FastQCFastQC Report
Sat 18 Jun 2016
SRR1931037_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931037_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016750
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1320854.378387337366371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.055.4128531
GTATCAA47550.043.411371
ATCAACG54250.036.306623
TCAACGC55800.035.292844
TATCAAC57000.034.413512
CAACGCA57000.034.3705445
AACGCAG62000.031.4012666
GTGGTAT13650.029.2692051
ACGCAGA69050.027.3240177
CGCAGAG70000.027.055098
TGGTATC13800.025.2368342
GTACATG48250.024.4162791
GCAGAGT77800.023.0426039
TACATGG49300.022.7510912
ACATGGG49150.021.3181063
GTGTAGC10750.020.9649181
GAGTACT49550.020.93875112-13
CAGAGTA74200.019.81700510-11
AGAGTAC67000.019.1776310-11
GTACTTT56100.018.41810614-15
Produced by FastQC (version 0.11.5)