##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931037_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3016750 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.96769868235684 28.0 2.0 31.0 2.0 33.0 2 19.698710532858208 27.0 2.0 31.0 2.0 33.0 3 20.326408220767384 27.0 2.0 31.0 2.0 33.0 4 21.852321869561614 30.0 2.0 35.0 2.0 37.0 5 21.21186177177426 30.0 2.0 35.0 2.0 37.0 6 22.163905527471616 30.0 2.0 35.0 2.0 37.0 7 22.32977542056849 30.0 2.0 35.0 2.0 37.0 8 22.754688986492084 31.0 2.0 35.0 2.0 37.0 9 24.014751305212563 32.0 2.0 37.0 2.0 39.0 10-11 24.364407392060993 32.0 2.0 37.0 2.0 39.0 12-13 24.255852158780144 32.0 2.0 37.0 2.0 39.0 14-15 24.212412695781886 32.0 2.0 38.0 2.0 40.0 16-17 23.062528880417666 31.0 2.0 38.0 2.0 40.0 18-19 22.028788431258803 28.5 2.0 37.0 2.0 40.0 20-21 21.689371343333057 27.0 2.0 37.0 2.0 40.0 22-23 21.31959691721223 27.0 2.0 37.0 2.0 39.5 24-25 21.157289135659234 27.0 2.0 37.0 2.0 39.0 26-27 20.873967680450818 26.5 2.0 37.0 2.0 39.0 28-29 20.671226816938756 25.0 2.0 36.5 2.0 39.0 30-31 20.639996850915722 25.0 2.0 36.0 2.0 39.0 32-33 20.50400546946217 25.0 2.0 36.0 2.0 39.0 34-35 20.178664622524238 25.0 2.0 36.0 2.0 39.0 36-37 19.725402668434572 24.0 2.0 36.0 2.0 39.0 38-39 19.594043258473523 24.0 2.0 35.0 2.0 39.0 40-41 19.450632634457612 24.0 2.0 35.0 2.0 39.0 42-43 19.305390900803847 23.5 2.0 35.0 2.0 38.5 44-45 18.922089500290046 21.5 2.0 35.0 2.0 38.0 46-47 18.58390221264606 19.0 2.0 35.0 2.0 38.0 48-49 18.335507251180907 18.0 2.0 34.5 2.0 38.0 50-51 16.107266594845445 10.0 2.0 32.5 2.0 37.5 52-53 17.956886218612745 17.5 2.0 33.5 2.0 38.0 54-55 18.724664291041684 21.0 2.0 35.0 2.0 39.0 56-57 18.691939172951024 20.0 2.0 35.0 2.0 39.0 58-59 18.417223170630646 18.5 2.0 35.0 2.0 39.0 60-61 18.11955829949449 17.0 2.0 35.0 2.0 39.0 62-63 17.682764398773514 12.5 2.0 34.0 2.0 38.0 64-65 17.322286235186873 9.5 2.0 34.0 2.0 38.0 66-67 16.957399353609016 8.0 2.0 34.0 2.0 38.0 68-69 16.519422060164082 7.0 2.0 33.0 2.0 37.5 70-71 16.163665036877433 2.0 2.0 32.5 2.0 37.0 72-73 15.757255655921107 2.0 2.0 32.0 2.0 36.0 74-75 15.307948288721306 2.0 2.0 31.5 2.0 36.0 76-77 14.892471699676804 2.0 2.0 31.0 2.0 35.5 78-79 14.507893096875778 2.0 2.0 31.0 2.0 35.0 80-81 14.067730504682192 2.0 2.0 30.0 2.0 34.5 82-83 13.606218447004228 2.0 2.0 30.0 2.0 34.0 84-85 13.20179746415845 2.0 2.0 29.0 2.0 34.0 86-87 12.719296428275461 2.0 2.0 29.0 2.0 34.0 88-89 12.29178569652772 2.0 2.0 29.0 2.0 34.0 90-91 11.806660810474849 2.0 2.0 27.5 2.0 34.0 92-93 11.26343946299826 2.0 2.0 26.0 2.0 34.0 94-95 10.694668931797464 2.0 2.0 25.5 2.0 33.0 96-97 10.061712438882903 2.0 2.0 24.5 2.0 33.0 98-99 9.393746747327423 2.0 2.0 19.0 2.0 32.0 100-101 8.48721571227314 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.884349098189599 1101 2 9.352772765173231 1101 3 8.749575442928084 1101 4 10.705745061668772 1101 5 11.653256810258707 1101 6 10.84816790444943 1101 7 10.438075334645095 1101 8 9.892901178473121 1101 9 9.745967582816508 1101 10-11 9.2069483403043 1101 12-13 9.284179221141969 1101 14-15 9.758864764730504 1101 16-17 10.74467672752623 1101 18-19 11.72310720283124 1101 20-21 11.664861051812366 1101 22-23 11.989649820980635 1101 24-25 11.757795566777634 1101 26-27 11.70615704018467 1101 28-29 11.658503359249792 1101 30-31 11.195715052385665 1101 32-33 11.19958062868212 1101 34-35 11.108850389540098 1101 36-37 11.513069665524004 1101 38-39 11.372237268360333 1101 40-41 10.870525046177494 1101 42-43 10.605374627363346 1101 44-45 10.414793273521642 1101 46-47 10.275658502275625 1101 48-49 10.41498080255035 1101 50-51 12.06932726040833 1101 52-53 11.297536141829529 1101 54-55 11.034200203171874 1101 56-57 10.970193351556944 1101 58-59 10.836997728650147 1101 60-61 10.33970451867631 1101 62-63 10.415893978313129 1101 64-65 10.232180150038609 1101 66-67 10.078795789654617 1101 68-69 10.072497701028215 1101 70-71 9.670196952428515 1101 72-73 9.452640507801533 1101 74-75 9.223126018085908 1101 76-77 9.22034600926353 1101 78-79 8.70984165846666 1101 80-81 8.703652446320598 1101 82-83 8.436897499667333 1101 84-85 8.045580018569353 1101 86-87 7.827495414705643 