##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931038_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2994548 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.107746811872776 28.0 2.0 31.0 2.0 33.0 2 19.7982887567673 27.0 2.0 31.0 2.0 33.0 3 20.460752674527175 27.0 2.0 31.0 2.0 33.0 4 21.970624949074118 30.0 2.0 35.0 2.0 37.0 5 21.314002647478016 30.0 2.0 35.0 2.0 37.0 6 22.30189564501888 30.0 2.0 35.0 2.0 37.0 7 22.487161334531955 30.0 2.0 35.0 2.0 37.0 8 22.921215488948583 32.0 2.0 35.0 2.0 37.0 9 24.223152208613786 32.0 2.0 37.0 2.0 39.0 10-11 24.60411537901546 32.0 2.0 37.0 2.0 39.0 12-13 24.508986498129268 32.0 2.0 37.0 2.0 39.0 14-15 24.466083863073827 32.0 2.0 38.0 2.0 40.0 16-17 23.281215228475215 31.5 2.0 38.0 2.0 40.0 18-19 22.214619201295154 29.0 2.0 38.0 2.0 40.0 20-21 21.87653512316383 28.0 2.0 37.0 2.0 40.0 22-23 21.51072615967418 27.0 2.0 37.0 2.0 40.0 24-25 21.345175799486267 27.0 2.0 37.0 2.0 39.5 26-27 21.070311779941413 27.0 2.0 37.0 2.0 39.0 28-29 20.866926995326175 26.0 2.0 37.0 2.0 39.0 30-31 20.853964104098516 26.0 2.0 36.5 2.0 39.0 32-33 20.71923625869413 25.5 2.0 36.5 2.0 39.0 34-35 20.394188204697336 25.0 2.0 36.0 2.0 39.0 36-37 19.939645315419888 24.0 2.0 36.0 2.0 39.0 38-39 19.82232543943193 24.0 2.0 35.5 2.0 39.0 40-41 19.681279946088694 24.0 2.0 35.0 2.0 39.0 42-43 19.53971083448988 24.0 2.0 35.0 2.0 39.0 44-45 19.14948750195355 23.0 2.0 35.0 2.0 38.5 46-47 18.801039255340037 20.0 2.0 35.0 2.0 38.0 48-49 18.555228535324865 18.5 2.0 35.0 2.0 38.0 50-51 16.28303687234267 11.0 2.0 32.5 2.0 37.5 52-53 18.152951296823424 19.0 2.0 34.0 2.0 38.0 54-55 18.936631838928612 22.0 2.0 35.5 2.0 39.0 56-57 18.91132651739094 21.5 2.0 36.0 2.0 39.0 58-59 18.63138376810123 20.0 2.0 35.0 2.0 39.0 60-61 18.335245419342083 18.5 2.0 35.0 2.0 39.0 62-63 17.89227572909167 15.0 2.0 34.0 2.0 38.5 64-65 17.53848928118701 13.0 2.0 34.0 2.0 38.0 66-67 17.18014621906211 8.0 2.0 34.0 2.0 38.0 68-69 16.738870774487502 7.5 2.0 33.5 2.0 37.5 70-71 16.384980972086606 6.5 2.0 33.0 2.0 37.0 72-73 15.985307799374063 2.0 2.0 32.0 2.0 36.0 74-75 15.531597089109944 2.0 2.0 32.0 2.0 36.0 76-77 15.120723561619315 2.0 2.0 31.0 2.0 35.5 78-79 14.74883371380255 2.0 2.0 31.0 2.0 35.0 80-81 14.31676633668921 2.0 2.0 31.0 2.0 35.0 82-83 13.863629001772555 2.0 2.0 30.0 2.0 34.0 84-85 13.468041921518706 2.0 2.0 30.0 2.0 34.0 86-87 12.985476105241926 2.0 2.0 29.0 2.0 34.0 88-89 12.568522695244825 2.0 2.0 29.0 2.0 34.0 90-91 12.091084363984148 2.0 2.0 29.0 2.0 34.0 92-93 11.547577798051659 2.0 2.0 27.0 2.0 34.0 94-95 10.985438536967848 2.0 2.0 26.0 2.0 33.5 96-97 10.347781701946337 2.0 2.0 25.0 2.0 33.0 98-99 9.67708599094087 2.0 2.0 22.0 2.0 32.5 100-101 8.753379474965838 2.0 2.0 10.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.625706633593346 1101 2 9.35014774033463 1101 3 8.568144044754792 1101 4 10.607954869674014 1101 5 11.265555863788503 1101 6 10.267620380295096 1101 7 9.96315243359437 1101 8 9.51099483704552 1101 9 9.14383290774462 1101 10-11 8.744665207960509 1101 12-13 8.81587489081063 1101 14-15 9.378780682965559 1101 16-17 10.263463452111143 1101 18-19 11.301671240675024 1101 20-21 11.279057103357875 1101 22-23 11.471318000315069 1101 24-25 11.326263684574371 1101 26-27 11.14175324982859 1101 28-29 11.348211938231511 1101 30-31 10.856750301340792 1101 32-33 10.899070874339525 1101 34-35 10.856884148760688 1101 36-37 11.21498218334198 1101 38-39 10.895591369430477 1101 40-41 10.629801553571749 1101 42-43 10.388965278724335 1101 44-45 10.334962831389046 1101 46-47 9.971133608019453 1101 48-49 10.231436552896366 1101 50-51 11.847671389022935 1101 52-53 11.059067876224542 1101 54-55 10.73645178007187 1101 56-57 10.641761072131128 1101 58-59 10.452807591890632 1101 60-61 10.112613752897559 1101 62-63 10.056021589064766 1101 64-65 9.901783926810982 1101 66-67 9.681060608223149 1101 68-69 9.69644125913474 1101 70-71 9.33374037631981 1101 72-73 9.192775270802057 1101 74-75 8.810162743483064 1101 76-77 8.717294948983417 1101 78-79 8.524488923110098 1101 80-81 8.351244631608122 1101 82-83 8.005824811247958 1101 84-85 7.804051008063384 1101 86-87 7.537538435191921 1101 88-89 7.400056066878387 1101 90-91 7.078118710342618 1101 92-93 6.723447648824743 1101 94-95 6.228139863963953 1101 96-97 5.998565135082519 1101 98-99 