##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931038_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2994548 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.356391014603872 31.0 31.0 34.0 30.0 34.0 2 31.519902502815118 31.0 31.0 34.0 30.0 34.0 3 31.582829194923576 31.0 31.0 34.0 30.0 34.0 4 35.352746391108106 37.0 35.0 37.0 33.0 37.0 5 35.22770949071446 37.0 35.0 37.0 33.0 37.0 6 35.2589228825185 37.0 35.0 37.0 32.0 37.0 7 35.13948716133453 37.0 35.0 37.0 32.0 37.0 8 35.14447222084936 37.0 35.0 37.0 32.0 37.0 9 36.647615266143674 39.0 35.0 39.0 32.0 39.0 10-11 36.591194731224874 38.5 35.0 39.0 32.0 39.0 12-13 36.47011168296518 38.0 35.0 39.0 32.0 39.0 14-15 37.538890844294365 39.0 36.0 41.0 32.0 41.0 16-17 37.49563540140282 39.0 36.0 41.0 32.0 41.0 18-19 37.52124611126621 39.0 36.0 41.0 32.0 41.0 20-21 37.502039706827205 39.0 36.0 41.0 32.0 41.0 22-23 37.40525364762895 39.0 36.0 41.0 32.0 41.0 24-25 37.27827288124952 39.0 36.0 41.0 31.0 41.0 26-27 37.030637010994646 39.0 36.0 40.0 31.0 41.0 28-29 36.851864121062675 39.0 36.0 40.0 30.5 41.0 30-31 36.62450793909465 38.5 35.0 40.0 30.0 41.0 32-33 36.46084884930881 38.0 35.0 40.0 30.0 41.0 34-35 36.541246792504246 38.0 35.0 40.0 30.0 41.0 36-37 36.615521107025174 39.0 35.0 40.0 30.0 41.0 38-39 36.601362542861224 39.0 35.0 40.0 30.0 41.0 40-41 36.48786177413085 39.0 35.0 40.0 30.0 41.0 42-43 36.3512929163266 39.0 35.0 40.0 30.0 41.0 44-45 36.185512972241554 38.0 35.0 40.0 30.0 41.0 46-47 35.94517653415474 38.0 35.0 40.0 29.0 41.0 48-49 35.72314269131769 38.0 35.0 40.0 28.0 41.0 50-51 35.45962696206573 38.0 34.5 40.0 27.5 41.0 52-53 35.25007229805634 38.0 34.0 40.0 27.0 41.0 54-55 35.10038927410748 38.0 34.0 40.0 26.5 41.0 56-57 34.932054186474886 38.0 34.0 40.0 26.0 41.0 58-59 34.721575676863424 38.0 33.5 40.0 26.0 41.0 60-61 34.41598615216721 37.5 33.0 40.0 26.0 41.0 62-63 34.16067299639211 37.0 33.0 40.0 25.5 41.0 64-65 33.85040146292529 37.0 32.5 39.5 25.0 41.0 66-67 33.52510011527616 36.0 32.0 39.0 23.5 41.0 68-69 33.17782266305299 36.0 32.0 39.0 23.0 40.0 70-71 32.79721513897924 35.5 31.0 39.0 22.0 40.0 72-73 32.372202749797296 35.0 31.0 38.0 22.0 40.0 74-75 31.85670992750826 35.0 31.0 37.0 21.0 39.5 76-77 30.46053978764074 33.5 29.5 36.0 20.0 38.5 78-79 31.199054748830207 34.0 30.0 36.0 20.0 39.0 80-81 31.162331677435127 34.0 31.0 36.0 20.0 38.5 82-83 30.895641846448946 34.0 30.0 36.0 20.0 37.0 84-85 30.55953820075684 34.0 30.0 35.0 19.0 37.0 86-87 30.216012232897917 34.0 30.0 35.0 18.0 36.5 88-89 29.86518332649869 34.0 30.0 35.0 15.5 36.0 90-91 29.53119151872002 34.0 29.0 35.0 9.0 36.0 92-93 29.206968964932273 34.0 29.0 35.0 4.5 35.0 94-95 28.830071516636234 34.0 29.0 35.0 2.0 35.0 96-97 28.409217350999217 34.0 29.0 35.0 2.0 35.0 98-99 27.918831990671045 34.0 29.0 35.0 2.0 35.0 100-101 26.795931973706885 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25431119804281366 1101 2 0.2552333003004321 1101 3 0.17180726889511533 1101 4 0.11544814055676511 1101 5 0.26083047351662003 1101 6 0.25798545585143273 1101 7 0.2754650028019867 1101 8 0.1638550418171505 1101 9 