FastQCFastQC Report
Sat 18 Jun 2016
SRR1931040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790990
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53680.29972249984645366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.29536736665196345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29580.1651600511448975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27050.058.8777961
GTATCAA51850.041.1691251
TCAACGC63900.033.1777764
ATCAACG64050.033.100083
CAACGCA65350.032.4562645
TATCAAC66850.031.7181222
AACGCAG69300.030.7432426
TGGTATC12200.028.0573522
CGCAGAG76100.027.6843858
ACGCAGA76450.027.5576427
GTGGTAT13200.027.0122871
GCAGAGT87000.023.7788989
TAACGCA2202.1518645E-923.7674874
GTACATG42600.022.3200131
GCGTAGG1100.00286339521.6098292
TAGACCG1553.93983E-521.4290495
AGAGTAC79450.021.11154410-11
TACATGG44200.020.9742452
GAGTACT62850.020.7989212-13
ACATGGG42800.020.546663
Produced by FastQC (version 0.11.5)