5.634986325061673 1101 100-101 5.130768174858503 1102 1 7.773563633929037 1102 2 9.154348832354799 1102 3 8.50528576845451 1102 4 10.500711437038095 1102 5 11.26383063548504 1102 6 10.223308656150078 1102 7 9.759254575844729 1102 8 9.257604539172014 1102 9 9.12845410896681 1102 10-11 8.27621782137578 1102 12-13 8.535776309700385 1102 14-15 9.050938707327067 1102 16-17 9.747458767008933 1102 18-19 10.890543512765486 1102 20-21 10.957349498780157 1102 22-23 11.174070808293532 1102 24-25 11.038566370351397 1102 26-27 11.248850139215303 1102 28-29 11.042711171368225 1102 30-31 10.978331208628692 1102 32-33 10.72649462903233 1102 34-35 10.379528773781242 1102 36-37 10.633339556619003 1102 38-39 10.676690919074918 1102 40-41 10.329756284660071 1102 42-43 9.860388756387252 1102 44-45 10.0261233083429 1102 46-47 9.802329004870135 1102 48-49 9.191538520879071 1102 50-51 11.16790976104626 1102 52-53 10.49886744916176 1102 54-55 10.175958373554689 1102 56-57 10.020872471975615 1102 58-59 10.139686147892636 1102 60-61 9.962430632710259 1102 62-63 9.99992892235014 1102 64-65 9.848514599372777 1102 66-67 9.627840413985371 1102 68-69 9.296917637090864 1102 70-71 9.00576786051155 1102 72-73 8.665502223370426 1102 74-75 8.306534861430146 1102 76-77 8.12240658946284 1102 78-79 7.9899875148391075 1102 80-81 7.4467596267611675 1102 82-83 7.400572022537418 1102 84-85 7.360378529335167 1102 86-87 6.970698710474865 1102 88-89 6.449358771495151 1102 90-91 6.275985601544797 1102 92-93 6.128652524636566 1102 94-95 5.8862955149456795 1102 96-97 5.6825727707458125 1102 98-99 5.021361410268703 1102 100-101 4.568270904480748 1103 1 7.707793976890304 1103 2 9.060116075609802 1103 3 8.662988288308728 1103 4 10.47266480001677 1103 5 11.247711829730033 1103 6 10.272748726319794 1103 7 10.158810575627147 1103 8 9.424985141870014 1103 9 9.42388423842721 1103 10-11 8.668688006047233 1103 12-13 8.45292275837858 1103 14-15 9.135281750190419 1103 16-17 10.056930488342697 1103 18-19 10.846361496474142 1103 20-21 11.212597778534299 1103 22-23 11.504625740571601 1103 24-25 11.377619266211923 1103 26-27 11.133299592544287 1103 28-29 10.940933215673056 1103 30-31 10.585566950306237 1103 32-33 10.864869880609792 1103 34-35 10.81691403406192 1103 36-37 10.915978757146632 1103 38-39 10.572403988183925 1103 40-41 10.286933296599802 1103 42-43 10.00374668651671 1103 44-45 10.154512804644028 1103 46-47 10.011950397793328 1103 48-49 10.150764699213493 1103 50-51 11.670265324987732 1103 52-53 10.805651286329951 1103 54-55 10.415658571416959 1103 56-57 10.140018621480618 1103 58-59 10.136723738324534 1103 60-61 9.799262677559035 1103 62-63 9.862949245729165 1103 64-65 9.487800544352108 1103 66-67 9.487082400903343 1103 68-69 9.338425792960102 1103 70-71 9.056233363802455 1103 72-73 9.037470120046885 1103 74-75 8.775132300099644 1103 76-77 8.406680193547912 1103 78-79 8.15500320443332 1103 80-81 7.699337020826661 1103 82-83 7.298429449511961 1103 84-85 6.966150702148484 1103 86-87 6.867118916224429 1103 88-89 6.619798675079794 1103 90-91 6.532716148487793 1103 92-93 6.198071329711633 1103 94-95 5.954447933488979 1103 96-97 5.709524280890506 1103 98-99 5.3630796673128724 1103 100-101 4.54891431383127 1104 1 7.912331540659398 1104 2 9.496442233597165 1104 3 8.76520171292966 1104 4 10.872311255749924 1104 5 11.561069815772992 1104 6 10.706744724927713 1104 7 10.258918156335358 1104 8 9.698685885244874 1104 9 9.461895195248129 1104 10-11 8.907073289303234 1104 12-13 8.89013308629151 1104 14-15 9.543628780237146 1104 16-17 10.534342559978501 1104 18-19 11.44471784963245 1104 20-21 11.471421334627184 1104 22-23 11.773205782432822 1104 24-25 11.510073838206466 1104 26-27 11.62647199052611 1104 28-29 11.44047963660433 1104 30-31 11.294873794896855 1104 32-33 11.122724633046335 1104 34-35 11.228275097020344 1104 36-37 11.224913625666431 1104 38-39 10.743585450324918 1104 40-41 10.587641065502087 1104 42-43 10.291122426469109 1104 44-45 10.31595159456105 1104 46-47 10.114145369993054 1104 48-49 10.046265029913869 1104 50-51 11.572622328001145 1104 52-53 10.950357750573904 1104 54-55 10.542924095779103 1104 56-57 10.45706966891839 1104 58-59 10.360912512160112 1104 60-61 10.064253981211259 1104 62-63 9.926387329712217 1104 64-65 9.838068424026652 1104 66-67 9.612030239310442 1104 68-69 9.434906699755373 