FastQCFastQC Report
Sat 18 Jun 2016
SRR1931036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733801
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54860.31641462889916433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.28157787427738246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.1709538753294063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.057.323591
GTATCAA51100.041.852171
ATCAACG63350.033.308133
CAACGCA64050.032.963075
TATCAAC64750.032.588892
TCAACGC65950.031.994074
AACGCAG67950.031.0693576
ACGCAGA77500.027.1795947
CGCAGAG77900.026.9182328
TAACGCA1801.9432264E-723.761364
GTGGTAT13250.023.314381
ACATGGG48500.023.0271383
GCAGAGT88300.022.780699
GTACATG50800.022.5465321
GGCGTGC3650.022.096218
TAGCGTA2806.730261E-1122.0265799
TACATGG52200.021.66872
CAGTCCG4750.020.9742979
TGGTATC14100.020.5606632
GAGTACA38200.020.5280151
Produced by FastQC (version 0.11.5)