1101 88-89 7.531800770903326 1101 90-91 7.23246495863396 1101 92-93 7.060062602856073 1101 94-95 6.887792148724261 1101 96-97 6.769669828162563 1101 98-99 6.351557162943445 1101 100-101 5.717332859486348 1102 1 7.624506759525644 1102 2 8.842779017117213 1102 3 8.372564288253326 1102 4 10.142712775215308 1102 5 10.917828676926902 1102 6 10.033233664289476 1102 7 9.707561895046052 1102 8 9.109578785174868 1102 9 8.78773223301452 1102 10-11 8.11620667874356 1102 12-13 8.532840462522145 1102 14-15 8.911658894768856 1102 16-17 9.927033294303964 1102 18-19 10.830798446260712 1102 20-21 10.880731627209663 1102 22-23 10.977681821363447 1102 24-25 10.52626355963315 1102 26-27 10.810558939277673 1102 28-29 10.567440310319228 1102 30-31 10.68280551624662 1102 32-33 10.553834707269367 1102 34-35 10.29010724873405 1102 36-37 10.56339308139479 1102 38-39 10.45460845074754 1102 40-41 10.061685203089848 1102 42-43 9.592712880418322 1102 44-45 9.425105704279542 1102 46-47 9.461712870262343 1102 48-49 9.265850145243224 1102 50-51 10.983679684883084 1102 52-53 10.211276791220303 1102 54-55 9.905977773360611 1102 56-57 9.736668680304128 1102 58-59 9.881958048050627 1102 60-61 9.666418367928518 1102 62-63 9.490510981728288 1102 64-65 9.362396128176567 1102 66-67 9.158260286206684 1102 68-69 8.920814045232579 1102 70-71 8.750546239488447 1102 72-73 8.589379728919582 1102 74-75 8.347896199329865 1102 76-77 8.188532196524339 1102 78-79 7.8219637201310075 1102 80-81 7.538691107468358 1102 82-83 7.423042630068737 1102 84-85 7.008995535665456 1102 86-87 6.656664790374661 1102 88-89 6.391356830867894 1102 90-91 6.395759441839532 1102 92-93 6.272601859550905 1102 94-95 5.775534853662672 1102 96-97 5.563892657432479 1102 98-99 5.233053957391801 1102 100-101 4.559377650868026 1103 1 7.763443151984397 1103 2 9.145568452177912 1103 3 8.51814107335072 1103 4 10.510013261925323 1103 5 11.180375922140033 1103 6 10.484171822886214 1103 7 10.023909914171039 1103 8 9.588114893906827 1103 9 9.526454808305349 1103 10-11 8.87582007032129 1103 12-13 8.63665697632112 1103 14-15 9.333645370730778 1103 16-17 10.255711470848258 1103 18-19 11.13043458912983 1103 20-21 11.602077264910172 1103 22-23 11.65975511200148 1103 24-25 11.464824545563328 1103 26-27 11.335978448152314 1103 28-29 11.057659605785528 1103 30-31 10.947293060272312 1103 32-33 11.01564882124659 1103 34-35 11.13426253805157 1103 36-37 11.364532179069986 1103 38-39 11.012006913144543 1103 40-41 10.539920516446468 1103 42-43 10.20531286447402 1103 44-45 10.441384120397188 1103 46-47 10.391161892447407 1103 48-49 10.218820282384652 1103 50-51 11.69382925469357 1103 52-53 10.929137474821996 1103 54-55 10.523535924066966 1103 56-57 10.244118552851923 1103 58-59 10.114408687151368 1103 60-61 10.090146247765727 1103 62-63 10.032436930669732 1103 64-65 9.57095525393827 1103 66-67 9.144675639342296 1103 68-69 9.015215449148744 1103 70-71 8.920886977382533 1103 72-73 8.805323008089985 1103 74-75 8.567221101262193 1103 76-77 8.351402109423482 1103 78-79 7.847928094838439 1103 80-81 7.6195333140778825 1103 82-83 7.50260540871073 1103 84-85 7.043701680149439 1103 86-87 6.931025740736777 1103 88-89 6.6188129176048935 1103 90-91 6.3561394885686 1103 92-93 6.164051625803362 1103 94-95 5.942253782205627 1103 96-97 5.4547639317296515 1103 98-99 4.874276517351573 1103 100-101 4.244146550486384 1104 1 7.794547784029469 1104 2 9.415419604160725 1104 3 8.589585929435074 1104 4 10.752832824807584 1104 5 11.486288191688669 1104 6 10.519128089190442 1104 7 10.351261559391023 1104 8 9.673859243601019 1104 9 9.457861959148236 1104 10-11 8.798229479094331 1104 12-13 8.569261049364059 1104 14-15 9.228164957726854 1104 16-17 10.236992572192062 1104 18-19 11.131011128577843 1104 20-21 11.387578284266986 1104 22-23 11.390989081793446 1104 24-25 11.197815535764349 1104 26-27 11.387270729538635 1104 28-29 11.338046303727083 1104 30-31 10.877983373094285 1104 32-33 10.957678108573685 1104 34-35 10.845497384709613 1104 36-37 10.756223872956038 1104 38-39 10.333048410831804 1104 40-41 9.937688892271272 1104 42-43 9.920431131786646 1104 44-45 9.861965263392126 1104 46-47 