1104 70-71 9.23990113610246 1104 72-73 9.115529634568835 1104 74-75 8.671128990621819 1104 76-77 8.431892282997003 1104 78-79 8.206263618196388 1104 80-81 7.881482829178783 1104 82-83 7.613683808406812 1104 84-85 7.14125036584022 1104 86-87 6.980949737829308 1104 88-89 6.687497618982784 1104 90-91 6.264121056048378 1104 92-93 5.8649630678101285 1104 94-95 5.672185163588814 1104 96-97 5.379451081475704 1104 98-99 4.766729164499859 1104 100-101 4.196232362649573 1105 1 7.650881513911774 1105 2 8.833849970145131 1105 3 8.256036734595437 1105 4 10.432545167617189 1105 5 10.38373939156633 1105 6 10.220435980773427 1105 7 9.89762116011968 1105 8 9.607676141494675 1105 9 9.494486653693738 1105 10-11 8.858756050150106 1105 12-13 8.496401469089111 1105 14-15 9.138678526065295 1105 16-17 10.092082339866746 1105 18-19 10.515057070616233 1105 20-21 10.853724140768545 1105 22-23 11.220150703734529 1105 24-25 11.128615793118975 1105 26-27 11.302575176487746 1105 28-29 11.118418673428017 1105 30-31 10.796284142362214 1105 32-33 10.642536614476462 1105 34-35 10.772814952571263 1105 36-37 10.745833978013383 1105 38-39 10.578958959427244 1105 40-41 10.35721620452432 1105 42-43 10.13905512634566 1105 44-45 10.12370109004409 1105 46-47 9.770631183836702 1105 48-49 9.627395897389587 1105 50-51 11.353417095380063 1105 52-53 10.511564907279311 1105 54-55 10.13223896393055 1105 56-57 10.168656784691404 1105 58-59 10.028290895715138 1105 60-61 9.938651366486937 1105 62-63 10.118943875465334 1105 64-65 9.695029687355358 1105 66-67 9.454237578827652 1105 68-69 9.196453386757376 1105 70-71 9.101640489297644 1105 72-73 8.748133326732797 1105 74-75 8.4944973952696 1105 76-77 8.227176174659794 1105 78-79 7.855009473702101 1105 80-81 7.684311673312486 1105 82-83 7.378718294802798 1105 84-85 7.179281665124558 1105 86-87 6.932916531115952 1105 88-89 6.565061197636867 1105 90-91 6.3379381625539 1105 92-93 6.237774035249403 1105 94-95 5.8409656660952365 1105 96-97 5.649025427388153 1105 98-99 5.119924643456871 1105 100-101 4.594917941812042 1106 1 7.5753132210131575 1106 2 8.970857900053261 1106 3 8.413338162708563 1106 4 10.07982862782826 1106 5 10.86776596233016 1106 6 10.090193872116597 1106 7 9.230491119064354 1106 8 8.919153352079995 1106 9 8.487795384761714 1106 10-11 8.369644356896416 1106 12-13 7.992944204421644 1106 14-15 8.656073505442453 1106 16-17 9.702851719801618 1106 18-19 10.887232063151085 1106 20-21 11.054333526667616 1106 22-23 11.229933514586957 1106 24-25 10.854198917170464 1106 26-27 10.988600859761736 1106 28-29 10.997017855176658 1106 30-31 10.685113845433808 1106 32-33 10.782389825718475 1106 34-35 10.615198659875684 1106 36-37 10.898476481004398 1106 38-39 10.642511540028014 1106 40-41 10.568689707321418 1106 42-43 10.2107370821861 1106 44-45 9.657076041813102 1106 46-47 9.472957751547064 1106 48-49 9.56600602801873 1106 50-51 11.351681077211502 1106 52-53 10.533427870599173 1106 54-55 10.11320204903242 1106 56-57 9.915376680920918 1106 58-59 9.719409037744445 1106 60-61 9.662485187781062 1106 62-63 9.508351953843615 1106 64-65 9.43598377462678 1106 66-67 8.900090883656617 1106 68-69 8.56819791895942 1106 70-71 8.427677510075931 1106 72-73 8.065466463374902 1106 74-75 7.926340614121505 1106 76-77 7.977691398600287 1106 78-79 7.5829218620372 1106 80-81 7.138273732526855 1106 82-83 6.787562519714456 1106 84-85 6.542861231285576 1106 86-87 6.136350874910798 1106 88-89 5.920283468635342 1106 90-91 5.728271403489945 1106 92-93 5.567900528328133 1106 94-95 5.423132731497844 1106 96-97 4.8101206961314205 1106 98-99 4.577057654708321 1106 100-101 4.1914945231044065 1107 1 7.242562049734392 1107 2 8.147033366340914 1107 3 8.330508199258311 1107 4 9.965024457547624 1107 5 10.810545860105542 1107 6 9.844724790164687 1107 7 9.459528915939018 1107 8 8.17453591286386 1107 9 5.543254469680722 1107 10-11 7.564193666657353 1107 12-13 7.599273969835995 1107 14-15 8.203432215124185 1107 16-17 9.015659095180883 1107 18-19 10.381982982687642 1107 20-21 10.796496773835852 1107 22-23 10.087773586017345 1107 24-25 10.299837718932501 1107 26-27 10.185390272685929 1107 28-29 10.533595930314839 1107 30-31 10.29919710416413 1107 32-33 10.713347412260756 1107 34-35 10.893166346150686 1107 36-37 10.96863082136812 1107 38-39 10.524204039072199 1107 40-41 