0.2924141378568521 1101 10-11 0.304509058552938 1101 12-13 0.2962710492023177 1101 14-15 0.3887772919542627 1101 16-17 0.37068065036616105 1101 18-19 0.34410575034230106 1101 20-21 0.4202317240358582 1101 22-23 0.43177917832849033 1101 24-25 0.42882835939645503 1101 26-27 0.28428869799644474 1101 28-29 0.3695528449312704 1101 30-31 0.3230687703809636 1101 32-33 0.3546048628868945 1101 34-35 0.33061954793777204 1101 36-37 0.38360116401406685 1101 38-39 0.48343906440494067 1101 40-41 0.5803002431420907 1101 42-43 0.5205824413688873 1101 44-45 0.6752106536020435 1101 46-47 0.5535747404371847 1101 48-49 0.6716550628049802 1101 50-51 0.6445424576140724 1101 52-53 0.3661656328009073 1101 54-55 0.4188863788168291 1101 56-57 0.5056688166918732 1101 58-59 0.44943316495663765 1101 60-61 0.49535198012593185 1101 62-63 0.3255372561866352 1101 64-65 0.4600990769756024 1101 66-67 0.39034543489113105 1101 68-69 0.36742846772362725 1101 70-71 0.3180827539435427 1101 72-73 0.3756654967425206 1101 74-75 0.25925936923346526 1101 76-77 0.11164403782792931 1101 78-79 0.20953010621364143 1101 80-81 0.27772051803697906 1101 82-83 0.20108679581257505 1101 84-85 0.2386128499634914 1101 86-87 0.39324002832967864 1101 88-89 0.2958371073138579 1101 90-91 0.21291086133552994 1101 92-93 0.10299477602610807 1101 94-95 0.24308025101687036 1101 96-97 0.4683118865722591 1101 98-99 0.49074459380501523 1101 100-101 0.7020215194851147 1102 1 0.3143856878282456 1102 2 0.4873506350929908 1102 3 0.36153688857202937 1102 4 0.2247844976692832 1102 5 0.3282147846327561 1102 6 0.36620620029542295 1102 7 0.37593533277156865 1102 8 0.34877699388884054 1102 9 0.5452068090245064 1102 10-11 0.46752568505668535 1102 12-13 0.5184397672603893 1102 14-15 0.5552238226150976 1102 16-17 0.5130695215571066 1102 18-19 0.6162729055578211 1102 20-21 0.5449915172782127 1102 22-23 0.6101278253778517 1102 24-25 0.659237325317072 1102 26-27 0.5663603678144895 1102 28-29 0.6108521472612338 1102 30-31 0.5131905457872179 1102 32-33 0.5368164606343484 1102 34-35 0.545385369898753 1102 36-37 0.5993730390996959 1102 38-39 0.5791094874599949 1102 40-41 0.43585907067620866 1102 42-43 0.49871784604662395 1102 44-45 0.5267066792720598 1102 46-47 0.43075169446134964 1102 48-49 0.40081214903278095 1102 50-51 0.1321966973275721 1102 52-53 0.2603821198905436 1102 54-55 0.4748791199484472 1102 56-57 0.4287619775216456 1102 58-59 0.4337248311951285 1102 60-61 0.261131819285886 1102 62-63 0.2176116855992447 1102 64-65 0.22187215181049424 1102 66-67 0.2862207829982566 1102 68-69 0.3382663076097714 1102 70-71 0.3102859894129608 1102 72-73 0.3656544198283953 1102 74-75 0.472215505167366 1102 76-77 0.5437550142063117 1102 78-79 0.5993897950666742 1102 80-81 0.5327717642861565 1102 82-83 0.32770010960394913 1102 84-85 0.42949024903383304 1102 86-87 0.3195367487581322 1102 88-89 0.36413455735428 1102 90-91 0.3532222223872701 1102 92-93 0.3393686088182264 1102 94-95 0.5587291111266204 1102 96-97 0.6610763644886504 1102 98-99 0.5408814923889516 1102 100-101 0.6950321343972838 1103 1 0.33763705450579096 1103 2 0.4171919713092649 1103 3 