##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931037_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3016750 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.342665782713183 31.0 31.0 34.0 30.0 34.0 2 31.501627579348636 31.0 31.0 34.0 30.0 34.0 3 31.56819027098699 31.0 31.0 34.0 30.0 34.0 4 35.33983889947792 37.0 35.0 37.0 33.0 37.0 5 35.21751487527969 37.0 35.0 37.0 33.0 37.0 6 35.24426319714925 37.0 35.0 37.0 32.0 37.0 7 35.12455887958896 37.0 35.0 37.0 32.0 37.0 8 35.13092367614154 37.0 35.0 37.0 32.0 37.0 9 36.63251313499627 39.0 35.0 39.0 32.0 39.0 10-11 36.579853318969086 38.5 35.0 39.0 32.0 39.0 12-13 36.45850534515621 38.0 35.0 39.0 32.0 39.0 14-15 37.51894290212978 39.0 36.0 41.0 32.0 41.0 16-17 37.47955183558465 39.0 36.0 41.0 32.0 41.0 18-19 37.506696610590865 39.0 36.0 41.0 32.0 41.0 20-21 37.49250882572305 39.0 36.0 41.0 32.0 41.0 22-23 37.39215761995525 39.0 36.0 41.0 32.0 41.0 24-25 37.27159376812796 39.0 36.0 40.5 31.0 41.0 26-27 37.01748570481479 39.0 36.0 40.0 31.0 41.0 28-29 36.836307450070436 39.0 36.0 40.0 30.5 41.0 30-31 36.60782149664374 38.5 35.0 40.0 30.0 41.0 32-33 36.44306289881495 38.0 35.0 40.0 30.0 41.0 34-35 36.523843374492415 38.0 35.0 40.0 30.0 41.0 36-37 36.587500455788515 38.5 35.0 40.0 30.0 41.0 38-39 36.57677252009613 39.0 35.0 40.0 30.0 41.0 40-41 36.46316731582 39.0 35.0 40.0 30.0 41.0 42-43 36.3260202204359 39.0 35.0 40.0 30.0 41.0 44-45 36.16504499875694 38.0 35.0 40.0 30.0 41.0 46-47 35.92980144194912 38.0 35.0 40.0 29.0 41.0 48-49 35.709693875859784 38.0 35.0 40.0 28.0 41.0 50-51 35.45384602635286 38.0 34.5 40.0 27.5 41.0 52-53 35.24976464738543 38.0 34.0 40.0 27.0 41.0 54-55 35.103913483052956 38.0 34.0 40.0 26.5 41.0 56-57 34.93445363387752 38.0 34.0 40.0 26.0 41.0 58-59 34.73328764398774 38.0 33.5 40.0 26.0 41.0 60-61 34.434102428109725 37.5 33.0 40.0 26.0 41.0 62-63 34.189642661804925 37.0 33.0 40.0 25.0 41.0 64-65 33.88657512223419 37.0 32.5 40.0 24.5 41.0 66-67 33.573165824148504 36.0 32.0 39.0 23.5 41.0 68-69 33.23475462003812 36.0 32.0 39.0 23.0 40.0 70-71 32.8686507002569 35.5 31.0 39.0 22.5 40.0 72-73 32.447594928316896 35.0 31.0 38.0 22.0 40.0 74-75 31.93685936852573 35.0 31.0 37.5 21.0 39.5 76-77 30.540043092732247 33.5 29.5 36.0 20.0 39.0 78-79 31.278178503356262 34.0 30.0 36.0 20.5 39.0 80-81 31.242787271069858 34.0 31.0 36.0 20.5 38.5 82-83 30.962954504019226 34.0 30.5 36.0 20.0 37.0 84-85 30.62469163835253 34.0 30.0 35.0 19.5 37.0 86-87 30.272769039529294 34.0 30.0 35.0 18.0 36.5 88-89 29.913155713930557 34.0 30.0 35.0 16.0 36.0 90-91 29.572436562525894 34.0 29.0 35.0 9.5 36.0 92-93 29.252742852407394 34.0 29.0 35.0 6.0 35.5 94-95 28.873170464904284 34.0 29.0 35.0 2.0 35.0 96-97 28.445017651446094 34.0 29.0 35.0 2.0 35.0 98-99 27.953417916632137 34.0 29.0 35.0 2.0 35.0 100-101 26.825277533769786 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3321960673372395 1101 2 0.431694987631456 1101 3 0.27688705752252574 1101 4 0.15725471601805907 1101 5 0.27474226160585147 1101 6 0.3379408268447506 1101 7 0.37260334041241805 1101 8 0.3314241123870403 1101 9 0.41463623933759663 1101 10-11 0.4126778051653872 1101 12-13 0.4238757252097116 1101 14-15 0.4689132017132849 1101 