10.030728282170536 1104 48-49 9.904943876658717 1104 50-51 11.484571741047342 1104 52-53 10.898800100597473 1104 54-55 10.427651714232912 1104 56-57 10.348432733968615 1104 58-59 10.037036216361969 1104 60-61 9.718733178076793 1104 62-63 9.775881260634435 1104 64-65 9.66810982350663 1104 66-67 9.324552658874488 1104 68-69 9.05284944524669 1104 70-71 8.809823119610211 1104 72-73 8.540342847185059 1104 74-75 8.261791108057905 1104 76-77 7.89825964060703 1104 78-79 7.73597202582908 1104 80-81 7.642194821849111 1104 82-83 7.388179865601801 1104 84-85 6.980458810166759 1104 86-87 6.848176145191259 1104 88-89 6.415165154709479 1104 90-91 6.139093211961194 1104 92-93 5.609488633936296 1104 94-95 5.478232693576388 1104 96-97 5.027115248124854 1104 98-99 4.672734329132268 1104 100-101 4.055307959610175 1105 1 7.932012312363806 1105 2 9.019060580090809 1105 3 8.568415696709678 1105 4 10.584279677894301 1105 5 10.627888735782978 1105 6 10.306871114073378 1105 7 9.865297980876495 1105 8 9.540779521276736 1105 9 9.441792219703746 1105 10-11 8.94110524422696 1105 12-13 8.523873831671615 1105 14-15 9.145352494371139 1105 16-17 10.3290067569496 1105 18-19 10.851841497795863 1105 20-21 11.259088087345496 1105 22-23 11.378664192605758 1105 24-25 11.25548963030855 1105 26-27 11.47349394501093 1105 28-29 11.09739606803106 1105 30-31 10.84042904183779 1105 32-33 10.7382000599648 1105 34-35 10.971856252430594 1105 36-37 11.179687353752481 1105 38-39 10.898658093521632 1105 40-41 10.642410239373735 1105 42-43 10.450814812084335 1105 44-45 10.210622519155539 1105 46-47 10.141087083443217 1105 48-49 10.019740740298989 1105 50-51 11.543346434407985 1105 52-53 10.347621620465514 1105 54-55 10.207430030980227 1105 56-57 10.21118426032514 1105 58-59 10.157080932158681 1105 60-61 10.033830524835288 1105 62-63 10.078192000243096 1105 64-65 9.702963519986433 1105 66-67 9.633121264789168 1105 68-69 9.498724373912488 1105 70-71 9.417329041326298 1105 72-73 9.12639691264836 1105 74-75 8.732192592591089 1105 76-77 8.560251311117277 1105 78-79 8.185360994629646 1105 80-81 7.7279341373534525 1105 82-83 7.544984660042035 1105 84-85 7.153107322115726 1105 86-87 6.838395150968351 1105 88-89 6.646262372480097 1105 90-91 6.437502752373488 1105 92-93 6.300943284926374 1105 94-95 6.10887493593288 1105 96-97 5.635127251814691 1105 98-99 5.281284466328463 1105 100-101 4.8476130511133935 1106 1 7.900396487584668 1106 2 9.139671262686644 1106 3 8.74639912960032 1106 4 10.34749006278479 1106 5 11.249938649757727 1106 6 10.335386260316294 1106 7 9.57369066196447 1106 8 9.246638033909882 1106 9 8.95708141918566 1106 10-11 8.671865842730696 1106 12-13 8.294927254316548 1106 14-15 8.746999007932196 1106 16-17 9.749789345052985 1106 18-19 11.077554347454662 1106 20-21 11.159914557990597 1106 22-23 11.332146040312072 1106 24-25 11.10029008073288 1106 26-27 11.278298165080912 1106 28-29 11.083813599783074 1106 30-31 10.905173720837961 1106 32-33 10.969435295519322 1106 34-35 10.62390519982954 1106 36-37 11.105069344512014 1106 38-39 10.804058835851851 1106 40-41 10.643427270439485 1106 42-43 10.417261099466653 1106 44-45 10.075113182033224 1106 46-47 9.610669474365139 1106 48-49 9.697474313343125 1106 50-51 11.559889959316644 1106 52-53 11.012489682867649 1106 54-55 10.433491669519537 1106 56-57 10.352640165136755 1106 58-59 10.169837606364641 1106 60-61 9.876909407087023 1106 62-63 9.926646977141935 1106 64-65 9.844619264346463 1106 66-67 9.579963219978715 1106 68-69 9.110357403741016 1106 70-71 8.610878501010419 1106 72-73 8.299906162778846 1106 74-75 8.020812274007701 1106 76-77 7.853865579709364 1106 78-79 7.449992584260224 1106 80-81 7.104370710648896 1106 82-83 7.008723230283097 1106 84-85 6.866384304079421 1106 86-87 6.624623862136167 1106 88-89 6.04017110839226 1106 90-91 5.600263292882678 1106 92-93 5.522512375765169 1106 94-95 5.331764320997349 1106 96-97 4.841182220980922 1106 98-99 4.525085912325872 1106 100-101 3.9715270065349166 1107 1 7.370592642797465 1107 2 8.237755540538146 1107 3 8.35498557929974 1107 4 10.05856655969199 1107 5 10.802633118978402 1107 6 9.752789759928337 1107 7 9.842158040736198 1107 8 