##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931041_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3286103 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.346291945200743 31.0 31.0 34.0 30.0 34.0 2 31.50819922564813 31.0 31.0 34.0 30.0 34.0 3 31.570174154614143 31.0 31.0 34.0 30.0 34.0 4 35.34718266591157 37.0 35.0 37.0 33.0 37.0 5 35.22109166998113 37.0 35.0 37.0 33.0 37.0 6 35.24621991459184 37.0 35.0 37.0 32.0 37.0 7 35.1279293436633 37.0 35.0 37.0 32.0 37.0 8 35.13449761008709 37.0 35.0 37.0 32.0 37.0 9 36.627797424487305 39.0 35.0 39.0 32.0 39.0 10-11 36.570000240406344 38.0 35.0 39.0 32.0 39.0 12-13 36.45298001918991 38.0 35.0 39.0 32.0 39.0 14-15 37.507398276925585 39.0 36.0 41.0 32.0 41.0 16-17 37.46750847432354 39.0 36.0 41.0 32.0 41.0 18-19 37.49489151739918 39.0 36.0 41.0 32.0 41.0 20-21 37.47811891471448 39.0 36.0 41.0 32.0 41.0 22-23 37.37190815382232 39.0 36.0 41.0 32.0 41.0 24-25 37.246410870261826 39.0 36.0 40.5 31.0 41.0 26-27 36.986664142907266 39.0 36.0 40.0 31.0 41.0 28-29 36.81560605373599 39.0 36.0 40.0 30.5 41.0 30-31 36.59525020974692 38.0 35.0 40.0 30.0 41.0 32-33 36.43758336242047 38.0 35.0 40.0 30.0 41.0 34-35 36.52425578260937 38.0 35.0 40.0 30.0 41.0 36-37 36.60038729765927 38.5 35.0 40.0 30.0 41.0 38-39 36.59256404927052 39.0 35.0 40.0 30.0 41.0 40-41 36.48057166802136 39.0 35.0 40.0 30.0 41.0 42-43 36.35249290725214 38.5 35.0 40.0 30.0 41.0 44-45 36.18262863336907 38.0 35.0 40.0 30.0 41.0 46-47 35.94088986255148 38.0 35.0 40.0 29.0 41.0 48-49 35.72064098416878 38.0 35.0 40.0 28.0 41.0 50-51 35.459865682846825 38.0 34.0 40.0 27.5 41.0 52-53 35.253144073694585 38.0 34.0 40.0 27.0 41.0 54-55 35.09926362624665 38.0 34.0 40.0 27.0 41.0 56-57 34.92208902155532 38.0 34.0 40.0 26.0 41.0 58-59 34.715581952239475 38.0 33.5 40.0 26.0 41.0 60-61 34.40351458855672 37.0 33.0 40.0 26.0 41.0 62-63 34.144241979025004 37.0 33.0 40.0 26.0 41.0 64-65 33.82485393793195 36.5 32.5 39.5 25.0 41.0 66-67 33.499833084964166 36.0 32.0 39.0 24.0 41.0 68-69 33.162220265159064 36.0 32.0 39.0 23.0 40.0 70-71 32.804083134338754 35.0 31.0 38.5 22.5 40.0 72-73 32.39134135479016 35.0 31.0 38.0 22.0 40.0 74-75 31.87792439859615 35.0 31.0 37.0 21.0 39.0 76-77 30.490933333495633 33.5 29.5 36.0 20.0 38.5 78-79 31.241266478865697 34.0 30.0 36.0 20.5 39.0 80-81 31.22417069702319 34.0 31.0 36.0 21.0 38.5 82-83 30.969382122228062 34.0 31.0 35.5 20.0 37.0 84-85 30.642144357617518 34.0 30.0 35.0 20.0 37.0 86-87 30.311770659653696 34.0 30.0 35.0 18.5 36.5 88-89 29.977504509140463 34.0 30.0 35.0 18.0 36.0 90-91 29.650475350285735 34.0 29.5 35.0 13.0 36.0 92-93 29.333592251977493 34.0 29.0 35.0 7.0 35.0 94-95 28.96702903104376 34.0 29.0 35.0 2.0 35.0 96-97 28.554864835338392 34.0 29.0 35.0 2.0 35.0 98-99 28.071750642021872 34.0 29.0 35.0 2.0 35.0 100-101 26.938707642456734 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30521370078021803 1101 2 0.3236810965113044 1101 3 0.2787508593989898 1101 4 0.18995964789951358 1101 5 0.3053521675040969 1101 6 0.29983565268850043 1101 7 0.3680329261024866 1101 8 0.3587315563886335 1101 9 0.4621221370985964 1101 10-11 0.4151625684926614 1101 12-13 0.3827153418652216 1101 14-15 0.4661137952414478 1101 16-17 0.5295232497774904 1101 18-19 0.4306588389493129 1101 20-21 0.5245507028551373 1101 22-23 0.5833982458637763 1101 24-25 0.4992535668056348 1101 26-27 0.46004540703501107 1101 28-29 0.5695903969993381 