##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931036_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1733801 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.244688404263233 30.0 2.0 31.0 2.0 33.0 2 19.908576013048787 27.0 2.0 31.0 2.0 33.0 3 20.570485886211856 27.0 2.0 31.0 2.0 33.0 4 22.101208270153265 30.0 2.0 35.0 2.0 37.0 5 21.45009202324834 30.0 2.0 35.0 2.0 37.0 6 22.457765337544505 30.0 2.0 35.0 2.0 37.0 7 22.652905956335243 30.0 2.0 35.0 2.0 37.0 8 23.10601620370504 32.0 2.0 35.0 2.0 37.0 9 24.426823493584326 32.0 2.0 37.0 2.0 39.0 10-11 24.794788444579282 32.5 2.0 37.0 2.0 39.0 12-13 24.70241077263192 32.5 2.0 37.0 2.0 39.0 14-15 24.6732332603338 32.5 2.0 38.0 2.0 40.0 16-17 23.509551269147956 31.5 2.0 38.0 2.0 40.0 18-19 22.454631759930926 30.0 2.0 38.0 2.0 40.0 20-21 22.10671668778597 29.0 2.0 38.0 2.0 40.0 22-23 21.740144341824696 27.0 2.0 37.0 2.0 40.0 24-25 21.5646896616163 27.0 2.0 37.0 2.0 40.0 26-27 21.28717194187799 27.0 2.0 37.0 2.0 39.5 28-29 21.085074930744646 27.0 2.0 37.0 2.0 39.0 30-31 21.08077974346537 27.0 2.0 37.0 2.0 39.0 32-33 20.949734427422754 26.5 2.0 37.0 2.0 39.0 34-35 20.617888385114554 25.0 2.0 36.0 2.0 39.0 36-37 20.14477065130312 25.0 2.0 36.0 2.0 39.0 38-39 20.03234454242442 24.0 2.0 36.0 2.0 39.0 40-41 19.903918615804237 24.0 2.0 35.0 2.0 39.0 42-43 19.763578980517373 24.0 2.0 35.0 2.0 39.0 44-45 19.357733961394647 23.5 2.0 35.0 2.0 39.0 46-47 19.00926922985971 22.0 2.0 35.0 2.0 38.5 48-49 18.77151501239185 20.0 2.0 35.0 2.0 38.0 50-51 16.500381820058934 11.5 2.0 33.0 2.0 37.5 52-53 18.400907024508577 20.5 2.0 34.0 2.0 38.0 54-55 19.182026080271037 23.0 2.0 36.0 2.0 39.0 56-57 19.16339447260672 22.5 2.0 36.0 2.0 39.0 58-59 18.886425258723463 21.5 2.0 36.0 2.0 39.0 60-61 18.596581153200397 19.5 2.0 35.0 2.0 39.0 62-63 18.162745320829785 17.5 2.0 34.5 2.0 39.0 64-65 17.81726045838017 13.5 2.0 34.0 2.0 38.0 66-67 17.459225424371077 12.0 2.0 34.0 2.0 38.0 68-69 17.01597847734544 8.0 2.0 34.0 2.0 38.0 70-71 16.67403785094137 7.5 2.0 33.0 2.0 37.5 72-73 16.272076495514767 4.5 2.0 32.5 2.0 36.5 74-75 15.826872864878956 2.0 2.0 32.0 2.0 36.0 76-77 15.415798583574471 2.0 2.0 31.5 2.0 36.0 78-79 15.044039367839792 2.0 2.0 31.0 2.0 35.0 80-81 14.611815023754168 2.0 2.0 31.0 2.0 35.0 82-83 14.153405725339875 2.0 2.0 30.5 2.0 34.5 84-85 13.751871177834134 2.0 2.0 30.0 2.0 34.0 86-87 13.2731394779447 2.0 2.0 29.5 2.0 34.0 88-89 12.857308595392436 2.0 2.0 29.0 2.0 34.0 90-91 12.368844232988677 2.0 2.0 29.0 2.0 34.0 92-93 11.819402861112664 2.0 2.0 28.5 2.0 34.0 94-95 11.247722201106125 2.0 2.0 26.5 2.0 34.0 96-97 10.610576992399935 2.0 2.0 25.5 2.0 33.5 98-99 9.923571390257589 2.0 2.0 24.5 2.0 33.0 100-101 8.972582493607975 2.0 2.0 10.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.7808911870118 1101 2 9.533025796691454 1101 3 8.777252094470239 1101 4 10.710749149307642 1101 5 11.740611586326551 1101 6 10.851822524903131 1101 7 10.739042149738768 1101 8 10.047162215065335 1101 9 9.854716986038436 1101 10-11 9.324511135094003 1101 12-13 9.074069891024706 1101 14-15 9.903243088977494 1101 16-17 10.977744332191197 1101 18-19 11.882899755184663 1101 20-21 11.886626196015367 1101 22-23 11.891217985160548 1101 24-25 11.620059154301305 1101 26-27 12.2217547660964 1101 28-29 11.985248717251519 1101 30-31 11.42164624207177 1101 32-33 11.212672587081837 1101 34-35 11.287986650942319 1101 36-37 11.685623663408755 1101 38-39 11.486683870410086 1101 40-41 11.239757021216228 1101 42-43 10.993355290887774 1101 44-45 10.73375701312736 1101 46-47 10.349751806327387 1101 48-49 10.435436628730987 1101 50-51 12.275081089661814 1101 52-53 11.429536368203916 1101 54-55 11.154038508306115 1101 56-57 11.060475731632447 1101 58-59 10.983518395238363 1101 60-61 10.722275979590023 1101 62-63 10.750700109397162 1101 64-65 10.687578134942477 1101 66-67 10.391589763234407 1101 68-69 10.372175755547339 