16-17 0.5441459299994094 1101 18-19 0.5015784844283502 1101 20-21 0.4610698117389589 1101 22-23 0.5515465289928798 1101 24-25 0.3818120768198341 1101 26-27 0.42041119091135926 1101 28-29 0.45088577369263305 1101 30-31 0.54470550114128 1101 32-33 0.45273732817880585 1101 34-35 0.4249526749046666 1101 36-37 0.43005588795624305 1101 38-39 0.549205000233222 1101 40-41 0.6195505309112832 1101 42-43 0.5218850694627335 1101 44-45 0.5455258789282027 1101 46-47 0.46282923908374585 1101 48-49 0.4287492816005525 1101 50-51 0.40024619931703853 1101 52-53 0.3696122751310682 1101 54-55 0.3134124244614185 1101 56-57 0.4542162850499949 1101 58-59 0.4183176577403174 1101 60-61 0.2697992669119813 1101 62-63 0.1974839123626353 1101 64-65 0.3023131977721647 1101 66-67 0.20673494919939372 1101 68-69 0.19054975694637477 1101 70-71 0.29209850294454043 1101 72-73 0.20412348190229324 1101 74-75 0.31722677161876334 1101 76-77 0.1966053005394457 1101 78-79 0.14893087941176475 1101 80-81 0.13317360595596384 1101 82-83 0.2550081881774027 1101 84-85 0.2668333550479325 1101 86-87 0.25423672108377104 1101 88-89 0.273511533165415 1101 90-91 0.21984847771547322 1101 92-93 0.17016504610811012 1101 94-95 0.27884656668383556 1101 96-97 0.4237923215097368 1101 98-99 0.5139298208559602 1101 100-101 0.8411829384495242 1102 1 0.35650926372421665 1102 2 0.4238862991977612 1102 3 0.4126770962304853 1102 4 0.24623756322200308 1102 5 0.3448568250984536 1102 6 0.2541297208540101 1102 7 0.41504145292868344 1102 8 0.43062581924056786 1102 9 0.5196386267271862 1102 10-11 0.4580651500240762 1102 12-13 0.48913905064055996 1102 14-15 0.6050212469561345 1102 16-17 0.6377200030524861 1102 18-19 0.5796753032187212 1102 20-21 0.44947245565590777 1102 22-23 0.5842078390300074 1102 24-25 0.5129491504315311 1102 26-27 0.5507192317851377 1102 28-29 0.5642840225241699 1102 30-31 0.44232794197927205 1102 32-33 0.6379637036845054 1102 34-35 0.6271078791539111 1102 36-37 0.6927482704670282 1102 38-39 0.7435078772717318 1102 40-41 0.7192965521886947 1102 42-43 0.6587632689028666 1102 44-45 0.7128488089990128 1102 46-47 0.6271348519979156 1102 48-49 0.6291160241712959 1102 50-51 0.484330996898656 1102 52-53 0.4235486072476533 1102 54-55 0.6411790657111425 1102 56-57 0.4331007343535944 1102 58-59 0.5871158281364757 1102 60-61 0.40886491856531393 1102 62-63 0.3265320981025823 1102 64-65 0.23804414988809697 1102 66-67 0.14568039916771625 1102 68-69 0.30822236937474656 1102 70-71 0.29878543034813276 1102 72-73 0.37506406138779624 1102 74-75 0.4132563710886501 1102 76-77 0.45396501409430456 1102 78-79 0.5293268740065926 1102 80-81 0.4351630452678954 1102 82-83 0.38606755459853304 1102 84-85 0.4378083670368902 1102 86-87 0.4004256358981735 1102 88-89 0.501507655087682 1102 90-91 0.6377002223823354 1102 92-93 0.5559643431024917 1102 94-95 0.6197781791548529 1102 96-97 0.7139399963795228 1102 98-99 0.7815645679330956 1102 100-101 0.8733215193934747 1103 1 0.3656181612575544 1103 2 0.40053300823266014 1103 3 0.4054862163707931 1103 4 0.2730160171562588 1103 5 0.29957071687752546 1103 6 0.3748650802038114 1103 7 0.4177072174477061 1103 8 0.39799907737675966 1103 9 0.5655322537082128 1103 10-11 0.4747868381576339 1103 