1101 30-31 0.5406933628534603 1101 32-33 0.3971129730225229 1101 34-35 0.46031704272929375 1101 36-37 0.4276265065586884 1101 38-39 0.4676109639371475 1101 40-41 0.4930855704039061 1101 42-43 0.4581855682189939 1101 44-45 0.4412328814741002 1101 46-47 0.3539646279038138 1101 48-49 0.4491182928927131 1101 50-51 0.41886415503460483 1101 52-53 0.34511466115318257 1101 54-55 0.35552417890495747 1101 56-57 0.4399895425824667 1101 58-59 0.3985250885271583 1101 60-61 0.38066290855498863 1101 62-63 -0.025093877324366076 1101 64-65 0.13169634222742133 1101 66-67 0.09102452787219306 1101 68-69 0.12434990519116695 1101 70-71 0.1284681471566742 1101 72-73 0.22179579401799998 1101 74-75 0.12641614379854005 1101 76-77 0.1674407391899777 1101 78-79 0.3289175914918374 1101 80-81 0.15992073740948953 1101 82-83 0.15450861474327127 1101 84-85 0.2785770868508557 1101 86-87 0.2713306327546654 1101 88-89 0.32240433939279356 1101 90-91 0.3219499676898181 1101 92-93 0.21209731341622273 1101 94-95 0.417733788944048 1101 96-97 0.5960763157016835 1101 98-99 0.5531681857084756 1101 100-101 0.8508722590237028 1102 1 0.3305152102166602 1102 2 0.4238450006231176 1102 3 0.4107473073175747 1102 4 0.23350173852406897 1102 5 0.3729051251415001 1102 6 0.38541454981567824 1102 7 0.48960209292610557 1102 8 0.43016905138400574 1102 9 0.5089949120374513 1102 10-11 0.4720340166702002 1102 12-13 0.4384476938455393 1102 14-15 0.6477067969312884 1102 16-17 0.6730020828003873 1102 18-19 0.6619132913234012 1102 20-21 0.6608337697688498 1102 22-23 0.6103809501384063 1102 24-25 0.6425865731774252 1102 26-27 0.6584430612705603 1102 28-29 0.6067301662149234 1102 30-31 0.6083099113591075 1102 32-33 0.861121937713655 1102 34-35 0.7090174606546071 1102 36-37 0.7644651739138695 1102 38-39 0.6750749105719791 1102 40-41 0.7219750909981144 1102 42-43 0.8031580570619923 1102 44-45 0.861746728115449 1102 46-47 0.8434806464525337 1102 48-49 0.8550988789429042 1102 50-51 0.6427409407918319 1102 52-53 0.4996230420423089 1102 54-55 0.7535648235578307 1102 56-57 0.6024652467725176 1102 58-59 0.6494146175881426 1102 60-61 0.5674095177700949 1102 62-63 0.48893840251724896 1102 64-65 0.5214850809331679 1102 66-67 0.4675355203103777 1102 68-69 0.5893930321397036 1102 70-71 0.6098133246044668 1102 72-73 0.48258658339415206 1102 74-75 0.6319610243148084 1102 76-77 0.6481501430634182 1102 78-79 0.6488179844131494 1102 80-81 0.6392786101526333 1102 82-83 0.4637873060468074 1102 84-85 0.596594523538986 1102 86-87 0.46260196643261153 1102 88-89 0.5415712125496412 1102 90-91 0.4640694783499768 1102 92-93 0.6148419259140994 1102 94-95 0.5760103341008147 1102 96-97 0.6392360054322879 1102 98-99 0.6878790900021521 1102 100-101 1.0093936200159987 1103 1 0.3936562432593185 1103 2 0.48176388703887696 1103 3 0.4741698095780329 1103 4 0.337175760043273 1103 5 0.34323850051610094 1103 6 0.3711410672536246 1103 7 0.46115362870529 1103 8 0.4792877836089957 1103 9 0.5819060089769081 1103 10-11 0.49413840986377267 1103 12-13 0.4674357813647916 1103 14-15 0.5791824985614795 1103 16-17 0.578840459604983 1103 18-19 0.6278476998472371 1103 20-21 0.5331205095399483 1103 22-23 0.5074616729165342 1103 24-25 0.6000249457186726 1103 26-27 0.49339724813627583 1103 28-29 0.5523580228743512 1103 30-31 0.5507756136820916 1103 32-33 0.6410698539177204 1103 34-35 0.609534301733305 1103 36-37 0.6390982158597964 1103 38-39 0.7156977844535248 