1101 70-71 10.139385294982297 1101 72-73 9.967466799882855 1101 74-75 9.584703439711175 1101 76-77 9.368806222035042 1101 78-79 8.969518876319283 1101 80-81 8.935816875088383 1101 82-83 8.622488654558088 1101 84-85 8.48137871535104 1101 86-87 8.10888910497882 1101 88-89 8.01565908688623 1101 90-91 7.727481937731948 1101 92-93 7.473364205228222 1101 94-95 7.268118512373974 1101 96-97 6.848876155093274 1101 98-99 6.527760791986978 1101 100-101 6.014321477801149 1102 1 7.694819652129535 1102 2 9.26485176921178 1102 3 8.602230395869288 1102 4 10.607688158222086 1102 5 11.368084365157493 1102 6 10.415202179918875 1102 7 10.221757529895125 1102 8 9.549250920826033 1102 9 9.30880431311547 1102 10-11 8.610865143460106 1102 12-13 8.766168310238548 1102 14-15 9.197646573287134 1102 16-17 10.334243885685684 1102 18-19 11.124476624632717 1102 20-21 11.308419111461323 1102 22-23 11.466839881163938 1102 24-25 11.213646238542008 1102 26-27 11.447424446022449 1102 28-29 11.43528945108724 1102 30-31 11.203189114433858 1102 32-33 11.258727828429816 1102 34-35 11.132834134061277 1102 36-37 11.339134136704594 1102 38-39 10.976030875689819 1102 40-41 10.91120056568661 1102 42-43 10.559486359837624 1102 44-45 10.185636879645713 1102 46-47 10.101803538298743 1102 48-49 9.889895730393242 1102 50-51 11.64914381976882 1102 52-53 10.822108396592267 1102 54-55 10.448268717145357 1102 56-57 10.40144660693991 1102 58-59 10.638583334372615 1102 60-61 10.587273551226744 1102 62-63 10.648814759477848 1102 64-65 10.365815769873308 1102 66-67 10.09533132271364 1102 68-69 10.020524625183398 1102 70-71 9.723208948807361 1102 72-73 9.532360656907093 1102 74-75 9.17408475541407 1102 76-77 9.072806598039678 1102 78-79 8.750787421964679 1102 80-81 8.386264947281274 1102 82-83 8.410814807247304 1102 84-85 7.927516364382049 1102 86-87 7.756113094074683 1102 88-89 7.62813304073166 1102 90-91 7.526302536519335 1102 92-93 7.2756217997385555 1102 94-95 6.947385773336855 1102 96-97 6.593470931762189 1102 98-99 5.944823412425961 1102 100-101 5.137260267379553 1103 1 8.045792971258134 1103 2 9.430595808200195 1103 3 8.765759324621573 1103 4 10.653657337737837 1103 5 11.537955650712867 1103 6 10.852401563336063 1103 7 10.538885881389373 1103 8 10.058258520524546 1103 9 9.8855983050811 1103 10-11 9.282875641196764 1103 12-13 9.00734033078389 1103 14-15 9.836034232727155 1103 16-17 11.047714816576107 1103 18-19 11.528754337934078 1103 20-21 11.953189936434537 1103 22-23 12.23291110105436 1103 24-25 12.126148765900862 1103 26-27 11.83688147968532 1103 28-29 11.398513520076254 1103 30-31 11.1751720662777 1103 32-33 11.727690006775099 1103 34-35 11.845225179389267 1103 36-37 11.680083966080819 1103 38-39 11.209967384725552 1103 40-41 11.175533704663213 1103 42-43 10.845777841757194 1103 44-45 10.970966573994506 1103 46-47 10.97047192470301 1103 48-49 10.846071018728558 1103 50-51 12.335152021086941 1103 52-53 11.67624995713047 1103 54-55 11.248550482877278 1103 56-57 11.230946650531052 1103 58-59 11.165717739161828 1103 60-61 11.03593755996723 1103 62-63 10.803904628339609 1103 64-65 10.57711363588588 1103 66-67 10.308363714334 1103 68-69 10.114340494956558 1103 70-71 9.753172140569436 1103 72-73 9.687995105905765 1103 74-75 9.447174922303228 1103 76-77 9.125217373770356 1103 78-79 8.988123421880589 1103 80-81 8.533425092486157 1103 82-83 8.349773139678929 1103 84-85 8.014264904078738 1103 86-87 7.743722697976493 1103 88-89 7.450670713063525 1103 90-91 7.182123561795397 1103 92-93 6.985521193852577 1103 94-95 6.356772615983957 1103 96-97 5.7251903828724 1103 98-99 5.554214768765938 1103 100-101 4.796810654104101 1104 1 7.7069921310709475 1104 2 9.246946239085513 1104 3 8.727866496718871 1104 4 10.682597855427222 1104 5 11.522926211605338 1104 6 10.618020930084764 1104 7 10.234034970000902 1104 8 9.509888071287264 1104 9 9.290913863616272 1104 10-11 8.606557513145386 1104 