12-13 0.4456793825682084 1103 14-15 0.5311523213771849 1103 16-17 0.5314455116859094 1103 18-19 0.5362584599041895 1103 20-21 0.5529788732371586 1103 22-23 0.5868497893042317 1103 24-25 0.6320462328288201 1103 26-27 0.7149404242144541 1103 28-29 0.6055311121781557 1103 30-31 0.49974980979283146 1103 32-33 0.5666576201159259 1103 34-35 0.6671423842571045 1103 36-37 0.6951131374036592 1103 38-39 0.6115148191751913 1103 40-41 0.6838269209722085 1103 42-43 0.6314480234934976 1103 44-45 0.6844171558815901 1103 46-47 0.6899928066711283 1103 48-49 0.5619716076407357 1103 50-51 0.5592234239688025 1103 52-53 0.6157696279691081 1103 54-55 0.6382105638826516 1103 56-57 0.5652124548459057 1103 58-59 0.642285052364457 1103 60-61 0.49859740180846046 1103 62-63 0.44532899836689666 1103 64-65 0.4155276281169762 1103 66-67 0.3651335709593866 1103 68-69 0.2961207469398843 1103 70-71 0.3703314960918007 1103 72-73 0.3139686674511353 1103 74-75 0.31098430816180667 1103 76-77 0.4935049392378552 1103 78-79 0.508556048807236 1103 80-81 0.493385296562348 1103 82-83 0.5180858578387024 1103 84-85 0.4859717061824007 1103 86-87 0.33526884004859525 1103 88-89 0.33088993606306616 1103 90-91 0.35383718392999697 1103 92-93 0.24224437409167265 1103 94-95 0.4158685547249128 1103 96-97 0.5368642221099478 1103 98-99 0.5421682437077742 1103 100-101 0.7114995354726865 1104 1 0.2705856076043105 1104 2 0.3523645622585718 1104 3 0.3556429561827059 1104 4 0.16344063955580168 1104 5 0.2870929125501931 1104 6 0.27508648888917264 1104 7 0.31586799084359285 1104 8 0.3153741787644009 1104 9 0.4433804243463513 1104 10-11 0.3461922352683544 1104 12-13 0.38515150918766494 1104 14-15 0.45046420072041116 1104 16-17 0.4694061403988954 1104 18-19 0.442169633370213 1104 20-21 0.39585433633205724 1104 22-23 0.46430994670168957 1104 24-25 0.47138134912318463 1104 26-27 0.5107435239360427 1104 28-29 0.5639972541866172 1104 30-31 0.49925876478768316 1104 32-33 0.4829278702419728 1104 34-35 0.4827495044096821 1104 36-37 0.43897945916966563 1104 38-39 0.40846770954501466 1104 40-41 0.4883230518006201 1104 42-43 0.5225850995835515 1104 44-45 0.5808592717594507 1104 46-47 0.4683407909752262 1104 48-49 0.46097855680442024 1104 50-51 0.34969956225058496 1104 52-53 0.21391944053898015 1104 54-55 0.37384092098636756 1104 56-57 0.5980247835829999 1104 58-59 0.4645190879213743 1104 60-61 0.374293962301266 1104 62-63 0.22218521425131854 1104 64-65 0.3071754786989942 1104 66-67 0.3712113489807365 1104 68-69 0.2050724535405024 1104 70-71 0.3687498488988652 1104 72-73 0.3427539887129427 1104 74-75 0.5825716549842639 1104 76-77 0.4446251356558584 1104 78-79 0.6749764622495924 1104 80-81 0.5933951613057538 1104 82-83 0.5470947799510704 1104 84-85 0.5490392029489577 1104 86-87 0.5323773741477282 1104 88-89 0.5724840785976504 1104 90-91 0.6739711252238152 1104 92-93 0.7650461804357995 1104 94-95 0.9277895490910488 1104 96-97 0.8164266659357153 1104 98-99 0.9738384543748495 1104 100-101 1.205423949616538 1105 1 0.26041569539424003 1105 2 0.2993884601939847 1105 3 0.3359158241694793 1105 4 0.24015910983778355 1105 5 0.2647440042566629 1105 6 0.2467322178270166 1105 7 0.22468294405616973 1105 8 0.3089068048793635 1105 9 