1103 40-41 0.6416422751868112 1103 42-43 0.6053219682495268 1103 44-45 0.6634390517952795 1103 46-47 0.645509107795597 1103 48-49 0.5285409836824968 1103 50-51 0.5304798432306441 1103 52-53 0.6446903139847322 1103 54-55 0.6777062491829895 1103 56-57 0.5685089477774241 1103 58-59 0.7341742007756622 1103 60-61 0.5830314146363591 1103 62-63 0.4103570642194896 1103 64-65 0.5310076490977451 1103 66-67 0.5474695806614989 1103 68-69 0.3976846942913781 1103 70-71 0.5281046936515494 1103 72-73 0.4581680407101274 1103 74-75 0.575145454599447 1103 76-77 0.5099818424127278 1103 78-79 0.6916179147786039 1103 80-81 0.5455967014556009 1103 82-83 0.4780671836179131 1103 84-85 0.39139427093612866 1103 86-87 0.4232735045566365 1103 88-89 0.45278774755609774 1103 90-91 0.48521857265681945 1103 92-93 0.3638741922875397 1103 94-95 0.4890649901115047 1103 96-97 0.6751515309155103 1103 98-99 0.6606482088587207 1103 100-101 0.9039597275338664 1104 1 0.33693701762057415 1104 2 0.3787334803624063 1104 3 0.4057188741195361 1104 4 0.2012435508562831 1104 5 0.23347347674484809 1104 6 0.34159289199624254 1104 7 0.30903644842003786 1104 8 0.4133986704988928 1104 9 0.5189258810568091 1104 10-11 0.44851129353858 1104 12-13 0.377679650434807 1104 14-15 0.5067845097117356 1104 16-17 0.5782436131433073 1104 18-19 0.5249233232012074 1104 20-21 0.424582526846784 1104 22-23 0.536912368952386 1104 24-25 0.4730736511262066 1104 26-27 0.6114112372596452 1104 28-29 0.6211082366445737 1104 30-31 0.6698404972200791 1104 32-33 0.6238528001844301 1104 34-35 0.7944241673145882 1104 36-37 0.6229705351812029 1104 38-39 0.7958009072089354 1104 40-41 0.6531670554310196 1104 42-43 0.6621412383524827 1104 44-45 0.8210860402003775 1104 46-47 0.6972611685405354 1104 48-49 0.6381632089450093 1104 50-51 0.7074242971759901 1104 52-53 0.658949642917257 1104 54-55 0.8487438043357045 1104 56-57 0.8424845608704246 1104 58-59 0.8304537438204349 1104 60-61 0.7395898850676659 1104 62-63 0.534201849936224 1104 64-65 0.6239566520734243 1104 66-67 0.6184695122550323 1104 68-69 0.49429942904528446 1104 70-71 0.6421502523614109 1104 72-73 0.6823950475849756 1104 74-75 0.660781805798262 1104 76-77 0.8174603798823696 1104 78-79 0.892183123247321 1104 80-81 0.8564357068255113 1104 82-83 0.84015460708817 1104 84-85 0.9240044908629343 1104 86-87 1.0342134094716435 1104 88-89 1.030323917477773 1104 90-91 0.9029743273512167 1104 92-93 0.9684018456035446 1104 94-95 1.1208645933539714 1104 96-97 1.1455494855620216 1104 98-99 1.134642633731314 1104 100-101 1.3361466824197983 1105 1 0.20365861690507003 1105 2 0.27548389856801236 1105 3 0.24067258165717575 1105 4 0.1961091453707482 1105 5 0.15406741152133918 1105 6 0.21717962204245111 1105 7 0.28283773036514503 1105 8 0.2873200312819719 1105 9 0.24623180881075513 1105 10-11 0.3023558866783489 1105 12-13 0.3544659266658243 1105 14-15 0.3779838074575679 1105 16-17 0.40205980142692255 1105 18-19 0.4047351720840595 1105 20-21 0.44581909043888146 1105 22-23 0.36734324494820925 1105 24-25 0.5123721422734988 1105 26-27 0.5187236244269471 1105 28-29 0.47791794895223916 1105 30-31 0.35111979231589174 1105 32-33 0.5417582111853108 1105 34-35 0.5029228505880141 1105 36-37 0.5527687199204294 1105 38-39 0.5995883593844269 1105 40-41 0.484179278628595 1105 42-43 0.5226945119500428 1105 44-45 0.4881720166483703 1105 46-47 0.42248559174804967 1105 48-49 0.42398231224593275 1105 50-51 