FastQCFastQC Report
Sat 18 Jun 2016
SRR1931041_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931041_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286103
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1473514.48406516776863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.058.7841531
GTATCAA49050.044.2266581
ATCAACG59700.035.3878143
CAACGCA60850.034.0475435
TCAACGC62700.033.772714
TATCAAC61950.033.687212
AACGCAG67850.030.9029856
GTACATG62850.028.2327791
CGCAGAG76150.026.023178
ACGCAGA75600.025.8538687
TACATGG68350.025.1314892
ACATGGG66750.024.2475473
GAGTACA48300.023.1468641
GCAGAGT82150.022.6644829
GAGTACT45450.022.29976812-13
AGTACAT50650.020.6520252
CAGAGTA78600.019.89564710-11
AGAGTAC71000.019.4454910-11
GTACTAG7150.019.3660281
GTGGTAT13550.018.9240251
Produced by FastQC (version 0.11.5)