0.4137866970658095 1105 10-11 0.40162145219469636 1105 12-13 0.39793508707680303 1105 14-15 0.40840877893005967 1105 16-17 0.31630759757101856 1105 18-19 0.4108200097802879 1105 20-21 0.3654711446247987 1105 22-23 0.3720605545081881 1105 24-25 0.5034380607922841 1105 26-27 0.3845089536220172 1105 28-29 0.3694482622464079 1105 30-31 0.2975910101929742 1105 32-33 0.46340672795662385 1105 34-35 0.45175596552961395 1105 36-37 0.4406277678650099 1105 38-39 0.5229956530092394 1105 40-41 0.4437824636159178 1105 42-43 0.3893196151754452 1105 44-45 0.44834768239429934 1105 46-47 0.3920421175959774 1105 48-49 0.3329106974559295 1105 50-51 0.16772610639180385 1105 52-53 0.20196089646030657 1105 54-55 0.2158429887010911 1105 56-57 0.2829041799197398 1105 58-59 0.3656219342538165 1105 60-61 0.31359305000520266 1105 62-63 0.2069875110172461 1105 64-65 0.28697197101053007 1105 66-67 0.23853999054297503 1105 68-69 0.21101723109504178 1105 70-71 0.12795577217844567 1105 72-73 0.15501350450028184 1105 74-75 0.24515842864572335 1105 76-77 0.2872418501392424 1105 78-79 0.3839714121788447 1105 80-81 0.28408575457180874 1105 82-83 0.320946494708064 1105 84-85 0.3300581485317089 1105 86-87 0.3885598020904091 1105 88-89 0.3129269565125803 1105 90-91 0.2952555903931753 1105 92-93 0.29466121025935976 1105 94-95 0.19655699176961505 1105 96-97 0.34763459829916243 1105 98-99 0.3485169618678725 1105 100-101 0.43375193451323213 1106 1 0.23255153795837202 1106 2 0.2537399844840351 1106 3 0.24721983159278338 1106 4 0.12273348784624716 1106 5 0.1584394168878802 1106 6 0.19588549741872896 1106 7 0.26017019492125826 1106 8 0.21271859323792341 1106 9 0.31878466725660815 1106 10-11 0.30945825553517636 1106 12-13 0.31067847086539047 1106 14-15 0.40321212908181536 1106 16-17 0.32154387702741616 1106 18-19 0.3444274156841374 1106 20-21 0.33350421118321094 1106 22-23 0.35297899484147166 1106 24-25 0.30018113527661683 1106 26-27 0.4672014471942916 1106 28-29 0.4676068870023471 1106 30-31 0.4261363619299203 1106 32-33 0.41150093043491864 1106 34-35 0.5648002443674045 1106 36-37 0.4114010285118468 1106 38-39 0.46374242947891986 1106 40-41 0.42914101491209067 1106 42-43 0.4498021781478556 1106 44-45 0.38861291079616933 1106 46-47 0.4504542268577083 1106 48-49 0.4094891640800711 1106 50-51 0.29395486588840214 1106 52-53 0.1925894607086036 1106 54-55 0.4058476765937655 1106 56-57 0.42753828264741855 1106 58-59 0.4222336027569824 1106 60-61 0.35841974296273094 1106 62-63 0.2598183215877228 1106 64-65 0.3169523978504145 1106 66-67 0.3371572555200686 1106 68-69 0.19221249873988455 1106 70-71 0.37844220145409224 1106 72-73 0.39165146386542915 1106 74-75 0.5492769598371297 1106 76-77 0.4208009272526603 1106 78-79 0.4508929648862221 1106 80-81 0.4519264242155252 1106 82-83 0.4176386306472857 1106 84-85 0.59730461569713 1106 86-87 0.5415360823200643 1106 88-89 0.6193475813071814 1106 90-91 0.5578009227985987 1106 92-93 0.5103119090936694 1106 94-95 0.7416346561358331 1106 96-97 0.8027664875682383 1106 98-99 0.8378074952244035 1106 100-101 0.9987256919800309 1107 1 0.27681111548137594 1107 2 0.3139745005499073 1107 3 0.23048550769375353 1107 4 0.17766158844015223 1107 5 0.27205947765610716 1107 6 