FastQCFastQC Report
Sat 18 Jun 2016
SRR1931041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286103
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79440.24174531352182207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66480.2023065010439417No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA39730.12090308794337852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.11941196000247101No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA36550.11122597191871346No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC34540.10510930424274588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47850.059.8940121
GTATCAA90050.039.5317571
CAACGCA111600.031.4554125
ATCAACG112550.031.2877563
TATCAAC115500.030.5737192
TCAACGC117400.030.156664
AACGCAG120800.029.3346926
ACGCAGA136200.025.5998927
CGCAGAG135700.025.589348
GTACATG109350.025.2090931
ACATGGG109800.023.3718533
TACATGG117800.022.9566442
GCAGAGT152000.022.1274059
GAGTACT92600.021.31131212-13
GTGGTAT25850.021.1439111
CAGAGTA149400.020.25806410-11
CATGGGT18750.020.022624
AGAGTAC141050.019.9943210-11
GAGTACA85100.019.2680421
GTACTTT103800.019.1032314-15
Produced by FastQC (version 0.11.5)