##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931042_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2603708 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.964703031215482 28.0 2.0 31.0 2.0 33.0 2 19.70301470057318 27.0 2.0 31.0 2.0 33.0 3 20.34321821033695 27.0 2.0 31.0 2.0 33.0 4 21.872595544508062 30.0 2.0 35.0 2.0 37.0 5 21.229884457089657 30.0 2.0 35.0 2.0 37.0 6 22.181495390420125 30.0 2.0 35.0 2.0 37.0 7 22.34510897535361 30.0 2.0 35.0 2.0 37.0 8 22.76896756471924 31.0 2.0 35.0 2.0 37.0 9 24.03974869685848 32.0 2.0 37.0 2.0 39.0 10-11 24.38766578279899 32.0 2.0 37.0 2.0 39.0 12-13 24.289127467442587 32.0 2.0 37.0 2.0 39.0 14-15 24.250352190030526 32.0 2.0 38.0 2.0 40.0 16-17 23.103032674938973 31.0 2.0 38.0 2.0 40.0 18-19 22.06736930562106 28.5 2.0 37.0 2.0 40.0 20-21 21.729203313121133 27.0 2.0 37.0 2.0 40.0 22-23 21.368719149766413 27.0 2.0 37.0 2.0 39.5 24-25 21.205377292691807 27.0 2.0 37.0 2.0 39.0 26-27 20.932835978535227 27.0 2.0 37.0 2.0 39.0 28-29 20.73429931466969 25.5 2.0 36.5 2.0 39.0 30-31 20.723716138676075 25.5 2.0 36.0 2.0 39.0 32-33 20.597758081935453 25.5 2.0 36.0 2.0 39.0 34-35 20.278605166170706 25.0 2.0 36.0 2.0 39.0 36-37 19.83532504412937 24.0 2.0 36.0 2.0 39.0 38-39 19.71945702052611 24.0 2.0 35.5 2.0 39.0 40-41 19.59678485452286 24.0 2.0 35.0 2.0 39.0 42-43 19.463571567933116 24.0 2.0 35.0 2.0 39.0 44-45 19.088084762192995 23.0 2.0 35.0 2.0 38.0 46-47 18.76620592631739 19.0 2.0 35.0 2.0 38.0 48-49 18.53083813545912 18.5 2.0 35.0 2.0 38.0 50-51 16.287439874210165 11.0 2.0 33.0 2.0 37.5 52-53 18.170464967653825 18.5 2.0 34.0 2.0 38.0 54-55 18.946406432672173 22.0 2.0 35.5 2.0 39.0 56-57 18.919395147228492 21.5 2.0 36.0 2.0 39.0 58-59 18.649717249399703 20.0 2.0 35.5 2.0 39.0 60-61 18.365267917907843 18.5 2.0 35.0 2.0 39.0 62-63 17.93089317235266 14.5 2.0 34.5 2.0 38.5 64-65 17.578168903732674 12.5 2.0 34.0 2.0 38.0 66-67 17.21755319720952 8.0 2.0 34.0 2.0 38.0 68-69 16.776810610099133 8.0 2.0 33.5 2.0 38.0 70-71 16.42946578494977 6.5 2.0 33.0 2.0 37.5 72-73 16.029655015078497 2.0 2.0 32.0 2.0 36.5 74-75 15.576371083086123 2.0 2.0 32.0 2.0 36.0 76-77 15.152133995056282 2.0 2.0 31.0 2.0 36.0 78-79 14.764525246302581 2.0 2.0 31.0 2.0 35.0 80-81 14.321903992306357 2.0 2.0 30.5 2.0 35.0 82-83 13.850757650243423 2.0 2.0 30.0 2.0 34.0 84-85 13.43939527781149 2.0 2.0 30.0 2.0 34.0 86-87 12.952665967151463 2.0 2.0 29.0 2.0 34.0 88-89 12.520849112112419 2.0 2.0 29.0 2.0 34.0 90-91 12.031570168390619 2.0 2.0 28.5 2.0 34.0 92-93 11.480917407021064 2.0 2.0 26.5 2.0 34.0 94-95 10.912349042212107 2.0 2.0 26.0 2.0 33.5 96-97 10.271363378689163 2.0 2.0 25.0 2.0 33.0 98-99 9.589680179190601 2.0 2.0 21.5 2.0 32.5 100-101 8.671774830357322 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.668534980190586 1101 2 9.279842186685851 1101 3 8.671389140184257 1101 4 10.599715717897855 1101 5 11.375537376895654 1101 6 10.636024125999132 1101 7 10.169117941706066 1101 8 9.617026962531927 1101 9 9.55729440631605 1101 10-11 8.997003641089222 1101 12-13 8.87287180674755 1101 14-15 9.656880407109767 1101 16-17 10.625038390869335 1101 18-19 11.582566369617538 1101 20-21 11.655900527517424 1101 22-23 11.934782100368583 1101 24-25 11.784419316163184 1101 26-27 11.909406323909081 1101 28-29 11.743473611690575 1101 30-31 11.218331127385866 1101 32-33 11.295231891879808 1101 34-35 11.021240603439932 1101 36-37 11.668601473083292 1101 38-39 11.354998340374962 1101 40-41 10.909358016604376 1101 42-43 10.589660615688612 1101 44-45 10.516789909419352 1101 46-47 10.178453625084074 1101 48-49 10.377805913741927 1101 50-51 12.001406574202111 1101 52-53 11.31552333925428 1101 54-55 11.111411455140306 1101 56-57 10.92806068170206 1101 58-59 10.745403177806448 1101 60-61 10.49465904795133 1101 62-63 10.647233907569198 1101 64-65 10.274287774679458 1101 66-67 10.109866683702531 1101 68-69 10.344066239419785 1101 70-71 10.016597450121655 1101 72-73 9.767861524755288 1101 74-75 9.382620597495956 1101 76-77 9.124100576757014 1101 78-79 8.85405547093107 1101 80-81 8.55824439295345 1101 82-83 8.39744453131169 1101 84-85 7.926563070160174 1101 86-87 7.883473011092557 1101 88-89 7.660894221999888 1101 90-91 7.3472475214109 1101 92-93 7.149294010354595 1101 94-95 6.817084588213005 1101 96-97 