0.3319977852756857 1103 4 0.2448622487027876 1103 5 0.396233340170177 1103 6 0.34658400844381276 1103 7 0.3442748195535543 1103 8 0.3575176107295732 1103 9 0.44708565068029316 1103 10-11 0.38261276825403456 1103 12-13 0.44344376190246493 1103 14-15 0.6014388567480395 1103 16-17 0.618934336069735 1103 18-19 0.5836328551063446 1103 20-21 0.6721639226306948 1103 22-23 0.6366968697914714 1103 24-25 0.4752232879748135 1103 26-27 0.4862601447944712 1103 28-29 0.49076003251472144 1103 30-31 0.5490883543435672 1103 32-33 0.5560213398771694 1103 34-35 0.6214263566263369 1103 36-37 0.5089704641704103 1103 38-39 0.4572682294367141 1103 40-41 0.46805886057503443 1103 42-43 0.609219845067507 1103 44-45 0.6554420945788522 1103 46-47 0.6437774573529964 1103 48-49 0.4441569903350029 1103 50-51 0.3549536633034194 1103 52-53 0.3670369386187815 1103 54-55 0.45083094966449977 1103 56-57 0.42425058174270447 1103 58-59 0.5406023299712359 1103 60-61 0.46514626459158137 1103 62-63 0.24396994889885804 1103 64-65 0.2719288417310821 1103 66-67 0.3817594477338986 1103 68-69 0.15367687182321532 1103 70-71 0.21778282769625434 1103 72-73 0.27313935917783283 1103 74-75 0.39822623460601037 1103 76-77 0.4176875098547015 1103 78-79 0.5300517327570624 1103 80-81 0.3993715419460777 1103 82-83 0.3665514035280175 1103 84-85 0.41092748725728967 1103 86-87 0.42916630095025354 1103 88-89 0.4622085449249944 1103 90-91 0.33875141897125616 1103 92-93 0.29022346222336637 1103 94-95 0.473050412937976 1103 96-97 0.6568169711302971 1103 98-99 0.6132070370201532 1103 100-101 0.8521537313022023 1104 1 0.3460989843775195 1104 2 0.40225973602213116 1104 3 0.4117475003455624 1104 4 0.2550784987089756 1104 5 0.34406947937112164 1104 6 0.3209402972763655 1104 7 0.38322901466512604 1104 8 0.35369497004171535 1104 9 0.3847702779784967 1104 10-11 0.3810316328549135 1104 12-13 0.42948878333614005 1104 14-15 0.6597457244299463 1104 16-17 0.5459280016318644 1104 18-19 0.47504015014221324 1104 20-21 0.5409372097924248 1104 22-23 0.6013111049169595 1104 24-25 0.4912409173561798 1104 26-27 0.5538108508109048 1104 28-29 0.6026181975152411 1104 30-31 0.568104347541535 1104 32-33 0.6075163777221562 1104 34-35 0.6152676806483797 1104 36-37 0.6853801466815597 1104 38-39 0.5881600153671869 1104 40-41 0.438344277265756 1104 42-43 0.5185242362077105 1104 44-45 0.6988537349021939 1104 46-47 0.3706274002020322 1104 48-49 0.4275016700174632 1104 50-51 0.38355605011894767 1104 52-53 0.371078018862697 1104 54-55 0.4421192420465232 1104 56-57 0.46588903716578756 1104 58-59 0.4841263754347054 1104 60-61 0.3688614276573787 1104 62-63 0.24715994987198542 1104 64-65 0.2350146184811166 1104 66-67 0.11369772304485082 1104 68-69 0.17874763015794315 1104 70-71 0.3250676786501714 1104 72-73 0.21229914931092964 1104 74-75 0.3035521187965493 1104 76-77 0.28891682458744583 1104 78-79 0.4149090367820385 1104 80-81 0.37900512015712806 1104 82-83 0.29197702563232397 1104 84-85 0.29570046680994366 1104 86-87 0.2448737234830709 1104 88-89 0.4217436222560984 1104 90-91 0.4101482693182028 1104 92-93 0.5100725152327357 1104 94-95 0.6531240865490773 