##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931036_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1733801 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31494733247933 31.0 31.0 34.0 30.0 34.0 2 31.468448801217672 31.0 31.0 34.0 30.0 34.0 3 31.52821517579007 31.0 31.0 34.0 30.0 34.0 4 35.31461165381725 37.0 35.0 37.0 33.0 37.0 5 35.18666848156161 37.0 35.0 37.0 32.0 37.0 6 35.21800022032517 37.0 35.0 37.0 32.0 37.0 7 35.09689174247794 37.0 35.0 37.0 32.0 37.0 8 35.10560093113339 37.0 35.0 37.0 32.0 37.0 9 36.59429484698648 39.0 35.0 39.0 32.0 39.0 10-11 36.53819988568469 38.0 35.0 39.0 32.0 39.0 12-13 36.42215773321159 38.0 35.0 39.0 32.0 39.0 14-15 37.46800267158687 39.0 36.0 41.0 32.0 41.0 16-17 37.418369236146475 39.0 36.0 41.0 32.0 41.0 18-19 37.464279349244805 39.0 36.0 41.0 32.0 41.0 20-21 37.4580868854038 39.0 36.0 41.0 32.0 41.0 22-23 37.35818557031632 39.0 36.0 40.5 32.0 41.0 24-25 37.237091223271875 39.0 36.0 40.5 31.0 41.0 26-27 36.97985553128646 39.0 36.0 40.0 31.0 41.0 28-29 36.79136013879332 39.0 36.0 40.0 30.5 41.0 30-31 36.55834348924704 38.0 35.0 40.0 30.0 41.0 32-33 36.382454791524516 38.0 35.0 40.0 30.0 41.0 34-35 36.445783570317474 38.0 35.0 40.0 30.0 41.0 36-37 36.50247981169696 38.0 35.0 40.0 30.0 41.0 38-39 36.48369132328335 38.5 35.0 40.0 30.0 41.0 40-41 36.363637176354146 38.5 35.0 40.0 30.0 41.0 42-43 36.22137142613253 38.0 35.0 40.0 30.0 41.0 44-45 36.04063182568242 38.0 35.0 40.0 29.5 41.0 46-47 35.788037381452654 38.0 35.0 40.0 28.5 41.0 48-49 35.547822673997764 38.0 34.5 40.0 27.5 41.0 50-51 35.28738246200112 38.0 34.0 40.0 27.0 41.0 52-53 35.086573372607354 38.0 34.0 40.0 26.0 41.0 54-55 34.93452045534637 38.0 34.0 40.0 26.0 41.0 56-57 34.773706151974764 38.0 34.0 40.0 26.0 41.0 58-59 34.56826763855829 38.0 33.0 40.0 26.0 41.0 60-61 34.26966445399444 37.5 33.0 40.0 25.0 41.0 62-63 34.02522405973927 37.0 33.0 40.0 24.5 41.0 64-65 33.71449549285068 37.0 32.0 39.5 23.5 41.0 66-67 33.40679495513038 36.0 32.0 39.0 23.0 41.0 68-69 33.07336453260784 36.0 32.0 39.0 22.5 40.0 70-71 32.706269058559776 35.5 31.0 39.0 22.0 40.0 72-73 32.286295832105296 35.0 31.0 38.0 21.5 40.0 74-75 31.771218265533356 35.0 31.0 37.5 20.0 39.5 76-77 30.380699111374373 33.5 28.5 36.0 19.0 38.5 78-79 31.12174292205391 34.0 30.0 36.0 20.0 39.0 80-81 31.091343816274186 34.0 30.0 36.0 20.0 38.5 82-83 30.81750068202752 34.0 30.0 36.0 19.5 37.5 84-85 30.46396328067639 34.0 30.0 35.0 18.0 37.0 86-87 30.112755731482448 34.0 30.0 35.0 17.5 36.5 88-89 29.75806479521006 34.0 29.5 35.0 12.0 36.0 90-91 29.41506089799233 34.0 29.0 35.0 7.0 36.0 92-93 29.08515048728199 34.0 29.0 35.0 3.5 35.5 94-95 28.695198295536798 34.0 29.0 35.0 2.0 35.0 96-97 28.26497735322566 34.0 29.0 35.0 2.0 35.0 98-99 27.764373477694384 33.0 28.5 35.0 2.0 35.0 100-101 26.626558641966408 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3218012349570252 1101 2 0.3913519045555738 1101 3 0.2984697390816642 1101 4 0.20024731754092073 1101 5 0.15931545489890198 1101 6 0.28538067281074575 1101 7 0.2842559568656853 1101 8 0.39560934184678587 1101 9 0.478697662972543 1101 10-11 0.5850848706373881 1101 12-13 