10.142019151283346 1107 42-43 9.845912489760085 1107 44-45 9.273155270704311 1107 46-47 9.59800073132659 1107 48-49 9.905654680716783 1107 50-51 11.351581273794288 1107 52-53 10.273310906688835 1107 54-55 10.154431955065025 1107 56-57 9.756874519609926 1107 58-59 9.687057119407893 1107 60-61 9.473942099705255 1107 62-63 9.598811061344808 1107 64-65 9.722156205083973 1107 66-67 9.62459476788976 1107 68-69 9.38316866718166 1107 70-71 9.213596823750464 1107 72-73 8.980803857835118 1107 74-75 9.119346873705434 1107 76-77 8.617352314403908 1107 78-79 8.380581601996525 1107 80-81 8.026741572851599 1107 82-83 7.867870839127022 1107 84-85 7.7662900564761355 1107 86-87 7.470657172923703 1107 88-89 7.445335599106928 1107 90-91 6.76513514320499 1107 92-93 6.210215832114416 1107 94-95 6.007380512209561 1107 96-97 5.720087655456593 1107 98-99 5.467425785479174 1107 100-101 4.994861909056052 1108 1 6.695853210430702 1108 2 7.74814925656883 1108 3 7.752472381964292 1108 4 9.1233510551804 1108 5 10.859335113480562 1108 6 9.383170900450516 1108 7 8.685037452432276 1108 8 5.820883674608201 1108 9 7.029073676164394 1108 10-11 6.02806338827407 1108 12-13 4.670935060578742 1108 14-15 6.984163221953338 1108 16-17 8.29696782222182 1108 18-19 7.721570243291975 1108 20-21 8.087014755195423 1108 22-23 9.039520502820391 1108 24-25 8.790028367573182 1108 26-27 7.039219195664398 1108 28-29 8.956639782880561 1108 30-31 9.217199860057487 1108 32-33 9.798339171180473 1108 34-35 9.07353654724314 1108 36-37 8.871595086203374 1108 38-39 8.711418054947028 1108 40-41 9.00087014334865 1108 42-43 8.211835870332962 1108 44-45 7.454172021386828 1108 46-47 8.507029456573495 1108 48-49 9.12002183778387 1108 50-51 10.61656418682832 1108 52-53 9.85848104922292 1108 54-55 9.369689525471461 1108 56-57 9.244549304001172 1108 58-59 9.418027301045612 1108 60-61 9.184364576176577 1108 62-63 9.16891489953888 1108 64-65 9.165020782845346 1108 66-67 8.539291529336456 1108 68-69 8.748528792390939 1108 70-71 8.8378095816689 1108 72-73 8.561455822520697 1108 74-75 8.468397300107798 1108 76-77 8.029891677721478 1108 78-79 7.761456538919106 1108 80-81 7.5762245079226815 1108 82-83 7.5944520072149615 1108 84-85 7.36860885901193 1108 86-87 7.00048102019665 1108 88-89 6.672866641739011 1108 90-91 6.617221100516794 1108 92-93 6.314243622485568 1108 94-95 6.115664996747412 1108 96-97 5.609793042418465 1108 98-99 5.561952597580019 1108 100-101 4.9431974030558585 1109 1 6.587830468738748 1109 2 8.149037972774746 1109 3 7.8901812942091 1109 4 8.99528995163783 1109 5 10.044380483746192 1109 6 9.198706663039843 1109 7 7.488952328469399 1109 8 8.07264472946073 1109 9 7.248596415397255 1109 10-11 5.633759139453204 1109 12-13 6.01230851319448 1109 14-15 7.494147778849371 1109 16-17 6.408509968616542 1109 18-19 5.323495909829131 1109 20-21 7.879699297828719 1109 22-23 9.058067178864398 1109 24-25 8.539791507736567 1109 26-27 9.044000852797982 1109 28-29 9.510617021515863 1109 30-31 7.22466025636491 1109 32-33 5.989600046519207 1109 34-35 7.871820542974156 1109 36-37 6.323155675795508 1109 38-39 6.565868914891848 1109 40-41 7.491630003081983 1109 42-43 6.47010860341809 1109 44-45 7.034422602962319 1109 46-47 6.43544575150727 1109 48-49 7.39580591958314 1109 50-51 8.842520110045601 1109 52-53 9.423138128705727 1109 54-55 9.41597944269839 1109 56-57 8.550416896598318 1109 58-59 8.238737831373605 1109 60-61 8.301584227280465 1109 62-63 8.721747430388202 1109 64-65 8.739399247092596 1109 66-67 8.259872022827235 1109 68-69 7.912640395867115 1109 70-71 7.406082462299608 1109 72-73 6.994219056129614 1109 74-75 6.389030811643403 1109 76-77 6.094002051651158 1109 78-79 6.819313045925064 1109 80-81 6.728771242984919 1109 82-83 6.287693239318912 1109 84-85 6.35534500325249 1109 86-87 6.57244888476948 1109 88-89 6.541919005665102 1109 90-91 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-14.89934057559796 2313 68-69 -14.4757060608258 2313 70-71 -14.135019899905117 2313 72-73 -13.735702203712906 2313 74-75 -13.291284148986518 2313 76-77 -12.879383775120496 2313 78-79 -12.523521599744228 2313 80-81 -12.102882300322165 2313 82-83 -11.666322508712582 2313 84-85 -11.275363658164833 2313 86-87 -10.79574009160847 2313 88-89 -10.391624301421514 2313 90-91 -9.926813877621868 2313 92-93 -9.396249819113434 2313 94-95 -8.841080787137656 2313 96-97 -8.202355614670854 2313 98-99 -7.544231037647963 2313 100-101 -6.643154876769252 2314 1 -18.78665772023763 2314 2 -17.50450533431187 2314 3 -18.14477933571216 2314 4 -19.63242658379524 2314 5 -18.937666760348296 2314 6 -19.962238218849922 2314 7 -20.14569334082194 2314 8 -20.599166294161318 2314 9 -21.896285474366525 2314 10-11 -22.28986290779089 2314 12-13 -22.231313534049615 2314 14-15 -22.189946709339605 2314 16-17 -20.967710503824403 2314 18-19 -19.875244247651185 2314 20-21 -19.55274164705317 2314 22-23 -19.178468254206468 2314 24-25 -19.00619274423194 2314 26-27 -18.745290485784697 2314 28-29 -18.538831797381775 2314 30-31 -18.530457853871308 2314 32-33 -18.396226725134333 2314 34-35 -18.076525913718758 2314 36-37 -17.617474245464333 2314 38-39 -17.506414549675082 2314 40-41 -17.394039288256597 2314 42-43 -17.233198483196084 2314 44-45 -16.834553774990436 2314 46-47 -16.4826644847132 2314 48-49 -16.25458778120426 2314 50-51 -14.046771228537075 2314 52-53 -15.874342186254903 2314 54-55 -16.62921740769531 2314 56-57 -16.612102788441053 2314 58-59 -16.35071098752403 2314 60-61 -16.044242544373077 2314 62-63 -15.599354931816524 2314 64-65 -15.248002327710555 2314 66-67 -14.89934057559796 2314 68-69 -14.4757060608258 2314 70-71 -14.135019899905117 2314 72-73 -13.735702203712906 2314 74-75 -13.291284148986518 2314 76-77 -12.879383775120496 2314 78-79 -12.523521599744228 2314 80-81 -12.102882300322165 2314 82-83 -11.666322508712582 2314 84-85 -11.275363658164833 2314 86-87 -10.79574009160847 2314 88-89 -10.391624301421514 2314 90-91 -9.926813877621868 2314 92-93 -9.396249819113434 2314 94-95 -8.841080787137656 2314 96-97 -8.202355614670854 2314 98-99 -7.544231037647963 2314 100-101 -6.643154876769252 2315 1 -18.78665772023763 2315 2 -17.50450533431187 2315 3 -18.14477933571216 2315 4 -19.63242658379524 2315 5 -18.937666760348296 2315 6 -19.962238218849922 2315 7 -20.14569334082194 2315 8 -20.599166294161318 2315 9 -21.896285474366525 2315 10-11 -22.28986290779089 2315 12-13 -22.231313534049615 2315 14-15 -22.189946709339605 2315 16-17 -20.967710503824403 2315 18-19 -19.875244247651185 2315 20-21 -19.55274164705317 2315 22-23 -19.178468254206468 2315 24-25 -19.00619274423194 2315 26-27 -18.745290485784697 2315 28-29 -18.538831797381775 2315 30-31 -18.530457853871308 2315 32-33 -18.396226725134333 2315 34-35 -18.076525913718758 2315 36-37 -17.617474245464333 2315 38-39 -17.506414549675082 2315 40-41 -17.394039288256597 2315 42-43 -17.233198483196084 2315 44-45 -16.834553774990436 2315 46-47 -16.4826644847132 2315 48-49 -16.25458778120426 2315 50-51 -14.046771228537075 2315 52-53 -15.874342186254903 2315 54-55 -16.62921740769531 2315 56-57 -16.612102788441053 2315 58-59 -16.35071098752403 2315 60-61 -16.044242544373077 2315 62-63 -15.599354931816524 2315 64-65 -15.248002327710555 2315 66-67 -14.89934057559796 2315 68-69 -14.4757060608258 2315 70-71 -14.135019899905117 2315 72-73 -13.735702203712906 2315 74-75 -13.291284148986518 2315 76-77 -12.879383775120496 2315 78-79 -12.523521599744228 2315 80-81 -12.102882300322165 2315 82-83 -11.666322508712582 2315 84-85 -11.275363658164833 2315 86-87 -10.79574009160847 2315 88-89 -10.391624301421514 2315 90-91 -9.926813877621868 2315 92-93 -9.396249819113434 2315 94-95 -8.841080787137656 2315 96-97 -8.202355614670854 2315 98-99 -7.544231037647963 2315 100-101 -6.643154876769252 2316 1 -18.78665772023763 2316 2 -17.50450533431187 2316 3 -18.14477933571216 2316 4 -19.63242658379524 2316 5 -18.937666760348296 2316 6 -19.962238218849922 2316 7 -20.14569334082194 2316 8 -20.599166294161318 2316 9 -21.896285474366525 2316 10-11 -22.28986290779089 2316 12-13 -22.231313534049615 2316 14-15 -22.189946709339605 2316 16-17 -20.967710503824403 2316 18-19 -19.875244247651185 2316 20-21 -19.55274164705317 2316 22-23 -19.178468254206468 2316 24-25 -19.00619274423194 2316 26-27 -18.745290485784697 2316 28-29 -18.538831797381775 2316 30-31 -18.530457853871308 2316 32-33 -18.396226725134333 2316 34-35 -18.076525913718758 2316 36-37 -17.617474245464333 2316 38-39 -17.506414549675082 2316 40-41 -17.394039288256597 2316 42-43 -17.233198483196084 2316 44-45 -16.834553774990436 2316 46-47 -16.4826644847132 2316 48-49 -16.25458778120426 2316 50-51 -14.046771228537075 2316 52-53 -15.874342186254903 