##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931042_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2603708 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34127137144411 31.0 31.0 34.0 30.0 34.0 2 31.499723471295553 31.0 31.0 34.0 30.0 34.0 3 31.57247817343573 31.0 31.0 34.0 30.0 34.0 4 35.338686596192815 37.0 35.0 37.0 33.0 37.0 5 35.218165401035755 37.0 35.0 37.0 33.0 37.0 6 35.24277376725808 37.0 35.0 37.0 32.0 37.0 7 35.12798516577128 37.0 35.0 37.0 32.0 37.0 8 35.13428694769152 37.0 35.0 37.0 32.0 37.0 9 36.641323066949134 39.0 35.0 39.0 32.0 39.0 10-11 36.58710615783337 38.5 35.0 39.0 32.0 39.0 12-13 36.47304690080455 38.0 35.0 39.0 32.0 39.0 14-15 37.545609185054545 39.0 36.0 41.0 32.0 41.0 16-17 37.50065310703043 39.0 36.0 41.0 32.0 41.0 18-19 37.52766208806825 39.0 36.0 41.0 32.0 41.0 20-21 37.50917460790534 39.0 36.0 41.0 32.0 41.0 22-23 37.4111839730108 39.0 36.0 41.0 32.0 41.0 24-25 37.29180000215078 39.0 36.0 41.0 31.5 41.0 26-27 37.048403661240044 39.0 36.0 40.0 31.0 41.0 28-29 36.87139552515106 39.0 36.0 40.0 30.5 41.0 30-31 36.658442114092665 38.5 35.5 40.0 30.0 41.0 32-33 36.505541135949194 38.0 35.0 40.0 30.0 41.0 34-35 36.59432259685033 38.5 35.0 40.0 30.0 41.0 36-37 36.67043885105396 39.0 35.0 40.0 30.0 41.0 38-39 36.66474255177616 39.0 35.0 40.0 30.0 41.0 40-41 36.56672138350383 39.0 35.0 40.0 30.0 41.0 42-43 36.44918285767836 39.0 35.0 40.0 30.0 41.0 44-45 36.305280008357315 39.0 35.0 40.0 30.0 41.0 46-47 36.10564049424897 38.5 35.0 40.0 29.5 41.0 48-49 35.92111999502248 38.5 35.0 40.0 29.0 41.0 50-51 35.68875369281041 38.0 35.0 40.0 28.0 41.0 52-53 35.49510409769452 38.0 34.0 40.0 27.0 41.0 54-55 35.36041387897568 38.0 34.0 40.0 27.0 41.0 56-57 35.21299527443169 38.0 34.0 40.0 27.0 41.0 58-59 35.03095354778647 38.0 34.0 40.0 26.5 41.0 60-61 34.74486463151782 38.0 33.5 40.0 26.0 41.0 62-63 34.513730034243466 37.5 33.0 40.0 26.0 41.0 64-65 34.22519614334634 37.0 33.0 40.0 26.0 41.0 66-67 33.92579755487174 37.0 32.5 39.5 25.0 41.0 68-69 33.59219121345404 36.0 32.0 39.0 24.5 40.5 70-71 33.23839328373228 36.0 32.0 39.0 23.5 40.0 72-73 32.826513764216266 35.5 31.0 38.5 22.5 40.0 74-75 32.30848217234805 35.0 31.0 38.0 22.0 40.0 76-77 30.899994354205617 34.0 29.5 36.0 20.5 39.0 78-79 31.643334429206348 34.5 30.5 37.0 22.0 39.0 80-81 31.602569105291376 34.5 31.0 36.0 22.0 39.0 82-83 31.30869129718079 34.0 31.0 36.0 21.5 37.5 84-85 30.945518660310604 34.0 30.5 35.5 20.5 37.0 86-87 30.578302943340802 34.0 30.0 35.0 20.0 37.0 88-89 30.214689588847904 34.0 30.0 35.0 18.0 36.0 90-91 29.864051959743563 34.0 30.0 35.0 17.5 36.0 92-93 29.540932969442043 34.0 29.0 35.0 9.5 36.0 94-95 29.144582072951344 34.0 29.0 35.0 4.5 35.0 96-97 28.712151669849305 34.0 29.0 35.0 2.0 35.0 98-99 28.21288427888227 34.0 29.0 35.0 2.0 35.0 100-101 27.06877172862702 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.393114407652444 1101 2 0.3737202047032646 1101 3 0.3298843267663436 1101 4 0.13059729467451575 1101 5 0.17621282029787722 1101 6 0.24731913849880272 1101 7 0.43085430614851106 1101 8 0.32742505978121983 1101 9 0.4815388220909824 1101 10-11 0.4629977105123473 1101 12-13 0.4737726929261754 1101 14-15 0.5600290634121947 1101 16-17 0.630102048782156 1101 18-19 0.5761159840049785 1101 20-21 0.5296503862830875 