8.429123150043921 1107 9 5.801963762315879 1107 10-11 7.9016382156649065 1107 12-13 7.8380093204364485 1107 14-15 8.432998819391138 1107 16-17 9.189779627937082 1107 18-19 10.492931320790607 1107 20-21 10.658853942041468 1107 22-23 10.173891930479659 1107 24-25 10.359013512760203 1107 26-27 10.510737958846 1107 28-29 10.43739319674031 1107 30-31 10.20310441024558 1107 32-33 10.501801123178296 1107 34-35 10.620522628274582 1107 36-37 10.93382156530809 1107 38-39 10.52607390463308 1107 40-41 10.272038810602353 1107 42-43 10.128820841600504 1107 44-45 9.732532635119135 1107 46-47 9.700600221785386 1107 48-49 9.969690468410892 1107 50-51 11.24972672627265 1107 52-53 10.278852943467644 1107 54-55 10.072586237361687 1107 56-57 9.871776066979379 1107 58-59 9.914704141052571 1107 60-61 9.840275445041787 1107 62-63 10.024526905496334 1107 64-65 9.935940284498603 1107 66-67 9.995681742687033 1107 68-69 9.57205630367648 1107 70-71 9.159222747970198 1107 72-73 8.980639152387333 1107 74-75 9.064502457545165 1107 76-77 8.819887290936983 1107 78-79 8.45676294850704 1107 80-81 8.288582572781879 1107 82-83 7.998476894143403 1107 84-85 7.589985873321126 1107 86-87 7.3060561940264375 1107 88-89 7.19978764489308 1107 90-91 7.007342574396228 1107 92-93 6.485639256643482 1107 94-95 6.216317877534289 1107 96-97 5.873658272641171 1107 98-99 5.588576242345233 1107 100-101 5.239776837935704 1108 1 6.462105776795621 1108 2 7.637869026523173 1108 3 7.353474638865208 1108 4 8.796019054460576 1108 5 10.564085233158423 1108 6 9.174411199571718 1108 7 8.263028182441175 1108 8 5.616610111672479 1108 9 6.825669212088979 1108 10-11 5.638749878412028 1108 12-13 4.590130972194654 1108 14-15 6.773246721813912 1108 16-17 8.037268927960092 1108 18-19 7.657462020466422 1108 20-21 7.811960702408886 1108 22-23 8.693582592929157 1108 24-25 8.369286334717938 1108 26-27 6.933048927517749 1108 28-29 8.848566770542696 1108 30-31 9.141168513138005 1108 32-33 9.529694917484182 1108 34-35 9.041298917683793 1108 36-37 8.807844256942058 1108 38-39 8.35720384311259 1108 40-41 8.681195351340055 1108 42-43 7.923066588058116 1108 44-45 7.24663393056856 1108 46-47 8.115119291833345 1108 48-49 8.588944844771735 1108 50-51 10.097622463805424 1108 52-53 9.497350727412083 1108 54-55 9.028744698402722 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-14.17677437920472 2313 70-71 -13.835788165656247 2313 72-73 -13.428465930244407 2313 74-75 -12.985544315139586 2313 76-77 -12.592335970678235 2313 78-79 -12.200705090158381 2313 80-81 -11.771598281599166 2313 82-83 -11.304610103050235 2313 84-85 -10.916406393594997 2313 86-87 -10.450569936313444 2313 88-89 -10.009286255948828 2313 90-91 -9.538806474559182 2313 92-93 -9.01903801183173 2313 94-95 -8.460638779777844 2313 96-97 -7.833856538709 2313 98-99 -7.203441219457073 2313 100-101 -6.321651288038726 2314 1 -18.595107388384324 2314 2 -17.33567757452266 2314 3 -17.961197769624487 2314 4 -19.45375024729273 2314 5 -18.777348171947697 2314 6 -19.75422614278448 2314 7 -19.92491398919832 2314 8 -20.377237935082366 2314 9 -21.628344937403487 2314 10-11 -21.9881341572693 2314 12-13 -21.908638637156315 2314 14-15 -21.836411844780983 2314 16-17 -20.669590499908256 2314 18-19 -19.623220846343788 2314 20-21 -19.290007922629563 2314 22-23 -18.91669116899025 2314 24-25 -18.745601392135338 2314 26-27 -18.468058424066378 2314 28-29 -18.27339570254251 2314 30-31 -18.259477441952736 2314 32-33 -18.130409665720986 2314 34-35 -17.80121107924023 2314 36-37 -17.32872910510039 2314 38-39 -17.223848140892333 2314 40-41 -17.076417690800827 2314 42-43 -16.914521846269782 2314 44-45 -16.55511937610631 2314 46-47 -16.21754757989843 2314 48-49 -15.969037314563852 2314 50-51 -13.818249575567078 2314 52-53 -15.611231247364909 2314 54-55 -16.34852383435643 2314 56-57 -16.30673967982449 2314 58-59 -16.040549990534583 2314 60-61 -15.7350285774091 2314 62-63 -15.317849146889074 2314 64-65 -14.953520270537258 2314 66-67 -14.598331353664697 2314 68-69 -14.17677437920472 2314 70-71 -13.835788165656247 2314 72-73 -13.428465930244407 2314 74-75 -12.985544315139586 2314 76-77 -12.592335970678235 2314 78-79 -12.200705090158381 