##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931039_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2431302 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.353810016197084 31.0 31.0 34.0 30.0 34.0 2 31.510155052724837 31.0 31.0 34.0 30.0 34.0 3 31.57600248755605 31.0 31.0 34.0 30.0 34.0 4 35.343699384116 37.0 35.0 37.0 33.0 37.0 5 35.223275018899336 37.0 35.0 37.0 33.0 37.0 6 35.253049600584376 37.0 35.0 37.0 32.0 37.0 7 35.133280439863086 37.0 35.0 37.0 32.0 37.0 8 35.139778604221114 37.0 35.0 37.0 32.0 37.0 9 36.63901070290733 39.0 35.0 39.0 32.0 39.0 10-11 36.584727647984494 38.5 35.0 39.0 32.0 39.0 12-13 36.473750895610664 38.0 35.0 39.0 32.0 39.0 14-15 37.539740229720536 39.0 36.0 41.0 32.0 41.0 16-17 37.49674824435631 39.0 36.0 41.0 32.0 41.0 18-19 37.523304385880486 39.0 36.0 41.0 32.0 41.0 20-21 37.51219840233751 39.0 36.0 41.0 32.0 41.0 22-23 37.41423299121212 39.0 36.0 41.0 32.0 41.0 24-25 37.289077827435676 39.0 36.0 41.0 31.5 41.0 26-27 37.03665134977062 39.0 36.0 40.0 31.0 41.0 28-29 36.852976923475566 39.0 36.0 40.0 30.5 41.0 30-31 36.62755675765495 38.5 35.0 40.0 30.0 41.0 32-33 36.46013843611365 38.0 35.0 40.0 30.0 41.0 34-35 36.53322623022562 38.0 35.0 40.0 30.0 41.0 36-37 36.59666795815575 39.0 35.0 40.0 30.0 41.0 38-39 36.585389433315974 39.0 35.0 40.0 30.0 41.0 40-41 36.46705592312267 39.0 35.0 40.0 30.0 41.0 42-43 36.32044476580861 39.0 35.0 40.0 30.0 41.0 44-45 36.15318582389189 38.5 35.0 40.0 30.0 41.0 46-47 35.90969488775973 38.0 35.0 40.0 29.0 41.0 48-49 35.68850866737246 38.0 35.0 40.0 28.0 41.0 50-51 35.426708405619706 38.0 34.5 40.0 27.0 41.0 52-53 35.222096843584225 38.0 34.0 40.0 27.0 41.0 54-55 35.073417041568675 38.0 34.0 40.0 26.0 41.0 56-57 34.90412009696862 38.0 34.0 40.0 26.0 41.0 58-59 34.69747711308591 38.0 33.5 40.0 26.0 41.0 60-61 34.39063616942691 37.5 33.0 40.0 25.5 41.0 62-63 34.143935019179025 37.0 33.0 40.0 25.0 41.0 64-65 33.83154972109594 37.0 32.5 40.0 24.0 41.0 66-67 33.513268816461306 36.0 32.0 39.0 23.0 41.0 68-69 33.16926589127965 36.0 32.0 39.0 22.5 40.0 70-71 32.79189915526742 35.5 31.0 39.0 22.0 40.0 72-73 32.37223224428722 35.0 31.0 38.0 22.0 40.0 74-75 31.849150167276626 35.0 31.0 37.5 20.0 39.5 76-77 30.462698792663353 33.5 29.5 36.0 19.5 38.5 78-79 31.197698805002425 34.0 30.0 36.0 20.0 39.0 80-81 31.161688058497052 34.0 31.0 36.0 20.0 38.5 82-83 30.879573372620925 34.0 30.0 36.0 20.0 37.0 84-85 30.530837386717074 34.0 30.0 35.0 18.5 37.0 86-87 30.17978001087483 34.0 30.0 35.0 18.0 36.5 88-89 29.825992616301882 34.0 29.5 35.0 14.0 36.0 90-91 29.483000672067888 34.0 29.0 35.0 8.5 36.0 92-93 29.16210223986983 34.0 29.0 35.0 4.5 35.5 94-95 28.781708730548488 34.0 29.0 35.0 2.0 35.0 96-97 28.349252581538614 34.0 29.0 35.0 2.0 35.0 98-99 27.854515605218932 33.5 29.0 35.0 2.0 35.0 100-101 26.71799430922197 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4230919320595632 1101 2 0.3952790615690205 1101 3 0.3895238913197616 1101 4 0.31937970219973977 1101 5 0.3590338676868612 1101 6 0.4081414739188176 1101 7 0.4484657030350263 1101 8 0.3993723481247571 1101 9 0.5913730110484892 1101 10-11 0.5463480514436441 1101 12-13 0.48123592508176927 1101 14-15 0.5144859402182362 1101 16-17 