1101 22-23 0.5206654751887214 1101 24-25 0.5590718934471397 1101 26-27 0.5185672202262097 1101 28-29 0.5682444302916849 1101 30-31 0.46898644298791226 1101 32-33 0.34681687326472144 1101 34-35 0.5471595972767815 1101 36-37 0.5580280626600995 1101 38-39 0.5300478524167858 1101 40-41 0.5415871612582919 1101 42-43 0.5356535613321256 1101 44-45 0.5461661092060908 1101 46-47 0.5597298970129145 1101 48-49 0.5025751221073946 1101 50-51 0.5398880713848726 1101 52-53 0.41221249154775563 1101 54-55 0.4475100502715321 1101 56-57 0.5813557405751624 1101 58-59 0.39609297492498285 1101 60-61 0.3930104069104985 1101 62-63 0.18567145828659193 1101 64-65 0.29701058053869644 1101 66-67 0.3558144395912919 1101 68-69 0.29099697824415216 1101 70-71 0.2439644941574457 1101 72-73 0.3183823425558714 1101 74-75 0.2893804765549248 1101 76-77 0.20796389037210972 1101 78-79 0.2840344941583872 1101 80-81 0.3357935080370815 1101 82-83 0.4067048824490449 1101 84-85 0.33340513014450934 1101 86-87 0.4240610226777548 1101 88-89 0.3512035006857097 1101 90-91 0.32960244860477417 1101 92-93 0.16706396619889574 1101 94-95 0.48606660166035454 1101 96-97 0.6012202917678842 1101 98-99 0.5908004317166089 1101 100-101 0.8214697258415136 1102 1 0.30292085197277174 1102 2 0.310167428476948 1102 3 0.3456028299180218 1102 4 0.14686573488125987 1102 5 0.22292228508860745 1102 6 0.3102086657025396 1102 7 0.33687657402798266 1102 8 0.3152364044978313 1102 9 0.46344963310545495 1102 10-11 0.39866878886549983 1102 12-13 0.4097440895501947 1102 14-15 0.5211123590672244 1102 16-17 0.48132052644556467 1102 18-19 0.47347653548240487 1102 20-21 0.40588698277944246 1102 22-23 0.4362782574435684 1102 24-25 0.550615745455616 1102 26-27 0.5883178009154122 1102 28-29 0.5559819421492733 1102 30-31 0.44739414110931364 1102 32-33 0.4666830654063574 1102 34-35 0.49703872194321974 1102 36-37 0.5628309386231081 1102 38-39 0.5884713920499394 1102 40-41 0.5522963888973322 1102 42-43 0.6413474461428947 1102 44-45 0.6237597342581722 1102 46-47 0.5585413680626701 1102 48-49 0.5946444202713508 1102 50-51 0.5037540208338882 1102 52-53 0.4734375241284141 1102 54-55 0.6883508971025663 1102 56-57 0.5743288748340589 1102 58-59 0.6686409482753319 1102 60-61 0.5421018056179108 1102 62-63 0.36459590330729696 1102 64-65 0.39573564082398605 1102 66-67 0.36826282585156633 1102 68-69 0.4677280471112226 1102 70-71 0.6122391933959008 1102 72-73 0.5903518430744938 1102 74-75 0.46774057810904424 1102 76-77 0.4502336038839907 1102 78-79 0.6007748274562594 1102 80-81 0.4963821927982046 1102 82-83 0.4470976898818684 1102 84-85 0.5343676446617387 1102 86-87 0.4645247539826656 1102 88-89 0.49826761254152174 1102 90-91 0.5888918135701573 1102 92-93 0.6644289781229737 1102 94-95 0.7449949751986615 1102 96-97 0.7953026365997502 1102 98-99 0.823882733329242 1102 100-101 0.9128317246092088 1103 1 0.29620175705003504 1103 2 0.3650915725275041 1103 3 0.36625923817669204 1103 4 0.21379183755190923 1103 5 0.241720439008013 1103 6 0.31017349710971587 1103 7 0.385434391194579 1103 8 0.4377058889803678 1103 9 0.41043676681103847 1103 10-11 0.3962985609730154 1103 12-13 0.41664565536416376 1103 14-15 0.5315563490252373 1103 16-17 0.5682908333321208 1103 18-19 0.5426047331190773 1103 20-21 0.5373953366728799 1103 22-23 0.43511444754874873 