560727 1104 92-93 0.5880248388353344 1104 94-95 0.5985161067458584 1104 96-97 0.5897008590514616 1104 98-99 0.5760019505774281 1104 100-101 0.80140613354952 1105 1 0.24393336546383892 1105 2 0.253865048605153 1105 3 0.2903770054990389 1105 4 0.14507753416674518 1105 5 0.20953780247722875 1105 6 0.24787038405992945 1105 7 0.2650588688351476 1105 8 0.29181709435583514 1105 9 0.22992415343644268 1105 10-11 0.3119426997219321 1105 12-13 0.27133559232983373 1105 14-15 0.3909502122174118 1105 16-17 0.4100724261778339 1105 18-19 0.34590227795089845 1105 20-21 0.313424769107165 1105 22-23 0.33048423201260846 1105 24-25 0.3775808150185114 1105 26-27 0.38957695747882326 1105 28-29 0.39592587974157567 1105 30-31 0.24354407795933497 1105 32-33 0.3026571032187775 1105 34-35 0.4625567485435127 1105 36-37 0.3773573969798747 1105 38-39 0.4044895093629961 1105 40-41 0.3214952538240041 1105 42-43 0.36750901106361056 1105 44-45 0.22769784584874486 1105 46-47 0.3391922691854603 1105 48-49 0.40658109718125246 1105 50-51 0.37060259961607045 1105 52-53 0.2641860943899559 1105 54-55 0.38756478860342014 1105 56-57 0.35698183869062916 1105 58-59 0.4254029317917727 1105 60-61 0.4402645459990069 1105 62-63 0.34934530173400447 1105 64-65 0.26571984953358907 1105 66-67 0.3627051554360108 1105 68-69 0.2343948940641667 1105 70-71 0.2373713552854113 1105 72-73 0.23129114348007818 1105 74-75 0.36680212601747186 1105 76-77 0.5077684680581136 1105 78-79 0.5406623058978361 1105 80-81 0.3569615666427133 1105 82-83 0.44997993111393697 1105 84-85 0.5260481985886081 1105 86-87 0.5027550480446017 1105 88-89 0.41455698588663736 1105 90-91 0.4816427973423707 1105 92-93 0.5196117533579994 1105 94-95 0.5861904541212226 1105 96-97 0.651356511204888 1105 98-99 0.6876873478067189 1105 100-101 0.6754307306657168 1106 1 0.15232206586907893 1106 2 0.2578962426490712 1106 3 0.2892372684337303 1106 4 0.17828128499136398 1106 5 0.23172291698104885 1106 6 0.257564922746198 1106 7 0.15173896496180816 1106 8 0.19727546302985388 1106 9 0.3412137350027393 1106 10-11 0.3385536459098475 1106 12-13 0.19040483651040319 1106 14-15 0.30541927733851537 1106 16-17 0.3803199876706316 1106 18-19 0.28935129312383623 1106 20-21 0.30176234332260066 1106 22-23 0.2858271544515887 1106 24-25 0.393734747258641 1106 26-27 0.4184405777785116 1106 28-29 0.5072133997679629 1106 30-31 0.32510625400306736 1106 32-33 0.4784923213886003 1106 34-35 0.4556485283003653 1106 36-37 0.32149791154024143 1106 38-39 0.33970885720956545 1106 40-41 0.3657023266729382 1106 42-43 0.4091503502399334 1106 44-45 0.46060061184629575 1106 46-47 0.33256029529044895 1106 48-49 0.4588667559316022 1106 50-51 0.353328662192645 1106 52-53 0.37461836985880836 1106 54-55 0.5312918171586887 1106 56-57 0.5698744147908954 1106 58-59 0.5146148934353576 1106 60-61 0.569376434605168 1106 62-63 0.3292700260766708 1106 64-65 0.3624263922722548 1106 66-67 0.2839219351928648 1106 68-69 0.47296689481810006 1106 70-71 0.3637449298778179 1106 72-73 0.4202786917790178 1106 74-75 0.5455114253295044 1106 