0.456635308873345 1101 14-15 0.3439725122543962 1101 16-17 0.5222204033783129 1101 18-19 0.516870894542663 1101 20-21 0.4929049138656012 1101 22-23 0.5154111061862068 1101 24-25 0.5317636137440047 1101 26-27 0.3576524319470451 1101 28-29 0.36282146875163335 1101 30-31 0.4505487897561622 1101 32-33 0.519363402088068 1101 34-35 0.4422850860220322 1101 36-37 0.5860248600079743 1101 38-39 0.5756822653394309 1101 40-41 0.577295692946393 1101 42-43 0.3604957718764439 1101 44-45 0.44956422648004235 1101 46-47 0.47797473902888044 1101 48-49 0.5731072280145924 1101 50-51 0.5640482827485656 1101 52-53 0.5327118209312189 1101 54-55 0.5000372669515016 1101 56-57 0.5658431656819118 1101 58-59 0.5162939068706791 1101 60-61 0.5520246460781024 1101 62-63 0.2645357330058218 1101 64-65 0.2742344073782377 1101 66-67 0.32226592177359237 1101 68-69 0.31185479001214844 1101 70-71 0.5482857098726797 1101 72-73 0.5313420553609589 1101 74-75 0.46346692774632103 1101 76-77 0.38348067530010965 1101 78-79 0.39351378544192883 1101 80-81 0.4679279415050246 1101 82-83 0.5548276947065602 1101 84-85 0.42606950767192586 1101 86-87 0.5850591740284834 1101 88-89 0.5753421235271716 1101 90-91 0.3320254943765448 1101 92-93 0.20559706653866172 1101 94-95 0.289088726091947 1101 96-97 0.6019996398180041 1101 98-99 0.4932962522167159 1101 100-101 0.7445194198052185 1102 1 0.2737716559255503 1102 2 0.5429084070857719 1102 3 0.46756309789975603 1102 4 0.08914296058718207 1102 5 0.3451667137310892 1102 6 0.39525737462340516 1102 7 0.35591868070594757 1102 8 0.3954645800613932 1102 9 0.46687513716138795 1102 10-11 0.5218938446147305 1102 12-13 0.5624528839567802 1102 14-15 0.702651952634163 1102 16-17 0.6972287851483472 1102 18-19 0.6677410319410271 1102 20-21 0.6557501722904533 1102 22-23 0.5265200065293101 1102 24-25 0.5777095004099451 1102 26-27 0.5755845631099987 1102 28-29 0.5365075675468489 1102 30-31 0.6166356781611171 1102 32-33 0.8915671026003764 1102 34-35 0.8005009440176067 1102 36-37 0.7309357939094951 1102 38-39 0.7119820663703038 1102 40-41 0.7889316264562396 1102 42-43 0.903304996523552 1102 44-45 0.7711111722257087 1102 46-47 0.9489999783401544 1102 48-49 0.938509161305106 1102 50-51 0.7072081317200372 1102 52-53 0.7975694875091079 1102 54-55 0.8910758230936935 1102 56-57 0.9157145407622025 1102 58-59 0.905093512065811 1102 60-61 0.7654563150686897 1102 62-63 0.4760123345505747 1102 64-65 0.6217449433415183 1102 66-67 0.5986976377647508 1102 68-69 0.5718981872140461 1102 70-71 0.5840000403494159 1102 72-73 0.5565738465530572 1102 74-75 0.4393018930442274 1102 76-77 0.4657298097061009 1102 78-79 0.584437503501114 1102 80-81 0.7089121336433948 1102 82-83 0.7008633524796757 1102 84-85 0.8187356938882289 1102 86-87 0.749548042724534 1102 88-89 0.764140632042114 1102 90-91 0.6371698644904455 1102 92-93 0.6273619383054125 1102 94-95 0.6906778156907158 1102 96-97 0.7432272949590839 1102 98-99 1.2310643513566077 1102 100-101 1.3752272485351078 1103 1 0.35460756711338703 1103 2 0.4734256701060744 1103 3 0.336382507521094 1103 4 0.21732503677461068 1103 5 0.33153319715007257 1103 6 0.3487830324941399 1103 7 0.4101565745797515 1103 8 