12-13 8.604806807098157 1104 14-15 9.367458053384329 1104 16-17 10.08157220179493 1104 18-19 11.27881387329899 1104 20-21 11.3576950603947 1104 22-23 11.605103292667199 1104 24-25 11.383512913734261 1104 26-27 11.590389393819354 1104 28-29 11.332873293341468 1104 30-31 11.064076965239405 1104 32-33 11.10308580390992 1104 34-35 11.115051360239313 1104 36-37 11.093180872171093 1104 38-39 10.742669633142665 1104 40-41 10.429977454444664 1104 42-43 10.329153005592492 1104 44-45 10.351132284277032 1104 46-47 10.12636083141064 1104 48-49 9.826354413094435 1104 50-51 11.699806884779594 1104 52-53 11.020660837361593 1104 54-55 10.46264899988902 1104 56-57 10.085528281203189 1104 58-59 10.102238721534537 1104 60-61 9.841742845863589 1104 62-63 10.051638516411916 1104 64-65 9.869743971611076 1104 66-67 9.598196038630817 1104 68-69 9.213115995686657 1104 70-71 8.761210017722206 1104 72-73 8.82567583718694 1104 74-75 8.655268958638187 1104 76-77 8.336473265510643 1104 78-79 7.982893353114983 1104 80-81 7.939188296676482 1104 82-83 7.704501272565482 1104 84-85 7.278811729924383 1104 86-87 7.115193984145355 1104 88-89 6.987559578774652 1104 90-91 6.4288584615167395 1104 92-93 5.879208817369616 1104 94-95 5.575855825624409 1104 96-97 5.148140919384009 1104 98-99 4.9782140320346375 1104 100-101 4.546659780938846 1105 1 8.059268664094702 1105 2 9.289441739154743 1105 3 8.724215665311544 1105 4 10.664125358554397 1105 5 10.761944427893134 1105 6 10.87469982141543 1105 7 10.051537481796583 1105 8 9.910223924108458 1105 9 9.565123846184587 1105 10-11 9.05802334858225 1105 12-13 8.552254657012888 1105 14-15 9.671503079910554 1105 16-17 10.513020297859729 1105 18-19 10.965636590819305 1105 20-21 11.592377100789346 1105 22-23 11.941293978490965 1105 24-25 11.87006829808929 1105 26-27 12.089902419062756 1105 28-29 11.661641608148454 1105 30-31 11.582843540428183 1105 32-33 11.517158575571422 1105 34-35 11.329343773492127 1105 36-37 11.406731521687725 1105 38-39 11.181602229445915 1105 40-41 10.766757628240807 1105 42-43 10.415647384968903 1105 44-45 10.456113805271386 1105 46-47 10.569727679021266 1105 48-49 10.489501311426174 1105 50-51 11.955475732477794 1105 52-53 10.927685788457023 1105 54-55 10.91812217012427 1105 56-57 10.72548929363732 1105 58-59 10.752613774936084 1105 60-61 10.687725984584532 1105 62-63 10.612935650302166 1105 64-65 10.310916770136068 1105 66-67 10.032093209443577 1105 68-69 9.782999388013167 1105 70-71 9.665179690378128 1105 72-73 9.55937815892637 1105 74-75 9.35063814230755 1105 76-77 9.174849420670386 1105 78-79 8.633144887706758 1105 80-81 8.475156678228009 1105 82-83 8.154410770999233 1105 84-85 7.858570266183586 1105 86-87 7.6701003879521625 1105 88-89 7.340945118405402 1105 90-91 6.983078781204128 1105 92-93 6.912455051451225 1105 94-95 6.415221314676053 1105 96-97 6.147325399386093 1105 98-99 5.867533042511399 1105 100-101 5.174134299082354 1106 1 7.918935251725639 1106 2 9.130184746046837 1106 3 8.732173112712083 1106 4 10.460881282311856 1106 5 11.206353449333129 1106 6 10.351977049445939 1106 7 9.668380261971578 1106 8 9.271828367159664 1106 9 8.950886580927357 1106 10-11 8.739790723639931 1106 12-13 8.52557195822989 1106 14-15 9.201267284066649 1106 16-17 10.357054091997998 1106 18-19 11.419268963380123 1106 20-21 11.325197264313385 1106 22-23 11.648769130338724 1106 24-25 11.239767835034652 1106 26-27 11.69620044177827 1106 28-29 11.816355378925305 1106 30-31 11.279900924807354 1106 32-33 11.387231978154261 1106 34-35 11.117705037223107 1106 36-37 11.42328684270155 1106 38-39 10.958575687945796 1106 40-41 10.855090563435358 1106 42-43 10.64126982093294 1106 44-45 10.211065748884273 1106 46-47 9.997109681194605 1106 48-49 10.08640710705399 1106 50-51 11.857387953088715 1106 52-53 10.99873890845901 1106 54-55 11.017617791929723 1106 56-57 10.73360940071149 1106 58-59 10.409427468778901 1106 60-61 10.087325004067875 