76-77 0.5531282362232268 1106 78-79 0.46754861491677957 1106 80-81 0.4568128412633925 1106 82-83 0.46514196815097364 1106 84-85 0.5565248266161262 1106 86-87 0.43708027658102466 1106 88-89 0.4894406561344944 1106 90-91 0.447208307190639 1106 92-93 0.5882177400699007 1106 94-95 0.676062403042156 1106 96-97 0.7903181430020787 1106 98-99 0.7773908394663174 1106 100-101 0.8889832940433458 1107 1 0.1412856955847417 1107 2 0.17966571828033295 1107 3 0.15362702840275944 1107 4 0.04425861539959186 1107 5 0.11024353254434516 1107 6 0.1219197319688945 1107 7 0.16745873525474764 1107 8 0.16242822467651763 1107 9 0.19365840528277545 1107 10-11 0.17024832694396252 1107 12-13 0.1851528147441499 1107 14-15 0.15831293028298177 1107 16-17 0.16928267640466288 1107 18-19 0.22463595351012344 1107 20-21 0.13020794920723944 1107 22-23 0.09506732522630301 1107 24-25 0.1910829265936016 1107 26-27 0.14920220831148612 1107 28-29 0.24675573318733512 1107 30-31 0.2585479346500321 1107 32-33 0.26508037576120813 1107 34-35 0.10365754559291673 1107 36-37 0.20616044864180338 1107 38-39 0.25677611319958515 1107 40-41 0.2625263792388566 1107 42-43 0.18774947055653257 1107 44-45 0.20225410477011252 1107 46-47 0.1826531836201184 1107 48-49 0.20526346841063514 1107 50-51 0.1108390398757848 1107 52-53 0.0928031087498482 1107 54-55 -0.061954085538431514 1107 56-57 0.15734201813756954 1107 58-59 0.2986508711959246 1107 60-61 0.16187627329402687 1107 62-63 -0.056727360682913286 1107 64-65 -0.010246022447923053 1107 66-67 0.0010184960394283848 1107 68-69 -0.007689211346132652 1107 70-71 -0.01076575967352511 1107 72-73 0.04311241763610951 1107 74-75 0.098204380334451 1107 76-77 0.04258655342544415 1107 78-79 0.2378113101032575 1107 80-81 0.21864837380522673 1107 82-83 0.3651754402118854 1107 84-85 0.2969725187914598 1107 86-87 0.20624941668512875 1107 88-89 0.2579719451180189 1107 90-91 0.3950476789375337 1107 92-93 0.20016161992536752 1107 94-95 0.2674868341515442 1107 96-97 0.4629056005234773 1107 98-99 0.5195954787746153 1107 100-101 0.6649369117144417 1108 1 0.030664489588879462 1108 2 0.05554891083918534 1108 3 0.0788626403131758 1108 4 0.05877504029109559 1108 5 0.05012852427635295 1108 6 0.12939810876496693 1108 7 0.13984151745609097 1108 8 -0.012277257618613646 1108 9 0.06561199094203118 1108 10-11 0.0996242455160612 1108 12-13 0.0681270120231261 1108 14-15 0.06426309156406518 1108 16-17 0.10234212258203002 1108 18-19 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-0.8485853098173024 2316 84-85 -0.9122888819670258 2316 86-87 -0.6624747241876534 2316 88-89 -0.8543806765905799 2316 90-91 -0.784580333213885 2316 92-93 -0.6741280433827441 2316 94-95 -0.9704675470938717 2316 96-97 -0.8748394657662288 2316 98-99 -0.8727707087901138 2316 100-101 -1.332925816158717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 37.0 5 60.0 6 105.0 7 252.0 8 726.0 9 1406.0 10 2571.0 11 4162.0 12 5993.0 13 7868.0 14 9725.0 15 12450.0 16 14793.0 17 17692.0 18 15322.0 19 11960.0 20 11701.0 21 12260.0 22 13540.0 23 15576.0 24 19311.0 25 24801.0 26 31553.0 27 41078.0 28 52020.0 29 65737.0 30 81186.0 31 98727.0 32 118859.0 33 146050.0 34 179855.0 35 226167.0 36 296967.0 37 411089.0 38 394936.0 39 71990.