6.698408808031973 1101 98-99 6.468646774641051 1101 100-101 5.7205686282645605 1102 1 7.8514509708640965 1102 2 8.908731086656594 1102 3 8.359176437275494 1102 4 10.153948654057505 1102 5 11.141536528025558 1102 6 10.246254506683012 1102 7 9.852348380015595 1102 8 9.282622844957878 1102 9 9.096610611534278 1102 10-11 8.3782959183661 1102 12-13 8.641183463972471 1102 14-15 9.048687009900618 1102 16-17 9.984922879153594 1102 18-19 11.110310486260708 1102 20-21 11.154759512020554 1102 22-23 11.186108875460118 1102 24-25 11.009679628937459 1102 26-27 11.048663684477177 1102 28-29 10.802611238356175 1102 30-31 10.999522670600669 1102 32-33 10.973918215540902 1102 34-35 10.799876393842158 1102 36-37 10.680375251419594 1102 38-39 10.952384019958636 1102 40-41 10.499134617489151 1102 42-43 9.780032755461832 1102 44-45 10.03055606676396 1102 46-47 9.900752727675457 1102 48-49 9.588570606065307 1102 50-51 11.422534103081428 1102 52-53 10.677342857947156 1102 54-55 10.310739673089365 1102 56-57 10.176178151581578 1102 58-59 10.285531405950813 1102 60-61 10.164134549294452 1102 62-63 10.038219344903528 1102 64-65 9.751791193431732 1102 66-67 9.704925450779673 1102 68-69 9.418165340016433 1102 70-71 8.899429689675902 1102 72-73 9.048038662666757 1102 74-75 8.717138304083894 1102 76-77 8.436776085635797 1102 78-79 7.991594589879272 1102 80-81 7.784911270175362 1102 82-83 7.56444614704615 1102 84-85 7.387818965707041 1102 86-87 7.175981344647527 1102 88-89 7.013623987640818 1102 90-91 6.745962552617403 1102 92-93 6.792926902851736 1102 94-95 6.38899391425891 1102 96-97 6.252487013790992 1102 98-99 5.970226908642843 1102 100-101 5.362210388462083 1103 1 7.829781436604478 1103 2 9.182074645934613 1103 3 8.400101046633583 1103 4 10.637689672539953 1103 5 11.21254570432301 1103 6 10.356913566112528 1103 7 9.97676322873654 1103 8 9.4637402864157 1103 9 9.357099509080047 1103 10-11 8.87927685157824 1103 12-13 8.533387398526433 1103 14-15 9.397811041411686 1103 16-17 10.213087646716247 1103 18-19 11.184191989745102 1103 20-21 11.429954832974552 1103 22-23 11.496664187540798 1103 24-25 11.495659128353115 1103 26-27 11.4278307569353 1103 28-29 11.213718562080366 1103 30-31 10.88328506076645 1103 32-33 11.072668107329843 1103 34-35 10.99550087983965 1103 36-37 11.171252518441193 1103 38-39 10.937632553698844 1103 40-41 10.73591055026844 1103 42-43 10.105852589466075 1103 44-45 10.217855199993412 1103 46-47 10.30437495747245 1103 48-49 10.253078326924069 1103 50-51 11.813574363004381 1103 52-53 10.952502875044502 1103 54-55 10.701055324736338 1103 56-57 10.371031092758049 1103 58-59 10.327924439323102 1103 60-61 10.14083197495346 1103 62-63 10.16495626759755 1103 64-65 9.876052923510077 1103 66-67 9.724701021051931 1103 68-69 9.729085729143389 1103 70-71 9.450334604622011 1103 72-73 9.315290210084841 1103 74-75 8.94361457339957 1103 76-77 8.873688634871824 1103 78-79 8.48849691317062 1103 80-81 7.94367582456224 1103 82-83 7.7393341486043905 1103 84-85 7.599709331676827 1103 86-87 7.275885307336358 1103 88-89 6.843360114983584 1103 90-91 6.637832656067168 1103 92-93 6.143994134380108 1103 94-95 6.056943287716903 1103 96-97 5.469163148924956 1103 98-99 5.021966671877054 1103 100-101 4.5194767607946815 1104 1 7.864637572377514 1104 2 9.462470426919513 1104 3 8.787364404879401 1104 4 10.720631198000081 1104 5 11.574366785438915 1104 6 10.478792928481791 1104 7 10.265743078617344 1104 8 9.870324242561136 1104 9 9.509010361044059 1104 10-11 8.972587795890242 1104 12-13 8.941236173304684 1104 14-15 9.480681292469384 1104 16-17 10.361295920989818 1104 18-19 11.385339963995044 1104 20-21 11.319172935330492 1104 22-23 11.591716656776033 1104 24-25 11.42929427052609 1104 26-27 11.595126477249714 1104 28-29 11.481134482446322 1104 30-31 11.20872451913468 1104 32-33 11.186878021854252 1104 34-35 11.037293451194586 1104 36-37 11.114732588453862 1104 38-39 10.470072272466965 1104 40-41 10.51248643938641 1104 42-43 10.034656508350167 1104 44-45 10.22725677688923 1104 46-47 10.137043667070198 1104 48-49 10.001071704176393 1104 50-51 11.4995183394449 1104 52-53 10.986696668936649 1104 54-55 10.653443218557612 1104 56-57 10.362833865345724 1104 58-59 10.293926817821053 1104 60-61 10.089464405746146 1104 62-63 10.128254581172705 1104 64-65 