FastQCFastQC Report
Sat 18 Jun 2016
SRR1931040_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931040_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790990
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN810574.525820914689641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.058.37691
GTATCAA27000.042.9046481
ATCAACG32700.035.1328163
TATCAAC32450.034.2727472
TCAACGC33850.034.0809144
CAACGCA35250.032.7218365
AACGCAG38600.030.5527156
GTACATG26550.026.4493921
ACGCAGA44050.026.1069157
CGCAGAG45050.025.5939228
TACATGG26200.025.0387422
ACATGGG26750.024.8642863
GCAGAGT48100.022.9899319
CATGGGG16500.021.6774834
GAGTACT33350.020.73128712-13
CAGAGTA44650.020.71604310-11
GTGTAGC6200.019.8417171
GTACTTT35500.019.62276814-15
GTGGTAT7450.019.2646621
GTATTAC3504.7475623E-1019.0385991
Produced by FastQC (version 0.11.5)