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.18456518062669 21.721141843030285 15.279264617164529 22.815028359178495 2 14.862043599738506 22.09990121604741 40.749228108231485 22.2888270759826 3 18.124164256776705 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20.774987254723197 27.851871974159646 30.547875311706264 20.825265459410893 24-25 20.777466202694523 28.133155760344273 30.48492972608223 20.60444831087898 26-27 20.681369835032818 28.29891985920088 30.411175444421517 20.608534861344786 28-29 20.6435559539583 28.380372260777285 30.39594235628713 20.58012942897728 30-31 21.020659426994225 28.047035473016628 30.35838962945351 20.573915470535635 32-33 20.823620662951917 28.205739534250874 30.284714529686184 20.68592527311102 34-35 20.972551867275683 28.221347518449036 30.345310415883116 20.46079019839216 36-37 20.940694408696185 28.173138049623063 30.335296300545888 20.55087124113486 38-39 20.83870243499657 28.232960678225815 30.376170305665116 20.55216658111249 40-41 20.99021609356991 28.1545991596074 30.268046991694277 20.58713775512841 42-43 20.870979807313184 28.262814446569696 30.04586117427019 20.82034457184693 44-45 20.85359058623103 28.414026375175865 29.99451077119013 20.737872267402974 46-47 21.013546762381683 28.404699188269394 29.692385942132667 20.889368107216256 48-49 20.991058159181637 28.55186342107287 29.556952132371002 20.900126287374487 50-51 21.14572291035715 28.630873156026915 29.28832611920483 20.93507781441111 52-53 21.197939411751314 28.62874433679731 29.232060401976145 20.94125584947523 54-55 21.01670670447548 28.704048705384448 29.33095849645863 20.948286093681443 56-57 20.980310916822557 28.759868110929727 29.27014434360626 20.98967662864146 58-59 21.038443676443038 28.68303289884782 29.405870454971218 20.872652969737924 60-61 21.196188877069947 28.76370743912468 29.322737267365 20.717366416440377 62-63 21.042290143848906 28.701770789169288 29.341939649688193 20.913999417293617 64-65 20.92042171298831 28.577367307006046 29.383321223957417 21.11888975604823 66-67 20.900195524369668 28.658372045113612 29.305069830428103 21.136362600088624 68-69 20.925170479697456 28.75963067291607 29.21416030148338 21.1010385459031 70-71 21.05348444506886 28.565804079834507 29.185324067368267 21.195387407728365 72-73 21.04202885466733 28.776645924458382 29.067693920873705 21.11363130000058 74-75 20.997046111750254 28.868650435828076 29.02459500637418 21.109708446047488 76-77 20.904027954185597 28.783182077798802 29.01779786001403 21.29499210800157 78-79 20.895241273241773 28.81079486331823 29.04934007247325 21.24462379096675 80-81 20.93607740174898 29.009737239254925 28.87542122346034 21.178764135535754 82-83 21.080762820382947 28.828687094049286 29.032280071828133 21.058270013739634 84-85 21.0259779512609 28.891989459804652 28.867429299609558 21.21460328932489 86-87 20.9617039293362 