FastQCFastQC Report
Sat 18 Jun 2016
SRR1931042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603708
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47030.18062701347462928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.1616924785728661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.055.3548661
GTATCAA57300.038.3999631
ATCAACG64600.032.8056223
TCAACGC65900.032.2305764
CAACGCA66150.031.9901755
AACGCAG70350.030.3489276
TATCAAC71800.029.8496882
CGCAGAG79700.026.4313648
ACGCAGA80300.026.1156987
ACATGGG58100.022.8178143
TACATGG63550.022.508092
GTACATG63700.022.3813481
GCAGAGT94500.021.688959
GGACCGT1856.622804E-620.5169686
GAGTACT68100.019.50731712-13
GACCGTT2201.3609751E-619.4095177
CAGAGTA93400.019.35373110-11
GTGGTAT17600.019.1712361
AGAGTAC88600.018.98318710-11
TAGACCG2302.0818698E-618.566345
Produced by FastQC (version 0.11.5)