0.3761462442750485 1105 52-53 0.396506576849049 1105 54-55 0.4414666126898794 1105 56-57 0.4650709762272598 1105 58-59 0.5517565578058949 1105 60-61 0.4937268080681463 1105 62-63 0.36703802927603846 1105 64-65 0.3578930020080051 1105 66-67 0.47299814070625956 1105 68-69 0.35619675749817503 1105 70-71 0.41039602475817816 1105 72-73 0.35969310810471455 1105 74-75 0.4415609104241973 1105 76-77 0.368134891699782 1105 78-79 0.45601661944620986 1105 80-81 0.3880942854233176 1105 82-83 0.3845158704831739 1105 84-85 0.3837167985297576 1105 86-87 0.5103570568870488 1105 88-89 0.5085730852072068 1105 90-91 0.49883906061057104 1105 92-93 0.4459595248522632 1105 94-95 0.6150246211791348 1105 96-97 0.5577391438756187 1105 98-99 0.6497502078075392 1105 100-101 0.7332589933991471 1106 1 0.19951634347972202 1106 2 0.2191789364507386 1106 3 0.24407848024397438 1106 4 0.12685411454825157 1106 5 0.1709731855644563 1106 6 0.2716367188054747 1106 7 0.1660306015176971 1106 8 0.23393021615098775 1106 9 0.3319000475321232 1106 10-11 0.2775157727250033 1106 12-13 0.29738582445752826 1106 14-15 0.3907012021686711 1106 16-17 0.33654009135801743 1106 18-19 0.352937206682455 1106 20-21 0.2500538231153442 1106 22-23 0.2656098608076576 1106 24-25 0.31195413738083033 1106 26-27 0.3311414544635909 1106 28-29 0.4447382744813879 1106 30-31 0.29162428496121606 1106 32-33 0.32348682802460615 1106 34-35 0.32993794264312015 1106 36-37 0.2675295383126439 1106 38-39 0.2719000716043922 1106 40-41 0.23239615949390924 1106 42-43 0.2296199655447353 1106 44-45 0.3271484160327347 1106 46-47 0.25012496932655637 1106 48-49 0.28614940533037725 1106 50-51 0.1493716617438281 1106 52-53 0.2130470502470061 1106 54-55 0.25187599025189655 1106 56-57 0.25394658595519815 1106 58-59 0.2846741956658505 1106 60-61 0.28596418997846484 1106 62-63 0.11105250933503186 1106 64-65 0.1576078712041422 1106 66-67 0.11429822829923353 1106 68-69 0.05573008984175942 1106 70-71 0.09041822817513179 1106 72-73 0.21022831602667935 1106 74-75 0.48223514538781487 1106 76-77 0.39466203644924036 1106 78-79 0.3282789265890784 1106 80-81 0.2662115478376812 1106 82-83 0.2939397858396582 1106 84-85 0.4315610380957189 1106 86-87 0.40783055217665876 1106 88-89 0.4087853868239648 1106 90-91 0.4694870011859109 1106 92-93 0.5494656357406811 1106 94-95 0.6020453548156652 1106 96-97 0.711166322111648 1106 98-99 0.7895951962851804 1106 100-101 0.9247948067188858 1107 1 0.19333542831347117 1107 2 0.2459253585550627 1107 3 0.18588828160515902 1107 4 -0.003373478516579098 1107 5 0.14785334552158957 1107 6 0.12241446229359809 1107 7 0.21490476062135855 1107 8 0.19876289494568766 1107 9 0.272403478712377 1107 10-11 0.22390754957383763 1107 12-13 0.2326119302701457 1107 14-15 0.229438560319835 1107 16-17 0.23608826364564806 1107 18-19 0.28156932184712957 1107 20-21 0.3114179475733394 1107 22-23 0.3011565420695703 1107 24-25 0.222275085258417 1107 26-27 0.2630070511618072 1107 28-29 0.2626065638197801 1107 30-31 0.37617252205464524 1107 32-33 0.4050868111586894 1107 34-35 0.4536039889787773 1107 36-37 0.4215436098677614 1107 38-39 0.43783275100614105 1107 40-41 0.44180959641152384 1107 42-43 0.4149835991192603 1107 44-45 0.5481343239694496 1107 46-47 0.48823322717753825 1107 48-49 0.45616382503212805 1107 50-51 0.4593160058623269 1107 52-53 0.42840543537166553 1107 54-55 0.49021836932713114 1107 56-57 0.6129225098603825 1107 58-59 0.4681329897561497 