2316 54-55 -16.62921740769531 2316 56-57 -16.612102788441053 2316 58-59 -16.35071098752403 2316 60-61 -16.044242544373077 2316 62-63 -15.599354931816524 2316 64-65 -15.248002327710555 2316 66-67 -14.89934057559796 2316 68-69 -14.4757060608258 2316 70-71 -14.135019899905117 2316 72-73 -13.735702203712906 2316 74-75 -13.291284148986518 2316 76-77 -12.879383775120496 2316 78-79 -12.523521599744228 2316 80-81 -12.102882300322165 2316 82-83 -11.666322508712582 2316 84-85 -11.275363658164833 2316 86-87 -10.79574009160847 2316 88-89 -10.391624301421514 2316 90-91 -9.926813877621868 2316 92-93 -9.396249819113434 2316 94-95 -8.841080787137656 2316 96-97 -8.202355614670854 2316 98-99 -7.544231037647963 2316 100-101 -6.643154876769252 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 764011.0 3 31259.0 4 117254.0 5 62257.0 6 35770.0 7 35558.0 8 34905.0 9 34366.0 10 36636.0 11 38490.0 12 39643.0 13 40753.0 14 41560.0 15 42859.0 16 44501.0 17 45317.0 18 44568.0 19 45738.0 20 48257.0 21 50941.0 22 54447.0 23 57985.0 24 61967.0 25 66310.0 26 71152.0 27 76351.0 28 82157.0 29 88386.0 30 96169.0 31 103463.0 32 111854.0 33 118967.0 34 122870.0 35 116406.0 36 87560.0 37 38487.0 38 5315.0 39 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.328237371048864 20.434245932059277 14.108352677882854 23.129164019009 2 14.940910426232728 20.669637754605645 41.719902857137455 22.66954896202417 3 18.556438374952585 26.00567191931187 30.401886820296337 25.036002885439206 4 12.19335246205372 17.353895051378267 39.62148606778912 30.831266418778895 5 12.608301345802941 37.993166532842125 35.98550140639526 13.41303071495967 6 29.53159957440449 38.774338464421064 18.169805470731387 13.524256490443062 7 26.153927348613625 33.20725590273273 23.079998328741034 17.558818419912612 8 24.473818685187034 36.857323000724094 20.73041357874325 17.93844473534562 9 25.368709535578365 16.921595681773386 21.575819143935835 36.13387563871242 10-11 23.860234682017158 27.34038975538321 29.121946194864247 19.677429367735385 12-13 24.50165782552428 24.888480482624846 29.4407765506898 21.16908514116108 14-15 21.71432592474464 26.621807220412467 26.957035154380353 24.706831700462537 16-17 21.171249217279687 29.731864554863375 27.732942342281312 21.363943885575626 18-19 20.964475142676548 28.53702719786008 29.931043485430042 20.567454174033326 20-21 21.524033388943867 27.725554363321464 30.112950105356784 20.63746214237789 22-23 21.220056434638078 27.62633663296753 29.851674590708317 21.30193234168608 24-25 21.187715905871354 27.82601069088501 29.97394550229297 21.012327900950662 26-27 21.07796543648388 28.061880772519103 29.768606300995636 21.091547490001382 28-29 21.004173050207307 28.27846744719908 29.748242589366203 20.969116913227413 30-31 21.345827879660312 27.989812796073675 29.660739106804137 21.003620217461876 32-33 21.211079641487256 28.12625100847907 29.580324945651814 21.082344404381864 34-35 21.28453635318783 28.152473514389097 29.651396687131587 20.911593445291484 36-37 21.36632704808446 27.925660412152503 29.60197777880227 21.106034760960767 38-39 21.288470771462677 28.069423073236983 29.700940117588022 20.941166037712318 40-41 21.35344781706257 28.049215881069383 29.631357661125797 20.965978640742254 42-43 21.271863897546254 28.08614000970981 29.353059263041608 21.288936829702326 44-45 21.278040079184443 28.4196810744242 29.205901605214034 21.096377241177322 46-47 21.468196298204354 28.3025572931536 28.958196026088107 21.271050382553934 48-49 21.406068807032273 28.401500265582634 28.815120027964735 21.37731089942036 50-51 21.607460131004306 28.565702450172225 28.495867142768326 21.330970276055137 52-53 21.156975604861447 28.747829711289963 28.64440197370311 21.450792710145482 54-55 21.083610395488094 28.686007345773568 28.769343580281298 21.46103867845704 56-57 21.1606886676368 28.763440258579365 28.743096067998387 21.332775005785447 58-59 21.157734357579912 28.651915009816754 28.782332234110886 21.408018398492445 60-61 21.087400019429424 28.820949059005745 28.819274868394302 21.27237605317053 62-63 21.068201381665546 28.686463739852268 28.75221023403202 21.49312464445017 64-65 20.860902874995364 28.659104612016655 28.787992488836327 21.69200002415165 66-67 20.931493980835654 28.664692510702267 28.576113783618297 21.827699724843782 68-69 20.970356745893447 