0.6792739869078801 1101 18-19 0.5706675291465046 1101 20-21 0.49332742241895033 1101 22-23 0.5539224562260472 1101 24-25 0.6199558601083837 1101 26-27 0.5141014836882221 1101 28-29 0.6168246937964312 1101 30-31 0.5135271521336975 1101 32-33 0.5444881394255177 1101 34-35 0.622997839599492 1101 36-37 0.5258704704238184 1101 38-39 0.622770641174192 1101 40-41 0.6350143073065198 1101 42-43 0.5119494575242811 1101 44-45 0.503458868810128 1101 46-47 0.4584410624857469 1101 48-49 0.5136071175479131 1101 50-51 0.5507987389929454 1101 52-53 0.4230830311457936 1101 54-55 0.5124142291412142 1101 56-57 0.6532539575197376 1101 58-59 0.5068677589120725 1101 60-61 0.5519706196946359 1101 62-63 0.2724458381641526 1101 64-65 0.4437720067132531 1101 66-67 0.5749292747135897 1101 68-69 0.42112773223337996 1101 70-71 0.4400611312838123 1101 72-73 0.49738621983761533 1101 74-75 0.4911389401006865 1101 76-77 0.4458958674478666 1101 78-79 0.46584427134288475 1101 80-81 0.38322166472012853 1101 82-83 0.6560917368498203 1101 84-85 0.6774324358426931 1101 86-87 0.5820241382014437 1101 88-89 0.5638315707302901 1101 90-91 0.6744968088168122 1101 92-93 0.5586432011475715 1101 94-95 0.7283782332580238 1101 96-97 0.8880955041830596 1101 98-99 0.9270522403849526 1101 100-101 1.1163776479210128 1102 1 0.33866589971115957 1102 2 0.37583579351134944 1102 3 0.38442723175873184 1102 4 0.2772687359353583 1102 5 0.3756427665336375 1102 6 0.3664249849719923 1102 7 0.4362884831931808 1102 8 0.3908175057619019 1102 9 0.5579653090405401 1102 10-11 0.4106449362400255 1102 12-13 0.42346700109317936 1102 14-15 0.5588554113646893 1102 16-17 0.48247428469802855 1102 18-19 0.48750466017180827 1102 20-21 0.466147209691421 1102 22-23 0.51235691281817 1102 24-25 0.579947327032464 1102 26-27 0.5403062286147104 1102 28-29 0.45138104876923535 1102 30-31 0.49913267323886856 1102 32-33 0.6958813099679517 1102 34-35 0.602179105948963 1102 36-37 0.6416531908662151 1102 38-39 0.5773298825663957 1102 40-41 0.6982603303962946 1102 42-43 0.6560524474920015 1102 44-45 0.6983441494118949 1102 46-47 0.6618902548009302 1102 48-49 0.5659067499631973 1102 50-51 0.49374518960402014 1102 52-53 0.5097298714234881 1102 54-55 0.5991623306895519 1102 56-57 0.5109900741184603 1102 58-59 0.6430581742646595 1102 60-61 0.5753430458720956 1102 62-63 0.2399660490147255 1102 64-65 0.33940846613342757 1102 66-67 0.3781998402546307 1102 68-69 0.46822281924789166 1102 70-71 0.3873309018765596 1102 72-73 0.3632891683229147 1102 74-75 0.4717284005689599 1102 76-77 0.3301354654942479 1102 78-79 0.5479722091224062 1102 80-81 0.4537836568857898 1102 82-83 0.47358430589715184 1102 84-85 0.5258918686376219 1102 86-87 0.5301916167721465 1102 88-89 0.573511958930041 1102 90-91 0.48660202590157553 1102 92-93 0.4753728406527209 1102 94-95 0.5622746549654742 1102 96-97 0.568637804028409 1102 98-99 0.854450354117759 1102 100-101 1.1727046240339192 1103 1 0.26934557514278623 1103 2 0.35046839196412094 1103 3 0.3364024847370679 1103 4 0.2574046493300841 1103 5 0.30364895173965323 1103 6 0.3006047556917366 1103 7 0.4516180948669799 1103 8 0.3780021059623948 1103 9 0.49363048983231295 1103 10-11 0.49405341548812487 1103 12-13 0.4505535397483911 1103 