FastQCFastQC Report
Sat 18 Jun 2016
SRR1931039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431302
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63660.26183501679347115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57100.23485358873558282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32000.1316167222336016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.058.6117971
GTATCAA62800.041.470851
ATCAACG80150.032.4310533
TCAACGC80800.032.1701664
CAACGCA81550.031.8264435
TATCAAC82000.031.4675712
AACGCAG85850.030.395656
ACGCAGA98850.026.1102647
CGCAGAG99450.025.9527368
GTACATG63550.024.603811
GTGGTAT16250.023.1044541
TACATGG66050.022.8067672
ACATGGG63850.022.4017983
GCAGAGT112750.022.3863939
TGGTATC18600.020.4387512
AGAGTAC104100.020.16747710-11
GAGTACT80100.020.13888512-13
ACCGTGC3655.456968E-1219.497888
CAGAGTA109900.019.44850210-11
GTACTAG7650.019.2584921
Produced by FastQC (version 0.11.5)