9.777499485157737 1104 66-67 9.544023766579496 1104 68-69 9.577397290898428 1104 70-71 9.227686122994971 1104 72-73 9.087452388209103 1104 74-75 8.72084850914254 1104 76-77 8.221997847658804 1104 78-79 7.998268687433443 1104 80-81 7.689279898612449 1104 82-83 7.678466345842542 1104 84-85 7.386299744128777 1104 86-87 6.8242132447544765 1104 88-89 6.62808685617294 1104 90-91 6.3682006521155365 1104 92-93 5.833810485345467 1104 94-95 5.378455070140715 1104 96-97 5.037361208543109 1104 98-99 4.81302998931794 1104 100-101 4.129014913405124 1105 1 7.906765039175628 1105 2 9.07185955522543 1105 3 8.480340347601995 1105 4 10.642380806721878 1105 5 10.716532467971675 1105 6 10.328125294873043 1105 7 9.854529700170549 1105 8 9.624269899051551 1105 9 9.335271158760772 1105 10-11 8.974227056678245 1105 12-13 8.50977816691142 1105 14-15 9.325558218643465 1105 16-17 10.260430200740018 1105 18-19 10.680995627627283 1105 20-21 11.205622465232338 1105 22-23 11.505747395770168 1105 24-25 11.422747791819948 1105 26-27 11.626917453979324 1105 28-29 11.198111402609818 1105 30-31 11.200701117771565 1105 32-33 10.911262062165552 1105 34-35 11.23648863727188 1105 36-37 10.938447617185219 1105 38-39 11.013862228710476 1105 40-41 10.865409667492614 1105 42-43 10.572577094688725 1105 44-45 10.488865396847135 1105 46-47 10.336870226428346 1105 48-49 10.351421800726243 1105 50-51 11.65623080462488 1105 52-53 10.74201859056867 1105 54-55 10.626366675013326 1105 56-57 10.35734278134434 1105 58-59 10.291422716060111 1105 60-61 10.13636457422609 1105 62-63 10.158881369090338 1105 64-65 9.98408486202236 1105 66-67 9.734675859297798 1105 68-69 9.554761554829522 1105 70-71 9.394492161605985 1105 72-73 9.214492540190138 1105 74-75 9.036151417396693 1105 76-77 8.593091069641565 1105 78-79 8.159235933976637 1105 80-81 8.038997112674894 1105 82-83 7.930100947775534 1105 84-85 7.673202665926418 1105 86-87 7.454788286572624 1105 88-89 7.25759685748792 1105 90-91 6.8309279747122424 1105 92-93 6.543067748369197 1105 94-95 6.218725569824569 1105 96-97 5.763626770103118 1105 98-99 5.3783609819375275 1105 100-101 4.834340168415098 1106 1 7.77045272310469 1106 2 8.835827819866054 1106 3 8.284630775791435 1106 4 10.234179833352481 1106 5 10.822649386083484 1106 6 10.125668855998263 1106 7 9.328125374314194 1106 8 9.088674162843969 1106 9 8.862590410476582 1106 10-11 8.437724799102568 1106 12-13 7.966771867421901 1106 14-15 8.668520944077784 1106 16-17 9.812028347097126 1106 18-19 10.86109767108239 1106 20-21 11.029029102970313 1106 22-23 11.116872873533186 1106 24-25 10.795935524882953 1106 26-27 10.966601651983968 1106 28-29 11.05587366153022 1106 30-31 10.674107109010205 1106 32-33 10.707224285916386 1106 34-35 10.385328429564574 1106 36-37 10.530832501513558 1106 38-39 10.29740429512923 1106 40-41 10.439942765672587 1106 42-43 10.212992612741573 1106 44-45 9.69298876960831 1106 46-47 9.548747841518153 1106 48-49 9.52284795240016 1106 50-51 11.288906303334468 1106 52-53 10.595145966453725 1106 54-55 10.129414402180263 1106 56-57 9.86107023791251 1106 58-59 9.797251036868595 1106 60-61 9.68216581964241 1106 62-63 9.725074496543805 1106 64-65 9.632816042426708 1106 66-67 9.208873682922434 1106 68-69 8.827808221737548 1106 70-71 8.393243512789596 1106 72-73 8.303848187232534 1106 74-75 7.9410516237592805 1106 76-77 7.650792686924303 1106 78-79 7.458250668989491 1106 80-81 7.073590128413274 1106 82-83 6.725077906539218 1106 84-85 6.440011648646781 1106 86-87 6.289435349655443 1106 88-89 6.1168075335193475 1106 90-91 5.812144970921171 1106 92-93 5.579074011654761 1106 94-95 5.18179649338766 1106 96-97 4.651814968645651 1106 98-99 4.311125051056063 1106 100-101 3.6590451061184215 1107 1 7.401732688348886 1107 2 8.29615862064599 1107 3 8.057263424525072 1107 4 9.987815745614764 1107 5 10.859595012903377 1107 6 9.715616308532283 1107 7 9.694932288236686 1107 8 8.238935607175204 1107 9 5.8205335599110235 1107 10-11 7.7448956404309435 1107 12-13 7.590295780139318 1107 14-15 8.046083020943968 1107 16-17 9.018517907425853 1107 18-19 10.439442787943605 1107 20-21 10.806089754951962 1107 22-23 10.270125540989444 1107 24-25 10.282381149116855 1107 26-27 10.38320789251275 1107 28-29 10.381432754155469 1107 30-31 10.169788105550143 1107 32-33 10.844044233265482 