14-15 0.47945344889426167 1103 16-17 0.5105903998556585 1103 18-19 0.5669095665028152 1103 20-21 0.49756610400837076 1103 22-23 0.5058702709728919 1103 24-25 0.6173627104083224 1103 26-27 0.6316365592102642 1103 28-29 0.5265968414966622 1103 30-31 0.5684050438678199 1103 32-33 0.6857036092470068 1103 34-35 0.681262279306722 1103 36-37 0.5188168896565983 1103 38-39 0.5574934527565532 1103 40-41 0.5619445564166199 1103 42-43 0.5423263876634579 1103 44-45 0.5702653748016075 1103 46-47 0.690806325335835 1103 48-49 0.48126781446318034 1103 50-51 0.34497549091589974 1103 52-53 0.323413865033757 1103 54-55 0.4602654107424158 1103 56-57 0.52644890577767 1103 58-59 0.5500256724944776 1103 60-61 0.387123911088203 1103 62-63 0.3511375350910342 1103 64-65 0.4185678699861697 1103 66-67 0.43257898780557014 1103 68-69 0.28295103119345555 1103 70-71 0.3744714991084024 1103 72-73 0.3307335564724809 1103 74-75 0.4902868850075599 1103 76-77 0.460435770261288 1103 78-79 0.34433584407749507 1103 80-81 0.39409060858013234 1103 82-83 0.39128147870641783 1103 84-85 0.21848310120105907 1103 86-87 0.21859598236244793 1103 88-89 0.1729564989208363 1103 90-91 0.2848475624666449 1103 92-93 0.14227997424665872 1103 94-95 0.25347220195491005 1103 96-97 0.3090656864165311 1103 98-99 0.2651655418043646 1103 100-101 0.38528273317677275 1104 1 0.33740589274979627 1104 2 0.3754157911908962 1104 3 0.31030726424510036 1104 4 0.18238069787989986 1104 5 0.205216963076154 1104 6 0.30369148920682676 1104 7 0.3542720079640347 1104 8 0.36780787587444763 1104 9 0.5091615034946528 1104 10-11 0.44397362866373413 1104 12-13 0.4067980139716951 1104 14-15 0.4794842546179723 1104 16-17 0.4798452093992722 1104 18-19 0.5740634256898574 1104 20-21 0.41856744405735213 1104 22-23 0.468962087432395 1104 24-25 0.423045134202674 1104 26-27 0.44809251472679534 1104 28-29 0.5574235130922389 1104 30-31 0.5822525247297605 1104 32-33 0.6017926685942356 1104 34-35 0.5890128453618928 1104 36-37 0.663199773996503 1104 38-39 0.6757415312495354 1104 40-41 0.5197790680688854 1104 42-43 0.5437459154117121 1104 44-45 0.6438761136768889 1104 46-47 0.6058048620639553 1104 48-49 0.6294767696870593 1104 50-51 0.5802865517109979 1104 52-53 0.43944793616417144 1104 54-55 0.5996623887009207 1104 56-57 0.6268144156894095 1104 58-59 0.7828296094659635 1104 60-61 0.6785127871413792 1104 62-63 0.672947833444475 1104 64-65 0.6401962605411384 1104 66-67 0.5501513427491247 1104 68-69 0.5558153473834082 1104 70-71 0.5652239289098588 1104 72-73 0.602993310334746 1104 74-75 0.6607746936424057 1104 76-77 0.7504266825727974 1104 78-79 0.8825400244142081 1104 80-81 0.7830643158949542 1104 82-83 0.6252190663101977 1104 84-85 0.7430597535430614 1104 86-87 0.8313738884974065 1104 88-89 0.8936291418979003 1104 90-91 0.7621455924406142 1104 92-93 0.7342207348399761 1104 94-95 0.8882045772302369 1104 96-97 0.8293130563778668 1104 98-99 0.9470782691891131 1104 100-101 1.0968350335941892 1105 1 0.2752193849924325 1105 2 0.34595496925659575 1105 3 0.36167409493747016 1105 4 0.05722577635641812 1105 5 0.18429992396580985 1105 6 0.1601855526802538 1105 7 0.14137718004054278 1105 8 0.2395027579715645 1105 9 0.34361562896678777 1105 10-11 0.3875484860817622 