1106 62-63 10.188044457635261 1106 64-65 10.003970269627963 1106 66-67 9.35742424926071 1106 68-69 9.166950961312587 1106 70-71 8.963127662371654 1106 72-73 8.657638366449921 1106 74-75 8.434808825143685 1106 76-77 8.45610250106571 1106 78-79 8.001485128525959 1106 80-81 8.000941862669867 1106 82-83 7.732503251090074 1106 84-85 7.593945030223457 1106 86-87 7.137250090206516 1106 88-89 6.554360530490884 1106 90-91 6.368402971258121 1106 92-93 6.198522206955353 1106 94-95 6.002682488744405 1106 96-97 5.4965355897260295 1106 98-99 5.199450245037346 1106 100-101 4.555817813383824 1107 1 7.711911477219569 1107 2 8.531790436365352 1107 3 8.639045359459743 1107 4 10.349978608134034 1107 5 11.176570275828574 1107 6 10.278685604025814 1107 7 10.163307656385559 1107 8 8.697227314082763 1107 9 6.080526312841592 1107 10-11 8.147383401087371 1107 12-13 7.760983163315032 1107 14-15 8.586851521429669 1107 16-17 9.52773581456275 1107 18-19 10.970838793301045 1107 20-21 11.380722871098268 1107 22-23 11.008014140059704 1107 24-25 11.06217243875967 1107 26-27 11.181869906180967 1107 28-29 11.470313968369464 1107 30-31 10.957376932912272 1107 32-33 10.894688831416094 1107 34-35 11.134691849737685 1107 36-37 11.587736795766617 1107 38-39 11.30945209965597 1107 40-41 10.765632055438843 1107 42-43 10.574104233789246 1107 44-45 10.009685738829301 1107 46-47 10.46528035559274 1107 48-49 10.624833398116195 1107 50-51 12.043286759968755 1107 52-53 10.843942111519095 1107 54-55 10.877627471893256 1107 56-57 10.535415956132798 1107 58-59 10.508993100923881 1107 60-61 10.268382510785841 1107 62-63 10.430596833152219 1107 64-65 10.50471560338162 1107 66-67 10.510960219231727 1107 68-69 10.307153396772662 1107 70-71 10.025479049735438 1107 72-73 9.599457947633288 1107 74-75 9.775120043002632 1107 76-77 9.620051316793658 1107 78-79 9.273596843039693 1107 80-81 8.984547596348994 1107 82-83 8.730494160596477 1107 84-85 8.51486714050238 1107 86-87 8.084041053386327 1107 88-89 7.8010513540963995 1107 90-91 7.7281866193855855 1107 92-93 7.317137210430062 1107 94-95 7.095762779705822 1107 96-97 6.740078813810156 1107 98-99 6.304938262859729 1107 100-101 5.778827151150097 1108 1 6.580423681818566 1108 2 7.977005678141147 1108 3 7.8345333508904424 1108 4 9.310023846654339 1108 5 11.112394178149778 1108 6 9.499831899356224 1108 7 8.88368996097137 1108 8 5.979476533172786 1108 9 6.962816604820826 1108 10-11 5.881899680303672 1108 12-13 4.705675950939032 1108 14-15 7.23403131916902 1108 16-17 8.512694910865836 1108 18-19 8.149958596617918 1108 20-21 8.610668624546573 1108 22-23 9.23944528521153 1108 24-25 8.861608678349974 1108 26-27 7.412070469066244 1108 28-29 9.27782068063637 1108 30-31 9.5484935495797 1108 32-33 10.219093478720161 1108 34-35 9.675162064308523 1108 36-37 9.45882261943557 1108 38-39 9.223272543453206 1108 40-41 9.60994276017728 1108 42-43 8.66215190634497 1108 44-45 8.063989319988515 1108 46-47 8.937257151744024 1108 48-49 9.552751060975453 1108 50-51 11.076655419487338 1108 52-53 10.324180450960856 1108 54-55 9.72728844553076 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60-61 -16.266174724635327 2313 62-63 -15.844511158208473 2313 64-65 -15.50932327350823 2313 66-67 -15.159396152258552 2313 68-69 -14.704725652903363 2313 70-71 -14.373009071214888 2313 72-73 -13.981265594483341 2313 74-75 -13.536705010993048 2313 76-77 -13.140207862472002 2313 78-79 -12.78174872931452 2313 80-81 -12.372633829136968 2313 82-83 -11.903145148258814 2313 84-85 -11.511860721268677 2313 86-87 -11.034209486570477 2313 88-89 -10.626594434240532 2313 90-91 -10.151672991845516 2313 92-93 -9.615368189137975 2313 94-95 -9.065684304527437 2313 96-97 -8.443236520963064 2313 98-99 -7.760126531956686 2313 100-101 -6.831594015156599 2314 1 -18.90417923552341 2314 2 -17.56162209063249 2314 3 -18.2092267787692 2314 4 -19.69375789294588 2314 5 -19.033472920715703 