1107 34-35 10.918911354741326 1107 36-37 10.827196718551143 1107 38-39 10.51709731138481 1107 40-41 10.078565670368242 1107 42-43 9.811434032143794 1107 44-45 9.21388336952866 1107 46-47 9.728854410468603 1107 48-49 9.989811448461683 1107 50-51 11.301989251785198 1107 52-53 10.316964672524097 1107 54-55 10.08754568292131 1107 56-57 9.798032932314864 1107 58-59 10.023809488166506 1107 60-61 9.610483912831413 1107 62-63 9.595258076495082 1107 64-65 9.744329290491088 1107 66-67 9.50911870617055 1107 68-69 9.30943445202578 1107 70-71 9.147367790649835 1107 72-73 8.714492540190141 1107 74-75 8.973965939323366 1107 76-77 8.805348438440989 1107 78-79 8.448775833329625 1107 80-81 7.89162112417741 1107 82-83 7.782185778832327 1107 84-85 7.387796483323974 1107 86-87 7.101804821439627 1107 88-89 7.266762925065203 1107 90-91 6.962847457099016 1107 92-93 6.437568107822827 1107 94-95 6.218366116194089 1107 96-97 5.742958186350421 1107 98-99 5.268368171010136 1107 100-101 5.011550808242561 1108 1 6.68468638638927 1108 2 7.917808146651971 1108 3 7.8229579332906845 1108 4 9.137860045567216 1108 5 10.798664786102062 1108 6 9.438414642152697 1108 7 8.708981664891489 1108 8 5.699904203201925 1108 9 7.120478275220652 1108 10-11 6.008455474246816 1108 12-13 4.930930440133313 1108 14-15 6.9588763211821245 1108 16-17 8.242019676845374 1108 18-19 7.911608596631758 1108 20-21 7.932770471425048 1108 22-23 8.978205615087429 1108 24-25 8.737709881581242 1108 26-27 7.30967706253071 1108 28-29 9.132387859067457 1108 30-31 9.499049049288686 1108 32-33 9.88388582612468 1108 34-35 9.663919100786561 1108 36-37 9.470553092329283 1108 38-39 8.984902101761971 1108 40-41 9.182444804213922 1108 42-43 8.536147603813276 1108 44-45 8.068173528544346 1108 46-47 8.96274565331176 1108 48-49 9.416093929506566 1108 50-51 10.829827053104811 1108 52-53 10.139585909477784 1108 54-55 9.486510848409218 1108 56-57 9.560324493044991 1108 58-59 9.688980752092114 1108 60-61 9.645875913260486 1108 62-63 9.563992314943317 1108 64-65 9.188382550482814 1108 66-67 8.428969313734857 1108 68-69 8.847369445068782 1108 70-71 8.885494217247551 1108 72-73 8.484676867186709 1108 74-75 8.270058260630561 1108 76-77 7.992756963422758 1108 78-79 7.751853261807634 1108 80-81 7.885300645222207 1108 82-83 7.900291824357618 1108 84-85 7.74056747371413 1108 86-87 7.414631720535782 1108 88-89 7.129972381841549 1108 90-91 6.95329176654063 1108 92-93 6.681772111424586 1108 94-95 6.488448847257791 1108 96-97 6.1235659466294035 1108 98-99 5.710408207027701 1108 100-101 4.935473891354222 1109 1 6.495497558296709 1109 2 8.175670845159289 1109 3 8.04064229895113 1109 4 9.070028919126841 1109 5 9.969030619205867 1109 6 8.637274471219555 1109 7 7.294006823882334 1109 8 8.185079617276983 1109 9 7.257643078943527 1109 10-11 5.980273396327938 1109 12-13 6.502717838438784 1109 14-15 7.901245223107281 1109 16-17 6.626399314506571 1109 18-19 5.747106514437501 1109 20-21 7.858791424917914 1109 22-23 9.277185740717943 1109 24-25 8.859420610739772 1109 26-27 9.022293402308637 1109 28-29 9.370426804116434 1109 30-31 6.813579599785495 1109 32-33 6.241177925297148 1109 34-35 7.7714552873906335 1109 36-37 6.119088773286968 1109 38-39 6.408656382284722 1109 40-41 7.545026406496323 1109 42-43 6.4215112992250525 1109 44-45 6.684669522427253 1109 46-47 6.5850439908451115 1109 48-49 7.287910515530168 1109 50-51 9.006091323226865 1109 52-53 9.27301052294607 1109 54-55 9.20771932271375 1109 56-57 8.406837177369543 1109 58-59 8.405493755014994 1109 60-61 8.208664964166335 1109 62-63 8.79226801966227 1109 64-65 8.794126621470639 1109 66-67 8.321047725922991 1109 68-69 8.078991973876239 1109 70-71 7.656081065097322 1109 72-73 7.054197592522057 1109 74-75 6.381025031852291 1109 76-77 6.0151711268657575 1109 78-79 6.931153979578843 1109 80-81 7.051051941198002 1109 82-83 6.908009355076663 1109 84-85 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56-57 -16.573821848418422 2313 58-59 -16.30994320773572 2313 60-61 -16.027366554458307 2313 62-63 -15.59956579122598 2313 64-65 -15.252255900019335 2313 66-67 -14.900298978948069 2313 68-69 -14.480105447327201 2313 70-71 -14.119885983613283 2313 72-73 -13.739856848738688 2313 74-75 -13.27513542660043 2313 76-77 -12.862156953363469 2313 78-79 -12.471245733888205 2313 80-81 -12.045294763673057 2313 82-83 -11.587871733748552 2313 84-85 -11.191283315381995 2313 86-87 -10.704629398545995 2313 88-89 -10.295242826192885 2313 90-91 -9.810696755697535 2313 92-93 -9.255638218561067 2313 94-95 -8.699858182871333 2313 96-97 -8.06796920401622 2313 98-99 -7.413695092828828 2313 100-101 -6.505523239205319 2314 1 -18.619115622219052 2314 2 -17.34402829524186 2314 3 -17.983354835719357 2314 4 -19.458266209812987 2314 5 -18.785983707441698 2314 6 -19.757424669181592 2314 7 -19.943457359498755 2314 8 -20.39765677240783 2314 9 -21.638488726214067 2314 10-11 -22.037634913127647 2314 12-13 -21.961465542650043 2314 14-15 -21.89263013505891 2314 16-17 -20.710993235636693 2314 18-19 -19.66084477496078 2314 20-21 -19.329603990554865 2314 22-23 -18.95866669481214 2314 24-25 -18.808201165764526 2314 26-27 -18.534117772476463 2314 28-29 -18.341428496743163 2314 30-31 -18.333626703939434 2314 32-33 -18.226228951493685 2314 34-35 -17.900822649572117 2314 36-37 -17.476541599205866 2314 38-39 -17.35961009335998 2314 40-41 -17.235596802672735 2314 42-43 -17.10499299876922 2314 44-45 -16.74262274118306 2314 46-47 -16.4230147484099 2314 48-49 -16.180929026017107 2314 50-51 -14.012712401701503 2314 52-53 -15.855033889661382 2314 54-55 -16.60997408702837 2314 56-57 -16.573821848418422 2314 58-59 -16.30994320773572 2314 60-61 -16.027366554458307 2314 62-63 -15.59956579122598 2314 64-65 -15.252255900019335 2314 66-67 -14.900298978948069 2314 68-69 -14.480105447327201 2314 70-71 -14.119885983613283 2314 72-73 -13.739856848738688 2314 74-75 -13.27513542660043 2314 76-77 -12.862156953363469 2314 78-79 -12.471245733888205 2314 80-81 -12.045294763673057 2314 82-83 -11.587871733748552 2314 84-85 -11.191283315381995 2314 86-87 -10.704629398545995 2314 88-89 -10.295242826192885 2314 90-91 -9.810696755697535 2314 92-93 -9.255638218561067 2314 94-95 -8.699858182871333 2314 96-97 -8.06796920401622 2314 98-99 -7.413695092828828 2314 100-101 -6.505523239205319 2315 1 -18.619115622219052 2315 2 -17.34402829524186 2315 3 -17.983354835719357 2315 4 -19.458266209812987 2315 5 -18.785983707441698 2315 6 -19.757424669181592 2315 7 -19.943457359498755 2315 8 -20.39765677240783 2315 9 -21.638488726214067 2315 10-11 -22.037634913127647 2315 12-13 -21.961465542650043 2315 14-15 -21.89263013505891 2315 16-17 -20.710993235636693 2315 18-19 -19.66084477496078 2315 20-21 -19.329603990554865 2315 22-23 -18.95866669481214 2315 24-25 -18.808201165764526 2315 26-27 -18.534117772476463 2315 28-29 -18.341428496743163 2315 30-31 -18.333626703939434 2315 32-33 -18.226228951493685 2315 34-35 -17.900822649572117 2315 36-37 -17.476541599205866 2315 38-39 -17.35961009335998 2315 40-41 -17.235596802672735 2315 42-43 -17.10499299876922 2315 44-45 -16.74262274118306 2315 46-47 -16.4230147484099 2315 48-49 -16.180929026017107 2315 50-51 -14.012712401701503 2315 52-53 -15.855033889661382 2315 54-55 -16.60997408702837 2315 56-57 -16.573821848418422 2315 58-59 -16.30994320773572 2315 60-61 -16.027366554458307 2315 62-63 -15.59956579122598 2315 64-65 -15.252255900019335 2315 66-67 -14.900298978948069 2315 68-69 -14.480105447327201 2315 70-71 -14.119885983613283 2315 72-73 -13.739856848738688 2315 74-75 -13.27513542660043 2315 76-77 -12.862156953363469 2315 78-79 -12.471245733888205 2315 80-81 -12.045294763673057 2315 82-83 -11.587871733748552 2315 84-85 -11.191283315381995 2315 86-87 -10.704629398545995 2315 88-89 -10.295242826192885 2315 90-91 -9.810696755697535 2315 92-93 -9.255638218561067 2315 94-95 -8.699858182871333 2315 96-97 -8.06796920401622 2315 98-99 -7.413695092828828 2315 100-101 -6.505523239205319 2316 1 -18.619115622219052 2316 2 -17.34402829524186 2316 3 -17.983354835719357 2316 4 -19.458266209812987 2316 5 -18.785983707441698 2316 6 -19.757424669181592 2316 7 -19.943457359498755 2316 8 -20.39765677240783 2316 9 -21.638488726214067 2316 10-11 -22.037634913127647 2316 12-13 -21.961465542650043 2316 14-15 -21.89263013505891 2316 16-17 -20.710993235636693 2316 18-19 -19.66084477496078 2316 20-21 -19.329603990554865 2316 22-23 -18.95866669481214 2316 24-25 -18.808201165764526 2316 26-27 -18.534117772476463 2316 28-29 -18.341428496743163 2316 30-31 -18.333626703939434 2316 32-33 -18.226228951493685 2316 34-35 -17.900822649572117 2316 36-37 -17.476541599205866 2316 38-39 -17.35961009335998 2316 40-41 -17.235596802672735 2316 42-43 -17.10499299876922 2316 44-45 -16.74262274118306 2316 46-47 -16.4230147484099 2316 48-49 -16.180929026017107 2316 50-51 -14.012712401701503 2316 52-53 -15.855033889661382 2316 54-55 -16.60997408702837 2316 56-57 -16.573821848418422 2316 58-59 -16.30994320773572 2316 60-61 -16.027366554458307 2316 62-63 -15.59956579122598 2316 64-65 -15.252255900019335 2316 66-67 -14.900298978948069 2316 68-69 -14.480105447327201 2316 70-71 -14.119885983613283 2316 72-73 -13.739856848738688 2316 74-75 -13.27513542660043 2316 76-77 -12.862156953363469 2316 78-79 -12.471245733888205 2316 80-81 -12.045294763673057 2316 82-83 -11.587871733748552 2316 84-85 -11.191283315381995 2316 86-87 -10.704629398545995 2316 88-89 -10.295242826192885 2316 90-91 -9.810696755697535 2316 92-93 -9.255638218561067 2316 94-95 -8.699858182871333 2316 96-97 -8.06796920401622 2316 98-99 -7.413695092828828 2316 100-101 -6.505523239205319 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 672319.0 3 27585.0 4 100026.0 5 53213.0 6 31615.0 7 31415.0 8 30082.0 9 29623.0 10 31430.0 11 33325.0 12 34747.0 13 35300.0 14 35910.0 15 37112.0 16 38492.0 17 39156.0 18 38920.0 19 40364.0 20 42235.0 21 44502.0 22 47071.0 23 50642.0 24 53747.0 25 57735.0 26 61775.0 27 65653.0 28 70549.0 29 76548.0 30 82246.0 31 88908.0 32 95977.0 33 102337.0 34 106416.0 35 101179.0 36 76974.0 37 34133.0 38 4421.0 39 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.06491601167564 21.541274917654395 14.408766692960038 22.98504237770993 2 14.17213588048243 21.298568922937626 42.880913460365484 21.648381736214464 3 18.156109753282923 27.013907339899095 30.8315840529435 23.99839885387448 4 11.61133272675551 17.83707956665154 41.39589626216322 29.155691444429728 5 11.949649447029035 38.63507893051944 36.81002193477062 12.605249687680908 6 28.184930542218805 40.11164458101274 18.86690992894181 12.836514947826643 7 24.850015676220664 33.56412634478497 24.319851446983325 17.266006532011044 8 23.181810986341546 38.25834927131408 21.474612199786726 17.085227542557647 9 24.64741603884204 17.34642117101721 22.400222581483675 35.605940208657074 10-11 22.991653773512503 28.09346011689894 30.20322214567819 18.711663963910368 12-13 23.539452364233007 25.51464990443985 30.36774180353016 20.57815592779698 14-15 20.90543731101784 27.36613368033864 27.82612912696902 23.9022998816745 16-17 20.365316605232266 30.4496051001767 28.52437811689234 20.66070017769869 18-19 20.181891041432635 29.371198833290634 30.44609553089875 20.000814594377985 20-21 20.939374111106908 28.747438095186595 30.447893965531225 19.865293828175275 22-23 20.515015436052693 28.510952566971337 30.21078687439492 20.76324512258105 24-25 20.392016582378876 28.728460384914968 30.48724303726847 20.392279995437686 26-27 20.440376133899886 28.920721869232413 30.19949328554982 20.439408711317878 28-29 20.360913575377445 28.960788029012296 30.360746008360305 20.317552387249954 30-31 20.72711992906663 28.775931311196363 30.262903545266962 20.234045214470047 32-33 20.490315890826945 28.9919795035815 30.049764945261693 20.467939660329865 34-35 20.59522018689917 28.966499838389343 30.11540932219067 20.322870652520823 36-37 20.6390948497912 28.723707407369186 30.15286131089826 20.484336431941355 38-39 20.51865903630359 29.044699319913942 30.185543546761945 20.251098097020524 40-41 20.725914795670665 28.972078966388903 30.112077729086213 20.189928508854223 42-43 20.667720409074544 28.923040237406205 29.889897824068278 20.51934152945097 44-45 20.69319528791425 29.194307853872797 29.68919784639832 20.42329901181464 46-47 20.736343148807915 29.049987856108377 29.509894758230725 20.703774236852983 48-49 20.61707655781033 29.095352576067533 29.654466992190414 20.63310387393172 50-51 20.88092583670706 29.30719979569873 29.20738295865582 20.604491408938394 52-53 20.423944662406456 29.460842546622462 29.3106806036515 20.804532187319577 54-55 20.491554882458885 29.37684733432982 29.41864383956238 20.71295394364892 56-57 20.535447068630887 29.484243963279372 29.405865178728547 20.57444378936119 58-59 20.454902870893026 29.405443342244915 29.554308986039203 20.58534480082286 60-61 20.48412844405793 29.51993265408831 