1105 12-13 0.2991149898794845 1105 14-15 0.3502049143323518 1105 16-17 0.4292383143004841 1105 18-19 0.31907192793956085 1105 20-21 0.3353639769452599 1105 22-23 0.2634535325138785 1105 24-25 0.3742133282646378 1105 26-27 0.41307949800820865 1105 28-29 0.38715715942875306 1105 30-31 0.1784074011085508 1105 32-33 0.40688799912860674 1105 34-35 0.4665497511449246 1105 36-37 0.4939302150601179 1105 38-39 0.3802060400000826 1105 40-41 0.24197911923286597 1105 42-43 0.4346122419883969 1105 44-45 0.3914897654307623 1105 46-47 0.3858155463069579 1105 48-49 0.34734323169780623 1105 50-51 0.3759225651340117 1105 52-53 0.14605516232401783 1105 54-55 0.3019516758662846 1105 56-57 0.42225886514993505 1105 58-59 0.43365982029165906 1105 60-61 0.2843320880006317 1105 62-63 0.2543393432333474 1105 64-65 0.11840898231441344 1105 66-67 0.20891919371145917 1105 68-69 0.11816211878574734 1105 70-71 0.18904572777522333 1105 72-73 0.18431589923216762 1105 74-75 0.32208223431024763 1105 76-77 0.27726324375137423 1105 78-79 0.30205261225824387 1105 80-81 0.2733094515547414 1105 82-83 0.3005726244640776 1105 84-85 0.3123269047627204 1105 86-87 0.5147413813118753 1105 88-89 0.48904575213370904 1105 90-91 0.5648359149241742 1105 92-93 0.6519630489567234 1105 94-95 0.6930398743575275 1105 96-97 0.5500334151276611 1105 98-99 0.6646904161200275 1105 100-101 0.7722265132866042 1106 1 0.19429862898681804 1106 2 0.30049565395871625 1106 3 0.27650088885600965 1106 4 -0.0020947979963139574 1106 5 0.056635862037829554 1106 6 0.09168821744620459 1106 7 0.18549232923275127 1106 8 0.16655006568200292 1106 9 0.34482551185581656 1106 10-11 0.30993323150834584 1106 12-13 0.2763749571475813 1106 14-15 0.3256660645377636 1106 16-17 0.26361182380865245 1106 18-19 0.3675005919816243 1106 20-21 0.29230315850535504 1106 22-23 0.3042980109095481 1106 24-25 0.3915108147970372 1106 26-27 0.38915833618862905 1106 28-29 0.35472976351825736 1106 30-31 0.24283303503353437 1106 32-33 0.32259674920678805 1106 34-35 0.26240843302916517 1106 36-37 0.22624092093037262 1106 38-39 0.13550637345525018 1106 40-41 0.07344071349182002 1106 42-43 0.17255535807638722 1106 44-45 0.2189634742294544 1106 46-47 0.10419851848365624 1106 48-49 0.07450960089602177 1106 50-51 -0.11322672845622606 1106 52-53 -0.2570094741464146 1106 54-55 -0.012081187060786647 1106 56-57 0.1678720577497117 1106 58-59 0.15792946632644345 1106 60-61 0.09094684071646242 1106 62-63 -0.05181734956963879 1106 64-65 -0.033580350914569124 1106 66-67 -0.1930188569646063 1106 68-69 -0.22602229149601527 1106 70-71 -0.14358528654921088 1106 72-73 -0.005083167069649619 1106 74-75 0.16699490130832473 1106 76-77 0.28589961102575145 1106 78-79 0.13755604240888886 1106 80-81 0.1156980831909813 1106 82-83 0.2584846923752231 1106 84-85 0.2430418043007947 1106 86-87 0.2853299923758037 1106 88-89 0.2592113562436644 1106 90-91 0.32441196828411734 1106 92-93 0.2982093291189436 1106 94-95 0.3113038887972728 1106 96-97 0.29343456593186446 1106 98-99 0.35214222756504654 1106 100-101 0.5287946818799263 1107 1 0.14347916416362594 1107 2 0.1205344702414628 1107 3 0.041256696649639935 1107 4 0.0821123800264445 1107 5 0.07686298990794427 1107 6 0.25214350159072296 1107 