2314 6 -20.06310705256166 2314 7 -20.264338131951373 2314 8 -20.746077221554387 2314 9 -22.038804140722124 2314 10-11 -22.442249428286278 2314 12-13 -22.3653667286936 2314 14-15 -22.342954094121094 2314 16-17 -21.131551442456693 2314 18-19 -20.07991714622379 2314 20-21 -19.74886676173111 2314 22-23 -19.37103553596621 2314 24-25 -19.194307042881793 2314 26-27 -18.91937199446698 2314 28-29 -18.724610437678056 2314 30-31 -18.699480518491615 2314 32-33 -18.57831332841112 2314 34-35 -18.275675320888666 2314 36-37 -17.792122815464484 2314 38-39 -17.672189369026533 2314 40-41 -17.55348241540349 2314 42-43 -17.402137666858703 2314 44-45 -17.006242986872643 2314 46-47 -16.66376932043318 2314 48-49 -16.450760554367605 2314 50-51 -14.222102008929735 2314 52-53 -16.077224195176367 2314 54-55 -16.844834731130504 2314 56-57 -16.827974972592905 2314 58-59 -16.546622449832057 2314 60-61 -16.266174724635327 2314 62-63 -15.844511158208473 2314 64-65 -15.50932327350823 2314 66-67 -15.159396152258552 2314 68-69 -14.704725652903363 2314 70-71 -14.373009071214888 2314 72-73 -13.981265594483341 2314 74-75 -13.536705010993048 2314 76-77 -13.140207862472002 2314 78-79 -12.78174872931452 2314 80-81 -12.372633829136968 2314 82-83 -11.903145148258814 2314 84-85 -11.511860721268677 2314 86-87 -11.034209486570477 2314 88-89 -10.626594434240532 2314 90-91 -10.151672991845516 2314 92-93 -9.615368189137975 2314 94-95 -9.065684304527437 2314 96-97 -8.443236520963064 2314 98-99 -7.760126531956686 2314 100-101 -6.831594015156599 2315 1 -18.90417923552341 2315 2 -17.56162209063249 2315 3 -18.2092267787692 2315 4 -19.69375789294588 2315 5 -19.033472920715703 2315 6 -20.06310705256166 2315 7 -20.264338131951373 2315 8 -20.746077221554387 2315 9 -22.038804140722124 2315 10-11 -22.442249428286278 2315 12-13 -22.3653667286936 2315 14-15 -22.342954094121094 2315 16-17 -21.131551442456693 2315 18-19 -20.07991714622379 2315 20-21 -19.74886676173111 2315 22-23 -19.37103553596621 2315 24-25 -19.194307042881793 2315 26-27 -18.91937199446698 2315 28-29 -18.724610437678056 2315 30-31 -18.699480518491615 2315 32-33 -18.57831332841112 2315 34-35 -18.275675320888666 2315 36-37 -17.792122815464484 2315 38-39 -17.672189369026533 2315 40-41 -17.55348241540349 2315 42-43 -17.402137666858703 2315 44-45 -17.006242986872643 2315 46-47 -16.66376932043318 2315 48-49 -16.450760554367605 2315 50-51 -14.222102008929735 2315 52-53 -16.077224195176367 2315 54-55 -16.844834731130504 2315 56-57 -16.827974972592905 2315 58-59 -16.546622449832057 2315 60-61 -16.266174724635327 2315 62-63 -15.844511158208473 2315 64-65 -15.50932327350823 2315 66-67 -15.159396152258552 2315 68-69 -14.704725652903363 2315 70-71 -14.373009071214888 2315 72-73 -13.981265594483341 2315 74-75 -13.536705010993048 2315 76-77 -13.140207862472002 2315 78-79 -12.78174872931452 2315 80-81 -12.372633829136968 2315 82-83 -11.903145148258814 2315 84-85 -11.511860721268677 2315 86-87 -11.034209486570477 2315 88-89 -10.626594434240532 2315 90-91 -10.151672991845516 2315 92-93 -9.615368189137975 2315 94-95 -9.065684304527437 2315 96-97 -8.443236520963064 2315 98-99 -7.760126531956686 2315 100-101 -6.831594015156599 2316 1 -18.90417923552341 2316 2 -17.56162209063249 2316 3 -18.2092267787692 2316 4 -19.69375789294588 2316 5 -19.033472920715703 2316 6 -20.06310705256166 2316 7 -20.264338131951373 2316 8 -20.746077221554387 2316 9 -22.038804140722124 2316 10-11 -22.442249428286278 2316 12-13 -22.3653667286936 2316 14-15 -22.342954094121094 2316 16-17 -21.131551442456693 2316 18-19 -20.07991714622379 2316 20-21 -19.74886676173111 2316 22-23 -19.37103553596621 2316 24-25 -19.194307042881793 2316 26-27 -18.91937199446698 2316 28-29 -18.724610437678056 2316 30-31 -18.699480518491615 2316 32-33 -18.57831332841112 2316 34-35 -18.275675320888666 2316 36-37 -17.792122815464484 2316 38-39 -17.672189369026533 2316 40-41 -17.55348241540349 