0.18014957092074013 1107 7 0.29556747267849204 1107 8 0.30997445070088503 1107 9 0.27378745911453706 1107 10-11 0.2707996526243974 1107 12-13 0.2839088317764791 1107 14-15 0.38358732578088706 1107 16-17 0.3552135385962174 1107 18-19 0.4531926893224636 1107 20-21 0.42201277892383615 1107 22-23 0.3768118894542667 1107 24-25 0.3274902918454572 1107 26-27 0.37760891342006175 1107 28-29 0.4011516763802305 1107 30-31 0.5385884833793568 1107 32-33 0.5484944257685314 1107 34-35 0.4957027033778445 1107 36-37 0.4972182452107745 1107 38-39 0.6223296768520328 1107 40-41 0.5924777564875683 1107 42-43 0.5304712088292902 1107 44-45 0.543161318711995 1107 46-47 0.36780508000597933 1107 48-49 0.4610684854773055 1107 50-51 0.42313086896307794 1107 52-53 0.4286290833364319 1107 54-55 0.47832459844349273 1107 56-57 0.5537740604880526 1107 58-59 0.4914574662532587 1107 60-61 0.561551264851623 1107 62-63 0.2934733349668832 1107 64-65 0.401451567544612 1107 66-67 0.40045527366351763 1107 68-69 0.35145717426798484 1107 70-71 0.3455896478105345 1107 72-73 0.39123101731319565 1107 74-75 0.46113300149019665 1107 76-77 0.6220658926988847 1107 78-79 0.5127921534345319 1107 80-81 0.5463193582769215 1107 82-83 0.5838854308880421 1107 84-85 0.5682618966450583 1107 86-87 0.42944741001166875 1107 88-89 0.5605707776581887 1107 90-91 0.6495777772086448 1107 92-93 0.5673464483645958 1107 94-95 0.6616356713568976 1107 96-97 0.8266639212344202 1107 98-99 0.899885072616879 1107 100-101 1.086441082006207 1108 1 0.02081408806221674 1108 2 0.07686322146061286 1108 3 -0.018145673117754058 1108 4 0.05236172388095639 1108 5 0.025183531400045922 1108 6 0.03566288255246519 1108 7 0.04226295588448892 1108 8 0.06337329936293656 1108 9 0.033914323247969946 1108 10-11 -0.02256895612777754 1108 12-13 0.09859169494194475 1108 14-15 0.023112934796934326 1108 16-17 0.13877201672920592 1108 18-19 0.07331312526902423 1108 20-21 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-0.9106102663690159 2316 88-89 -0.903312765308133 2316 90-91 -0.8004985883849329 2316 92-93 -0.8544557796437999 2316 94-95 -1.2761612259557076 2316 96-97 -1.061824437233028 2316 98-99 -1.0637488174206382 2316 100-101 -1.448836644463956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 14.0 4 32.0 5 67.0 6 142.0 7 406.0 8 943.0 9 1847.0 10 3311.0 11 5299.0 12 7533.0 13 9948.0 14 12477.0 15 15689.0 16 18663.0 17 22015.0 18 19104.0 19 15088.0 20 14412.0 21 15011.0 22 16820.0 23 19822.0 24 24429.0 25 30815.0 26 39897.0 27 51843.0 28 66224.0 29 83061.0 30 101482.0 31 123483.0 32 149026.0 33 180006.0 34 223384.0 35 279085.0 36 365658.0 37 504689.0 38 496949.0 39 98044.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.856156318620116 22.024406220496665 15.147609500472054 22.971827960411165 2 14.746248172569143 21.976642035716534 41.2214697019423 22.055640089772023 3 18.34747580288542 26.639765145945116 31.186117728020392 23.826641323149076 4 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20.718838283347022 24-25 20.507384159261292 28.313308326106963 30.688845663248394 20.490461851383355 26-27 20.47488747042872 28.444750744326946 30.526065075796787 20.554296709447545 28-29 20.41627648342833 28.518585428997145 30.599454047325843 