1107 60-61 0.33986494161096203 1107 62-63 0.34288727815795284 1107 64-65 0.3138013865876488 1107 66-67 0.26645508033183063 1107 68-69 0.33191282449250537 1107 70-71 0.3760460397421639 1107 72-73 0.38250183327397025 1107 74-75 0.4400782310103999 1107 76-77 0.49961909809169924 1107 78-79 0.45352221496359135 1107 80-81 0.47105937478420046 1107 82-83 0.5529856097782009 1107 84-85 0.612628046805785 1107 86-87 0.5752680797909058 1107 88-89 0.6029836512424787 1107 90-91 0.6601637263697597 1107 92-93 0.5342053862924772 1107 94-95 0.6231236069227144 1107 96-97 0.7347729439852024 1107 98-99 0.9616783157183448 1107 100-101 1.2002454408559906 1108 1 0.14804332960133948 1108 2 0.17106600579642262 1108 3 0.16311888629561722 1108 4 0.05489464695023116 1108 5 0.12240278802302385 1108 6 0.18008114948412413 1108 7 0.16500972028062222 1108 8 0.1431760127898727 1108 9 0.14416335573796601 1108 10-11 0.21433949728716328 1108 12-13 0.2554731556483105 1108 14-15 0.2183865032979071 1108 16-17 0.22891996143840743 1108 18-19 0.31714552348455527 1108 20-21 0.2689854793814632 1108 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-0.7841338415755281 2316 88-89 -0.8254567637248407 2316 90-91 -0.7140958081179996 2316 92-93 -0.6740760127478609 2316 94-95 -1.2775637006969305 2316 96-97 -1.013633363326381 2316 98-99 -1.2346651290537736 2316 100-101 -1.6227791940506435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 10.0 4 42.0 5 69.0 6 142.0 7 467.0 8 1024.0 9 1940.0 10 3306.0 11 5456.0 12 7936.0 13 10184.0 14 12874.0 15 16358.0 16 19985.0 17 23615.0 18 20252.0 19 15781.0 20 15320.0 21 15995.0 22 17634.0 23 20957.0 24 26038.0 25 33853.0 26 43790.0 27 56946.0 28 72353.0 29 91007.0 30 112032.0 31 136181.0 32 163709.0 33 200100.0 34 248645.0 35 310770.0 36 409650.0 37 555974.0 38 517552.0 39 98137.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.05321434614462 22.445918326356164 14.642618464763373 22.858248862735834 2 14.897723544674852 22.036137457705625 40.94725399266807 22.118885004951462 3 17.996347578345844 27.607675989238107 30.938494643551095 23.457481788864953 4 12.158324983470473 18.373092093170143 39.97295528446487 29.49562763889452 5 11.893523860997094 39.14788413502973 36.43284666734834 12.52574533662484 6 28.59301741370973 40.4341452711719 18.112534383578776 12.860302931539591 7 25.447384165190247 33.59481842012208 23.944083293823503 17.013714120864172 8 24.164243810075632 37.7418165064244 20.731020009945023 17.362919673554945 9 25.103686646462393 16.47675072875074 22.04553539557342 36.374027229213446 10-11 23.64787409280841 27.839723830932872 29.263081528485262 19.249320547773458 12-13 24.129858376319916 25.220451093590192 29.783561257818153 20.866129272271746 14-15 21.50212577025127 26.870931921488765 27.031015156859052 24.595927151400915 16-17 20.510784354598748 30.081695552452253 28.05820450545829 21.349315587490715 18-19 20.439773190310834 28.893875207198317 30.326210103578617 20.34014149891224 20-21 20.980717889853118 28.14866119534293 30.285980080356577 20.584640834447367 22-23 21.053732711784022 27.92862154241386 30.02816873977413 20.989477006027986 24-25 20.88941136157983 28.25479023889685 30.187699125722155 20.668099273801168 26-27 20.755560465257116 28.36968592010191 30.226070454420267 20.648683160220706 28-29 20.67609700445498 28.2764672309532 30.252656913757708 20.79477885083411 30-31 20.99889466847967 28.167846052152655 30.07078443235505 