FastQCFastQC Report
Sat 18 Jun 2016
SRR1931042_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931042_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603708
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1145084.397881790123931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.050.810621
GTATCAA33350.042.5410421
TCAACGC37050.036.5593574
ATCAACG36950.036.5241283
CAACGCA38000.036.0346035
TATCAAC39500.035.0058022
AACGCAG41800.032.3635836
ACGCAGA47400.027.8226477
CGCAGAG48450.026.653768
GTGTAGC8750.025.7554321
ACATGGG36700.023.0516873
GTACATG38350.022.8378351
GCAGAGT53800.022.3634459
TACATGG38450.021.9766242
GAGTACT36850.020.5908512-13
GTATACG2301.0004242E-620.0418991
CAGAGTA53650.019.76770610-11
GTGGTAT11400.018.869861
AGAGTAC49300.018.49188210-11
TGGTATC10550.018.4460962
Produced by FastQC (version 0.11.5)