1103 24-25 0.6413716808319734 1103 26-27 0.673893673096515 1103 28-29 0.570206691099628 1103 30-31 0.6089115847313593 1103 32-33 0.6246273799975341 1103 34-35 0.6436691649592845 1103 36-37 0.7380355927805837 1103 38-39 0.4952808432034672 1103 40-41 0.5496510343979111 1103 42-43 0.5881717222109373 1103 44-45 0.6841201311763214 1103 46-47 0.6431813498832142 1103 48-49 0.5915257776708174 1103 50-51 0.6503150735110239 1103 52-53 0.5523347452685243 1103 54-55 0.63170565622476 1103 56-57 0.6086900779245639 1103 58-59 0.6381664157470937 1103 60-61 0.6128677775696048 1103 62-63 0.48987725204987953 1103 64-65 0.5112801588519531 1103 66-67 0.46751048849278476 1103 68-69 0.3923236082937507 1103 70-71 0.5123534913225782 1103 72-73 0.575163880472239 1103 74-75 0.5372166742870235 1103 76-77 0.538403295049644 1103 78-79 0.5553735267593787 1103 80-81 0.5213113145572841 1103 82-83 0.4417642375163773 1103 84-85 0.3454178870544986 1103 86-87 0.4282933933368618 1103 88-89 0.4975691101825177 1103 90-91 0.4370971332256133 1103 92-93 0.29590432764468133 1103 94-95 0.4616424344030925 1103 96-97 0.5092908091314534 1103 98-99 0.5505320050688454 1103 100-101 0.8735250057848134 1104 1 0.34184113273459005 1104 2 0.36169483047909523 1104 3 0.3788072913519578 1104 4 0.1994066228964968 1104 5 0.27033932632197377 1104 6 0.27680070111289723 1104 7 0.23293763948184676 1104 8 0.4210153299528159 1104 9 0.533165784486016 1104 10-11 0.2995655728708755 1104 12-13 0.47431463341540336 1104 14-15 0.5191154087937235 1104 16-17 0.5010802798883915 1104 18-19 0.5141122874003727 1104 20-21 0.48881089194208727 1104 22-23 0.4702364865067281 1104 24-25 0.4636276163387052 1104 26-27 0.5640559935011282 1104 28-29 0.57862116450125 1104 30-31 0.47813097566407237 1104 32-33 0.5457774884527495 1104 34-35 0.5977197926035487 1104 36-37 0.5395906767528373 1104 38-39 0.5795184621283624 1104 40-41 0.4376372707873202 1104 42-43 0.5369485138695538 1104 44-45 0.4869919124189366 1104 46-47 0.3732795501709987 1104 48-49 0.412527431344337 1104 50-51 0.34248482202379904 1104 52-53 0.3162833203173818 1104 54-55 0.4921887651309689 1104 56-57 0.5569341907394616 1104 58-59 0.5271284805839827 1104 60-61 0.39974187825040275 1104 62-63 0.26046495043701157 1104 64-65 0.4177149890819578 1104 66-67 0.252440717429927 1104 68-69 0.230579170650131 1104 70-71 0.432343008213671 1104 72-73 0.4262342045492957 1104 74-75 0.4040265608922695 1104 76-77 0.39816218354479815 1104 78-79 0.5373717066446879 1104 80-81 0.3954240757625307 1104 82-83 0.4402253278670827 1104 84-85 0.45444611225476805 1104 86-87 0.37415686057479647 1104 88-89 0.38903094683393746 1104 90-91 0.3858045296562551 1104 92-93 0.37853484973303253 1104 94-95 0.4804646483927364 1104 96-97 0.40209424211472466 1104 98-99 0.686005114082441 1104 100-101 0.9255736415041618 1105 1 0.25661243282285895 1105 2 0.2988796851844988 1105 3 0.2992095067096976 1105 4 0.07398578392988497 1105 5 0.17051082468061196 1105 6 0.34074027090765924 1105 7 0.29786593642230486 1105 8 0.35468425832954864 1105 9 0.3950672167624063 1105 10-11 0.503519746535666 1105 12-13 0.44342931185335743 1105 14-15 0.4887776667399564 1105 16-17 0.5366388267350857 1105 18-19 0.5381488296218038 1105 20-21 0.42151858697946665 1105 22-23 0.46995977676598955 1105 24-25 0.5493204956922071 1105 