##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931040_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1790990 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.487199816861065 28.0 2.0 31.0 2.0 33.0 2 19.278539243658535 27.0 2.0 31.0 2.0 33.0 3 19.876268432542894 27.0 2.0 31.0 2.0 33.0 4 21.41585491822958 28.0 2.0 35.0 2.0 37.0 5 20.798663309119537 28.0 2.0 35.0 2.0 37.0 6 21.67450013679585 29.0 2.0 35.0 2.0 37.0 7 21.807577373408 29.0 2.0 35.0 2.0 37.0 8 22.17233820401007 30.0 2.0 35.0 2.0 37.0 9 23.34004209962088 31.0 2.0 37.0 2.0 39.0 10-11 23.630916699702396 32.0 2.0 37.0 2.0 39.0 12-13 23.486610478003787 32.0 2.0 37.0 2.0 39.0 14-15 23.43973863617329 31.5 2.0 38.0 2.0 40.0 16-17 22.373667077984802 29.5 2.0 37.0 2.0 40.0 18-19 21.4032448534051 27.0 2.0 37.0 2.0 39.5 20-21 21.08267773689412 27.0 2.0 37.0 2.0 39.5 22-23 20.73291084819011 26.0 2.0 37.0 2.0 39.0 24-25 20.55018760573761 25.5 2.0 36.5 2.0 39.0 26-27 20.24902651606095 25.0 2.0 36.0 2.0 39.0 28-29 20.027942646245933 24.0 2.0 36.0 2.0 39.0 30-31 19.949786430968345 24.0 2.0 36.0 2.0 39.0 32-33 19.781990965890373 24.0 2.0 36.0 2.0 39.0 34-35 19.440635905281436 23.5 2.0 35.5 2.0 39.0 36-37 19.01247969000385 20.0 2.0 35.0 2.0 38.5 38-39 18.848983243904208 19.0 2.0 35.0 2.0 38.0 40-41 18.66383592314865 19.0 2.0 35.0 2.0 38.0 42-43 18.486616899033493 18.5 2.0 35.0 2.0 38.0 44-45 18.08695665525771 15.5 2.0 34.0 2.0 38.0 46-47 17.737812327260343 15.0 2.0 34.0 2.0 38.0 48-49 17.44409097761573 13.0 2.0 33.5 2.0 38.0 50-51 15.338908648289493 7.0 2.0 31.0 2.0 37.5 52-53 17.113060374429786 12.5 2.0 33.5 2.0 37.5 54-55 17.80798887765984 13.5 2.0 34.5 2.0 38.5 56-57 17.743387735274904 11.5 2.0 35.0 2.0 39.0 58-59 17.460423006270275 8.5 2.0 34.0 2.0 38.5 60-61 17.152079296925166 8.0 2.0 34.0 2.0 38.0 62-63 16.706626781835745 6.5 2.0 34.0 2.0 38.0 64-65 16.34267667602834 2.0 2.0 33.0 2.0 38.0 66-67 15.952881088113276 2.0 2.0 32.5 2.0 37.5 68-69 15.502820507093842 2.0 2.0 32.0 2.0 37.0 70-71 15.133000184255636 2.0 2.0 31.5 2.0 36.0 72-73 14.728758954544693 2.0 2.0 31.0 2.0 36.0 74-75 14.274106499757117 2.0 2.0 31.0 2.0 35.5 76-77 13.841923182150655 2.0 2.0 30.0 2.0 35.0 78-79 13.452687061345959 2.0 2.0 30.0 2.0 34.5 80-81 13.013695218845443 2.0 2.0 29.0 2.0 34.0 82-83 12.553960100279735 2.0 2.0 29.0 2.0 34.0 84-85 12.142663833968921 2.0 2.0 28.5 2.0 34.0 86-87 11.664715604218895 2.0 2.0 26.5 2.0 34.0 88-89 11.249230313960435 2.0 2.0 26.0 2.0 33.5 90-91 10.766529405524318 2.0 2.0 25.5 2.0 33.0 92-93 10.236110475212033 2.0 2.0 24.5 2.0 32.5 94-95 9.689238075031128 2.0 2.0 21.5 2.0 32.0 96-97 9.077439851702131 2.0 2.0 13.0 2.0 32.0 98-99 8.43935030346345 2.0 2.0 2.0 2.0 32.0 100-101 7.614148599377998 2.0 2.0 2.0 2.0 30.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.892794903376952 1101 2 9.533336362201165 1101 3 8.835157412986508 1101 4 10.863745441609492 1101 5 11.4076361175014 1101 6 10.68479742859014 1101 7 10.236713128886244 1101 8 10.263466060362894 1101 9 10.247770272548312 1101 10-11 9.530027248417806 1101 12-13 9.61792966342065 1101 14-15 9.928454222254661 1101 16-17 10.882128656859049 1101 18-19 11.614388950801864 1101 20-21 11.802947412488614 1101 22-23 11.860104939430055 1101 24-25 11.768911448759951 1101 26-27 11.813883627393363 1101 28-29 11.87723747214066 1101 30-31 11.324181463668555 1101 32-33 11.224500979838357 1101 34-35 11.114910466550867 1101 36-37 11.52424712037892 1101 38-39 11.308510349018349 1101 40-41 10.937936546108336 1101 42-43 10.760092686505846 1101 44-45 10.705404671998448 1101 46-47 10.519238081179932 1101 48-49 10.554467672414045 1101 50-51 12.102425458147943 1101 52-53 11.765992609153418 1101 54-55 11.50545501292876 1101 56-57 11.457527334807871 1101 58-59 11.126720509029699 1101 60-61 10.626838866956511 1101 62-63 10.75003481615606 1101 64-65 10.537859438135726 1101 66-67 10.33816082790856 1101 68-69 10.2063460984062 1101 70-71 9.957817539461493 1101 72-73 9.662497495687457 1101 74-75 9.526003137506708 1101 76-77 9.317307115002 1101 