0.40853326671897605 1103 9 0.455734044686956 1103 10-11 0.4646116813068488 1103 12-13 0.48019284083554936 1103 14-15 0.6097297013030882 1103 16-17 0.5126247751341353 1103 18-19 0.5651186691279193 1103 20-21 0.5714775167983674 1103 22-23 0.5768914278046253 1103 24-25 0.5889368477002748 1103 26-27 0.7462066365521096 1103 28-29 0.5068604888645254 1103 30-31 0.5198138744276122 1103 32-33 0.6960275895474552 1103 34-35 0.8003639874461399 1103 36-37 0.6460387957625784 1103 38-39 0.7705274453690194 1103 40-41 0.6960334893747699 1103 42-43 0.6772853811272768 1103 44-45 0.7445443797733375 1103 46-47 0.5419760229972752 1103 48-49 0.5813805253863791 1103 50-51 0.5832530052469806 1103 52-53 0.6475494850482164 1103 54-55 0.761936287534148 1103 56-57 0.6499152244939239 1103 58-59 0.6683223420733384 1103 60-61 0.6475971472328936 1103 62-63 0.7078167420170871 1103 64-65 0.6665846175889172 1103 66-67 0.5408917624205785 1103 68-69 0.37366189724699694 1103 70-71 0.49823724789458623 1103 72-73 0.4741194982282835 1103 74-75 0.5600841407519788 1103 76-77 0.6058920305913915 1103 78-79 0.5131633560171309 1103 80-81 0.39356174432192503 1103 82-83 0.47544133765373786 1103 84-85 0.4372552211747376 1103 86-87 0.5756342196328532 1103 88-89 0.6434967321125384 1103 90-91 0.6071201087554279 1103 92-93 0.43547109671192175 1103 94-95 0.6187615490217446 1103 96-97 0.678473424158291 1103 98-99 0.8313987974492036 1103 100-101 1.070613728850148 1104 1 0.29356286765996487 1104 2 0.36287889731472944 1104 3 0.3820324166656057 1104 4 0.1708021432413247 1104 5 0.18831854065857812 1104 6 0.3470265131456003 1104 7 0.3132953995958232 1104 8 0.283472224129639 1104 9 0.3844619231540207 1104 10-11 0.38098921208738545 1104 12-13 0.36757890974102736 1104 14-15 0.4777669273348977 1104 16-17 0.4679878715277752 1104 18-19 0.4657827823554399 1104 20-21 0.33503469908450256 1104 22-23 0.39958757497726083 1104 24-25 0.38552289393710026 1104 26-27 0.452840021693774 1104 28-29 0.5216407837740888 1104 30-31 0.494134839678253 1104 32-33 0.45925860846734423 1104 34-35 0.5259165057769977 1104 36-37 0.449175390086495 1104 38-39 0.31765684601217714 1104 40-41 0.22998778912432272 1104 42-43 0.34092579234634 1104 44-45 0.40830838339550013 1104 46-47 0.3666461820034215 1104 48-49 0.25530789404984944 1104 50-51 0.2572176023538333 1104 52-53 0.24699720787326385 1104 54-55 0.3396587267297022 1104 56-57 0.43526084311309177 1104 58-59 0.40061335937748055 1104 60-61 0.3878437472360261 1104 62-63 0.33134960975874606 1104 64-65 0.23896967363958055 1104 66-67 0.13439860943971382 1104 68-69 0.13787110482073928 1104 70-71 0.12509867006331632 1104 72-73 0.11382593914085959 1104 74-75 0.31794295964268926 1104 76-77 0.39956084394756886 1104 78-79 0.46718110388621525 1104 80-81 0.5039196618924215 1104 82-83 0.369749786912724 1104 84-85 0.28428068362133274 1104 86-87 0.4577560057782044 1104 88-89 0.557651860473797 1104 90-91 0.4136137440481136 1104 92-93 0.28938216323598454 1104 94-95 0.4868498699616737 1104 96-97 0.48284669612548115 1104 98-99 0.4995441517614907 1104 100-101 0.9831517561224707 1105 1 0.3244171267068694 1105 2 0.4114405762474824 1105 3 0.334019250004264 1105 