26-27 0.5924601138712404 1105 28-29 0.6067088108188301 1105 30-31 0.2922820968470603 1105 32-33 0.4720652156637968 1105 34-35 0.6434131863996342 1105 36-37 0.6145250787618863 1105 38-39 0.5795550111740368 1105 40-41 0.5026013293613332 1105 42-43 0.45248511291588756 1105 44-45 0.5692194566721582 1105 46-47 0.312877823008769 1105 48-49 0.4296743800305265 1105 50-51 0.4026303777779532 1105 52-53 0.25748286130861686 1105 54-55 0.4265242378630205 1105 56-57 0.546938190793 1105 58-59 0.6156203475354758 1105 60-61 0.5686906620792698 1105 62-63 0.32990452823712957 1105 64-65 0.2346985628778313 1105 66-67 0.4061911350864307 1105 68-69 0.3292656622540875 1105 70-71 0.2277850206920462 1105 72-73 0.5035580742388888 1105 74-75 0.5046562543606115 1105 76-77 0.42984895940452716 1105 78-79 0.4942941615121974 1105 80-81 0.5559906607745582 1105 82-83 0.6028404311138722 1105 84-85 0.56341620608017 1105 86-87 0.4689900197588166 1105 88-89 0.5687932612376194 1105 90-91 0.6545967103389891 1105 92-93 0.6896318617927726 1105 94-95 0.7050937707248224 1105 96-97 0.7144365464342819 1105 98-99 0.6813782804949042 1105 100-101 0.7998709746180701 1106 1 0.18617477863276832 1106 2 0.24908966816863654 1106 3 0.19839000415214514 1106 4 0.08614953962554495 1106 5 0.14200873778075618 1106 6 0.11489736507417092 1106 7 0.20140720118188682 1106 8 0.19891515686018124 1106 9 0.26019607700993674 1106 10-11 0.240086912644621 1106 12-13 0.22231244546149753 1106 14-15 0.39166532067145 1106 16-17 0.351045805824711 1106 18-19 0.3601274796277565 1106 20-21 0.19106122748963372 1106 22-23 0.2437845702741015 1106 24-25 0.3339036846406884 1106 26-27 0.31754713268178136 1106 28-29 0.2759210901686089 1106 30-31 0.3528440857893358 1106 32-33 0.39325306768268575 1106 34-35 0.2840455403295721 1106 36-37 0.25339080860791796 1106 38-39 0.26407316228937816 1106 40-41 0.07583729127476602 1106 42-43 0.10509671163116963 1106 44-45 0.1326045789122574 1106 46-47 0.2081252770943891 1106 48-49 0.16120415261791976 1106 50-51 -0.011883403001881732 1106 52-53 0.05045683583136906 1106 54-55 0.13877294357134673 1106 56-57 0.2580830635360698 1106 58-59 0.3128450551884825 1106 60-61 0.24335603402326456 1106 62-63 0.08050357452131607 1106 64-65 0.022251381873530818 1106 66-67 -0.06948432741919675 1106 68-69 0.09377039204322557 1106 70-71 0.14178509309997622 1106 72-73 0.1764493227066879 1106 74-75 0.2921018837665059 1106 76-77 0.27504236614570843 1106 78-79 0.19727206675091224 1106 80-81 0.07390864845486789 1106 82-83 0.07651614187524203 1106 84-85 0.3218699606897104 1106 86-87 0.29275197353153004 1106 88-89 0.16736915250160322 1106 90-91 0.05945748024211639 1106 92-93 0.08367411181734141 1106 94-95 0.03733805437769888 1106 96-97 0.05249066101465516 1106 98-99 0.32840309055339745 1106 100-101 0.4900466414174822 1107 1 0.09557720636707145 1107 2 0.17990053349506852 1107 3 0.1647738489095545 1107 4 0.060685999602064555 1107 5 0.15397595767845473 1107 6 0.08588764689616113 1107 7 0.15839792779541995 1107 8 0.08006168464154939 1107 9 0.18981919375737277 1107 10-11 0.20912722317118693 1107 12-13 0.1912725900704686 1107 14-15 0.2276346041950248 1107 16-17 0.2515920977631225 1107 18-19 0.2117649331444511 1107 20-21 0.2531145610988048 1107 22-23 0.26561038783716384 1107 24-25 0.12408685083239845 1107 26-27 