78-79 9.013188286516295 1101 80-81 8.993222019088892 1101 82-83 8.60280227284472 1101 84-85 8.217857972948329 1101 86-87 8.065093742264514 1101 88-89 7.964363858245461 1101 90-91 7.424803990484277 1101 92-93 7.262698104480082 1101 94-95 6.971737645987703 1101 96-97 6.663077832016604 1101 98-99 6.48711882209888 1101 100-101 5.782080947151304 1102 1 7.651974776029025 1102 2 9.075654705913792 1102 3 8.28553208359423 1102 4 10.075181027999989 1102 5 10.84199861818885 1102 6 10.241620684174315 1102 7 10.06001718394171 1102 8 9.46940672640876 1102 9 9.368599795790562 1102 10-11 8.395191504124082 1102 12-13 8.951739316397084 1102 14-15 9.30391294789921 1102 16-17 9.991196533806391 1102 18-19 10.799963456608584 1102 20-21 11.090715318984554 1102 22-23 11.25097367748128 1102 24-25 10.704140366489014 1102 26-27 11.154481068317143 1102 28-29 10.71640175953944 1102 30-31 11.130654470513207 1102 32-33 10.770631744960575 1102 34-35 10.754324378999407 1102 36-37 10.72874065091418 1102 38-39 10.841551645278912 1102 40-41 10.329546795463063 1102 42-43 9.995670967448678 1102 44-45 9.959374390746149 1102 46-47 9.82311463329685 1102 48-49 9.364527524633775 1102 50-51 11.020990179361133 1102 52-53 10.332309632966602 1102 54-55 10.150848088490203 1102 56-57 9.729854934594972 1102 58-59 9.848541914331108 1102 60-61 9.806052967509359 1102 62-63 9.885205662038224 1102 64-65 9.571142156681155 1102 66-67 9.425424730429885 1102 68-69 9.395983769231115 1102 70-71 8.893505066318559 1102 72-73 8.694578695602821 1102 74-75 8.472626441375338 1102 76-77 7.857640016758042 1102 78-79 7.595902450920212 1102 80-81 7.468379293123691 1102 82-83 7.215404329688013 1102 84-85 6.816391355871106 1102 86-87 6.552444705949567 1102 88-89 6.503742256450545 1102 90-91 6.128679051189399 1102 92-93 5.890921301712771 1102 94-95 5.397125576178446 1102 96-97 5.0402474866335965 1102 98-99 4.69919804589361 1102 100-101 4.1740179089031155 1103 1 8.012460228897133 1103 2 9.211279418197275 1103 3 8.732891747264024 1103 4 10.827684739427486 1103 5 11.185251585019873 1103 6 10.586806551354204 1103 7 10.261460342250963 1103 8 9.619882695031254 1103 9 9.755149360679997 1103 10-11 9.215104999669446 1103 12-13 9.023045868291437 1103 14-15 9.650490947037678 1103 16-17 10.387935654193452 1103 18-19 11.249878135373667 1103 20-21 11.477530646117074 1103 22-23 11.602755994632236 1103 24-25 11.512275741615888 1103 26-27 11.474533684619324 1103 28-29 10.870285955606345 1103 30-31 10.615892417848837 1103 32-33 10.804507880343095 1103 34-35 10.70464856271645 1103 36-37 10.840103812477285 1103 38-39 10.7007794649932 1103 40-41 10.343146427185925 1103 42-43 9.797515403635234 1103 44-45 9.911085160079313 1103 46-47 9.62771837485948 1103 48-49 9.667947122080239 1103 50-51 11.265992462605448 1103 52-53 10.590565825920159 1103 54-55 9.93966231744692 1103 56-57 9.851038819881818 1103 58-59 9.798665505329023 1103 60-61 9.631473056731519 1103 62-63 9.661444021651977 1103 64-65 9.246343720010216 1103 66-67 9.037376266489431 1103 68-69 8.988432054209582 1103 70-71 8.772090083429026 1103 72-73 8.959078221390541 1103 74-75 8.707179877611813 1103 76-77 8.372094247415637 1103 78-79 7.725449296253059 1103 80-81 7.341308427506023 1103 82-83 7.041127156769697 1103 84-85 7.005184231409332 1103 86-87 6.840107144454498 1103 88-89 6.388039081908998 1103 90-91 6.032726864359004 1103 92-93 5.779056196156628 1103 94-95 5.218999703784997 1103 96-97 4.896984074190932 1103 98-99 4.458888430569436 1103 100-101 3.957068101453638 1104 1 7.878514658251483 1104 2 9.290125695715357 1104 3 8.795537660283493 1104 4 10.624150200263355 1104 5 11.34683426891674 1104 6 10.458601332380688 1104 7 10.265043378964553 1104 8 9.903182133179474 1104 9 9.530245515036722 1104 10-11 9.166966904372355 1104 12-13 8.994737636274916 1104 14-15 9.649564360645666 1104 16-17 10.565361460907706 1104 18-19 11.234354789585556 1104 20-21 11.454184120506298 1104 22-23 11.575487332958101 1104 24-25 11.364999834809062 1104 26-27 11.577390084083373 1104 28-29 11.290319278496778 