7 0.14735766327878963 1107 8 0.17445906266495115 1107 9 0.14465169957308177 1107 10-11 0.15332863382369766 1107 12-13 0.10942443296035265 1107 14-15 0.15079488413293518 1107 16-17 0.130345725395145 1107 18-19 0.275134117124324 1107 20-21 0.2197052149551837 1107 22-23 0.2176153382063859 1107 24-25 0.1054264551521058 1107 26-27 0.29336083258024814 1107 28-29 0.2519358969931673 1107 30-31 0.4085579942860065 1107 32-33 0.3079975189554389 1107 34-35 0.29252677725043696 1107 36-37 0.32857903096245167 1107 38-39 0.2637859068945332 1107 40-41 0.18415471306371956 1107 42-43 0.1701855438946822 1107 44-45 0.24401543256233538 1107 46-47 0.14420785484001186 1107 48-49 0.05232506690068561 1107 50-51 0.22148882209283016 1107 52-53 0.32454597537986274 1107 54-55 0.3180989212086658 1107 56-57 0.32815342497733724 1107 58-59 0.3272800738622479 1107 60-61 0.24350762718265884 1107 62-63 0.060156295776771174 1107 64-65 0.0907612760210057 1107 66-67 0.1777184222124859 1107 68-69 0.1321724165268492 1107 70-71 0.04609596193359522 1107 72-73 0.1639350028012032 1107 74-75 0.2554057565938983 1107 76-77 0.2719798301476182 1107 78-79 0.20646675300037387 1107 80-81 0.21331973385160907 1107 82-83 0.20896261636179503 1107 84-85 0.3203094955451249 1107 86-87 0.3579321960309443 1107 88-89 0.41542738751193653 1107 90-91 0.5515057575592941 1107 92-93 0.40176717446846055 1107 94-95 0.39536143997533557 1107 96-97 0.5507423649433996 1107 98-99 0.636232035958983 1107 100-101 0.8042473142959459 1108 1 0.052878756760890155 1108 2 0.10666445360743992 1108 3 0.07445038190722997 1108 4 -0.046728735286137635 1108 5 0.08265663994613703 1108 6 0.07585317478805109 1108 7 0.053364711917254226 1108 8 0.13821193911388008 1108 9 0.1690560039543243 1108 10-11 0.08827002208659707 1108 12-13 0.07696696394984315 1108 14-15 0.12995297400426153 1108 16-17 0.08587737168770815 1108 18-19 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-0.7301336073959668 2316 88-89 -0.7286948969435088 2316 90-91 -0.7451874913761571 2316 92-93 -0.5637513193051156 2316 94-95 -0.9191931729620997 2316 96-97 -0.7331299186912652 2316 98-99 -0.9012077599627375 2316 100-101 -1.207310268216883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 10.0 4 23.0 5 59.0 6 107.0 7 304.0 8 764.0 9 1482.0 10 2655.0 11 4322.0 12 6198.0 13 8087.0 14 10122.0 15 12991.0 16 15635.0 17 18184.0 18 15933.0 19 12549.0 20 11955.0 21 12637.0 22 13859.0 23 16525.0 24 20071.0 25 25442.0 26 32679.0 27 41603.0 28 52814.0 29 66723.0 30 81535.0 31 98640.0 32 119628.0 33 144037.0 34 179210.0 35 224661.0 36 294615.0 37 408203.0 38 399887.0 39 77123.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.14992627082567 21.754363594857672 15.174800869531238 22.920909264785426 2 14.823716201903203 21.862851760370027 41.051142147900286 22.262289889826484 3 18.31884770906949 26.491520244021515 31.44977030438114 23.73986174252785 4 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20.708836091705017 24-25 20.5166493472681 28.227911905717214 30.763591134963185 20.491847612051505 26-27 20.489600216516056 28.39648761932496 30.589638119164857 20.524274044994126 28-29 20.445923035223217 28.502805501926137 30.605327862446913 20.445943600403734 30-31 20.745569582765015 