2316 42-43 -17.402137666858703 2316 44-45 -17.006242986872643 2316 46-47 -16.66376932043318 2316 48-49 -16.450760554367605 2316 50-51 -14.222102008929735 2316 52-53 -16.077224195176367 2316 54-55 -16.844834731130504 2316 56-57 -16.827974972592905 2316 58-59 -16.546622449832057 2316 60-61 -16.266174724635327 2316 62-63 -15.844511158208473 2316 64-65 -15.50932327350823 2316 66-67 -15.159396152258552 2316 68-69 -14.704725652903363 2316 70-71 -14.373009071214888 2316 72-73 -13.981265594483341 2316 74-75 -13.536705010993048 2316 76-77 -13.140207862472002 2316 78-79 -12.78174872931452 2316 80-81 -12.372633829136968 2316 82-83 -11.903145148258814 2316 84-85 -11.511860721268677 2316 86-87 -11.034209486570477 2316 88-89 -10.626594434240532 2316 90-91 -10.151672991845516 2316 92-93 -9.615368189137975 2316 94-95 -9.065684304527437 2316 96-97 -8.443236520963064 2316 98-99 -7.760126531956686 2316 100-101 -6.831594015156599 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 437920.0 3 17550.0 4 66899.0 5 36092.0 6 20171.0 7 20354.0 8 19865.0 9 19136.0 10 20249.0 11 21795.0 12 22690.0 13 23289.0 14 23861.0 15 24561.0 16 25671.0 17 25626.0 18 25313.0 19 25636.0 20 27300.0 21 28767.0 22 30887.0 23 32945.0 24 35321.0 25 37733.0 26 40591.0 27 43699.0 28 46929.0 29 51362.0 30 55094.0 31 59893.0 32 64797.0 33 69999.0 34 73916.0 35 71704.0 36 56130.0 37 26221.0 38 3805.0 39 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54797362376825 21.403052530191893 14.364451359561384 22.684522486478475 2 14.735162450639248 21.06718601347565 42.3345844573901 21.863067078495003 3 18.341151072885502 27.075695993552053 30.55143153772515 24.031721395837298 4 11.876677419012639 17.549607538011976 40.47552406466422 30.098190978311163 5 11.98382226701801 38.64214666729305 36.27860891996122 13.09542214572772 6 28.51971783633492 39.792647096159214 18.351255142085822 13.336379925420042 7 25.151034592650483 33.6677011328543 23.905274260002578 17.275990014492642 8 23.7466168134817 37.688157080791285 21.019880231231877 17.545345874495137 9 24.97429807436533 17.06156893834871 22.25064557093568 35.71348741635028 10-11 23.38112596689905 27.91873274855715 29.501978801863427 19.19816248268037 12-13 24.022106551229232 25.525825668632407 29.870187481189436 20.581880298948928 14-15 21.41990683252105 27.166036219036886 27.544017686029903 23.870039262412163 16-17 20.503708941534075 30.26920256446173 28.308366110693722 20.918722383310477 18-19 20.246668747259346 28.881497078610852 30.734159512983293 20.137674661146512 20-21 20.91637490584591 28.263137976547753 30.81347916699694 20.007007950609395 22-23 20.73752255629222 28.048529622751694 30.469191085389586 20.7447567355665 24-25 20.576089307230465 28.36104247749614 30.738335740240935 20.32453247503246 26-27 20.492349576681963 28.400479868157436 30.544860401990565 20.56231015317004 28-29 20.427231607684327 28.483166223805107 30.70995008799113 20.37965208051944 30-31 20.706295318661745 28.312635160921 30.566284505788065 20.41478501462918 32-33 20.50011547339583 28.59689825519503 30.424357867933725 20.478628403475415 34-35 20.672022760853654 28.480862761037013 30.489130683910474 20.357983794198855 36-37 20.73537024975964 28.276310965742045 30.48062448259985 20.507694301898464 38-39 20.69432736115604 28.44347041772307 30.502644533011647 20.35955768810924 40-41 20.669485936043852 28.503402557695047 30.4822725749412 20.344838931319906 42-43 20.66077220014453 28.474043303930674 30.16771864173098 20.697465854193815 44-45 20.650670989000115 28.835367036880616 30.023209713074678 20.49075226104459 46-47 20.808097364425958 28.647346762104515 29.739124000241883 20.805431873227647 48-49 20.777399798239195 28.777986086131975 29.5801685129002 20.864445602728633 50-51 21.001202688248128 29.01385611365182 29.185127306345223 20.79981389175483 52-53 20.578182337518953 29.266346153134627 29.254691339844737 20.900780169501683 54-55 