FastQCFastQC Report
Sat 18 Jun 2016
SRR1931037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016750
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75710.2509654429435651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65960.21864589375984086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.12298002817601723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.055.585641
GTATCAA79800.041.394961
ATCAACG96900.033.5961153
CAACGCA97850.033.166315
TCAACGC98300.033.115994
AACGCAG102500.031.9851846
TATCAAC102350.031.9485952
ACGCAGA116800.027.70367
CGCAGAG117550.027.4057588
TGGTATC20800.025.3635942
GTGGTAT23400.024.5773531
GCAGAGT134550.023.2378759
GAGTACT94600.021.03950712-13
GGCGTCG3001.6916601E-1020.5597028
AGAGTAC124350.020.41280410-11
CAGAGTA131750.020.20258510-11
TATACCG3302.7284841E-1120.1287145
GTACATG77500.019.3185441
ACGGGCG3254.965841E-1018.97855
TACATGG77200.018.9620192
Produced by FastQC (version 0.11.5)