28.692027005781775 28.682154349850425 21.655461898474357 70-71 21.03244249506577 28.454235892306052 28.67607413148389 21.83724748114429 72-73 21.053946946393168 28.635407674970782 28.571410120353292 21.739235258282765 74-75 20.93695427884896 28.81524139579002 28.391152956130593 21.85665136923042 76-77 21.02271304595705 28.685324445945366 28.45417274721258 21.837789760885006 78-79 20.81298893713301 28.764173386081747 28.491462228489304 21.931375448295938 80-81 20.971398769899956 28.818736550536755 28.21875571391182 21.991108965651474 82-83 21.066918983957684 28.636784462895314 28.492732152423685 21.803564400723317 84-85 21.077338896066582 28.746045435475438 28.178602438338817 21.998013230119167 86-87 20.984873791622483 28.813962967932323 28.258837052990955 21.942326187454245 88-89 21.061917607312157 28.671021135060105 28.188649398947174 22.078411858680564 90-91 21.00075877855781 28.667992935070234 28.30267400365149 22.028574282720463 92-93 20.995096426740922 28.618712117155365 28.180905218833324 22.205286237270386 94-95 20.834733241835313 28.53068823421701 28.29575558941468 22.338822934533 96-97 20.849009438592812 28.45250901216409 28.23492474019742 22.463556809045674 98-99 20.683367608472388 28.472012656447987 27.989892602737022 22.854727132342603 100-101 20.91390887531827 28.56018770448301 27.94953090674567 22.576372513453048 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 180206.0 1 163744.0 2 108960.0 3 55135.5 4 34040.0 5 26674.0 6 24699.0 7 24957.0 8 25969.5 9 25996.0 10 23919.5 11 20302.0 12 16211.0 13 12501.0 14 9467.0 15 7458.0 16 6858.5 17 7267.5 18 8270.5 19 9696.5 20 11296.0 21 13000.0 22 14904.0 23 17008.5 24 19151.0 25 21440.5 26 24342.0 27 27822.0 28 31762.0 29 36948.0 30 42705.0 31 48676.0 32 55131.5 33 61227.0 34 66102.0 35 70158.5 36 74998.5 37 79914.5 38 83060.5 39 84254.0 40 84571.5 41 84718.0 42 85197.5 43 86215.5 44 87005.5 45 88024.0 46 88017.5 47 86820.0 48 84819.5 49 81429.5 50 77115.5 51 72037.0 52 66995.5 53 61500.5 54 55167.0 55 47741.0 56 39807.5 57 32677.0 58 26184.5 59 20282.0 60 15516.5 61 11719.0 62 8538.5 63 5899.0 64 3900.5 65 2480.5 66 1520.0 67 954.5 68 585.5 69 350.0 70 214.0 71 132.5 72 85.0 73 54.5 74 40.5 75 33.0 76 23.0 77 13.0 78 6.5 79 2.5 80 3.5 81 5.0 82 5.0 83 2.0 84 0.5 85 1.5 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.27444676124744 2 29.2950388506045 3 23.060775783189985 4 29.436662895368514 5 32.542139915606626 6 25.14513041701118 7 24.070844748522983 8 20.077120153024765 9 11.902564260115383 10-11 15.546636754528564 12-13 15.634963941135691 14-15 19.79926519795308 16-17 23.098043511074128 18-19 27.09216883482916 20-21 29.151227497438683 22-23 28.726221787061018 24-25 27.27640365090157 26-27 28.6980706270195 28-29 25.650582324945198 30-31 27.529179695900684 32-33 26.55066808079216 34-35 24.58780089682984 36-37 27.565028177875256 38-39 27.6985541724494 40-41 24.704496304617592 42-43 25.575612746898695 44-45 24.292013352265517 46-47 27.595383343329278 48-49 22.953597671501676 50-51 28.55953552923513 52-53 22.123038268212767 54-55 23.263210340926243 56-57 25.395986305779704 58-59 24.66853094356811 60-61 24.203803044733295 62-63 24.04042279502616 64-65 22.569883668587046 66-67 23.07351560235468 68-69 24.063414578761137 70-71 22.550665409270447 72-73 22.434203759632506 74-75 19.713576139036675 76-77 21.980061765581986 78-79 21.067453251709438 80-81 22.10595722626587 82-83 22.955050311432643 84-85 19.92574505401149 86-87 21.475678466332816 88-89 20.307255051513618 90-91 21.44173344357813 92-93 21.424602310599127 94-95 20.485228488573235 96-97 19.378917953560936 98-99 18.776456413455385 100-101 20.46826432570124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2994548.