20.45269344148407 29.20535496348035 29.37527929551813 20.96667229951745 56-57 20.513514619824324 29.332830288936446 29.31356065070363 20.840094440535594 58-59 20.476755200981476 29.211008079643786 29.45033411183769 20.86190260753705 60-61 20.387606923752763 29.358818808691584 29.47756170104328 20.776012566512374 62-63 20.339511440306744 29.246073735270585 29.43862283965366 20.975791984769014 64-65 20.212228454581577 29.159219336221483 29.4874103529923 21.141141856204637 66-67 20.20921113245919 29.141507773320903 29.242742590907216 21.406538503312696 68-69 20.264776988767867 29.20267926114677 29.275260922155127 21.257282827930243 70-71 20.360359019671673 28.952946100544107 29.34704924894201 21.339645630842206 72-73 20.338687176774886 29.16487767772646 29.191322524621192 21.305112620877466 74-75 20.20545155965153 29.4105511442061 28.982619749570254 21.401377546572117 76-77 20.175555525195122 29.26180201410308 29.15110130222882 21.41154115847298 78-79 20.180863839277567 29.245847066939696 28.95854549701967 21.614743596763066 80-81 20.19890591756758 29.24719034200527 28.833515162479046 21.720388577948103 82-83 20.380538454057856 29.01543997187292 29.0243044906081 21.579717083461126 84-85 20.329990841584603 29.10576800240386 28.853290821972283 21.710950334039257 86-87 20.143916039681848 29.152244120113973 29.032558692456885 21.671281147747294 88-89 20.38617205674241 29.019055146465966 28.836935354524357 21.757837442267267 90-91 20.304615068653913 29.05432167585964 28.908688827602397 21.732374427884054 92-93 20.279283019310775 28.97859297160594 28.71531424749007 22.026809761593213 94-95 20.05028994617744 28.955378542352435 28.79146520192971 22.20286630954042 96-97 20.034140207886416 28.853407980488043 28.790324367716348 22.322127443909196 98-99 19.99995034046929 28.87148361749817 28.494887019246967 22.633679022785564 100-101 20.18210765654732 28.932090453346003 28.38470269608571 22.501099194020966 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 103260.0 1 94138.0 2 63148.5 3 32250.5 4 20020.0 5 15824.5 6 14842.5 7 15218.5 8 15686.5 9 15354.5 10 13894.5 11 11655.5 12 9183.5 13 7012.0 14 5413.5 15 4597.0 16 4504.5 17 4833.5 18 5539.5 19 6489.0 20 7555.5 21 8561.0 22 9705.5 23 11044.0 24 12548.0 25 14146.0 26 16011.5 27 18505.0 28 21143.5 29 24156.0 30 27471.5 31 30858.5 32 34630.0 33 38683.0 34 42303.0 35 45197.0 36 48789.0 37 51827.5 38 52962.5 39 53113.5 40 52366.0 41 51339.5 42 50372.0 43 49788.5 44 49712.5 45 49399.5 46 48018.0 47 45860.5 48 43895.5 49 42080.5 50 39658.0 51 36579.0 52 33249.5 53 29887.0 54 26508.5 55 22617.5 56 18699.0 57 15171.0 58 12240.0 59 9886.5 60 7733.0 61 5781.0 62 4105.5 63 2854.5 64 1928.5 65 1267.0 66 825.5 67 525.0 68 333.5 69 220.0 70 148.5 71 89.0 72 60.0 73 45.0 74 34.0 75 24.0 76 15.0 77 10.0 78 6.5 79 5.0 80 4.5 81 3.5 82 2.0 83 0.5 84 0.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.15369583937257 2 29.190489565988255 3 23.00165935998422 4 29.31893567946956 5 32.47523793099669 6 25.03101567019514 7 23.98723959670112 8 20.002007150762978 9 11.752213777705746 10-11 15.414514122439657 12-13 15.488167327161536 14-15 19.645334153112152 16-17 23.000563501809033 18-19 27.000561194739188 20-21 29.055785525559163 22-23 28.64333911446585 24-25 27.192394052143236 26-27 28.605416653929716 28-29 25.569831831911504 30-31 27.456495872363668 32-33 26.45144973385066 34-35 24.506099604279846 36-37 27.47212627054662 38-39 27.613261268161686 40-41 24.6189153195782 42-43 25.494534839926843 44-45 24.206699615469134 46-47 27.51163484159947 48-49 22.87309789301079 50-51 28.472846653104938 52-53 22.05720264320992 54-55 23.204854536362593 56-57 25.321129702889777 58-59 24.592182147778203 60-61 24.177861242437857 62-63 23.96099667724266 64-65 22.489259147964503 66-67 23.000678855301157 68-69 23.992084443370377 70-71 22.486058088558032 72-73 22.35567980408363 74-75 19.623734211711724 76-77 21.899831641578242 78-79 20.993528092324322 80-81 22.047368758006254 82-83 22.898302631040124 84-85 19.862054526442193 86-87 21.401648747462946 88-89 20.239923728271005 90-91 21.3658314881581 92-93 21.364447246252595 94-95 20.40623462554238 96-97 19.313865893490657 98-99 18.698945265344754 100-101 20.37396448612038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1733801.