1104 96-97 0.7249098072200155 1104 98-99 0.7698804633894234 1104 100-101 1.108427039081164 1105 1 0.2517048429208373 1105 2 0.3205325491309665 1105 3 0.28188727404754843 1105 4 0.1521590219552209 1105 5 0.2611004808427353 1105 6 0.2376732877483363 1105 7 0.30001296244043374 1105 8 0.1997240081700724 1105 9 0.3322959539162227 1105 10-11 0.46339598431517004 1105 12-13 0.37691452744164167 1105 14-15 0.35980925252328433 1105 16-17 0.38904424495635936 1105 18-19 0.34347939733827104 1105 20-21 0.3942612716724412 1105 22-23 0.4777729549486267 1105 24-25 0.5393914051781721 1105 26-27 0.4782674417316244 1105 28-29 0.4933339156424381 1105 30-31 0.3359714255149271 1105 32-33 0.4446088531327774 1105 34-35 0.5103958650337148 1105 36-37 0.4353844096466233 1105 38-39 0.4821879582109432 1105 40-41 0.3613040717551499 1105 42-43 0.36006804025566197 1105 44-45 0.45307337634519484 1105 46-47 0.39461520375984094 1105 48-49 0.275098884457293 1105 50-51 0.07679348061775215 1105 52-53 0.10234298137437747 1105 54-55 0.15158697021131928 1105 56-57 0.20696397453985327 1105 58-59 0.3359971627825402 1105 60-61 0.19987045364322853 1105 62-63 0.08159332646945217 1105 64-65 0.12321389841674346 1105 66-67 0.16858607499841582 1105 68-69 0.05128229797779227 1105 70-71 0.05381174172777037 1105 72-73 0.16372177015011857 1105 74-75 0.22810675791375346 1105 76-77 0.33702537672985144 1105 78-79 0.4092810306481276 1105 80-81 0.3665823442649696 1105 82-83 0.4573453715899838 1105 84-85 0.2415939228214974 1105 86-87 0.2400811593631218 1105 88-89 0.2901055412486606 1105 90-91 0.3610923475442043 1105 92-93 0.32701782119267975 1105 94-95 0.4238117603144751 1105 96-97 0.3392695951265985 1105 98-99 0.35750015852595496 1105 100-101 0.44495581171602083 1106 1 0.2017528062347509 1106 2 0.21400957809414578 1106 3 0.2831941206866837 1106 4 0.08356870717391018 1106 5 0.14855463414054526 1106 6 0.046145603656654544 1106 7 0.2044545358274732 1106 8 0.1490203016904843 1106 9 0.21010192674665973 1106 10-11 0.12566208137039325 1106 12-13 0.17822535945742857 1106 14-15 0.25134395563652134 1106 16-17 0.2813986270266824 1106 18-19 0.2270932139451176 1106 20-21 0.20502438604005846 1106 22-23 0.20980763050129525 1106 24-25 0.1957576577225737 1106 26-27 0.1176137566858273 1106 28-29 0.2295922973257305 1106 30-31 0.2284012521530272 1106 32-33 0.2381228211839428 1106 34-35 0.2749328733205232 1106 36-37 0.271292970433926 1106 38-39 0.2961511714000338 1106 40-41 0.30268413828913054 1106 42-43 0.281790186934181 1106 44-45 0.23359789156675248 1106 46-47 0.17148021132015856 1106 48-49 0.1460740010345205 1106 50-51 -0.08012051336810089 1106 52-53 -0.023741943234590224 1106 54-55 0.11662966529352303 1106 56-57 0.15729775479054098 1106 58-59 0.11441696292681058 1106 60-61 0.1103333164760869 1106 62-63 -0.0581148448216382 1106 64-65 0.11341007015514748 1106 66-67 -0.009644033114341255 1106 68-69 -0.024821542299420685 1106 70-71 0.04379793765205875 1106 72-73 0.026259983170682233 1106 74-75 0.2969191434333176 1106 76-77 0.16415371656470157 1106 78-79 0.2135508561124233 1106 80-81 0.15822179735232922 1106 82-83 