28.937905382189584 28.877104514231867 21.223286174242347 88-89 20.96868391571064 28.83862827698999 28.822048091221617 21.370639716077758 90-91 20.885286770152806 28.81485483223043 29.02096290034099 21.27889549727577 92-93 21.01462970422159 28.782979487149795 28.95795200682852 21.244438801800094 94-95 20.776072944869593 28.79979772452495 28.94029896414448 21.483830366460975 96-97 20.78821161927121 28.688789279200645 28.950950383101286 21.57204871842686 98-99 20.69981158139729 28.769292026506783 28.828397664552043 21.70249872754389 100-101 20.69861251633726 28.727283402334702 28.921469921452967 21.652634159875063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 155.0 2 154.5 3 223.5 4 209.5 5 205.5 6 403.0 7 848.5 8 1460.5 9 1850.0 10 1711.5 11 1512.0 12 1551.0 13 1730.0 14 1994.5 15 2239.5 16 2543.0 17 2806.0 18 3129.5 19 3499.0 20 4002.0 21 4702.5 22 5626.0 23 7048.0 24 9460.5 25 12445.5 26 16059.5 27 20518.5 28 25453.5 29 31831.5 30 39501.0 31 46972.0 32 54631.0 33 61789.0 34 68462.5 35 73746.5 36 80413.0 37 87738.0 38 91291.0 39 93463.0 40 94241.5 41 94639.0 42 95571.0 43 96919.0 44 98673.5 45 100654.0 46 100268.0 47 98123.5 48 96864.0 49 94312.5 50 89054.5 51 82774.5 52 76081.0 53 68514.0 54 60821.0 55 51908.0 56 41983.0 57 32764.0 58 25135.5 59 18584.0 60 12918.0 61 9005.5 62 6357.0 63 4042.0 64 2236.5 65 1229.5 66 661.5 67 344.5 68 174.5 69 88.5 70 48.5 71 31.0 72 20.0 73 10.5 74 6.5 75 5.0 76 4.5 77 3.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00454817781390593 2 0.0058712840870422 3 0.0018606181965978802 4 0.16993646195593973 5 0.0014884945572783042 6 5.788589944971184E-4 7 0.0 8 9.923297048522028E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.307765682840676E-4 24-25 6.202060655326267E-4 26-27 0.0028942949724855916 28-29 1.8606181965978802E-4 30-31 0.006760246114305631 32-33 0.02201731532640825 34-35 0.013871942332413087 36-37 7.649208141569063E-4 38-39 2.2740889069529648E-4 40-41 0.0015918622348670753 42-43 0.01134977099924707 44-45 0.007380452179838258 46-47 0.010791585540267707 48-49 0.010626197256125671 50-51 0.18763300835913735 52-53 0.1131255863531511 54-55 0.017365769834913548 56-57 0.00640879601050381 58-59 0.018275405397694736 60-61 0.12145702116680608 62-63 0.23452058691340394 64-65 0.2406399534266592 66-67 0.2507286387593232 68-69 0.24589103144816876 70-71 0.23297007174957235 72-73 0.15831793499496186 74-75 0.2677636320259527 76-77 0.20799644084412527 78-79 0.005850610551524446 80-81 4.134707103550845E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 6.202060655326267E-5 90-91 0.0065948578301635976 92-93 0.018895611463227362 94-95 0.002770253759379066 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2418551.