20.46568404024868 30-31 20.82310151450533 28.26317461838182 30.48746912635862 20.426254740754235 32-33 20.65276206567739 28.516622260181773 30.27049053140571 20.56012514273513 34-35 20.721001773346494 28.45170175289118 30.48572918555689 20.34156728820544 36-37 20.77694815898162 28.291566302202714 30.54118331091103 20.390302227904638 38-39 20.59386222262516 28.5839681099835 30.460262862918242 20.3619068044731 40-41 20.789533134339834 28.49545104446724 30.35408278931667 20.360933031876254 42-43 20.63265391709324 28.539278014088705 30.172754603725615 20.655313465092444 44-45 20.652705048353926 28.750760813496928 30.064730476431688 20.531803661717458 46-47 20.746375831450305 28.640164353988308 29.824815277468357 20.788644537093028 48-49 20.819836368886417 28.726082152380023 29.72828045440637 20.72580102432719 50-51 20.84334096304395 28.934858301262423 29.432129008088907 20.78967172760472 52-53 20.851661598635133 29.055069585737787 29.26686164456434 20.82640717106274 54-55 20.835604402891057 28.89841522445461 29.421556925933295 20.844423446721038 56-57 20.879396830832476 29.03314350727416 29.278491354397357 20.808968307496002 58-59 20.856611202055575 29.007961209308107 29.450208251310638 20.68521933732568 60-61 20.86923765595739 29.049590239765948 29.417855871598224 20.66331623267843 62-63 20.828287644548706 28.92308152376617 29.429497280769013 20.819133550916117 64-65 20.616994428342267 28.843010838440204 29.494779322645254 21.045215410572276 66-67 20.655625585828034 28.924023847033105 29.352009598897745 21.06834096824112 68-69 20.7110798889027 28.937653651037763 29.36013377401841 20.991132686041126 70-71 20.74105183834224 28.728496908864454 29.438246150845554 21.092205101947748 72-73 20.86093105509606 28.8706297601038 29.29796110391662 20.97047808088352 74-75 20.677762050370802 29.102439265978262 29.185286526044035 21.034512157606898 76-77 20.674391582337684 29.01291243250138 29.172590511926526 21.140105473234406 78-79 20.57668241857875 29.07726171987012 29.195011544587967 21.15104431696317 80-81 20.72281145127137 28.952086816664835 29.137369114026097 21.187732618037703 82-83 20.71472611253833 28.969006381039197 29.32294687992044 20.99332062650203 84-85 20.85156211154388 28.94281925913649 29.066329659401674 21.139288969917956 86-87 20.57046490428441 29.04468384851247 29.147658904450154 21.237192342752962 88-89 20.7540508879435 28.922239393041377 29.080290485178402 21.243419233836725 90-91 20.706085819027095 28.841699884851728 29.26476660139885 21.187447694722326 92-93 20.729863190644554 28.896019717734557 29.127124218544854 21.246992873076035 94-95 20.428398554888723 28.936236793519704 29.11530939831174 21.520055253279835 96-97 20.410402126660543 28.832151569811003 29.210605391864785 21.54684091166367 98-99 20.36570812960968 28.824944062318718 29.1439131515704 21.6654346565012 100-101 20.386243473937185 28.818248114692963 29.038153642164584 21.75735476920527 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 216.0 1 162.5 2 142.0 3 203.5 4 192.5 5 222.0 6 390.0 7 774.0 8 1298.5 9 1664.5 10 1672.5 11 1558.0 12 1682.0 13 1889.5 14 2109.0 15 2453.0 16 2837.5 17 3230.5 18 3690.5 19 4134.0 20 4772.0 21 5677.0 22 6881.0 23 8800.5 24 11323.5 25 