FastQCFastQC Report
Sat 18 Jun 2016
SRR1931036_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931036_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733801
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN758964.377434319163503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.057.7610051
GTATCAA30100.048.6604041
ATCAACG37450.038.981233
TCAACGC38900.037.6576084
CAACGCA38900.037.3955575
TATCAAC39300.036.474452
AACGCAG43900.033.0413866
ACGCAGA49700.028.8104217
CGCAGAG49900.028.6225558
GTACATG32250.027.3133451
ACATGGG32400.026.1019923
GTGGTAT7300.025.9570311
TACATGG34650.024.969322
GCAGAGT54200.024.439099
TGGTATC7600.023.5764142
GAGTACT33100.022.56343312-13
AGAGTAC47400.020.67803610-11
CAGAGTA52600.020.63651810-11
AGTACTT37750.019.72048212-13
GAGTACA24300.019.5998551
Produced by FastQC (version 0.11.5)