0.29023981779909747 1106 84-85 0.31923173655094317 1106 86-87 0.20286487951592136 1106 88-89 0.059456825035631766 1106 90-91 0.31081156685452527 1106 92-93 0.3960485101790425 1106 94-95 0.43313040366847133 1106 96-97 0.3940506789824916 1106 98-99 0.41217357772928764 1106 100-101 0.7363487068006869 1107 1 0.1471445785843848 1107 2 0.19838153134078595 1107 3 0.1303289624180053 1107 4 0.08307081919684123 1107 5 0.13302967994800952 1107 6 0.18672016500762822 1107 7 0.15636480170613254 1107 8 0.19194293848007504 1107 9 0.19314231684105465 1107 10-11 0.17193237906832337 1107 12-13 0.19025066485195907 1107 14-15 0.20725151283178178 1107 16-17 0.1219784510074291 1107 18-19 0.24435503232901112 1107 20-21 0.23979038850917078 1107 22-23 0.29762413202676896 1107 24-25 0.2577280196913563 1107 26-27 0.2140494835796929 1107 28-29 0.23375488038151104 1107 30-31 0.3616643418154766 1107 32-33 0.36299987103223685 1107 34-35 0.36730837334398814 1107 36-37 0.44354291746399355 1107 38-39 0.4585442914149027 1107 40-41 0.42287248937957855 1107 42-43 0.3503720221035067 1107 44-45 0.2857379851276747 1107 46-47 0.33558982247269853 1107 48-49 0.35786662161010696 1107 50-51 0.37230919174201205 1107 52-53 0.3156508926375281 1107 54-55 0.29125000484703634 1107 56-57 0.3922303842929651 1107 58-59 0.46072843873903935 1107 60-61 0.5441774456296571 1107 62-63 0.3677341125983915 1107 64-65 0.3092910903434287 1107 66-67 0.378410013307942 1107 68-69 0.208793760642493 1107 70-71 0.2267184065523793 1107 72-73 0.36602709413028833 1107 74-75 0.35902593122424875 1107 76-77 0.441612911056378 1107 78-79 0.39605650018466676 1107 80-81 0.27247687701735046 1107 82-83 0.44693569926111465 1107 84-85 0.4177776208897299 1107 86-87 0.5093790922490911 1107 88-89 0.4943291797541569 1107 90-91 0.4694053332695596 1107 92-93 0.3471283900261355 1107 94-95 0.5323198801369564 1107 96-97 0.645664617567995 1107 98-99 0.6446158346041351 1107 100-101 0.7296924128820272 1108 1 0.005145979247672017 1108 2 0.0768103071078734 1108 3 0.0927211337703433 1108 4 0.02821322222945355 1108 5 -0.03988136783705443 1108 6 0.04288918976814671 1108 7 -0.05125799854479851 1108 8 0.02446552839852245 1108 9 0.08547928683785244 1108 10-11 0.0806390915539339 1108 12-13 0.052067533272513344 1108 14-15 0.07465025937987235 1108 16-17 0.09008884867037636 1108 18-19 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-0.9722872506684617 2316 90-91 -1.0642464685819277 2316 92-93 -0.9668000535437287 2316 94-95 -1.2389057685867648 2316 96-97 -0.974031951362484 2316 98-99 -1.138060601796969 2316 100-101 -1.511964066062248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 22.0 4 30.0 5 75.0 6 171.0 7 354.0 8 963.0 9 1770.0 10 3190.0 11 5086.0 12 7200.0 13 9610.0 14 11931.0 15 15262.0 16 18274.0 17 21895.0 18 18948.0 19 15212.0 20 14799.0 21 15391.0 22 17290.0 23 20031.0 24 24732.0 25 31026.0 26 40399.0 27 51451.0 28 65377.0 29 82135.0 30 100807.0 31 122160.0 32 147915.0 33 179836.0 34 223253.0 35 279401.0 36 367089.0 37 506407.0 38 484335.0 39 90697.