14528.0 26 19489.0 27 25901.0 28 33266.5 29 42556.0 30 52631.5 31 62447.5 32 73486.5 33 83892.5 34 92840.5 35 100615.0 36 109560.0 37 118293.0 38 121442.0 39 120621.5 40 119724.0 41 119584.5 42 119296.0 43 120156.5 44 120966.0 45 122477.0 46 122345.5 47 118911.5 48 115271.0 49 111348.5 50 105724.5 51 98091.0 52 88852.5 53 79380.5 54 70615.5 55 60091.5 56 48442.5 57 38096.0 58 29508.5 59 22529.5 60 16520.0 61 11353.5 62 7481.5 63 4629.0 64 2694.5 65 1508.0 66 800.5 67 415.0 68 215.0 69 120.5 70 67.5 71 37.5 72 20.5 73 14.0 74 10.5 75 4.0 76 1.5 77 1.0 78 0.5 79 2.0 80 1.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004707052291373167 2 0.007292616226071103 3 0.002254081378967432 4 0.16942073423386095 5 0.0018563023120908263 6 3.6463081130355516E-4 7 0.0 8 6.29816855887959E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.4861191679787854E-4 24-25 7.292616226071103E-4 26-27 0.003265103173945471 28-29 1.6574127786525236E-4 30-31 0.007309190353857628 32-33 0.022341924256236014 34-35 0.013889119085108148 36-37 0.001209911328416342 38-39 2.1546366122482806E-4 40-41 0.0022706555067539573 42-43 0.011983094389657745 44-45 0.008021877848678213 46-47 0.011071517361398856 48-49 0.010972072594679704 50-51 0.1894091323444104 52-53 0.11326758929311345 54-55 0.01823154056517776 56-57 0.007176597331565426 58-59 0.01864589375984089 60-61 0.12289715753708462 62-63 0.2375901218198392 64-65 0.24272810143366208 66-67 0.2537333222839148 68-69 0.24847932377558632 70-71 0.2352034474185796 72-73 0.15916134913400184 74-75 0.2718654180823734 76-77 0.20943067871053286 78-79 0.006132427281014337 80-81 6.629651114610094E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.3259302229220188E-4 90-91 0.006778818264688821 92-93 0.019292284743515374 94-95 0.0031988066627993703 96-97 4.972238335957571E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3016750.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.72104132322939 #Duplication Level Percentage of deduplicated Percentage of total 1 72.21239925098338 39.51537683462601 2 15.990606373967896 17.500452643467852 3 5.296891180824194 8.695542035715903 4 2.31110746109117 5.058648275231747 5 1.1998851578459395 3.282948265280864 6 0.7109381151504037 2.3341964386442435 7 0.48716178083373535 1.8660599958070598 8 0.31605868218672667 1.3836048166804236 9 0.23202661715157466 1.1427064294716398 >10 1.1208165514071995 11.056454715989695 >50 0.07985538224723407 3.002496198915788 >100 0.04043268090701396 3.8514235325105153 >500 0.001213843602791077 0.401658586571588 >1k 4.8553744061679476E-4 0.43792542730629047 >5k 1.2138436015419869E-4 0.4705058037803896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7571 0.2509654429435651 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6596 0.21864589375984086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3710 0.12298002817601723 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.94447667191514E-5 0.0 0.0 0.0 0.0 7 9.94447667191514E-5 0.0 0.0 0.0 0.0 8 9.94447667191514E-5 0.0 0.0 0.0 0.0 9 9.94447667191514E-5 0.0 0.0 3.314825557305047E-5 0.0 10-11 9.94447667191514E-5 0.0 0.0 3.314825557305047E-5 0.0 12-13 9.94447667191514E-5 0.0 0.0 3.314825557305047E-5 0.0 14-15 