29.397264717558848 20.59867418429491 62-63 20.420284387219862 29.49026575025457 29.327517220581168 20.7619326419444 64-65 20.22266569938026 29.393159832524095 29.468306101579927 20.915868366515713 66-67 20.33102883156751 29.4174497661231 29.198314002882437 21.053207399426952 68-69 20.392043104897777 29.415048238525078 29.253495759342098 20.93941289723505 70-71 20.401447250014545 29.101236945723173 29.42784607062709 21.069469733635195 72-73 20.39740035458697 29.228612706906244 29.28928395081255 21.08470298769424 74-75 20.261963765826447 29.48872718050195 29.07400415487228 21.175304898799318 76-77 20.300848127051736 29.398822169975773 29.214769336911033 21.085560366061454 78-79 20.236109568951463 29.515469771254732 29.021301078453504 21.227119581340304 80-81 20.338195775555885 29.444708786389857 28.891084034728376 21.326011403325886 82-83 20.451949160200332 29.2997989340042 29.081855044784916 21.166396861010547 84-85 20.578407493359965 29.29389308406559 28.891515364884572 21.236184057689865 86-87 20.264900306428203 29.328844816718224 29.093827793064857 21.31242708378871 88-89 20.435456219106285 29.299174033502222 28.992369972915462 21.272999774476027 90-91 20.468793178704544 29.25994080581044 29.017041520886412 21.254224494598603 92-93 20.45184214443818 29.26409398511837 28.793607098668268 21.49045677177518 94-95 20.31391039682289 29.21936468884242 28.911912500201968 21.55481241413272 96-97 20.283054664697197 29.16779568640316 28.906108934485292 21.64304071441435 98-99 20.215234185214396 29.12338706039269 28.657470687728566 22.00390806666435 100-101 20.39972755164162 29.2492842202974 28.642423584865856 21.708564643195118 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 155552.0 1 141675.5 2 94600.0 3 48010.0 4 29841.5 5 23780.0 6 22349.0 7 22643.0 8 23080.5 9 22263.5 10 19794.0 11 16405.5 12 12737.0 13 9388.5 14 7091.0 15 5905.0 16 5670.5 17 6283.5 18 7456.0 19 8819.0 20 10183.0 21 11629.5 22 13393.0 23 15252.5 24 17158.0 25 19213.5 26 22068.5 27 25950.0 28 30481.5 29 35998.5 30 42065.0 31 48455.5 32 55734.5 33 63432.0 34 70549.0 35 76401.0 36 82104.0 37 86626.0 38 87594.5 39 86666.0 40 84846.5 41 82606.0 42 80272.0 43 78187.5 44 76587.5 45 74484.5 46 71572.5 47 68260.0 48 64701.5 49 60535.5 50 55828.5 51 50579.0 52 44960.0 53 39497.5 54 34371.0 55 29144.0 56 23741.0 57 18952.5 58 15169.5 59 12070.5 60 9326.0 61 6889.0 62 4924.0 63 3386.5 64 2277.0 65 1497.0 66 935.0 67 586.0 68 365.0 69 210.5 70 133.0 71 88.0 72 57.0 73 43.0 74 27.0 75 15.5 76 16.5 77 10.5 78 8.5 79 7.5 80 4.0 81 2.0 82 1.5 83 2.5 84 2.0 85 0.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.1446106859909 2 29.16198744252428 3 22.953610773558324 4 29.253664389401578 5 32.39568338692357 6 25.005991455263032 7 23.92745269438816 8 19.935607218628203 9 11.791683245586679 10-11 15.425500862615932 12-13 15.498012834004427 14-15 19.61635098866693 16-17 22.948157012998387 18-19 26.920088581361657 20-21 28.97782700671504 22-23 28.572270776907395 24-25 27.09783508749829 26-27 28.539836264281554 28-29 25.509216087211005 30-31 27.38093134867658 32-33 26.366051799971423 34-35 24.427316734441803 36-37 27.421988179934154 38-39 27.53899438800357 40-41 24.571054050607827 42-43 25.42746728895867 44-45 24.13171907141661 46-47 27.417398571575614 48-49 22.83812163268692 50-51 28.413093941409713 52-53 22.00325458922429 54-55 23.13410336335718 56-57 25.248242122388532 58-59 24.536123098289057 60-61 24.105679285081123 62-63 23.90554163523713 64-65 22.441571789156082 66-67 22.94487323463307 68-69 23.936516690811718 70-71 22.43014577671536 72-73 22.295472456972902 74-75 19.588275643812594 76-77 21.841715737709453 78-79 20.962700118446463 80-81 21.986278799312366 82-83 22.85830438743515 84-85 19.81762163806387 86-87 21.36988863574564 88-89 20.2143827187995 90-91 21.336263513420093 92-93 21.310876642081215 94-95 20.37008374210933 96-97 19.278601901595724 98-99 18.696585792262418 100-101 20.352531850729804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2603708.