1104 30-31 11.101303770877365 1104 32-33 11.245557692990339 1104 34-35 11.276187758117327 1104 36-37 11.091894003346408 1104 38-39 10.816170445510991 1104 40-41 10.263920488194898 1104 42-43 10.174383105034288 1104 44-45 10.4819974863038 1104 46-47 10.224502698233113 1104 48-49 9.913220085994407 1104 50-51 11.344374414437114 1104 52-53 10.862135878034376 1104 54-55 10.758814690021378 1104 56-57 10.606794953907336 1104 58-59 10.129537381529293 1104 60-61 9.84448276037137 1104 62-63 9.84784806995837 1104 64-65 9.721359517113463 1104 66-67 9.292426324574393 1104 68-69 8.890859055662741 1104 70-71 8.808201287955937 1104 72-73 8.612230473606118 1104 74-75 8.290437396649457 1104 76-77 8.074999114929417 1104 78-79 7.752294341862585 1104 80-81 7.591673044118734 1104 82-83 7.057139608014026 1104 84-85 7.125382929763424 1104 86-87 6.5786963554607265 1104 88-89 6.110041039510813 1104 90-91 5.675404206386494 1104 92-93 5.224061037061656 1104 94-95 4.899130782867273 1104 96-97 4.587385482861846 1104 98-99 4.4073059472707055 1104 100-101 3.760834449464266 1105 1 7.936947862513261 1105 2 8.997189113529735 1105 3 8.549742678401202 1105 4 10.461883846751416 1105 5 10.350246183909853 1105 6 10.297017930756908 1105 7 10.268849249961452 1105 8 9.610163062011107 1105 9 9.679405222525101 1105 10-11 9.010843562294237 1105 12-13 8.527805762787981 1105 14-15 9.411666987749104 1105 16-17 10.351659528873508 1105 18-19 10.7666532085562 1105 20-21 11.202284669456262 1105 22-23 11.590312452048021 1105 24-25 11.613076063731349 1105 26-27 11.42835197673335 1105 28-29 11.12445356088956 1105 30-31 11.171131405881251 1105 32-33 10.832398538462588 1105 34-35 10.830357846417517 1105 36-37 10.680319348399248 1105 38-39 10.722620259071668 1105 40-41 10.419190922635924 1105 42-43 10.092571954173604 1105 44-45 10.089321199525447 1105 46-47 10.488576527579337 1105 48-49 9.958681897719845 1105 50-51 11.163217941654638 1105 52-53 10.246957080319834 1105 54-55 10.449417573551766 1105 56-57 10.4088141419825 1105 58-59 10.262533941738713 1105 60-61 9.930266040195903 1105 62-63 9.92470689518441 1105 64-65 9.678929175503825 1105 66-67 9.410832574849948 1105 68-69 9.266398540721 1105 70-71 9.06987483012672 1105 72-73 8.753554012178675 1105 74-75 8.539059759526458 1105 76-77 8.223982182216817 1105 78-79 7.740120390233555 1105 80-81 7.495475530222896 1105 82-83 6.9991684842779875 1105 84-85 6.745557573162129 1105 86-87 6.579252302759938 1105 88-89 6.27078532906876 1105 90-91 5.978354951077121 1105 92-93 5.926179240360591 1105 94-95 5.653319160966049 1105 96-97 5.303356999700549 1105 98-99 4.8882330722366785 1105 100-101 4.358471680553738 1106 1 7.738640613703545 1106 2 8.852556162558823 1106 3 8.56442091662904 1106 4 10.140631721928148 1106 5 10.935021238209714 1106 6 10.098106268970952 1106 7 9.5537313950719 1106 8 9.052838171844762 1106 9 8.683174977000313 1106 10-11 8.46130186414479 1106 12-13 8.16048174412705 1106 14-15 8.907264898279625 1106 16-17 9.712775552191498 1106 18-19 10.896806054811076 1106 20-21 10.974379940509952 1106 22-23 10.857014119951433 1106 24-25 10.395593016485883 1106 26-27 10.82783567323624 1106 28-29 10.787237374805951 1106 30-31 10.451462967153585 1106 32-33 10.521515635791125 1106 34-35 10.567607567126828 1106 36-37 10.666652225672951 1106 38-39 10.270147200293565 1106 40-41 9.997789429118207 1106 42-43 9.901762472307542 1106 44-45 9.301031158011895 1106 46-47 9.423160316055668 1106 48-49 9.084324209899851 1106 50-51 10.751782916331626 1106 52-53 10.484914299839634 1106 54-55 9.675615110829778 1106 56-57 9.620673581979634 1106 58-59 9.306816711117794 1106 60-61 9.443960661879352 1106 62-63 9.324960421467352 1106 64-65 8.970111825269495 1106 66-67 8.588728687456346 1106 68-69 8.065427559594873 1106 70-71 7.6016729319371645 1106 72-73 7.663051418674463 1106 74-75 7.397051901311647 1106 76-77 7.115843731877538 1106 78-79 6.746328808637378 1106 80-81 6.643818725105325 1106 82-83 6.424186443212314 1106 84-85 6.157158720390415 1106 86-87 5.9917100072485265 