0.24630626771740083 1107 28-29 0.26522786186124847 1107 30-31 0.34817713638695835 1107 32-33 0.3532657907895995 1107 34-35 0.3646568959610974 1107 36-37 0.34044168551962173 1107 38-39 0.2984622721373711 1107 40-41 0.3401282883836174 1107 42-43 0.27096102952264545 1107 44-45 0.23115331720415355 1107 46-47 0.18581096463351088 1107 48-49 0.2830172987940074 1107 50-51 0.25902865240052364 1107 52-53 0.2661097484401722 1107 54-55 0.44395773894138557 1107 56-57 0.31886486225238286 1107 58-59 0.24124939138882695 1107 60-61 0.2419473119714297 1107 62-63 0.03802818028601962 1107 64-65 0.0044685125543253434 1107 66-67 0.047456411865724135 1107 68-69 0.178654591082271 1107 70-71 0.3559302399587594 1107 72-73 0.32688661485715187 1107 74-75 0.43168716737282864 1107 76-77 0.2968511161263123 1107 78-79 0.3692042981045809 1107 80-81 0.22870813022100123 1107 82-83 0.3354069300355107 1107 84-85 0.17628464605141048 1107 86-87 0.40392891264163566 1107 88-89 0.367139774537403 1107 90-91 0.4214910309716302 1107 92-93 0.2545132420947134 1107 94-95 0.4797163444128181 1107 96-97 0.4897780273832417 1107 98-99 0.5703071086760687 1107 100-101 0.7784834836044077 1108 1 0.10003066666265781 1108 2 0.18239788529651335 1108 3 0.10474560833451108 1108 4 0.04441414745147654 1108 5 0.05491089811848582 1108 6 0.08662615134214491 1108 7 0.06596703107980062 1108 8 0.12409398080246348 1108 9 0.2382613722629756 1108 10-11 0.0896136614034333 1108 12-13 0.08299698248037402 1108 14-15 0.12307356566798688 1108 16-17 0.11961794954445537 1108 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2316 68-69 -0.8156584018462496 2316 70-71 -0.7856946664811986 2316 72-73 -1.5718884615223452 2316 74-75 -2.731105544414362 2316 76-77 -1.7917777918317306 2316 78-79 -1.5318697324444024 2316 80-81 -1.2408055547173653 2316 82-83 -1.1237334723413177 2316 84-85 -1.0896043662150738 2316 86-87 -1.1186467820966826 2316 88-89 -0.9367592587752505 2316 90-91 -1.0196011729332852 2316 92-93 -1.0937381797010595 2316 94-95 -1.4616691257743604 2316 96-97 -1.32280035005504 2316 98-99 -1.4632252326450441 2316 100-101 -1.8506232125132804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 13.0 4 16.0 5 41.0 6 92.0 7 294.0 8 763.0 9 1525.0 10 2610.0 11 4205.0 12 5846.0 13 7693.0 14 9550.0 15 11854.0 16 13916.0 17 16233.0 18 14391.0 19 11653.0 20 11429.0 21 12484.0 22 13796.0 23 16105.0 24 20153.0 25 25877.0 26 33570.0 27 43441.0 28 55920.0 29 70300.0 30 86139.0 31 105766.0 32 127378.0 33 154814.0 34 191085.0 35 238910.0 36 312529.0 37 435538.0 38 453840.0 39 93916.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.17639336404242 22.698000017668026 15.218893420695329 22.90671319759423 2 14.444783299059512 22.400137965894153 41.56583608023224 21.589242654814093 3 17.93408563590128 27.239595475737723 31.559529291752238 23.26678959660876 4 11.994061313590034 18.755915217903162 40.84566648250942 28.40435698599738 5 11.537889853393654 39.20935275589281 37.127958215405435 12.124799175308096 6 28.07915053095656 41.204448904768874 18.33162614543858 12.384774418835987 7 24.62696455476749 33.837908796957564 24.2028384914278 17.33228815684714 8 23.05861766741458 39.054555733679095 21.092289930506215 16.794536668400106 9 24.168301514609166 17.107141046538242 22.220118385010913 36.50443905384167 10-11 22.84455476574178 