1.3259302229220188E-4 0.0 0.0 3.314825557305047E-5 0.0 16-17 2.3203778901135328E-4 0.0 0.0 9.94447667191514E-5 0.0 18-19 2.6518604458440376E-4 0.0 0.0 9.94447667191514E-5 0.0 20-21 2.6518604458440376E-4 0.0 0.0 1.1601889450567664E-4 0.0 22-23 2.6518604458440376E-4 0.0 0.0 1.6574127786525236E-4 0.0 24-25 2.6518604458440376E-4 0.0 0.0 2.6518604458440376E-4 0.0 26-27 2.6518604458440376E-4 0.0 0.0 4.4750145023618134E-4 0.0 28-29 2.6518604458440376E-4 0.0 0.0 8.287063893262618E-4 0.0 30-31 2.6518604458440376E-4 0.0 0.0 0.002336952017900058 0.0 32-33 2.6518604458440376E-4 0.0 0.0 0.006629651114610094 0.0 34-35 2.6518604458440376E-4 0.0 0.0 0.013690229551669843 0.0 36-37 2.6518604458440376E-4 0.0 0.0 0.025308693130024033 0.0 38-39 2.6518604458440376E-4 0.0 0.0 0.04476671915140466 0.0 40-41 2.6518604458440376E-4 0.0 0.0 0.06866661141957404 0.0 42-43 2.6518604458440376E-4 0.0 0.0 0.09286483798790088 0.0 44-45 2.6518604458440376E-4 0.0 0.0 0.12251595259799453 0.0 46-47 3.3148255573050466E-4 0.0 0.0 0.1592110715173614 0.0 48-49 3.3148255573050466E-4 0.0 0.0 0.20129278196734895 0.0 50-51 3.3148255573050466E-4 0.0 0.0 0.2451147758349217 0.0 52-53 3.3148255573050466E-4 0.0 0.0 0.29014668103091074 0.0 54-55 3.3148255573050466E-4 0.0 0.0 0.34306787105328584 0.0 56-57 3.3148255573050466E-4 0.0 0.0 0.40754122814286897 0.0 58-59 3.3148255573050466E-4 0.0 0.0 0.4809480401093893 3.314825557305047E-5 60-61 3.3148255573050466E-4 0.0 0.0 0.5622109886467225 6.629651114610094E-5 62-63 3.3148255573050466E-4 0.0 0.0 0.647932377558631 6.629651114610094E-5 64-65 3.3148255573050466E-4 0.0 0.0 0.7395873042181156 6.629651114610094E-5 66-67 3.3148255573050466E-4 0.0 0.0 0.8459269080964614 6.629651114610094E-5 68-69 3.3148255573050466E-4 0.0 0.0 0.9710118505013674 6.629651114610094E-5 70-71 3.6463081130355516E-4 0.0 0.0 1.10817933206265 6.629651114610094E-5 72-73 3.6463081130355516E-4 0.0 0.0 1.25389906356178 6.629651114610094E-5 74-75 3.6463081130355516E-4 0.0 0.0 1.4050882572304633 6.629651114610094E-5 76-77 3.6463081130355516E-4 0.0 0.0 1.5729675975801773 6.629651114610094E-5 78-79 3.6463081130355516E-4 0.0 0.0 1.7714593519516035 6.629651114610094E-5 80-81 3.6463081130355516E-4 0.0 0.0 1.9953260959642 6.629651114610094E-5 82-83 3.6463081130355516E-4 0.0 0.0 2.2340764067290957 1.3259302229220188E-4 84-85 3.6463081130355516E-4 0.0 0.0 2.474185795972487 1.3259302229220188E-4 86-87 3.977790668766056E-4 0.0 0.0 2.7333554321703817 1.3259302229220188E-4 88-89 4.3092732244965606E-4 0.0 0.0 3.024496560868484 1.3259302229220188E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4130 0.0 55.58564 1 GTATCAA 7980 0.0 41.39496 1 ATCAACG 9690 0.0 33.596115 3 CAACGCA 9785 0.0 33.16631 5 TCAACGC 9830 0.0 33.11599 4 AACGCAG 10250 0.0 31.985184 6 TATCAAC 10235 0.0 31.948595 2 ACGCAGA 11680 0.0 27.7036 7 CGCAGAG 11755 0.0 27.405758 8 TGGTATC 2080 0.0 25.363594 2 GTGGTAT 2340 0.0 24.577353 1 GCAGAGT 13455 0.0 23.237875 9 GAGTACT 9460 0.0 21.039507 12-13 GGCGTCG 300 1.6916601E-10 20.559702 8 AGAGTAC 12435 0.0 20.412804 10-11 CAGAGTA 13175 0.0 20.202585 10-11 TATACCG 330 2.7284841E-11 20.128714 5 GTACATG 7750 0.0 19.318544 1 ACGGGCG 325 4.965841E-10 18.9785 5 TACATGG 7720 0.0 18.962019 2 >>END_MODULE