FastQCFastQC Report
Sat 18 Jun 2016
SRR1931038_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931038_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2994548
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1336264.4623095038049145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.058.127851
GTATCAA44100.045.103061
ATCAACG52450.037.4787143
TCAACGC54700.036.025624
CAACGCA55150.035.5390745
TATCAAC55750.034.5683172
AACGCAG60050.033.191466
ACGCAGA67900.028.4489867
CGCAGAG70850.027.1085268
GTACATG46650.025.604551
TACATGG49950.023.6008472
GCAGAGT77200.023.1680019
GTGGTAT11650.022.8818071
ACATGGG49000.022.6303983
CAGAGTA72050.020.97682610-11
GAGTACT51600.020.57037512-13
AGAGTAC69400.019.6000310-11
GTACTAG6300.019.529161
GAGTACA36800.019.5026261
GCGGTAT2151.2425535E-519.0749951
Produced by FastQC (version 0.11.5)