28.456839246182753 30.06713886503402 18.63146712304145 12-13 23.326540456917595 25.65689009673896 30.427298299194838 20.589271147148605 14-15 20.532525152590075 27.645016261424093 27.84152447202221 23.980934113963624 16-17 19.897891775882705 30.87383454673105 28.70006928580317 20.52820439158308 18-19 19.839340663392363 29.756332123264208 30.657431632118502 19.74689558122493 20-21 20.476988971113506 29.051279943833947 30.739852548749706 19.73187853630284 22-23 20.172758031408772 28.75324130146355 30.635697399027805 20.438303268099872 24-25 20.03662491569663 28.97306977937483 30.78651391946513 20.20379138546341 26-27 20.142139275211832 29.15894591559866 30.57603700352986 20.122877805659645 28-29 20.094469263787282 29.090883602744398 30.70145454321997 20.113192590248357 30-31 20.469032216557952 28.90233632699936 30.59993988982362 20.028691566619074 32-33 20.270735659820456 29.10052046229083 30.426828249364764 20.201915628523953 34-35 20.307728532983838 29.093700232698744 30.555634513164883 20.042936721152536 36-37 20.366668356577286 28.960190314661354 30.566546375756236 20.10659495300512 38-39 20.20534996043133 29.177787853177485 30.59273499669605 20.024127189695133 40-41 20.335158582351095 29.098523887307998 30.602289927690336 19.964027602650575 42-43 20.362411820300185 29.076124689086797 30.321333154337974 20.24013033627504 44-45 20.341633962514173 29.32409711092781 30.17511885362783 20.159150072930178 46-47 20.384889577206845 29.153165340732752 30.00797404058003 20.453971041480376 48-49 20.309332620431363 29.235107272349104 30.098886340341167 20.356673766878366 50-51 20.428398901864902 29.36845687930384 29.80679894850236 20.3963452703289 52-53 20.49953590381866 29.435309913971103 29.656495770072834 20.408658412137402 54-55 20.51296628606209 29.41599806858334 29.701773211811833 20.36926243354273 56-57 20.50569706187933 29.51116462809219 29.60073515431903 20.38240315570945 58-59 20.41395115497758 29.412784922254993 29.84658815467104 20.32667576809639 60-61 20.4138736441215 29.579473860692907 29.74707444026751 20.25957805491808 62-63 20.43217999100692 29.457581599906373 29.72970319747208 20.380535211614628 64-65 20.286929899737967 29.372651973478032 29.75661374439149 20.583804382392508 66-67 20.377652647815545 29.452582512488824 29.6050589845221 20.564705855173536 68-69 20.374611997039974 29.508168949444684 29.696905293781096 20.420313759734242 70-71 20.439875015471003 29.153547994178474 29.77342752535368 20.633149464996844 72-73 20.457345771929912 29.402610381901738 29.633497118531988 20.50654672763636 74-75 20.315388079960396 29.59978017496271 29.49225538447141 20.592576360605488 76-77 20.25361033130914 29.476552956272673 29.63716179965038 20.632674912767808 78-79 20.27474982893671 29.51333909593698 29.539821978949277 20.672089096177036 80-81 20.372035020076186 29.50470799992242 29.43889797102707 20.684359008974322 82-83 20.448087880822275 29.395097299697206 29.60959907946667 20.547215740013858 84-85 20.553130381747877 29.37395437583631 29.401952907161633 20.670962335254185 86-87 20.20384006194243 29.4716803881234 29.58590210576608 20.738577444168087 88-89 20.41815020938428 29.34312271491534 29.516663846967333 20.722063228733052 90-91 20.431080143173578 29.413516836415464 29.566653722816472 20.58874929759448 92-93 