4 0.09677221707450911 1105 5 0.3383830958784344 1105 6 0.3301962986991924 1105 7 0.2604953243307051 1105 8 0.35447704890748355 1105 9 0.3263956567178852 1105 10-11 0.38999454691655444 1105 12-13 0.5294449106191195 1105 14-15 0.5011519225953265 1105 16-17 0.5141455933616399 1105 18-19 0.5070115859359774 1105 20-21 0.36866465507334567 1105 22-23 0.4635830401357879 1105 24-25 0.48629101821447307 1105 26-27 0.5399701901053859 1105 28-29 0.583846187714613 1105 30-31 0.40093198362752247 1105 32-33 0.5480616801360583 1105 34-35 0.5571166174912534 1105 36-37 0.5999813540184107 1105 38-39 0.5820365064434441 1105 40-41 0.4464159683357707 1105 42-43 0.33438787517820145 1105 44-45 0.38607129946898056 1105 46-47 0.34410999025253375 1105 48-49 0.35689183417858317 1105 50-51 0.3431412804510998 1105 52-53 0.3717596375636987 1105 54-55 0.29872947475369216 1105 56-57 0.33276094074472695 1105 58-59 0.31436971578856543 1105 60-61 0.23626782473542818 1105 62-63 0.06442723696684993 1105 64-65 0.18711869619900057 1105 66-67 0.3574281663814389 1105 68-69 0.3572511616956078 1105 70-71 0.27818758703856616 1105 72-73 0.4276932997613514 1105 74-75 0.5331667195138401 1105 76-77 0.4896328310057605 1105 78-79 0.6442974618594093 1105 80-81 0.5585917497774631 1105 82-83 0.6339254563994672 1105 84-85 0.6352016161987031 1105 86-87 0.5837085059172544 1105 88-89 0.6537515654487507 1105 90-91 0.5812053964842292 1105 92-93 0.5706751729293273 1105 94-95 0.7262982571462508 1105 96-97 0.6585423658962455 1105 98-99 0.7812871545318636 1105 100-101 0.775396224550768 1106 1 0.27326768968102755 1106 2 0.15496756487669572 1106 3 0.2940825259493707 1106 4 0.1737766978378943 1106 5 0.13263207736838467 1106 6 0.20191479889115982 1106 7 0.20932546234129745 1106 8 0.1659342102105228 1106 9 0.36165151194811074 1106 10-11 0.31528639448475815 1106 12-13 0.22367817376753862 1106 14-15 0.3334056929992073 1106 16-17 0.18332882274966522 1106 18-19 0.3403979057690023 1106 20-21 0.3077887911935875 1106 22-23 0.3275609231563976 1106 24-25 0.3362064618254408 1106 26-27 0.3800934917622669 1106 28-29 0.3481845861429065 1106 30-31 0.32487815171194256 1106 32-33 0.3777845311412804 1106 34-35 0.4461399460558084 1106 36-37 0.44697676787405527 1106 38-39 0.4190038552150881 1106 40-41 0.3979556701727489 1106 42-43 0.3775763126359237 1106 44-45 0.4145106931258198 1106 46-47 0.21670247167543266 1106 48-49 0.34417995848313865 1106 50-51 0.3272367461387873 1106 52-53 0.2428384515778248 1106 54-55 0.3254268262279467 1106 56-57 0.38978743902680435 1106 58-59 0.3406279305461055 1106 60-61 0.46578787657339404 1106 62-63 0.12534809496924737 1106 64-65 0.20557339081766912 1106 66-67 0.1916576819930711 1106 68-69 0.2068852440330673 1106 70-71 0.18848899141761422 1106 72-73 0.2589114653095308 1106 74-75 0.5702985632456965 1106 76-77 0.3451831964497991 1106 78-79 0.5339770533838148 1106 80-81 0.4790202359117224 1106 82-83 0.5434899340132624 1106 84-85 0.5456745606703315 1106 86-87 0.6241039036105356 1106 88-89 0.5841477298182021 1106 90-91 0.6330038441277921 1106 92-93 0.46267225778380094 1106 94-95 0.6209646479279023 1106 96-97 0.7379914779875563 1106 98-99 0.7910616836550517 