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.21489497203607 21.602508421573255 14.991552545040014 23.191044061350667 2 14.98120448901458 21.739673489780024 40.67778723267828 22.601334788527126 3 18.367319677794562 26.31085057306978 31.116915852364695 24.204913896770964 4 12.447609059630919 18.217779718028105 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20.795982535463466 28.044977409609633 30.42174795305634 20.737292101870565 26-27 20.735901048990108 28.215718304337205 30.272372085505477 20.77600856116721 28-29 20.7367771098218 28.40614691662364 30.214219737108376 20.642856236446175 30-31 21.0197766320614 28.101499427921063 30.166327026104568 20.712396913912972 32-33 20.89427508032136 28.215367791494135 30.067142944462944 20.82321418372156 34-35 20.98456329293706 28.227853872051963 30.175164434270464 20.612418400740516 36-37 21.073824674100578 28.06320744840451 30.206505515657682 20.65646236183723 38-39 20.909101079113807 28.182420641831357 30.227893060721673 20.680585218333164 40-41 20.947679307422224 28.121846295233855 30.19940931589778 20.731065081446147 42-43 20.95742257739589 28.17009527702744 29.90630936445467 20.966172781122 44-45 20.894963868281366 28.44450262864181 29.83069289141248 20.829840611664345 46-47 21.04197159367483 28.438269971136126 29.566140181399042 20.95361825379 48-49 21.12881921425673 28.519521680829996 29.346060011989707 21.005599092923568 50-51 21.15752615833667 28.592504413463903 29.129045786770934 21.120923641428497 52-53 21.201399959981085 28.64627239843194 29.104350243414974 21.047977398172 54-55 21.100577150453237 28.707957862672927 29.158455855338307 21.033009131535525 56-57 21.107750334964408 28.72328132868992 29.053603655954486 21.115364680391192 58-59 21.140736898481276 28.638930365436543 29.178961417597794 21.041371318484387 60-61 21.059557189600394 28.811330457408474 29.18372171247483 20.9453906405163 62-63 21.068254572392547 28.71005924686618 29.208313374045257 21.013372806696008 64-65 20.858088670122246 28.585352495630868 29.20016919134138 21.356389642905505 66-67 20.901690730803026 28.64635236283643 29.123913213426412 21.32804369293413 68-69 20.94961784986544 28.670659215887078 29.136296671504887 21.24342626274259 70-71 20.956707388919835 28.5212151602468 29.133705921419086 21.388371529414286 72-73 21.013333806074378 28.672374234297486 29.05367221953425 21.260619740093887 74-75 20.924137928725184 28.831598567719645 28.941763676420635 21.302499827134536 76-77 20.884092344631703 28.71742358644865 28.978545224858927 21.41993884406072 78-79 20.78025106787923 28.747804838380837 29.014188128752984 21.45775596498695 80-81 20.86717260835358 28.89542929350273 28.838275425873956 21.399122672269737 82-83 20.95765370934111 28.751935183540223 29.051980465833243 21.23843064128543 84-85 21.048051325275132 28.78851833398563 28.695699651499996 21.467730689239247 86-87 20.84721968056615 28.95777593145944 28.830578103940894 21.364426284033517 88-89 20.861552682258786 28.728184930809235 28.7699274715007 21.640334915431282 90-91 20.909083470855926 28.684293269689164 28.942128300354348 21.46449495910056 92-93 20.898783897073805 28.718733195501656 28.83439824448312 21.548084662941424 94-95 