2314 80-81 -11.771598281599166 2314 82-83 -11.304610103050235 2314 84-85 -10.916406393594997 2314 86-87 -10.450569936313444 2314 88-89 -10.009286255948828 2314 90-91 -9.538806474559182 2314 92-93 -9.01903801183173 2314 94-95 -8.460638779777844 2314 96-97 -7.833856538709 2314 98-99 -7.203441219457073 2314 100-101 -6.321651288038726 2315 1 -18.595107388384324 2315 2 -17.33567757452266 2315 3 -17.961197769624487 2315 4 -19.45375024729273 2315 5 -18.777348171947697 2315 6 -19.75422614278448 2315 7 -19.92491398919832 2315 8 -20.377237935082366 2315 9 -21.628344937403487 2315 10-11 -21.9881341572693 2315 12-13 -21.908638637156315 2315 14-15 -21.836411844780983 2315 16-17 -20.669590499908256 2315 18-19 -19.623220846343788 2315 20-21 -19.290007922629563 2315 22-23 -18.91669116899025 2315 24-25 -18.745601392135338 2315 26-27 -18.468058424066378 2315 28-29 -18.27339570254251 2315 30-31 -18.259477441952736 2315 32-33 -18.130409665720986 2315 34-35 -17.80121107924023 2315 36-37 -17.32872910510039 2315 38-39 -17.223848140892333 2315 40-41 -17.076417690800827 2315 42-43 -16.914521846269782 2315 44-45 -16.55511937610631 2315 46-47 -16.21754757989843 2315 48-49 -15.969037314563852 2315 50-51 -13.818249575567078 2315 52-53 -15.611231247364909 2315 54-55 -16.34852383435643 2315 56-57 -16.30673967982449 2315 58-59 -16.040549990534583 2315 60-61 -15.7350285774091 2315 62-63 -15.317849146889074 2315 64-65 -14.953520270537258 2315 66-67 -14.598331353664697 2315 68-69 -14.17677437920472 2315 70-71 -13.835788165656247 2315 72-73 -13.428465930244407 2315 74-75 -12.985544315139586 2315 76-77 -12.592335970678235 2315 78-79 -12.200705090158381 2315 80-81 -11.771598281599166 2315 82-83 -11.304610103050235 2315 84-85 -10.916406393594997 2315 86-87 -10.450569936313444 2315 88-89 -10.009286255948828 2315 90-91 -9.538806474559182 2315 92-93 -9.01903801183173 2315 94-95 -8.460638779777844 2315 96-97 -7.833856538709 2315 98-99 -7.203441219457073 2315 100-101 -6.321651288038726 2316 1 -18.595107388384324 2316 2 -17.33567757452266 2316 3 -17.961197769624487 2316 4 -19.45375024729273 2316 5 -18.777348171947697 2316 6 -19.75422614278448 2316 7 -19.92491398919832 2316 8 -20.377237935082366 2316 9 -21.628344937403487 2316 10-11 -21.9881341572693 2316 12-13 -21.908638637156315 2316 14-15 -21.836411844780983 2316 16-17 -20.669590499908256 2316 18-19 -19.623220846343788 2316 20-21 -19.290007922629563 2316 22-23 -18.91669116899025 2316 24-25 -18.745601392135338 2316 26-27 -18.468058424066378 2316 28-29 -18.27339570254251 2316 30-31 -18.259477441952736 2316 32-33 -18.130409665720986 2316 34-35 -17.80121107924023 2316 36-37 -17.32872910510039 2316 38-39 -17.223848140892333 2316 40-41 -17.076417690800827 2316 42-43 -16.914521846269782 2316 44-45 -16.55511937610631 2316 46-47 -16.21754757989843 2316 48-49 -15.969037314563852 2316 50-51 -13.818249575567078 2316 52-53 -15.611231247364909 2316 54-55 -16.34852383435643 2316 56-57 -16.30673967982449 2316 58-59 -16.040549990534583 2316 60-61 -15.7350285774091 2316 62-63 -15.317849146889074 2316 64-65 -14.953520270537258 2316 66-67 -14.598331353664697 2316 68-69 -14.17677437920472 2316 70-71 -13.835788165656247 2316 72-73 -13.428465930244407 2316 74-75 -12.985544315139586 2316 76-77 -12.592335970678235 2316 78-79 -12.200705090158381 2316 80-81 -11.771598281599166 2316 82-83 -11.304610103050235 2316 84-85 -10.916406393594997 2316 86-87 -10.450569936313444 2316 88-89 -10.009286255948828 2316 90-91 -9.538806474559182 2316 92-93 -9.01903801183173 2316 94-95 -8.460638779777844 2316 96-97 -7.833856538709 2316 98-99 -7.203441219457073 2316 100-101 -6.321651288038726 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 780276.0 3 32519.0 4 117034.0 5 62668.0 6 37541.0 7 37226.0 8 36066.0 9 35329.0 10 37930.0 11 39666.0 12 40740.0 13 41848.0 14 42941.0 15 43861.0 16 45589.0 17 45969.0 18 45853.0 19 47023.0 20 49211.0 21 52272.0 22 55372.0 23 59098.0 24 63095.0 25 67087.0 26 71394.0 27 76908.0 28 82343.0 29 88241.0 30 95534.0 31 102430.0 32 110100.0 33 117251.0 34 120576.0 35 112430.0 36 83375.0 37 35468.0 38 4452.0 39 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94689233409494 