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.26196683304637 #Duplication Level Percentage of deduplicated Percentage of total 1 90.20566586618392 71.49878496038332 2 6.769837707619586 10.731813036929005 3 1.5588005181575275 3.7066078490861227 4 0.5746629361090414 1.8219565832822353 5 0.264989582133388 1.050179773507971 6 0.1495246287064284 0.7110969696751501 7 0.09449492584916482 0.5242897574983375 8 0.06630603244953935 0.4204437235877027 9 0.05079201147408759 0.3623287255958557 >10 0.24854124359891386 3.4429261877186508 >50 0.01171180676234908 0.6159692541878443 >100 0.004430219574721628 0.5581641482844981 >500 9.70085525389736E-5 0.050747985247506074 >1k 9.7008552538715E-5 0.10238021569225235 >5k 0.0 0.0 >10k+ 4.85042762693575E-5 4.402310829323482 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 114508 4.397881790123931 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.840676450661902E-5 0.0 0.0 0.0 0.0 7 3.840676450661902E-5 0.0 0.0 0.0 0.0 8 3.840676450661902E-5 0.0 0.0 0.0 0.0 9 3.840676450661902E-5 0.0 0.0 3.840676450661902E-5 0.0 10-11 3.840676450661902E-5 0.0 0.0 1.3442367577316656E-4 0.0 12-13 3.840676450661902E-5 0.0 0.0 1.5362705802647608E-4 0.0 14-15 3.840676450661902E-5 0.0 0.0 1.5362705802647608E-4 0.0 16-17 9.601691126654756E-5 0.0 0.0 1.5362705802647608E-4 0.0 18-19 1.1522029351985706E-4 0.0 0.0 1.920338225330951E-4 0.0 20-21 1.1522029351985706E-4 0.0 0.0 2.4964396929302363E-4 0.0 22-23 1.1522029351985706E-4 0.0 0.0 3.2645749830626165E-4 0.0 24-25 1.1522029351985706E-4 0.0 0.0 3.6486426281288073E-4 0.0 26-27 1.1522029351985706E-4 0.0 0.0 4.608811740794283E-4 0.0 28-29 1.1522029351985706E-4 0.0 0.0 6.337116143592139E-4 0.0 30-31 1.1522029351985706E-4 0.0 0.0 0.0014402536689982132 0.0 32-33 1.1522029351985706E-4 0.0 0.0 0.003629439245875498 0.0 34-35 1.1522029351985706E-4 0.0 0.0 0.006836404082178185 0.0 36-37 1.1522029351985706E-4 0.0 0.0 0.011483622587479089 0.0 38-39 1.1522029351985706E-4 0.0 0.0 0.02072044945132096 0.0 40-41 1.1522029351985706E-4 0.0 0.0 0.030610191311775357 0.0 42-43 1.1522029351985706E-4 0.0 0.0 0.03980861141111061 0.0 44-45 1.5362705802647608E-4 0.0 0.0 0.04985198032959149 0.0 46-47 1.5362705802647608E-4 0.0 0.0 0.06548353348378544 0.0 48-49 1.5362705802647608E-4 0.0 0.0 0.08128791707825916 0.0 50-51 1.5362705802647608E-4 1.920338225330951E-5 0.0 0.10022245198002233 0.0 52-53 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.12121174878288964 0.0 54-55 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.144006163517568 0.0 56-57 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.17144779675754732 0.0 58-59 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.20382469923662716 0.0 60-61 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.2378338892072383 0.0 62-63 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.27405146813698 0.0 64-65 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.31140204661966703 0.0 66-67 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.3543216059558138 0.0 68-69 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.4047688911352578 0.0 70-71 1.5362705802647608E-4 3.840676450661902E-5 0.0 0.4585575648267778 0.0 72-73 1.920338225330951E-4 3.840676450661902E-5 0.0 0.5139401192453225 0.0 74-75 1.920338225330951E-4 3.840676450661902E-5 0.0 0.5713774355649712 0.0 76-77 1.920338225330951E-4 3.840676450661902E-5 0.0 0.6346909868541326 0.0 78-79 1.920338225330951E-4 3.840676450661902E-5 0.0 0.7083935679423345 0.0 80-81 1.920338225330951E-4 3.840676450661902E-5 0.0 0.7899695357543933 0.0 82-83 1.920338225330951E-4 3.840676450661902E-5 0.0 0.8766728066280858 3.840676450661902E-5 84-85 2.112372047864046E-4 3.840676450661902E-5 0.0 0.9624351117713661 3.840676450661902E-5 86-87 2.3044058703971412E-4 5.761014675992853E-5 0.0 1.0551682446725976 3.840676450661902E-5 88-89 2.3044058703971412E-4 1.1522029351985706E-4 0.0 1.157253424731191 3.840676450661902E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1885 0.0 50.81062 1 GTATCAA 3335 0.0 42.541042 1 TCAACGC 3705 0.0 36.559357 4 ATCAACG 3695 0.0 36.524128 3 CAACGCA 3800 0.0 36.034603 5 TATCAAC 3950 0.0 35.005802 2 AACGCAG 4180 0.0 32.363583 6 ACGCAGA 4740 0.0 27.822647 7 CGCAGAG 4845 0.0 26.65376 8 GTGTAGC 875 0.0 25.755432 1 ACATGGG 3670 0.0 23.051687 3 GTACATG 3835 0.0 22.837835 1 GCAGAGT 5380 0.0 22.363445 9 TACATGG 3845 0.0 21.976624 2 GAGTACT 3685 0.0 20.59085 12-13 GTATACG 230 1.0004242E-6 20.041899 1 CAGAGTA 5365 0.0 19.767706 10-11 GTGGTAT 1140 0.0 18.86986 1 AGAGTAC 4930 0.0 18.491882 10-11 TGGTATC 1055 0.0 18.446096 2 >>END_MODULE