28.216463991497022 30.55445410974568 20.48351231599228 32-33 20.594311652526773 28.42349256159113 30.419190325604966 20.563005460277132 34-35 20.76846280290054 28.341428780631478 30.524834265461564 20.365274151006417 36-37 20.769451450934746 28.198303155439884 30.566482024367847 20.46576336925752 38-39 20.616663643583887 28.347241570637514 30.575438665763443 20.46065612001515 40-41 20.745890496883295 28.367633177104008 30.44834869234202 20.43812763367068 42-43 20.679105470683826 28.385948046942406 30.241731716482146 20.69321476589162 44-45 20.69301037629601 28.637853037162326 30.110159194247256 20.55897739229441 46-47 20.781650386654153 28.663928342030196 29.826636531521277 20.727784739794373 48-49 20.915377752256124 28.6974028928229 29.71280734135154 20.67441201356944 50-51 20.93960044069011 28.871136466612285 29.38777494968884 20.80148814300876 52-53 20.957591060567317 28.89052637387117 29.33400617083402 20.81787639472749 54-55 20.914860602151318 28.896545454246276 29.44101816458539 20.747575779017016 56-57 20.89349551259349 28.974302970174037 29.323542852582968 20.808658664649503 58-59 20.875469146043642 28.914761097783742 29.480811368889327 20.728958387283285 60-61 20.881847680855124 28.97388332836067 29.505412583781133 20.638856407003072 62-63 20.878998097685145 28.876925093981942 29.511751556195797 20.732325252137116 64-65 20.701181486268908 28.794949975982696 29.501038000935953 21.00283053681244 66-67 20.724051329218433 28.86956177511726 29.358079626306733 21.048307269357576 68-69 20.747466092585086 28.87976160385485 29.41072658952411 20.962045714035956 70-71 20.81509759431513 28.74692515085906 29.376644360160302 21.061332894665508 72-73 20.769941408321895 28.921239560557265 29.342514653069085 20.966304378051756 74-75 20.74194742382517 29.053527812460374 29.207975610032715 20.996549153681745 76-77 20.730078756773956 28.974088741095144 29.218338606316696 21.077493895814204 78-79 20.62945001789262 28.98367864032544 29.229445081997557 21.157426259784383 80-81 20.690806818733336 29.102986794729734 29.100375025397913 21.10583136113901 82-83 20.811606291608364 28.997528896040066 29.248505533249265 20.942359279102305 84-85 20.811421205592723 29.054494258631795 28.99831037032833 21.135774165447156 86-87 20.729530926228005 29.103027924955438 29.061692048128947 21.105749100687614 88-89 20.75493737714911 29.040665882175837 28.952770553707662 21.25162618696739 90-91 20.687795692423116 28.98863422286828 29.15861494810292 21.164955136605684 92-93 20.712662260863844 28.96609670963456 29.102158267462265 21.21908276203934 94-95 20.51526097446811 28.972859050177814 29.090474220087415 21.42140575526667 96-97 20.521309158631876 28.8527916318088 29.15372092812822 21.472178281431102 98-99 20.432097699092914 28.93073340950651 29.083902370005866 21.55326652139471 100-101 20.440282614006815 28.85793291002105 29.05796976270328 21.643814713268856 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 173.0 1 127.5 2 127.0 3 208.5 4 195.5 5 180.0 6 344.0 7 744.0 8 1276.0 9 1638.0 10 1602.0 11 1445.5 12 1523.5 13 1694.0 14 1856.5 15 2142.5 16 2507.5 17 2884.0 18 3226.0 19 3524.0 20 4038.5 21 4754.5 22 5645.0 23 7154.5 24 9115.0 25 11760.5 26 15782.5 27 20695.5 28 26385.0 29 