20.647843651928678 28.63265566806842 28.880261882518543 21.839238797484363 96-97 20.668194331832385 28.519329127467653 28.916217071825194 21.896259468874767 98-99 20.512010493737286 28.64176830693647 28.85016369749291 21.996057501833334 100-101 20.545387818128145 28.508359191437236 28.805515890879025 22.140737099555594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 153.5 2 145.5 3 216.0 4 223.5 5 233.5 6 400.5 7 852.5 8 1439.0 9 1879.5 10 1835.0 11 1654.0 12 1773.0 13 1980.5 14 2272.0 15 2598.0 16 2893.0 17 3287.0 18 3763.0 19 4167.5 20 4775.5 21 5558.5 22 6563.5 23 8097.5 24 10303.0 25 13440.0 26 17396.5 27 22495.0 28 28763.0 29 37324.5 30 46977.5 31 56492.0 32 68221.5 33 78385.0 34 85346.5 35 91607.5 36 99765.5 37 108805.0 38 114033.5 39 115316.0 40 114951.0 41 115425.5 42 116751.5 43 119293.0 44 121353.5 45 123828.5 46 125368.0 47 124591.5 48 122143.0 49 116603.5 50 109851.5 51 102764.5 52 95527.0 53 87110.0 54 77664.0 55 66092.5 56 53525.5 57 42107.5 58 32442.0 59 24390.0 60 17585.5 61 12570.0 62 8716.5 63 5516.0 64 3140.0 65 1750.5 66 891.0 67 480.5 68 244.0 69 122.5 70 74.0 71 46.0 72 24.0 73 18.5 74 14.0 75 7.5 76 10.0 77 10.0 78 4.5 79 2.0 80 1.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005176073317241868 2 0.007213108622737055 3 0.0020370353054951864 4 0.17508485420838135 5 0.002070429326896747 6 5.343043424249671E-4 7 0.0 8 7.680624922358901E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.838491819132637E-4 24-25 7.513654815351098E-4 26-27 0.003222523065250582 28-29 2.003641284093626E-4 30-31 0.00687916840872145 32-33 0.021271991632794 34-35 0.013975397956553042 36-37 9.183355885429119E-4 38-39 2.504551605117033E-4 40-41 0.0018032771556842637 42-43 0.011086815105318065 44-45 0.007547048836752659 46-47 0.01070278385920012 48-49 0.010452328698688416 50-51 0.18976152661436718 52-53 0.115626799102903 54-55 0.01763204330002391 56-57 0.006678804280312087 58-59 0.0184835908457637 60-61 0.12496042808463916 62-63 0.24100465245506167 64-65 0.2470322733180433 66-67 0.2572508438669208 68-69 0.2527092569563086 70-71 0.23896761714956646 72-73 0.16334685568573287 74-75 0.2712095448127731 76-77 0.20989812151950812 78-79 0.005960832820178538 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 3.339402140156044E-5 90-91 0.006394955098398823 92-93 0.01863386394207072 94-95 0.0025212486158178128 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2994548.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.62125302435695 #Duplication Level Percentage of deduplicated Percentage of total 1 70.68923145647402 36.490667031119784 2 16.115501347165463 16.638047453127868 3 5.6639038756236255 8.771334452076095 4 2.583837447642163 5.335237066341789 5 1.3457371914291956 3.473432003152699 6 0.8437912501840484 2.6134536975293545 7 0.5402485555298248 1.952181516673391 8 0.37745743152342853 1.558786046287583 9 0.27883957975371176 1.2954643649704545 >10 1.4230231223783145 13.104601155711299 >50 0.09515565359512596 3.346802833359383 >100 0.04048478972150377 3.7394284108198597 >500 0.00213985735657 0.7202464829427508 >1k 5.187532983976809E-4 