1106 88-89 5.570793169797055 1106 90-91 5.372600730833318 1106 92-93 5.084457455912364 1106 94-95 4.7507529018762344 1106 96-97 4.353862712573644 1106 98-99 3.987545767532156 1106 100-101 3.6047340081952086 1107 1 7.255023151268997 1107 2 8.13983447519957 1107 3 8.115014695409418 1107 4 9.901304762324294 1107 5 10.471749951955854 1107 6 9.527632099421716 1107 7 9.462880926692591 1107 8 8.486342144408606 1107 9 5.626814384342762 1107 10-11 7.674176953885183 1107 12-13 7.9456454746721406 1107 14-15 7.919349240582253 1107 16-17 8.95415280434764 1107 18-19 10.143355059062277 1107 20-21 10.242955295385443 1107 22-23 9.783704516571891 1107 24-25 9.954889591261733 1107 26-27 10.02327476942288 1107 28-29 9.912326975742207 1107 30-31 10.03294557048682 1107 32-33 10.077183965339607 1107 34-35 10.049507480719448 1107 36-37 10.492428399884506 1107 38-39 10.106261718796958 1107 40-41 9.427599751257628 1107 42-43 9.437311940112846 1107 44-45 9.191633923382671 1107 46-47 9.138853965364437 1107 48-49 9.277389892336842 1107 50-51 10.542724896204115 1107 52-53 9.754745183476398 1107 54-55 9.705556187580616 1107 56-57 9.11635237635964 1107 58-59 9.09769426199021 1107 60-61 9.040374337339045 1107 62-63 9.294685206439048 1107 64-65 8.928860112043811 1107 66-67 9.014354332094989 1107 68-69 8.537763392550278 1107 70-71 8.26293369869247 1107 72-73 7.964674666889003 1107 74-75 7.775339105154403 1107 76-77 7.744776062461343 1107 78-79 7.45776303164874 1107 80-81 6.93024551507404 1107 82-83 6.793591370273667 1107 84-85 6.545396021044022 1107 86-87 6.100449006205951 1107 88-89 6.117662617676784 1107 90-91 5.82003796276266 1107 92-93 5.277302099463801 1107 94-95 5.185084828928103 1107 96-97 4.8518061444694 1107 98-99 4.689288446473245 1107 100-101 3.72633509952764 1108 1 6.932261102296074 1108 2 8.083990576669734 1108 3 7.9880945304396 1108 4 9.336502307700957 1108 5 10.949414716997836 1108 6 9.438687423638761 1108 7 8.722462104371637 1108 8 5.91439365430746 1108 9 7.191977682765014 1108 10-11 6.187340281086389 1108 12-13 4.920178591499198 1108 14-15 6.9931224270101 1108 16-17 8.305033274285808 1108 18-19 7.840179855008429 1108 20-21 8.020619894395004 1108 22-23 8.918216514074764 1108 24-25 8.55341046375105 1108 26-27 6.982932488048842 1108 28-29 8.82696251243648 1108 30-31 9.06069816668047 1108 32-33 9.610895741908667 1108 34-35 9.30333950903794 1108 36-37 8.81775343602423 1108 38-39 8.490542101070009 1108 40-41 9.093130756152267 1108 42-43 8.396238562637027 1108 44-45 7.84061426293005 1108 46-47 8.486240282964538 1108 48-49 9.111051693146639 1108 50-51 10.383681054789433 1108 52-53 9.932186524221418 1108 54-55 9.305922233728776 1108 56-57 9.205440116746917 1108 58-59 9.114118189254722 1108 60-61 8.933537454232514 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-14.516296842135397 2313 64-65 -14.16063302417583 2313 66-67 -13.776300366954644 2313 68-69 -13.330504822866828 2313 70-71 -12.970434833514497 2313 72-73 -12.562516462994843 2313 74-75 -12.126984020505576 2313 76-77 -11.686322142396103 2313 78-79 -11.297252807844396 2313 80-81 -10.863841074155106 2313 82-83 -10.422881702588 2313 84-85 -10.02139384168037 2313 86-87 -9.556625967394895 2313 88-89 -9.158472545997979 2313 90-91 -8.681281994998693 2313 92-93 -8.16778765766397 2313 94-95 -7.624577261884992 2313 96-97 -7.041277276861116 2313 98-99 -6.41182031804629 2313 100-101 -5.582799008180913 2314 1 -18.232833237326567 2314 2 -17.042319695866954 2314 3 -17.610962073333983 2314 4 -19.14462976777074 2314 5 -18.500794926608034 2314 6 -19.37997085728367 2314 7 -19.517055715334862 2314 8 -19.89063779223342 2314 9 -21.063787516396413 2314 10-11 -21.35327816755093 2314 12-13 -21.239412603469358 2314 14-15 -21.20499078053707 2314 16-17 -20.11870886407897 2314 18-19 -19.139379893419243 2314 20-21 -18.843897925311495 2314 22-23 -18.491374680893788 2314 24-25 -18.296694358051887 2314 26-27 -18.01051614926772 2314 28-29 -17.793058451924107 2314 30-31 -17.720486319692693 2314 32-33 -17.542668199391123 2314 34-35 -17.213956088446338 2314 36-37 -16.813273817644532 2314 38-39 -16.628954754275902 2314 40-41 -16.44146043881629 2314 42-43 -16.26754550444893 2314 44-45 -15.897331229732426 2314 46-47 -15.553067893136092 2314 48-49 -15.24927325444246 2314 50-51 -13.204107205168638 2314 52-53 -14.955392092130783 2314 54-55 -15.615985523084642 2314 56-57 -15.563689862288722 2314 58-59 -15.27110193653144 2314 60-61 -14.948538017465602 2314 62-63 -14.516296842135397 2314 64-65 -14.16063302417583 2314 66-67 -13.776300366954644 2314 68-69 -13.330504822866828 2314 70-71 -12.970434833514497 2314 72-73 -12.562516462994843 2314 74-75 -12.126984020505576 2314 76-77 -11.686322142396103 2314 78-79 -11.297252807844396 2314 80-81 -10.863841074155106 2314 82-83 -10.422881702588 2314 84-85 -10.02139384168037 2314 86-87 -9.556625967394895 2314 88-89 -9.158472545997979 2314 90-91 -8.681281994998693 2314 92-93 -8.16778765766397 2314 94-95 -7.624577261884992 2314 96-97 -7.041277276861116 2314 98-99 -6.41182031804629 2314 100-101 -5.582799008180913 2315 1 -18.232833237326567 2315 2 -17.042319695866954 2315 3 -17.610962073333983 2315 4 -19.14462976777074 2315 5 -18.500794926608034 2315 6 -19.37997085728367 2315 7 -19.517055715334862 2315 8 -19.89063779223342 2315 9 -21.063787516396413 2315 10-11 -21.35327816755093 2315 12-13 -21.239412603469358 2315 14-15 -21.20499078053707 2315 16-17 -20.11870886407897 2315 18-19 -19.139379893419243 2315 20-21 -18.843897925311495 2315 22-23 -18.491374680893788 2315 24-25 -18.296694358051887 2315 26-27 -18.01051614926772 2315 28-29 -17.793058451924107 2315 30-31 -17.720486319692693 2315 32-33 -17.542668199391123 2315 34-35 -17.213956088446338 2315 36-37 -16.813273817644532 2315 38-39 -16.628954754275902 2315 40-41 -16.44146043881629 2315 42-43 -16.26754550444893 2315 44-45 -15.897331229732426 2315 46-47 -15.553067893136092 2315 48-49 -15.24927325444246 2315 50-51 -13.204107205168638 2315 52-53 -14.955392092130783 2315 54-55 -15.615985523084642 2315 56-57 -15.563689862288722 2315 58-59 -15.27110193653144 2315 60-61 -14.948538017465602 2315 62-63 -14.516296842135397 2315 64-65 -14.16063302417583 2315 66-67 -13.776300366954644 2315 68-69 -13.330504822866828 2315 70-71 -12.970434833514497 2315 72-73 -12.562516462994843 2315 74-75 -12.126984020505576 2315 76-77 -11.686322142396103 2315 78-79 -11.297252807844396 2315 80-81 -10.863841074155106 2315 82-83 -10.422881702588 2315 84-85 -10.02139384168037 2315 86-87 -9.556625967394895 2315 88-89 -9.158472545997979 2315 90-91 -8.681281994998693 2315 92-93 -8.16778765766397 2315 94-95 -7.624577261884992 2315 96-97 -7.041277276861116 2315 98-99 -6.41182031804629 2315 100-101 -5.582799008180913 2316 1 -18.232833237326567 2316 2 -17.042319695866954 2316 3 -17.610962073333983 2316 4 -19.14462976777074 2316 5 -18.500794926608034 2316 6 -19.37997085728367 2316 7 -19.517055715334862 2316 8 -19.89063779223342 2316 9 -21.063787516396413 2316 10-11 -21.35327816755093 2316 12-13 -21.239412603469358 2316 14-15 -21.20499078053707 2316 16-17 -20.11870886407897 2316 18-19 -19.139379893419243 2316 20-21 -18.843897925311495 2316 22-23 -18.491374680893788 2316 24-25 -18.296694358051887 2316 26-27 -18.01051614926772 2316 28-29 -17.793058451924107 2316 30-31 -17.720486319692693 2316 32-33 -17.542668199391123 2316 34-35 -17.213956088446338 2316 36-37 -16.813273817644532 2316 38-39 -16.628954754275902 2316 40-41 -16.44146043881629 2316 42-43 -16.26754550444893 2316 44-45 -15.897331229732426 2316 46-47 -15.553067893136092 2316 48-49 -15.24927325444246 2316 50-51 -13.204107205168638 2316 52-53 -14.955392092130783 2316 54-55 -15.615985523084642 2316 56-57 -15.563689862288722 2316 58-59 -15.27110193653144 2316 60-61 -14.948538017465602 2316 62-63 -14.516296842135397 2316 64-65 -14.16063302417583 2316 66-67 -13.776300366954644 2316 68-69 -13.330504822866828 2316 70-71 -12.970434833514497 2316 72-73 -12.562516462994843 2316 74-75 -12.126984020505576 2316 76-77 -11.686322142396103 2316 78-79 -11.297252807844396 2316 80-81 -10.863841074155106 2316 82-83 -10.422881702588 2316 84-85 -10.02139384168037 2316 86-87 -9.556625967394895 2316 88-89 -9.158472545997979 2316 90-91 -8.681281994998693 2316 92-93 -8.16778765766397 2316 94-95 -7.624577261884992 2316 96-97 -7.041277276861116 2316 98-99 -6.41182031804629 2316 100-101 -5.582799008180913 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 491720.0 3 20645.0 4 65944.0 5 37485.0 6 24457.0 7 24161.0 8 23062.0 9 23470.0 10 24938.0 11 26102.0 12 26487.0 13 26626.0 14 27230.0 15 27610.0 16 28494.0 17 28972.0 18 28366.0 19 29189.0 20 30576.0 21 31616.0 22 33798.0 23 35887.0 24 37092.0 25 40019.0 26 42100.0 27 44677.0 28 47689.0 29 51090.0 30 54926.0 31 58339.0 32 61430.0 33 63574.0 34 63885.0 35 55823.0 36 37963.0 37 14034.0 38 1507.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64557817152059 20.071544935606678 13.996491172475581 23.28638572039715 2 15.21601869640134 20.88681151096235 41.219464855122695 22.677704937513614 3 18.78110611827678 26.018038272356364 30.434269805260044 24.766585804106807 4 12.458583261925165 17.565140118696245 39.20284680567457 30.773429813704023 5 12.797414254635159 37.62279426839725 35.74847749632914 13.831313980638452 6 29.579561384217435 38.29480222793718 18.31293451282949 13.812701875015895 7 25.890127784288147 33.15813112067713 23.1775048913793 17.774236203655423 8 24.18479098102374 36.707093340802466 21.025558434283315 18.082557243890484 9 25.280394596516174 17.114290792747955 21.52226905692956 36.08304555380631 10-11 23.78155399458973 27.118971365694943 29.30245761030463 19.797017029410704 12-13 24.55880675563478 24.84876879696755 29.378741820053104 21.213682627344564 14-15 21.81967412684643 26.566813902925922 27.065938194257683 24.54757377596997 16-17 21.293303918995797 29.51525671604524 27.73714763670406 21.45429172825491 18-19 21.065223257243947 28.31824813371752 29.924235590886777 20.69229301815175 20-21 21.644932363290405 27.612363701303266 30.094720630638534 20.6479833047678 22-23 21.309092595197697 27.50514566212235 29.979425210482923 21.20633653219703 24-25 21.200561232186345 27.632180493256286 30.06074884320903 21.106509431348343 26-27 21.15030707373147 27.904436801092096 29.796637780240616 21.148618344935816 28-29 21.207693923950917 28.14739282134393 29.748021806630703 20.89689144807445 30-31 21.35173908540139 27.92527165721335 29.791084047163004 20.931905210222258 32-33 21.058848858632796 28.058675476752537 29.76402916626179 21.11844649835287 34-35 21.324157756760105 28.02787716170877 29.76310946025307 20.884855621278053 36-37 21.36824996965149 27.93450753068292 29.57556933416531 21.12167316550028 38-39 21.323047362219192 28.004539932938794 29.644321579357637 21.028091125484384 40-41 21.477850682956177 27.953050155837605 29.627960486930238 20.941138674275976 42-43 21.373565910283688 28.040533550033953 29.350029815146094 21.235870724536266 44-45 21.36220060245507 28.236458439620666 29.32121768680322 21.08012327112105 46-47 21.541071979476186 28.18734163904411 28.910321983535486 21.361264397944215 48-49 21.408710321851686 28.363659226292686 28.861077282419124 21.3665531694365 50-51 21.586663059544954 28.555975079493905 28.612787246472276 21.24457461448887 52-53 21.264184553329248 28.722747592692787 28.670404276621486 21.342663577356483 54-55 21.1542495233155 28.669212145486995 28.777444241501666 21.399094089695843 56-57 21.181361995333894 28.72817500369581 28.741945190902822 21.34851781006747 58-59 21.195401512834337 28.64242945451964 28.766114235233736 21.39605479741228 60-61 21.0928593540932 28.785675707825103 28.849289644375194 21.272175293706503 62-63 21.159049023221527 28.760044231478073 28.76866936970144 21.31223737559897 64-65 20.98299032181782 28.578732586973832 28.841460195646373 21.59681689556198 66-67 20.918681923436136 28.738055322410748 28.63432307245461 21.7089396816985 68-69 21.08195594470631 28.67311213987596 28.61304076224641 21.631891153171317 70-71 21.091484398974764 28.505219980907842 28.635344491992104 21.767951128125294 72-73 20.99276309474233 