34316.5 30 43352.5 31 51164.5 32 59665.0 33 67579.5 34 73368.0 35 78141.5 36 84493.0 37 91919.0 38 95311.0 39 95678.0 40 95644.0 41 95620.0 42 95454.5 43 96136.5 44 97323.5 45 99020.5 46 99444.0 47 98046.5 48 96011.0 49 91988.0 50 85999.5 51 78911.0 52 72502.5 53 65884.0 54 58109.5 55 49003.5 56 39392.0 57 30770.5 58 23634.5 59 17897.5 60 12851.0 61 9018.5 62 6344.0 63 3936.0 64 2164.5 65 1202.0 66 626.0 67 334.0 68 170.5 69 84.5 70 52.0 71 26.0 72 15.5 73 10.5 74 4.5 75 4.5 76 5.0 77 2.0 78 1.5 79 2.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004400934149686053 2 0.005634840920626068 3 0.0016863392536180205 4 0.17204773409473606 5 0.0022621624133900274 6 6.58083611168008E-4 7 8.2260451396001E-5 8 5.346929340740064E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.673464670370032E-4 24-25 8.431696268090102E-4 26-27 0.0030230715888030363 28-29 3.29041805584004E-4 30-31 0.006334054757492076 32-33 0.019454596755154235 34-35 0.012585849063588152 36-37 0.001007690529601012 38-39 3.7017203128200443E-4 40-41 0.0019331206078060234 42-43 0.011537028308289139 44-45 0.00756796152843209 46-47 0.010426512214443125 48-49 0.010323686650198123 50-51 0.1913995052856453 52-53 0.11438315766613939 54-55 0.017192434341764207 56-57 0.006786487240170082 58-59 0.01737752035740521 60-61 0.1244394978493005 62-63 0.24003599717353089 64-65 0.24542405673996898 66-67 0.25548039692313007 68-69 0.250688725629313 70-71 0.23758874874449987 72-73 0.16079861736633294 74-75 0.26876545982358424 76-77 0.20968189060840653 78-79 0.005429189792136065 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 4.11302256980005E-5 90-91 0.005922752500512071 92-93 0.017151304116066208 94-95 0.0027968553474640337 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2431302.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.493889314576954 #Duplication Level Percentage of deduplicated Percentage of total 1 74.97545317840334 43.856058595277716 2 14.804444604781844 17.319390881517904 3 4.576035250770124 8.0301029837445 4 1.9948990992014974 4.667576284097667 5 1.0619752730628498 3.105953203867798 6 0.6069327915030596 2.1301115716540306 7 0.3867050285964047 1.583391679807589 8 0.28108283103357634 1.315330240536476 9 0.1966481638658564 1.0352444337975775 >10 1.0266903679781896 10.685489887240946 >50 0.06161542955599823 2.431783008340762 >100 0.026250325823271033 2.7445350892473224 >500 8.451036160405162E-4 0.3193809415465758 >1k 2.817012053452514E-4 0.2781864437320002 >5k 1.408506026726257E-4 0.4974647555912386 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6366 0.26183501679347115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5710 0.23485358873558282 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3200 0.1316167222336016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.056511284900025E-5 4.11302256980005E-5 14-15 0.0 0.0 0.0 4.11302256980005E-5 4.11302256980005E-5 16-17 0.0 0.0 0.0 4.11302256980005E-5 4.11302256980005E-5 18-19 0.0 0.0 0.0 4.11302256980005E-5 4.11302256980005E-5 20-21 0.0 0.0 0.0 4.11302256980005E-5 4.11302256980005E-5 22-23 0.0 0.0 0.0 4.11302256980005E-5 4.11302256980005E-5 24-25 0.0 2.056511284900025E-5 0.0 4.11302256980005E-5 4.11302256980005E-5 26-27 