20.44086063223303 29.403526132152553 29.489265145208932 20.66634809040549 94-95 20.213969673226714 29.37018942710974 29.481610360879383 20.934230538784163 96-97 20.283849142779893 29.34080140353734 29.53212477440818 20.843224679274588 98-99 20.17593754752837 29.310794451605172 29.508070797493417 21.005197203373037 100-101 20.196850030802224 29.322258870810398 29.418621442957505 21.062269655429873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 110.0 1 83.5 2 70.5 3 97.5 4 93.0 5 101.0 6 175.0 7 371.5 8 655.5 9 889.0 10 908.5 11 874.0 12 973.5 13 1121.0 14 1331.0 15 1574.0 16 1791.5 17 2017.5 18 2360.0 19 2772.5 20 3354.0 21 4085.0 22 5043.5 23 6585.0 24 8622.5 25 11266.0 26 15631.5 27 21413.5 28 28283.0 29 37626.5 30 47654.5 31 57849.0 32 70172.0 33 83242.0 34 95055.0 35 103878.5 36 114244.0 37 122352.0 38 122123.0 39 119746.5 40 116817.5 41 113319.0 42 109684.5 43 107452.5 44 106289.5 45 104615.5 46 101092.5 47 96172.5 48 91801.5 49 86490.5 50 79556.5 51 71127.5 52 62514.0 53 54600.0 54 46866.5 55 38947.0 56 31253.5 57 24342.0 58 19003.0 59 15059.5 60 11045.5 61 7434.5 62 4844.5 63 2960.5 64 1731.5 65 972.5 66 532.0 67 282.5 68 145.0 69 80.5 70 45.0 71 26.0 72 14.5 73 9.5 74 9.0 75 11.5 76 8.0 77 2.5 78 1.5 79 1.5 80 1.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005184913208393568 2 0.006375522908098758 3 0.0021891855768772844 4 0.17206230498965322 5 0.0026500667509567126 6 4.2247440957280926E-4 7 3.840676450661902E-5 8 8.449488191456185E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.264574983062617E-4 24-25 6.145082321059043E-4 26-27 0.0024772363106769267 28-29 1.5362705802647608E-4 30-31 0.005972251880779258 32-33 0.0207972629803342 34-35 0.013135113461263705 36-37 7.681352901323804E-4 38-39 2.4964396929302363E-4 40-41 0.001728304402797856 42-43 0.011080351560159588 44-45 0.007316488638510924 46-47 0.009985758771720945 48-49 0.010158589212000732 50-51 0.18967180651593804 52-53 0.11345358235255258 54-55 0.016802959471645822 56-57 0.006644370259645091 58-59 0.017436671086005037 60-61 0.12213351113104849 62-63 0.23643204230274667 64-65 0.2425003110947925 66-67 0.2529469510405929 68-69 0.2471091228355868 70-71 0.23453090745966906 72-73 0.15996417417006822 74-75 0.2725920110857285 76-77 0.21008500185120604 78-79 0.005357743648673354 80-81 5.7610146759928536E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.601691126654756E-5 90-91 0.005953048498525949 92-93 0.017628704908538132 94-95 0.0026116599864500936 96-97 3.840676450661902E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2603708.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.27842834301397 #Duplication Level Percentage of deduplicated Percentage of total 1 75.69155691756133 45.62568089826383 2 14.956635959900593 18.031250179228277 3 4.459371108362414 8.064116454309916 4 1.873423678980016 4.5170813995801 5 0.9176584017454045 2.7657503106487544 6 0.5363692673240422 1.9398897867472307 7 0.32983005896255885 1.3917146302182672 8 0.2220832998981984 1.0709465823274904 9 0.1453994889624444 0.78880074088802 >10 0.7715626283081926 8.450254487332632 >50 0.06031023376713105 2.5523723168961463 >100 0.034138519404106865 3.7249972379496255 >500 0.0013411220498567987 0.5234244749495451 >1k 3.1931477376511054E-4 0.5537205006601623 