28.65997082719552 28.598767120618213 21.74849895744394 74-75 21.032349766316134 28.936507488375057 28.294203615346834 21.73693912996197 76-77 21.089776129174595 28.641869458609637 28.379954404323517 21.88840000789225 78-79 20.980258056368864 28.795271071280855 28.316993452459922 21.907477419890363 80-81 21.017016167875603 28.867170370157364 28.067726792376874 22.048086669590163 82-83 21.165881563072855 28.59884181013785 28.283176730641795 21.952099896147498 84-85 21.105294027589707 28.765901342294548 28.11076289392021 22.018041736195535 86-87 21.086195001684647 28.6837484334583 28.16897792847478 22.061078636382273 88-89 21.07805068406879 28.65505518247396 28.05656743621113 22.210326697246128 90-91 21.062042385914502 28.702852475979938 28.063030126809966 22.172075011295593 92-93 21.038450617769957 28.653403639750035 28.046659881130807 22.2614858613492 94-95 20.944465658593753 28.53876352988585 28.1159782430813 22.400792568439094 96-97 20.936375091554744 28.477153836274077 28.118606354506927 22.467864717664252 98-99 20.844330541438307 28.553359062607058 27.791820351860107 22.810490044094525 100-101 21.09154404209258 28.55367282534338 27.765457452768306 22.58932567979573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 109375.0 1 99274.0 2 65982.0 3 33387.5 4 20652.5 5 16117.5 6 14768.0 7 14928.0 8 15527.0 9 15563.0 10 14455.0 11 12527.0 12 10150.5 13 7763.0 14 5943.5 15 4829.0 16 4421.5 17 4541.0 18 5180.5 19 6076.5 20 7043.5 21 8252.0 22 9464.0 23 10637.0 24 12094.0 25 13778.0 26 15562.0 27 17705.0 28 20015.5 29 22763.5 30 25743.5 31 28597.5 32 31896.0 33 34858.0 34 37101.5 35 39068.5 36 41481.0 37 43918.5 38 45971.0 39 47746.5 40 48938.0 41 49977.0 42 50538.5 43 51197.5 44 51893.0 45 52333.5 46 52460.5 47 52135.0 48 51272.0 49 49749.5 50 47344.5 51 44122.0 52 40547.0 53 37069.0 54 33449.5 55 29270.5 56 24663.5 57 20159.5 58 16372.5 59 12894.0 60 9971.5 61 7541.0 62 5538.0 63 3888.0 64 2550.5 65 1665.5 66 1095.0 67 694.0 68 407.0 69 259.0 70 161.0 71 105.5 72 81.5 73 54.0 74 36.5 75 22.5 76 15.5 77 14.0 78 9.0 79 4.5 80 4.0 81 3.5 82 3.5 83 3.5 84 2.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.47315730406088 2 29.520823678524167 3 23.30923120732109 4 29.627747781952994 5 32.732231894092095 6 25.357372179632492 7 24.29008537177762 8 20.26197801216087 9 12.044176684403599 10-11 15.715023534469763 12-13 15.80321498165819 14-15 19.96546602716933 16-17 23.2990413123468 18-19 27.26612097219973 20-21 29.340141485993783 22-23 28.953763002585163 24-25 27.45442464782048 26-27 28.91378511326138 28-29 25.859273362777014 30-31 27.76475580544838 32-33 26.73702812411013 34-35 24.795280822338484 36-37 27.788039017526618 38-39 27.9053205210526 40-41 24.92035131407769 42-43 25.793974282380137 44-45 24.485005499751534 46-47 27.787033986789428 48-49 23.18189939642321 50-51 28.79608484692823 52-53 22.343647926565755 54-55 23.468835671890965 56-57 25.59483860881412 58-59 24.882020558462077 60-61 24.42855627334603 62-63 24.259766944539056 64-65 22.79124953238153 66-67 23.297785023925314 68-69 24.29399382464447 70-71 22.76391827983406 72-73 22.638484860328646 74-75 19.90332162658641 76-77 22.17865538054372 78-79 21.298443877408584 80-81 22.331782980362817 82-83 23.198426568545887 84-85 20.13015148046611 86-87 21.6984740283307 88-89 20.518567942869588 90-91 21.652158861858524 92-93 21.636887978157333 94-95 20.67529690283028 96-97 19.575486183619116 98-99 18.99748183965293 100-101 20.665777028347453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1790990.