20.762474847012623 32-33 21.03181874352823 28.373234853848306 29.864306624841497 20.73063977778197 34-35 21.040832329859207 28.22077151118562 30.131022043154992 20.607374115800177 36-37 21.103661988889765 28.052982446049906 30.238983076894755 20.604372488165573 38-39 21.01314539789349 28.32742408690198 30.159599865250314 20.499830649954216 40-41 20.872006372406933 28.096989007293725 30.390218629247794 20.640785991051548 42-43 20.82765282735883 28.18663640648009 29.92393917264239 21.061771593518692 44-45 20.737724225789155 28.606009625480315 29.790462898320136 20.865803250410394 46-47 21.031041634298504 28.327532298799866 29.565630768729772 21.075795298171858 48-49 21.11016291203164 28.459647551967414 29.300031256077357 21.130158279923585 50-51 21.19153679416436 28.59890750234267 28.9152418713226 21.29431383217037 52-53 20.97066052473036 28.7756570935023 28.9390307713751 21.31465161039224 54-55 21.023882817507737 28.80224937011915 29.015730129153727 21.158137683219387 56-57 21.057081229956896 28.760585990847222 28.929885761457143 21.25244701773874 58-59 20.927427436491378 28.592260247507355 29.223302197202667 21.2570101187986 60-61 21.1172853461414 28.958824507982396 29.010696816487503 20.9131933293887 62-63 20.982811070633197 28.61396545986357 29.201184993217584 21.202038476285647 64-65 20.692909216007386 28.56642139109275 29.158722016796368 21.5819473761035 66-67 20.894283841947455 28.519560094648966 28.961198317405962 21.624957745997616 68-69 20.786359178207146 28.608797253165463 29.019175064941404 21.585668503685987 70-71 20.94049780204713 28.362323413783862 29.124267190175424 21.57291159399358 72-73 21.053964677648914 28.590493807912942 28.950523501523467 21.405018012914677 74-75 20.91979377193439 28.900260905080195 28.80878164299867 21.371163679986747 76-77 20.761152502512758 28.85481315319535 28.98135069332631 21.40268365096558 78-79 20.67536223812743 28.713934628158793 29.06080798240861 21.549895151305165 80-81 20.70248927299839 28.883615836401816 28.883904932899178 21.529989957700618 82-83 20.939133679011277 28.66036152853395 29.07749696220721 21.323007830247562 84-85 20.912384669622348 28.81343037634548 28.67536410149043 21.59882085254175 86-87 20.6065208546415 28.90583466190804 29.019647284336493 21.467997199113967 88-89 20.82281173598596 28.672973234517045 28.786162189928604 21.71805283956839 90-91 20.70171925447541 28.540269472513017 29.136422551988023 21.62158872102355 92-93 20.821463051770255 28.776453987745 28.76803826248297 21.634044698001777 94-95 20.352597241248557 28.542698596522143 28.968944303518956 22.135759858710344 96-97 20.57680211289915 28.508679434989975 28.93027329348672 21.98424515862416 98-99 20.348723092368072 28.792645878720176 28.776897741793245 22.08173328711851 100-101 20.364684247572278 28.618549083823602 28.747212123296194 22.269554545307923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 205.0 1 151.5 2 159.0 3 226.5 4 205.5 5 203.5 6 341.5 7 791.0 8 1364.0 9 1677.0 10 1640.0 11 1553.0 12 1667.5 13 1900.5 14 2218.5 15 2577.5 16 2999.0 17 3448.0 18 3828.0 19 4237.5 20 4823.0 21 5487.5 22 6480.5 23 7747.5 24 9509.0 25 12055.5 26 15950.5 27 20564.0 28 26012.0 29 35547.0 30 48126.0 31 60607.5 32 75029.0 33 89393.0 34 101240.0 35 110871.5 36 123400.5 37 137049.0 38 140497.5 39 138048.0 40 132515.5 41 125556.5 42 122621.0 43 125073.0 44 128481.0 45 132860.5 46 134159.5 47 130364.0 48 129079.5 49 125664.5 50 118044.0 51 109519.0 52 101301.0 53 92885.5 54 83678.5 55 72111.0 56 58356.0 57 45208.0 58 34794.0 59 26786.5 60 19677.0 61 13775.0 62 9462.5 63 6160.0 64 3682.5 65 2096.0 66 1133.5 67 594.0 68 322.5 69 168.0 70 90.0 71 52.5 72 31.0 73 22.5 74 17.5 75 14.0 76 9.0 77 2.5 78 1.