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.73646558744059 #Duplication Level Percentage of deduplicated Percentage of total 1 87.21246812898717 64.30739154988821 2 8.142540552109256 12.008043224298873 3 2.217740145456343 4.905849597519815 4 0.9108526868033324 2.686522311828069 5 0.4573504639965708 1.6861703374941566 6 0.26569241454919895 1.1754731749350604 7 0.1713157251261355 0.8842551249245505 8 0.11599645839966391 0.6842535090441443 9 0.08671287892012271 0.5754511091233377 >10 0.4005896149683754 4.954930386642048 >50 0.012659662450307951 0.6406859527977834 >100 0.005715286065554614 0.7238221947875615 >500 1.8299108420339526E-4 0.07421213679508337 >1k 1.3724331279413947E-4 0.18536167758726 >5k 0.0 0.0 >10k+ 4.5747770931379825E-5 4.507577712333977 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 133626 4.4623095038049145 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.3394021401560434E-5 0.0 10-11 0.0 0.0 0.0 6.678804280312087E-5 0.0 12-13 0.0 0.0 0.0 1.0018206420468131E-4 1.6697010700780217E-5 14-15 0.0 0.0 0.0 1.1687907490546152E-4 3.3394021401560434E-5 16-17 0.0 3.3394021401560434E-5 0.0 1.3357608560624174E-4 3.3394021401560434E-5 18-19 0.0 3.3394021401560434E-5 0.0 1.5027309630702196E-4 3.3394021401560434E-5 20-21 0.0 3.3394021401560434E-5 0.0 1.6697010700780218E-4 3.3394021401560434E-5 22-23 0.0 3.3394021401560434E-5 0.0 1.836671177085824E-4 3.3394021401560434E-5 24-25 0.0 6.678804280312087E-5 0.0 2.0036412840936262E-4 3.3394021401560434E-5 26-27 0.0 6.678804280312087E-5 0.0 2.3375814981092303E-4 3.3394021401560434E-5 28-29 0.0 6.678804280312087E-5 0.0 5.009103210234066E-4 3.3394021401560434E-5 30-31 0.0 6.678804280312087E-5 0.0 0.001619610037975681 6.678804280312087E-5 32-33 0.0 6.678804280312087E-5 0.0 0.004107464632391934 6.678804280312087E-5 34-35 0.0 6.678804280312087E-5 0.0 0.007964474104272165 6.678804280312087E-5 36-37 0.0 8.348505350390109E-5 0.0 0.014225853117064747 6.678804280312087E-5 38-39 0.0 1.0018206420468131E-4 0.0 0.02461139377295004 6.678804280312087E-5 40-41 0.0 1.0018206420468131E-4 0.0 0.03538096567495329 6.678804280312087E-5 42-43 0.0 1.0018206420468131E-4 0.0 0.046384295726767444 6.678804280312087E-5 44-45 0.0 1.0018206420468131E-4 0.0 0.06121124122906028 6.678804280312087E-5 46-47 0.0 1.0018206420468131E-4 0.0 0.07897686061469043 6.678804280312087E-5 48-49 0.0 1.0018206420468131E-4 0.0 0.09864593922020953 6.678804280312087E-5 50-51 0.0 1.0018206420468131E-4 0.0 0.12237239142601822 6.678804280312087E-5 52-53 0.0 1.0018206420468131E-4 0.0 0.1493213666970775 6.678804280312087E-5 54-55 0.0 1.0018206420468131E-4 0.0 0.17989359329020607 6.678804280312087E-5 56-57 1.6697010700780217E-5 1.0018206420468131E-4 0.0 0.21600922743599368 6.678804280312087E-5 58-59 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.25845302863737696 6.678804280312087E-5 60-61 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.3041026558933101 6.678804280312087E-5 62-63 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.351138135037408 6.678804280312087E-5 64-65 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.3989917677058441 6.678804280312087E-5 66-67 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.45763166928698423 6.678804280312087E-5 68-69 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.5267405965775135 6.678804280312087E-5 70-71 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.602745389287465 6.678804280312087E-5 72-73 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.6844939536784851 6.678804280312087E-5 74-75 3.3394021401560434E-5 1.0018206420468131E-4 0.0 0.7668937014868353 6.678804280312087E-5 76-77 6.678804280312087E-5 1.1687907490546152E-4 0.0 0.8618162073207709 6.678804280312087E-5 78-79 6.678804280312087E-5 1.3357608560624174E-4 0.0 0.9696788964478111 6.678804280312087E-5 80-81 6.678804280312087E-5 1.6697010700780218E-4 0.0 1.0880273082949414 6.678804280312087E-5 82-83 6.678804280312087E-5 1.6697010700780218E-4 0.0 1.2121361888338407 6.678804280312087E-5 84-85 6.678804280312087E-5 1.6697010700780218E-4 0.0 1.3377144063144089 6.678804280312087E-5 86-87 6.678804280312087E-5 1.6697010700780218E-4 0.0 1.470572520460517 6.678804280312087E-5 88-89 6.678804280312087E-5 1.6697010700780218E-4 0.0 1.6208289197568382 6.678804280312087E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2390 0.0 58.12785 1 GTATCAA 4410 0.0 45.10306 1 ATCAACG 5245 0.0 37.478714 3 TCAACGC 5470 0.0 36.02562 4 CAACGCA 5515 0.0 35.539074 5 TATCAAC 5575 0.0 34.568317 2 AACGCAG 6005 0.0 33.19146 6 ACGCAGA 6790 0.0 28.448986 7 CGCAGAG 7085 0.0 27.108526 8 GTACATG 4665 0.0 25.60455 1 TACATGG 4995 0.0 23.600847 2 GCAGAGT 7720 0.0 23.168001 9 GTGGTAT 1165 0.0 22.881807 1 ACATGGG 4900 0.0 22.630398 3 CAGAGTA 7205 0.0 20.976826 10-11 GAGTACT 5160 0.0 20.570375 12-13 AGAGTAC 6940 0.0 19.60003 10-11 GTACTAG 630 0.0 19.52916 1 GAGTACA 3680 0.0 19.502626 1 GCGGTAT 215 1.2425535E-5 19.074995 1 >>END_MODULE