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4703 0.18062701347462928 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4210 0.1616924785728661 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.840676450661902E-5 0.0 0.0 0.0 0.0 9 3.840676450661902E-5 0.0 0.0 0.0 0.0 10-11 3.840676450661902E-5 0.0 0.0 3.840676450661902E-5 0.0 12-13 3.840676450661902E-5 0.0 0.0 3.840676450661902E-5 0.0 14-15 3.840676450661902E-5 0.0 0.0 3.840676450661902E-5 0.0 16-17 3.840676450661902E-5 0.0 0.0 3.840676450661902E-5 0.0 18-19 3.840676450661902E-5 0.0 0.0 3.840676450661902E-5 0.0 20-21 3.840676450661902E-5 0.0 0.0 7.681352901323804E-5 0.0 22-23 3.840676450661902E-5 0.0 0.0 1.5362705802647608E-4 0.0 24-25 3.840676450661902E-5 0.0 0.0 2.3044058703971412E-4 0.0 26-27 3.840676450661902E-5 0.0 0.0 3.6486426281288073E-4 0.0 28-29 3.840676450661902E-5 0.0 0.0 6.529149966125234E-4 0.0 30-31 3.840676450661902E-5 0.0 0.0 0.0020163551365974986 0.0 32-33 3.840676450661902E-5 0.0 0.0 0.005933845116272639 0.0 34-35 3.840676450661902E-5 0.0 0.0 0.011675656410012183 0.0 36-37 3.840676450661902E-5 0.0 0.0 0.021123720478640463 0.0 38-39 3.840676450661902E-5 0.0 0.0 0.039347730237031184 0.0 40-41 3.840676450661902E-5 0.0 0.0 0.059242434251459836 0.0 42-43 3.840676450661902E-5 0.0 0.0 0.07963642620447453 0.0 44-45 3.840676450661902E-5 0.0 0.0 0.10354463710984488 0.0 46-47 3.840676450661902E-5 0.0 0.0 0.135691099001885 0.0 48-49 3.840676450661902E-5 0.0 0.0 0.16804879809871154 0.0 50-51 3.840676450661902E-5 0.0 0.0 0.20447761423323968 0.0 52-53 3.840676450661902E-5 0.0 0.0 0.2405223627227016 0.0 54-55 3.840676450661902E-5 0.0 0.0 0.2803885842805722 0.0 56-57 3.840676450661902E-5 0.0 0.0 0.3300485307876305 0.0 58-59 3.840676450661902E-5 0.0 0.0 0.38654488137686716 0.0 60-61 3.840676450661902E-5 0.0 0.0 0.44753482341337814 0.0 62-63 3.840676450661902E-5 0.0 0.0 0.511366866023379 0.0 64-65 3.840676450661902E-5 0.0 0.0 0.5770808400942041 0.0 66-67 3.840676450661902E-5 0.0 0.0 0.6522812849981642 0.0 68-69 3.840676450661902E-5 0.0 0.0 0.7425371815887188 0.0 70-71 5.761014675992853E-5 0.0 0.0 0.8397447025549716 0.0 72-73 1.1522029351985706E-4 0.0 0.0 0.9416762555555385 0.0 74-75 1.1522029351985706E-4 0.0 0.0 1.045816197515236 0.0 76-77 1.3442367577316656E-4 0.0 0.0 1.1634369138167566 0.0 78-79 1.5362705802647608E-4 0.0 0.0 1.3008947239859463 0.0 80-81 1.5362705802647608E-4 0.0 0.0 1.4534847993707434 0.0 82-83 1.5362705802647608E-4 0.0 0.0 1.6150428542678366 0.0 84-85 1.5362705802647608E-4 0.0 0.0 1.7780219594516744 0.0 86-87 1.5362705802647608E-4 0.0 0.0 1.955691652059294 0.0 88-89 1.5362705802647608E-4 0.0 0.0 2.15243030324445 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3125 0.0 55.354866 1 GTATCAA 5730 0.0 38.399963 1 ATCAACG 6460 0.0 32.805622 3 TCAACGC 6590 0.0 32.230576 4 CAACGCA 6615 0.0 31.990175 5 AACGCAG 7035 0.0 30.348927 6 TATCAAC 7180 0.0 29.849688 2 CGCAGAG 7970 0.0 26.431364 8 ACGCAGA 8030 0.0 26.115698 7 ACATGGG 5810 0.0 22.817814 3 TACATGG 6355 0.0 22.50809 2 GTACATG 6370 0.0 22.381348 1 GCAGAGT 9450 0.0 21.68895 9 GGACCGT 185 6.622804E-6 20.516968 6 GAGTACT 6810 0.0 19.507317 12-13 GACCGTT 220 1.3609751E-6 19.409517 7 CAGAGTA 9340 0.0 19.353731 10-11 GTGGTAT 1760 0.0 19.171236 1 AGAGTAC 8860 0.0 18.983187 10-11 TAGACCG 230 2.0818698E-6 18.56634 5 >>END_MODULE