FastQCFastQC Report
Sat 18 Jun 2016
SRR1931038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2994548
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77620.2592043941189121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69850.23325723948989965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.12833322424619675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.056.7680051
GTATCAA83500.042.465661
ATCAACG100250.034.8471073
TCAACGC102200.034.0415574
TATCAAC103050.033.9930762
CAACGCA102300.033.8977365
AACGCAG107150.032.5836756
ACGCAGA122650.027.9630937
CGCAGAG122850.027.5700478
GTGGTAT21350.027.3837381
GTACATG79150.024.3214591
GCAGAGT138450.024.052019
TGGTATC23950.023.0209622
TACATGG82650.022.1405722
ACATGGG79900.021.9504453
GAGTACT97050.021.359612-13
TATACCG2901.07320375E-1021.265445
CAGAGTA136400.020.3445810-11
AGAGTAC129900.020.23055610-11
GTACTAG8350.019.9235671
Produced by FastQC (version 0.11.5)