FastQCFastQC Report
Sat 18 Jun 2016
SRR1931039_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931039_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431302
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1082474.45222354113146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.051.063611
GTATCAA36900.046.194191
TCAACGC44700.036.8234754
ATCAACG44300.036.702593
TATCAAC46300.035.3739932
CAACGCA46550.035.136185
AACGCAG51050.032.6383256
ACGCAGA58100.028.565917
CGCAGAG58400.028.2073848
GCAGAGT64750.024.206949
GTGGTAT10500.023.8878061
GAGTACT41650.020.9851612-13
GTACATG40100.020.8071561
AACGCCG2301.0551594E-720.3491256
GAGTACA30700.020.3418731
TATACCG1756.376155E-520.1303315
ACGCCGA2051.0391977E-619.9627347
TACATGG39900.019.8826332
AGAGTAC55550.019.8424810-11
CAGAGTA61300.019.66347110-11
Produced by FastQC (version 0.11.5)