20.865993932109088 14.221576766311287 22.965536967484685 2 14.690374594425961 20.902901237079746 42.08699735596363 22.319726812530664 3 18.579122626974957 26.311943383745106 30.3385482751294 24.770385714150535 4 12.0685930744349 17.581614297033916 40.136957480297255 30.212835148233935 5 12.419479983167674 38.21045703293976 36.1381486600351 13.231914323857467 6 29.10700498389306 39.18770984434891 18.40579947133698 13.299485700421048 7 25.636459184865117 33.28142076026501 23.406019650666437 17.67610040420344 8 23.880209023233633 37.386580390494615 20.946366598670092 17.786843987601664 9 24.921292504207184 17.234641287982495 21.88216728314456 35.96189892466576 10-11 23.605416342607544 27.683145876841802 29.3716742524737 19.339763528076954 12-13 24.16766236986837 25.105247217013638 29.644242352362294 21.0828480607557 14-15 21.502610221612166 26.833226609952774 27.28967007013231 24.37449309830275 16-17 20.888568062019772 29.88958288252904 28.043052652363965 21.178796403087222 18-19 20.627640754617005 28.769676861347097 30.116539689661497 20.486142694374397 20-21 21.386234891901864 28.06644120303188 30.141199927454522 20.40612397761174 22-23 21.041739555592496 27.950530174796445 29.914752749542465 21.09297752006859 24-25 20.933526072681108 28.12500497424874 30.120298368514632 20.82117058455552 26-27 20.870740459269033 28.297487447444436 29.87788922201529 20.953882871271233 28-29 20.75221431980227 28.439919547270875 30.01674317807868 20.791122954848177 30-31 21.12417443375798 28.266876625700142 29.855247464929636 20.753701475612242 32-33 20.91655868707627 28.4456523746277 29.72161307771333 20.916175860582698 34-35 21.02305595304089 28.45031467456865 29.84804933356645 20.678580038824013 36-37 21.125301760660687 28.25523527624947 29.73769376060587 20.881769202483973 38-39 21.00834337378342 28.477957761012423 29.744267105106008 20.769431760098144 40-41 21.223335104932655 28.41156090497014 29.694934112104665 20.67016987799254 42-43 21.038472848689853 28.468872242687304 29.483499744720536 21.009155163902307 44-45 21.028108336503042 28.658281742285595 29.446452108207165 20.8671578130042 46-47 21.152926570653804 28.54722076827806 29.13205283182503 21.167799829243112 48-49 21.13623961290076 28.629382887167427 29.112548232241505 21.12182926769031 50-51 21.39901036096396 28.874360863912358 28.68107416086104 21.04555461426264 52-53 20.851953660011198 29.080776215000885 28.805320407042167 21.26194971794575 54-55 20.83338544696895 28.927855032948035 28.974369148267677 21.264390371815338 56-57 20.85270038718583 29.041376853925506 28.95095660937168 21.154966149516984 58-59 20.877766573086017 29.032481210668898 29.00204407543139 21.087708140813692 60-61 20.907474242340847 29.060855609460333 28.98864767156702 21.043022476631798 62-63 20.83166905727128 28.95119464167719 28.908747434982164 21.30838886606937 64-65 20.651630668547288 28.876330044382353 29.07523974826566 21.3967995388047 66-67 20.686457138961103 28.878745664233886 28.849494634694434 21.58530256211058 68-69 20.67876162501852 28.928319285981992 28.884956986341965 21.507962102657523 70-71 20.779644997921327 28.725319245935694 28.968906234177634 21.52612952196534 72-73 20.791685356399608 28.826212251805146 28.894188490195916 21.487913901599327 74-75 20.630845319294128 29.069018035807886 28.68026412519244 21.619872519705545 76-77 20.72449050348585 28.95531876678804 28.66531390439297 21.65487682533314 78-79 20.61187925475341 29.066990662609165 28.624781495660113 21.696348586977308 80-81 20.705784182577585 28.97941772603123 28.512165033778363 21.80263305761282 82-83 20.809636328485812 28.856808368955818 28.674650398470558 21.658904904087812 84-85 20.88821961787904 28.906948735879595 28.459530457754916 21.745301188486444 86-87 20.657830436168855 28.93087539541032 28.60103928299209 21.810254885428733 88-89 20.819575927176494 28.84964411407288 28.546067479194647 21.784712479555974 90-91 20.76043362728045 28.832406337396193 28.62710459037268 21.780055444950676 92-93 20.72535019367577 28.828565881139472 28.46762179967477 21.97846212550999 94-95 20.54899834465333 28.852467748204212 28.4368833542367 22.161650552905762 96-97 