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.54684726397205 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6352609104407 45.03841329204688 2 14.513344065408162 17.2844776470467 3 4.412702773671053 7.882876142552882 4 1.9468950122361113 4.637258397304508 5 0.9938910098887118 2.959153808143903 6 0.5947460086109315 2.1249149841367285 7 0.38901189877451725 1.621510248413575 8 0.25520998416822355 1.2157559958004747 9 0.1912403381360713 1.0248983285129105 >10 0.9827869019039903 10.424754470823974 >50 0.0589871268971894 2.38066092237321 >100 0.02515710377861714 2.5141216015988106 >500 4.880056908845432E-4 0.18373717242899437 >1k 1.394301973937285E-4 0.19195677480854872 >5k 1.394301973937285E-4 0.5155102140079062 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6915 0.28591499621054095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5503 0.227532931908403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3420 0.14140698294143889 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.134707103550845E-5 0.0 10-11 0.0 0.0 0.0 4.134707103550845E-5 0.0 12-13 0.0 0.0 0.0 4.134707103550845E-5 0.0 14-15 0.0 0.0 0.0 4.134707103550845E-5 0.0 16-17 0.0 0.0 0.0 4.134707103550845E-5 0.0 18-19 0.0 0.0 0.0 4.134707103550845E-5 0.0 20-21 0.0 0.0 0.0 4.134707103550845E-5 0.0 22-23 0.0 0.0 0.0 4.134707103550845E-5 0.0 24-25 0.0 0.0 0.0 4.134707103550845E-5 0.0 26-27 0.0 0.0 0.0 4.134707103550845E-5 0.0 28-29 0.0 0.0 0.0 3.5145010380182185E-4 0.0 30-31 0.0 0.0 0.0 0.0020880270872931767 0.0 32-33 0.0 0.0 0.0 0.006367448939468301 0.0 34-35 0.0 0.0 0.0 0.012259406562028256 0.0 36-37 0.0 0.0 0.0 0.022348091894692317 0.0 38-39 0.0 0.0 0.0 0.038328734849916335 0.0 40-41 0.0 0.0 0.0 0.05827869662454916 0.0 42-43 0.0 0.0 0.0 0.0773190228364008 0.0 44-45 0.0 0.0 0.0 0.10194120363804608 0.0 46-47 0.0 0.0 0.0 0.13154570649947014 0.0 48-49 0.0 0.0 0.0 0.1646233633278769 0.0 50-51 0.0 0.0 0.0 0.19925153532011522 0.0 52-53 0.0 0.0 0.0 0.23563695783136268 0.0 54-55 0.0 0.0 0.0 0.2747719605664714 0.0 56-57 0.0 0.0 0.0 0.32134943608797173 0.0 58-59 0.0 0.0 0.0 0.3762996934941624 0.0 60-61 0.0 0.0 0.0 0.43517792264872646 0.0 62-63 0.0 0.0 0.0 0.4983769207265011 0.0 64-65 0.0 0.0 0.0 0.5632918222522494 0.0 66-67 0.0 0.0 0.0 0.638688206285499 0.0 68-69 0.0 0.0 0.0 0.7285560651811767 0.0 70-71 0.0 0.0 0.0 0.8266313176774027 0.0 72-73 0.0 0.0 0.0 0.9282830918182002 0.0 74-75 0.0 0.0 0.0 1.0345037173084215 0.0 76-77 0.0 0.0 0.0 1.1541207938141476 0.0 78-79 0.0 0.0 0.0 1.2941426498758968 0.0 80-81 0.0 0.0 0.0 1.4509100697070272 0.0 82-83 0.0 0.0 0.0 1.6215287583350526 0.0 84-85 0.0 0.0 0.0 1.793098429596895 0.0 86-87 0.0 0.0 0.0 1.980504029065337 0.0 88-89 0.0 0.0 0.0 2.189120675974995 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3390 0.0 55.508976 1 GTATCAA 6695 0.0 45.425232 1 ATCAACG 8380 0.0 35.776604 3 TCAACGC 8435 0.0 35.542587 4 CAACGCA 8445 0.0 35.448395 5 TATCAAC 8490 0.0 35.370495 2 AACGCAG 8840 0.0 33.971783 6 ACGCAGA 10145 0.0 29.22771 7 CGCAGAG 10215 0.0 28.841648 8 GCAGAGT 11580 0.0 25.073198 9 GTGGTAT 1665 0.0 24.544374 1 GCCGCGT 100 0.00166589 23.721212 8 GTACATG 6450 0.0 21.954538 1 TGGTATC 1745 0.0 21.783436 2 GAGTACT 8160 0.0 21.133972 12-13 ACATGGG 6200 0.0 20.844477 3 AGAGTAC 10875 0.0 20.831041 10-11 TACATGG 6600 0.0 20.589884 2 CAGAGTA 11440 0.0 20.44503 10-11 GTACTTT 8875 0.0 19.431347 14-15 >>END_MODULE