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.79193958830224 #Duplication Level Percentage of deduplicated Percentage of total 1 91.78667767307381 75.99197072920097 2 5.786676460463395 9.581803358634723 3 1.223829584653622 3.0396967511705912 4 0.491440237426332 1.6274916179304715 5 0.23042765112262745 0.9538776085609478 6 0.14169776737321527 0.7038859797696325 7 0.08964636657245906 0.5195397595904481 8 0.060642618702189406 0.401657601925449 9 0.03979537202556933 0.2965262432971454 >10 0.1436089344395627 1.7986047607801772 >50 0.0036206481710670674 0.1976852010645428 >100 0.001669686485337257 0.21202234347435434 >500 6.674987269911213E-5 0.037137081596373404 >1k 1.3349974539822427E-4 0.15860628598451143 >5k 0.0 0.0 >10k+ 6.674987269911213E-5 4.4794946770197015 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 81057 4.525820914689641 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.583504095500254E-5 0.0 0.0 0.0 0.0 9 5.583504095500254E-5 0.0 0.0 0.0 0.0 10-11 5.583504095500254E-5 0.0 0.0 0.0 0.0 12-13 5.583504095500254E-5 0.0 0.0 5.583504095500254E-5 0.0 14-15 5.583504095500254E-5 0.0 0.0 5.583504095500254E-5 0.0 16-17 5.583504095500254E-5 0.0 0.0 5.583504095500254E-5 0.0 18-19 5.583504095500254E-5 0.0 0.0 1.1167008191000508E-4 0.0 20-21 5.583504095500254E-5 0.0 0.0 1.1167008191000508E-4 0.0 22-23 5.583504095500254E-5 0.0 0.0 1.6750512286500764E-4 0.0 24-25 5.583504095500254E-5 0.0 0.0 2.512576842975114E-4 0.0 26-27 5.583504095500254E-5 0.0 0.0 2.791752047750127E-4 0.0 28-29 5.583504095500254E-5 0.0 0.0 4.1876280716251905E-4 0.0 30-31 5.583504095500254E-5 0.0 0.0 0.001563381146740071 0.0 32-33 5.583504095500254E-5 0.0 0.0 0.004243463112580193 0.0 34-35 5.583504095500254E-5 0.0 0.0 0.007342307885582834 0.0 36-37 5.583504095500254E-5 0.0 0.0 0.01340040982920061 0.0 38-39 5.583504095500254E-5 0.0 0.0 0.022138593738658507 0.0 40-41 5.583504095500254E-5 0.0 0.0 0.03151888061909893 0.0 42-43 5.583504095500254E-5 0.0 0.0 0.041150425183836874 0.0 44-45 5.583504095500254E-5 0.0 0.0 0.05228951585435988 0.0 46-47 5.583504095500254E-5 0.0 0.0 0.06775582219889559 0.0 48-49 5.583504095500254E-5 0.0 0.0 0.08403173663727882 0.0 50-51 5.583504095500254E-5 0.0 0.0 0.10566781500734232 0.0 52-53 5.583504095500254E-5 0.0 0.0 0.1297327176589484 0.0 54-55 5.583504095500254E-5 2.791752047750127E-5 0.0 0.15558434162111456 0.0 56-57 5.583504095500254E-5 5.583504095500254E-5 0.0 0.18824784057979105 0.0 58-59 5.583504095500254E-5 5.583504095500254E-5 0.0 0.22512688513057025 0.0 60-61 1.1167008191000508E-4 5.583504095500254E-5 0.0 0.26865029955499475 0.0 62-63 1.1167008191000508E-4 5.583504095500254E-5 0.0 0.31111284820127416 0.0 64-65 1.1167008191000508E-4 5.583504095500254E-5 0.0 0.35480376774856365 0.0 66-67 1.3958760238750635E-4 5.583504095500254E-5 0.0 0.4082379019425011 2.791752047750127E-5 68-69 1.675051228650076E-4 5.583504095500254E-5 0.0 0.4704102200458964 5.583504095500254E-5 70-71 1.675051228650076E-4 5.583504095500254E-5 0.0 0.5398690109939195 5.583504095500254E-5 72-73 1.675051228650076E-4 8.375256143250382E-5 0.0 0.6120358014282603 5.583504095500254E-5 74-75 1.675051228650076E-4 1.1167008191000508E-4 0.0 0.6868826738284413 5.583504095500254E-5 76-77 1.675051228650076E-4 1.1167008191000508E-4 0.0 0.7713610907933601 5.583504095500254E-5 78-79 1.675051228650076E-4 1.1167008191000508E-4 0.0 0.8718641645123647 5.583504095500254E-5 80-81 1.675051228650076E-4 1.1167008191000508E-4 0.0 0.9850138750076773 5.583504095500254E-5 82-83 1.675051228650076E-4 1.1167008191000508E-4 0.0 1.1033004092708503 5.583504095500254E-5 84-85 1.675051228650076E-4 1.1167008191000508E-4 0.0 1.223261994762673 5.583504095500254E-5 86-87 1.675051228650076E-4 1.1167008191000508E-4 0.0 1.3447869614012362 5.583504095500254E-5 88-89 1.675051228650076E-4 1.1167008191000508E-4 0.0 1.4809686262904873 5.583504095500254E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1440 0.0 58.3769 1 GTATCAA 2700 0.0 42.904648 1 ATCAACG 3270 0.0 35.132816 3 TATCAAC 3245 0.0 34.272747 2 TCAACGC 3385 0.0 34.080914 4 CAACGCA 3525 0.0 32.721836 5 AACGCAG 3860 0.0 30.552715 6 GTACATG 2655 0.0 26.449392 1 ACGCAGA 4405 0.0 26.106915 7 CGCAGAG 4505 0.0 25.593922 8 TACATGG 2620 0.0 25.038742 2 ACATGGG 2675 0.0 24.864286 3 GCAGAGT 4810 0.0 22.989931 9 CATGGGG 1650 0.0 21.677483 4 GAGTACT 3335 0.0 20.731287 12-13 CAGAGTA 4465 0.0 20.716043 10-11 GTGTAGC 620 0.0 19.841717 1 GTACTTT 3550 0.0 19.622768 14-15 GTGGTAT 745 0.0 19.264662 1 GTATTAC 350 4.7475623E-10 19.038599 1 >>END_MODULE