0.0 4.11302256980005E-5 0.0 4.11302256980005E-5 4.11302256980005E-5 28-29 0.0 4.11302256980005E-5 0.0 3.084766927350037E-4 4.11302256980005E-5 30-31 0.0 4.11302256980005E-5 0.0 0.001830295043561022 4.11302256980005E-5 32-33 0.0 4.11302256980005E-5 0.0 0.006457445434586077 4.11302256980005E-5 34-35 0.0 4.11302256980005E-5 0.0 0.01326449778760516 4.11302256980005E-5 36-37 0.0 4.11302256980005E-5 0.0 0.024225702936122293 4.11302256980005E-5 38-39 0.0 4.11302256980005E-5 0.0 0.04382425548121953 4.11302256980005E-5 40-41 0.0 4.11302256980005E-5 0.0 0.06484180081289778 4.11302256980005E-5 42-43 0.0 4.11302256980005E-5 0.0 0.08659969020714003 4.11302256980005E-5 44-45 0.0 4.11302256980005E-5 0.0 0.11290246954101135 4.11302256980005E-5 46-47 0.0 4.11302256980005E-5 0.0 0.14580665009941174 4.11302256980005E-5 48-49 0.0 4.11302256980005E-5 0.0 0.1823508556320852 4.11302256980005E-5 50-51 0.0 4.11302256980005E-5 0.0 0.22241169546193768 4.11302256980005E-5 52-53 0.0 4.11302256980005E-5 0.0 0.2605394146839841 4.11302256980005E-5 54-55 0.0 4.11302256980005E-5 0.0 0.3043431050523547 4.11302256980005E-5 56-57 0.0 4.11302256980005E-5 0.0 0.3564139707860233 6.169533854700075E-5 58-59 0.0 4.11302256980005E-5 0.0 0.41463380526154303 8.2260451396001E-5 60-61 0.0 4.11302256980005E-5 0.0 0.47842678531914173 8.2260451396001E-5 62-63 4.11302256980005E-5 4.11302256980005E-5 0.0 0.5468880459934636 8.2260451396001E-5 64-65 4.11302256980005E-5 4.11302256980005E-5 0.0 0.6192566781090955 8.2260451396001E-5 66-67 4.11302256980005E-5 4.11302256980005E-5 0.0 0.7033062943229594 8.2260451396001E-5 68-69 4.11302256980005E-5 4.11302256980005E-5 0.0 0.7966307764317226 8.2260451396001E-5 70-71 4.11302256980005E-5 4.11302256980005E-5 0.0 0.9014100263973788 8.2260451396001E-5 72-73 4.11302256980005E-5 4.11302256980005E-5 0.0 1.0126467217976212 8.2260451396001E-5 74-75 4.11302256980005E-5 4.11302256980005E-5 0.0 1.1265568818682334 8.2260451396001E-5 76-77 4.11302256980005E-5 4.11302256980005E-5 0.0 1.252559328294058 8.2260451396001E-5 78-79 4.11302256980005E-5 4.11302256980005E-5 0.0 1.3988595411018458 8.2260451396001E-5 80-81 4.11302256980005E-5 4.11302256980005E-5 0.0 1.5629485765240188 8.2260451396001E-5 82-83 4.11302256980005E-5 4.11302256980005E-5 0.0 1.7395617656712328 8.2260451396001E-5 84-85 4.11302256980005E-5 4.11302256980005E-5 0.0 1.9174499918150851 8.2260451396001E-5 86-87 4.11302256980005E-5 4.11302256980005E-5 0.0 2.1145871635856013 8.2260451396001E-5 88-89 4.11302256980005E-5 4.11302256980005E-5 0.0 2.339178761009533 8.2260451396001E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3365 0.0 58.611797 1 GTATCAA 6280 0.0 41.47085 1 ATCAACG 8015 0.0 32.431053 3 TCAACGC 8080 0.0 32.170166 4 CAACGCA 8155 0.0 31.826443 5 TATCAAC 8200 0.0 31.467571 2 AACGCAG 8585 0.0 30.39565 6 ACGCAGA 9885 0.0 26.110264 7 CGCAGAG 9945 0.0 25.952736 8 GTACATG 6355 0.0 24.60381 1 GTGGTAT 1625 0.0 23.104454 1 TACATGG 6605 0.0 22.806767 2 ACATGGG 6385 0.0 22.401798 3 GCAGAGT 11275 0.0 22.386393 9 TGGTATC 1860 0.0 20.438751 2 AGAGTAC 10410 0.0 20.167477 10-11 GAGTACT 8010 0.0 20.138885 12-13 ACCGTGC 365 5.456968E-12 19.49788 8 CAGAGTA 10990 0.0 19.448502 10-11 GTACTAG 765 0.0 19.258492 1 >>END_MODULE