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004473383822722538 2 0.0062383924058375525 3 0.0019475956779200164 4 0.17071893364267646 5 0.0021606139551925184 6 4.260365545450036E-4 7 3.0431182467500257E-5 8 0.0011259537512975095 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.1082096331125347E-4 24-25 5.93408058116255E-4 26-27 0.0027996687870100235 28-29 1.9780268603875167E-4 30-31 0.006420979500642554 32-33 0.020723635260367676 34-35 0.013222348782128862 36-37 0.0010194446126612587 38-39 3.043118246750026E-4 40-41 0.0018410865392837657 42-43 0.011487771381481347 44-45 0.007333914974667562 46-47 0.010300955265248837 48-49 0.010407464403885087 50-51 0.18829294151765785 52-53 0.11413214984435972 54-55 0.017071893364267645 56-57 0.006557919821746305 58-59 0.0177870261522539 60-61 0.12187688578233853 62-63 0.23651115013741203 64-65 0.2420344097552633 66-67 0.25279183275752465 68-69 0.2479532747451921 70-71 0.23467006359812825 72-73 0.16014409773522012 74-75 0.26954419870588353 76-77 0.20811885689523427 78-79 0.005401534887981296 80-81 9.129354740250076E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.521559123375013E-4 90-91 0.006147098858435052 92-93 0.01786310410842265 94-95 0.0026627284659062724 96-97 6.0862364935000514E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3286103.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.537928077114856 #Duplication Level Percentage of deduplicated Percentage of total 1 63.17241777461275 22.450168393316396 2 16.743344114303582 11.900475178090101 3 6.993246764007348 7.45576501574428 4 3.762178195949697 5.348000725638002 5 2.2801821227447148 4.051647414041238 6 1.4870575290145254 3.1708166107590205 7 1.0080233386184871 2.507614263551389 8 0.7397820176271223 2.1032256108140466 9 0.5540230457530517 1.7719948037732456 >10 2.858415948498688 18.281773179287075 >50 0.2290795182958208 5.650444844998949 >100 0.15962177047776008 10.743218239451814 >500 0.009363515309750785 2.2455189832711713 >1k 0.0030925371663684765 1.873865824305514 >5k 1.7180762035380425E-4 0.44547091295785435 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7944 0.24174531352182207 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6648 0.2023065010439417 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3973 0.12090308794337852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3924 0.11941196000247101 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3655 0.11122597191871346 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3454 0.10510930424274588 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.0862364935000514E-5 0.0 5 0.0 0.0 0.0 6.0862364935000514E-5 0.0 6 0.0 0.0 0.0 6.0862364935000514E-5 0.0 7 0.0 0.0 0.0 6.0862364935000514E-5 0.0 8 0.0 0.0 0.0 6.0862364935000514E-5 0.0 9 0.0 0.0 0.0 1.8258709480500156E-4 0.0 10-11 0.0 0.0 0.0 1.8258709480500156E-4 0.0 12-13 0.0 0.0 0.0 1.8258709480500156E-4 1.5215591233750128E-5 14-15 1.5215591233750128E-5 0.0 0.0 1.8258709480500156E-4 3.0431182467500257E-5 16-17 3.0431182467500257E-5 0.0 0.0 1.8258709480500156E-4 3.0431182467500257E-5 18-19 3.0431182467500257E-5 0.0 0.0 