0.46668571188426144 >5k 1.2968832459942021E-4 0.49363177400352914 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7762 0.2592043941189121 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6985 0.23325723948989965 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3843 0.12833322424619675 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6697010700780217E-5 0.0 12-13 0.0 0.0 0.0 3.3394021401560434E-5 0.0 14-15 0.0 0.0 0.0 3.3394021401560434E-5 0.0 16-17 0.0 0.0 0.0 3.3394021401560434E-5 0.0 18-19 0.0 0.0 0.0 3.3394021401560434E-5 0.0 20-21 0.0 0.0 0.0 3.3394021401560434E-5 0.0 22-23 0.0 0.0 0.0 5.009103210234065E-5 0.0 24-25 0.0 0.0 0.0 6.678804280312087E-5 0.0 26-27 0.0 0.0 0.0 2.3375814981092303E-4 0.0 28-29 0.0 0.0 0.0 6.010923852280878E-4 0.0 30-31 0.0 0.0 0.0 0.002621430680022494 0.0 32-33 0.0 0.0 0.0 0.007263199654839394 0.0 34-35 0.0 0.0 0.0 0.014826945502292833 0.0 36-37 0.0 0.0 0.0 0.027650249720492043 0.0 38-39 0.0 0.0 0.0 0.04837124000016029 0.0 40-41 0.0 0.0 0.0 0.07253181448418927 0.0 42-43 0.0 0.0 0.0 0.09695954113943073 1.6697010700780217E-5 44-45 0.0 0.0 0.0 0.1263462799728039 3.3394021401560434E-5 46-47 0.0 0.0 0.0 0.16242852009718994 3.3394021401560434E-5 48-49 0.0 0.0 0.0 0.20326940827129836 3.3394021401560434E-5 50-51 0.0 0.0 0.0 0.24992085616927828 3.3394021401560434E-5 52-53 0.0 0.0 0.0 0.2966557891207621 3.3394021401560434E-5 54-55 0.0 0.0 0.0 0.3499025562455502 3.3394021401560434E-5 56-57 0.0 0.0 0.0 0.4154884142782149 3.3394021401560434E-5 58-59 0.0 0.0 0.0 0.48913892847935647 3.3394021401560434E-5 60-61 0.0 0.0 0.0 0.5719727985659271 3.3394021401560434E-5 62-63 0.0 0.0 0.0 0.6569271890114969 3.3394021401560434E-5 64-65 0.0 0.0 0.0 0.7453211636614274 3.3394021401560434E-5 66-67 0.0 0.0 0.0 0.8518814859538067 3.3394021401560434E-5 68-69 0.0 0.0 0.0 0.9766582469207372 3.3394021401560434E-5 70-71 0.0 0.0 0.0 1.1161951653471576 3.3394021401560434E-5 72-73 0.0 0.0 0.0 1.2665517467076834 3.3394021401560434E-5 74-75 0.0 0.0 0.0 1.4170419041538156 3.3394021401560434E-5 76-77 0.0 0.0 0.0 1.5871510491733645 3.3394021401560434E-5 78-79 0.0 0.0 0.0 1.7842258664746733 3.3394021401560434E-5 80-81 0.0 0.0 0.0 2.008032597907931 3.3394021401560434E-5 82-83 0.0 0.0 0.0 2.2443119963346723 3.3394021401560434E-5 84-85 0.0 0.0 0.0 2.48523316373623 3.3394021401560434E-5 86-87 0.0 0.0 0.0 2.742767856785064 3.3394021401560434E-5 88-89 0.0 0.0 0.0 3.034831967963112 3.3394021401560434E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4220 0.0 56.768005 1 GTATCAA 8350 0.0 42.46566 1 ATCAACG 10025 0.0 34.847107 3 TCAACGC 10220 0.0 34.041557 4 TATCAAC 10305 0.0 33.993076 2 CAACGCA 10230 0.0 33.897736 5 AACGCAG 10715 0.0 32.583675 6 ACGCAGA 12265 0.0 27.963093 7 CGCAGAG 12285 0.0 27.570047 8 GTGGTAT 2135 0.0 27.383738 1 GTACATG 7915 0.0 24.321459 1 GCAGAGT 13845 0.0 24.05201 9 TGGTATC 2395 0.0 23.020962 2 TACATGG 8265 0.0 22.140572 2 ACATGGG 7990 0.0 21.950445 3 GAGTACT 9705 0.0 21.3596 12-13 TATACCG 290 1.07320375E-10 21.26544 5 CAGAGTA 13640 0.0 20.34458 10-11 AGAGTAC 12990 0.0 20.230556 10-11 GTACTAG 835 0.0 19.923567 1 >>END_MODULE