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.63950224332733 #Duplication Level Percentage of deduplicated Percentage of total 1 88.86397504708768 67.21626839925172 2 7.245538640885997 10.960978725628223 3 1.8197984713844193 4.129459516760562 4 0.7321911291777946 2.2153029023195265 5 0.38815035831506817 1.467974994926045 6 0.2255341658166287 1.0235575224740305 7 0.1453277878966312 0.7694765077037524 8 0.10195297026230339 0.6169337538295505 9 0.07559235333499137 0.5145991181694424 >10 0.38078420401846474 4.8842516659912345 >50 0.015798065159126733 0.8242042134252537 >100 0.0050417656446404445 0.6216281233745772 >500 7.876025404630564E-5 0.04724207425208108 >1k 1.5752050809261127E-4 0.18670449017152851 >5k 0.0 0.0 >10k+ 7.876025404630564E-5 4.521417991722504 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 75896 4.377434319163503 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.767674606255274E-5 0.0 3 0.0 0.0 0.0 5.767674606255274E-5 0.0 4 0.0 0.0 0.0 1.1535349212510548E-4 0.0 5 0.0 0.0 0.0 1.1535349212510548E-4 0.0 6 0.0 0.0 0.0 1.1535349212510548E-4 0.0 7 0.0 0.0 0.0 2.3070698425021097E-4 0.0 8 0.0 0.0 0.0 2.3070698425021097E-4 0.0 9 0.0 0.0 0.0 2.3070698425021097E-4 0.0 10-11 0.0 0.0 0.0 2.3070698425021097E-4 0.0 12-13 0.0 0.0 0.0 2.883837303127637E-4 0.0 14-15 0.0 0.0 0.0 3.4606047637531645E-4 0.0 16-17 0.0 0.0 0.0 3.4606047637531645E-4 0.0 18-19 0.0 0.0 0.0 4.3257559546914555E-4 0.0 20-21 0.0 0.0 0.0 4.6141396850042193E-4 0.0 22-23 0.0 0.0 0.0 4.6141396850042193E-4 0.0 24-25 0.0 0.0 0.0 5.190907145629746E-4 0.0 26-27 0.0 0.0 0.0 7.209593257819092E-4 0.0 28-29 0.0 0.0 0.0 9.228279370008438E-4 0.0 30-31 0.0 0.0 0.0 0.0026531303188774263 0.0 32-33 0.0 0.0 0.0 0.006834694408412499 0.0 34-35 0.0 0.0 0.0 0.012746560879824155 0.0 36-37 0.0 0.0 0.0 0.02200367862286387 0.0 38-39 0.0 0.0 0.0 0.035990289543032905 0.0 40-41 0.0 0.0 0.0 0.05127462724960938 0.0 42-43 0.0 0.0 0.0 0.06794320686168712 0.0 44-45 0.0 0.0 0.0 0.08821658310267441 0.0 46-47 0.0 0.0 0.0 0.11330596763988485 0.0 48-49 0.0 0.0 0.0 0.14381696630697527 0.0 50-51 0.0 0.0 0.0 0.17796159997600647 0.0 52-53 0.0 0.0 0.0 0.21507658606725916 0.0 54-55 0.0 0.0 0.0 0.2617370736318643 0.0 56-57 0.0 0.0 0.0 0.3129828625084424 0.0 58-59 0.0 0.0 0.0 0.3740048598426232 0.0 60-61 0.0 0.0 0.0 0.43917958289330783 0.0 62-63 0.0 0.0 0.0 0.5051906187618994 0.0 64-65 0.0 0.0 0.0 0.5774307432052468 0.0 66-67 0.0 0.0 0.0 0.657284198128851 0.0 68-69 0.0 0.0 0.0 0.7497111836940917 0.0 70-71 0.0 0.0 0.0 0.8571917999816587 0.0 72-73 0.0 0.0 0.0 0.9643551941658818 0.0 74-75 0.0 0.0 0.0 1.0690673266424464 0.0 76-77 0.0 0.0 0.0 1.1913131899220268 0.0 78-79 0.0 0.0 0.0 1.3296220269800283 0.0 80-81 0.0 0.0 0.0 1.484945504126483 0.0 82-83 0.0 0.0 0.0 1.6445947372276288 0.0 84-85 0.0 0.0 0.0 1.7987646794528322 0.0 86-87 0.0 0.0 0.0 1.9616726487065124 0.0 88-89 0.0 0.0 0.0 2.1448251558281486 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1525 0.0 57.761005 1 GTATCAA 3010 0.0 48.660404 1 ATCAACG 3745 0.0 38.98123 3 TCAACGC 3890 0.0 37.657608 4 CAACGCA 3890 0.0 37.395557 5 TATCAAC 3930 0.0 36.47445 2 AACGCAG 4390 0.0 33.041386 6 ACGCAGA 4970 0.0 28.810421 7 CGCAGAG 4990 0.0 28.622555 8 GTACATG 3225 0.0 27.313345 1 ACATGGG 3240 0.0 26.101992 3 GTGGTAT 730 0.0 25.957031 1 TACATGG 3465 0.0 24.96932 2 GCAGAGT 5420 0.0 24.43909 9 TGGTATC 760 0.0 23.576414 2 GAGTACT 3310 0.0 22.563433 12-13 AGAGTAC 4740 0.0 20.678036 10-11 CAGAGTA 5260 0.0 20.636518 10-11 AGTACTT 3775 0.0 19.720482 12-13 GAGTACA 2430 0.0 19.599855 1 >>END_MODULE