1.8258709480500156E-4 3.0431182467500257E-5 20-21 3.0431182467500257E-5 0.0 0.0 1.8258709480500156E-4 3.0431182467500257E-5 22-23 3.0431182467500257E-5 0.0 0.0 2.130182772725018E-4 3.0431182467500257E-5 24-25 3.0431182467500257E-5 0.0 0.0 3.043118246750026E-4 3.0431182467500257E-5 26-27 3.0431182467500257E-5 0.0 0.0 3.9560537207750335E-4 3.0431182467500257E-5 28-29 3.0431182467500257E-5 0.0 0.0 7.303483792200061E-4 3.0431182467500257E-5 30-31 3.0431182467500257E-5 0.0 0.0 0.0031344117941525264 3.0431182467500257E-5 32-33 3.0431182467500257E-5 0.0 0.0 0.010240092900313837 3.0431182467500257E-5 34-35 3.0431182467500257E-5 0.0 0.0 0.020723635260367676 3.0431182467500257E-5 36-37 3.0431182467500257E-5 0.0 0.0 0.03936273452171159 3.0431182467500257E-5 38-39 3.0431182467500257E-5 0.0 0.0 0.07101116428791185 3.0431182467500257E-5 40-41 3.0431182467500257E-5 0.0 0.0 0.10891320205118343 3.0431182467500257E-5 42-43 3.0431182467500257E-5 0.0 0.0 0.14775860647094752 3.0431182467500257E-5 44-45 3.0431182467500257E-5 0.0 0.0 0.19664630110498665 3.0431182467500257E-5 46-47 3.0431182467500257E-5 0.0 0.0 0.2578281934558959 3.0431182467500257E-5 48-49 3.0431182467500257E-5 0.0 0.0 0.326207060460369 3.0431182467500257E-5 50-51 3.0431182467500257E-5 0.0 0.0 0.4012199252427572 3.0431182467500257E-5 52-53 3.0431182467500257E-5 0.0 0.0 0.4757458911056653 3.0431182467500257E-5 54-55 3.0431182467500257E-5 0.0 0.0 0.5624595455468073 3.0431182467500257E-5 56-57 3.0431182467500257E-5 0.0 0.0 0.6666406987242943 3.0431182467500257E-5 58-59 3.0431182467500257E-5 0.0 0.0 0.7867677915147516 3.0431182467500257E-5 60-61 3.0431182467500257E-5 0.0 0.0 0.9204976228681816 3.0431182467500257E-5 62-63 3.0431182467500257E-5 0.0 0.0 1.0559924628047264 3.0431182467500257E-5 64-65 3.0431182467500257E-5 0.0 0.0 1.2021230010136628 3.0431182467500257E-5 66-67 3.0431182467500257E-5 0.0 0.0 1.3705443803800428 3.0431182467500257E-5 68-69 3.0431182467500257E-5 0.0 0.0 1.5649844207561356 3.0431182467500257E-5 70-71 3.0431182467500257E-5 0.0 0.0 1.7818522426107763 3.0431182467500257E-5 72-73 3.0431182467500257E-5 0.0 0.0 2.0079407127530695 3.0431182467500257E-5 74-75 3.0431182467500257E-5 0.0 0.0 2.232629348501858 3.0431182467500257E-5 76-77 3.0431182467500257E-5 0.0 0.0 2.486455841463277 3.0431182467500257E-5 78-79 3.0431182467500257E-5 0.0 0.0 2.774639139430505 3.0431182467500257E-5 80-81 3.0431182467500257E-5 0.0 0.0 3.102398190196716 3.0431182467500257E-5 82-83 3.0431182467500257E-5 0.0 0.0 3.4429687687817454 3.0431182467500257E-5 84-85 3.0431182467500257E-5 0.0 0.0 3.778929023222948 3.0431182467500257E-5 86-87 3.0431182467500257E-5 0.0 0.0 4.138275641390425 3.0431182467500257E-5 88-89 3.0431182467500257E-5 0.0 0.0 4.5373653838604575 6.0862364935000514E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4785 0.0 59.894012 1 GTATCAA 9005 0.0 39.531757 1 CAACGCA 11160 0.0 31.455412 5 ATCAACG 11255 0.0 31.287756 3 TATCAAC 11550 0.0 30.573719 2 TCAACGC 11740 0.0 30.15666 4 AACGCAG 12080 0.0 29.334692 6 ACGCAGA 13620 0.0 25.599892 7 CGCAGAG 13570 0.0 25.58934 8 GTACATG 10935 0.0 25.209093 1 ACATGGG 10980 0.0 23.371853 3 TACATGG 11780 0.0 22.956644 2 GCAGAGT 15200 0.0 22.127405 9 GAGTACT 9260 0.0 21.311312 12-13 GTGGTAT 2585 0.0 21.143911 1 CAGAGTA 14940 0.0 20.258064 10-11 CATGGGT 1875 0.0 20.02262 4 AGAGTAC 14105 0.0 19.99432 10-11 GAGTACA 8510 0.0 19.268042 1 GTACTTT 10380 0.0 19.10323 14-15 >>END_MODULE