20.50371246225441 28.716680728246118 28.525627998349563 22.253978811149906 98-99 20.427776803137377 28.731427751740956 28.250682129421513 22.59011331570015 100-101 20.685496385183285 28.82349343808217 28.150639966917918 22.340370209816626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 179835.0 1 163160.5 2 108567.5 3 55392.0 4 34495.5 5 27180.0 6 25276.0 7 25462.5 8 26208.5 9 25967.5 10 23784.0 11 20045.0 12 15955.5 13 12108.0 14 9183.0 15 7585.0 16 7019.0 17 7446.5 18 8647.5 19 10164.5 20 11745.5 21 13607.0 22 15562.0 23 17615.0 24 19887.5 25 22674.0 26 25988.5 27 29838.5 28 34823.5 29 40696.0 30 46375.5 31 52429.0 32 59340.5 33 65455.5 34 70787.0 35 75834.5 36 80819.5 37 85245.5 38 87505.0 39 87707.5 40 87505.5 41 87320.5 42 87065.5 43 87320.5 44 87151.0 45 86982.5 46 86014.5 47 83800.5 48 81514.5 49 78242.5 50 73884.5 51 68629.5 52 62921.0 53 56769.0 54 50604.0 55 44208.0 56 36877.0 57 30084.5 58 24212.0 59 19203.0 60 14852.0 61 10970.0 62 7906.5 63 5437.0 64 3614.0 65 2352.5 66 1476.5 67 887.0 68 550.5 69 330.0 70 183.5 71 118.0 72 86.0 73 63.0 74 38.5 75 22.0 76 18.5 77 13.5 78 5.0 79 3.5 80 3.0 81 2.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.120957984586063 2 29.12839976796221 3 22.930769868235686 4 29.258539819342005 5 32.41289467141792 6 24.99580674567001 7 23.92859865749565 8 19.92017900058009 9 11.735907847849507 10-11 15.375702328664953 12-13 15.453965360072926 14-15 19.606430761581173 16-17 22.945255655921105 18-19 26.932278113864257 20-21 28.992972569818516 22-23 28.609380956327175 24-25 27.11280351371509 26-27 28.554305129692548 28-29 25.53947128532361 30-31 27.417469130687 32-33 26.400033148255574 34-35 24.446523576696777 36-37 27.430678710532856 38-39 27.56022209331234 40-41 24.563802104914227 42-43 25.43314825557305 44-45 24.13718405568907 46-47 27.455324438551422 48-49 22.82461257976299 50-51 28.4339438137068 52-53 22.0078727106986 54-55 23.14076406729096 56-57 25.268152813458194 58-59 24.52725615314494 60-61 24.11628408055026 62-63 23.898466893179744 64-65 22.43286649540068 66-67 22.940101102179497 68-69 23.937349796966934 70-71 22.418596171376482 72-73 22.293809563271733 74-75 19.558200049722384 76-77 21.84000994447667 78-79 20.96156459766305 80-81 21.980558548106405 82-83 22.842462915389078 84-85 19.796204524736886 86-87 21.34934946548438 88-89 20.185829120742522 90-91 21.30383691058258 92-93 21.295135493494655 94-95 20.340697770779812 96-97 19.26029667688738 98-99 18.65761166818596 100-101 20.320941410458275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3016750.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.13823276216833 #Duplication Level Percentage of deduplicated Percentage of total 1 88.46744253687 67.35754731745959 2 7.587980098159351 11.554707898167154 3 1.966118909546553 4.490904575194681 4 0.7381377676968616 2.2480202066980395 5 0.35968593421311557 1.3692925690198081 6 0.213211928136671 0.9740147647284338 7 0.1448583045234978 0.7720478715140197 8 0.0964824445272904 0.5876802255105466 9 0.07062128285383681 0.4839281704699519 >10 0.3387479041131797 4.398570860222565 >50 0.012217344122711206 0.645679456824203 >100 0.004276991514251315 0.5436884213539979 >500 8.742148918112023E-5 0.0481570114637053 >1k 8.742148895100803E-5 0.12989413641137246 >5k 0.0 0.0 >10k+ 4.371074447550402E-5 4.395866514961858 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 132085 4.378387337366371 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.314825557305047E-5 0.0 4 0.0 0.0 0.0 3.314825557305047E-5 0.0 5 0.0 0.0 0.0 6.629651114610094E-5 0.0 6 9.94447667191514E-5 0.0 0.0 6.629651114610094E-5 3.314825557305047E-5 7 9.94447667191514E-5 0.0 0.0 6.629651114610094E-5 3.314825557305047E-5 8 9.94447667191514E-5 0.0 0.0 6.629651114610094E-5 3.314825557305047E-5 9 9.94447667191514E-5 0.0 0.0 1.3259302229220188E-4 3.314825557305047E-5 10-11 9.94447667191514E-5 0.0 0.0 1.3259302229220188E-4 3.314825557305047E-5 12-13 9.94447667191514E-5 0.0 0.0 1.3259302229220188E-4 3.314825557305047E-5 14-15 9.94447667191514E-5 0.0 0.0 1.6574127786525233E-4 3.314825557305047E-5 16-17 9.94447667191514E-5 0.0 0.0 1.6574127786525233E-4 6.629651114610094E-5 18-19 1.1601889450567664E-4 0.0 0.0 1.6574127786525233E-4 6.629651114610094E-5 20-21 1.3259302229220188E-4 0.0 0.0 1.8231540565177758E-4 6.629651114610094E-5 22-23 1.3259302229220188E-4 0.0 0.0 2.1546366122482803E-4 6.629651114610094E-5 24-25 1.3259302229220188E-4 0.0 0.0 2.81760172370929E-4 6.629651114610094E-5 26-27 1.3259302229220188E-4 0.0 0.0 3.6463081130355516E-4 6.629651114610094E-5 28-29 1.3259302229220188E-4 0.0 0.0 5.63520344741858E-4 6.629651114610094E-5 30-31 1.3259302229220188E-4 0.0 0.0 0.0013756526062815944 6.629651114610094E-5 32-33 1.491671500787271E-4 0.0 0.0 0.0037291787519681775 9.94447667191514E-5 34-35 1.6574127786525233E-4 0.0 0.0 0.007624098781801607 9.94447667191514E-5 36-37 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.01349134001823154 9.94447667191514E-5 38-39 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.023154056517775752 9.94447667191514E-5 40-41 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.0334300157454214 9.94447667191514E-5 42-43 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.04395458688986492 9.94447667191514E-5 44-45 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.05708129609679291 9.94447667191514E-5 46-47 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.0744178337614983 9.94447667191514E-5 48-49 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.09500290047236265 9.94447667191514E-5 50-51 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.1170464904284412 9.94447667191514E-5 52-53 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.1416259219358581 9.94447667191514E-5 54-55 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.170779812712356 9.94447667191514E-5 56-57 1.6574127786525233E-4 0.0 3.314825557305047E-5 0.20656335460346398 9.94447667191514E-5 58-59 1.988895334383028E-4 0.0 3.314825557305047E-5 0.24746830198060826 9.94447667191514E-5 60-61 1.988895334383028E-4 0.0 3.314825557305047E-5 0.2922847435153725 9.94447667191514E-5 62-63 1.988895334383028E-4 1.6574127786525235E-5 3.314825557305047E-5 0.3392392475345985 1.3259302229220188E-4 64-65 1.988895334383028E-4 3.314825557305047E-5 3.314825557305047E-5 0.3890445015331068 1.3259302229220188E-4 66-67 1.988895334383028E-4 3.314825557305047E-5 3.314825557305047E-5 0.4481478412198558 1.3259302229220188E-4 68-69 1.988895334383028E-4 3.314825557305047E-5 3.314825557305047E-5 0.5151404657329908 1.3259302229220188E-4 70-71 1.988895334383028E-4 3.314825557305047E-5 3.314825557305047E-5 0.5901052457114444 1.988895334383028E-4 72-73 2.3203778901135328E-4 3.314825557305047E-5 3.314825557305047E-5 0.6686500372917875 1.988895334383028E-4 74-75 2.3203778901135328E-4 3.314825557305047E-5 3.314825557305047E-5 0.7501781718737051 1.988895334383028E-4 76-77 2.3203778901135328E-4 3.314825557305047E-5 3.314825557305047E-5 0.8417170796386839 1.988895334383028E-4 78-79 2.3203778901135328E-4 3.314825557305047E-5 3.314825557305047E-5 0.9492334465898733 1.988895334383028E-4 80-81 2.3203778901135328E-4 3.314825557305047E-5 3.314825557305047E-5 1.0704566172205188 2.1546366122482803E-4 82-83 2.3203778901135328E-4 3.314825557305047E-5 3.314825557305047E-5 1.19570730090329 2.983343001574542E-4 84-85 2.3203778901135328E-4 3.314825557305047E-5 3.314825557305047E-5 1.320891688074915 2.983343001574542E-4 86-87 2.3203778901135328E-4 3.314825557305047E-5 3.314825557305047E-5 1.4546946216955332 2.983343001574542E-4 88-89 2.3203778901135328E-4 6.629651114610094E-5 3.314825557305047E-5 1.606563354603464 2.983343001574542E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2505 0.0 55.412853 1 GTATCAA 4755 0.0 43.41137 1 ATCAACG 5425 0.0 36.30662 3 TCAACGC 5580 0.0 35.29284 4 TATCAAC 5700 0.0 34.41351 2 CAACGCA 5700 0.0 34.370544 5 AACGCAG 6200 0.0 31.401266 6 GTGGTAT 1365 0.0 29.269205 1 ACGCAGA 6905 0.0 27.324017 7 CGCAGAG 7000 0.0 27.05509 8 TGGTATC 1380 0.0 25.236834 2 GTACATG 4825 0.0 24.416279 1 GCAGAGT 7780 0.0 23.042603 9 TACATGG 4930 0.0 22.751091 2 ACATGGG 4915 0.0 21.318106 3 GTGTAGC 1075 0.0 20.964918 1 GAGTACT 4955 0.0 20.938751 12-13 CAGAGTA 7420 0.0 19.817005 10-11 AGAGTAC 6700 0.0 19.17763 10-11 GTACTTT 5610 0.0 18.418106 14-15 >>END_MODULE