1106 100-101 0.8649247601489627 1107 1 0.25265147679683153 1107 2 0.32262224799852035 1107 3 0.2134218274216373 1107 4 0.13848203904488798 1107 5 0.32009025488672904 1107 6 0.3003348905578349 1107 7 0.30569969488777105 1107 8 0.20300811895870652 1107 9 0.2739417842879064 1107 10-11 0.2709580796539086 1107 12-13 0.24456187474807933 1107 14-15 0.3343878987417881 1107 16-17 0.32139662702689265 1107 18-19 0.37219456442371524 1107 20-21 0.36646756406484826 1107 22-23 0.41495526096367996 1107 24-25 0.2873163469901314 1107 26-27 0.3510985731571594 1107 28-29 0.39201776966727664 1107 30-31 0.3974936989521538 1107 32-33 0.39028117655342953 1107 34-35 0.40697784299583617 1107 36-37 0.4383311600110176 1107 38-39 0.433607006008053 1107 40-41 0.2353774013392993 1107 42-43 0.3067058835182195 1107 44-45 0.3008856756936851 1107 46-47 0.39972937048933943 1107 48-49 0.312365281736497 1107 50-51 0.4478967298023804 1107 52-53 0.4048356003246454 1107 54-55 0.26937532554061505 1107 56-57 0.3874529974587517 1107 58-59 0.46509778847565286 1107 60-61 0.2748803331160943 1107 62-63 0.2715690125969701 1107 64-65 0.3963102751116381 1107 66-67 0.17153264522444545 1107 68-69 0.3149529252599237 1107 70-71 0.16984656528592978 1107 72-73 0.12616881288233373 1107 74-75 0.3496756704835171 1107 76-77 0.4447790052365299 1107 78-79 0.40922242170877254 1107 80-81 0.24129392574741004 1107 82-83 0.3370150521123314 1107 84-85 0.6187886084537269 1107 86-87 0.5330079465773885 1107 88-89 0.5089720021509692 1107 90-91 0.5941279796988468 1107 92-93 0.4907919072894309 1107 94-95 0.7323205576862648 1107 96-97 0.91489061417386 1107 98-99 0.9476356797104053 1107 100-101 1.0199437802225795 1108 1 0.017403003319767407 1108 2 0.05345917812676859 1108 3 -0.0017376859112978593 1108 4 0.17600218167983428 1108 5 0.09507762380645346 1108 6 0.12665170255485236 1108 7 0.05414691797540172 1108 8 0.06648568581007908 1108 9 0.20957111546491092 1108 10-11 0.14659269663170704 1108 12-13 0.032064806442228644 1108 14-15 0.040384044657898244 1108 16-17 0.12027657400552982 1108 18-19 0.1095376677372073 1108 20-21 0.11785450335281666 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70-71 -1.625658264394211 2316 72-73 -2.3246228843140457 2316 74-75 -3.0361839263806587 2316 76-77 -2.249766672794369 2316 78-79 -2.0390841971320093 2316 80-81 -1.7224766887896799 2316 82-83 -1.3204070991266548 2316 84-85 -1.529305818936649 2316 86-87 -1.4211240598909178 2316 88-89 -1.4113380759252685 2316 90-91 -1.637787857278095 2316 92-93 -1.483005621280789 2316 94-95 -1.8692432019136298 2316 96-97 -1.703531730220405 2316 98-99 -1.772071267015452 2316 100-101 -2.035063770392881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 17.0 5 45.0 6 97.0 7 231.0 8 627.0 9 1197.0 10 2041.0 11 3388.0 12 4808.0 13 6270.0 14 7707.0 15 9649.0 16 11847.0 17 13480.0 18 11693.0 19 9049.0 20 8664.0 21 9026.0 22 9925.0 23 11606.0 24 14188.0 25 17924.0 26 23074.0 27 29944.0 28 38150.0 29 48097.0 30 59353.0 31 71338.0 32 86008.0 33 105428.0 34 129994.0 35 161099.0 36 210696.0 37 285678.0 38 276468.0 39 54966.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.73050980609303 22.551129999227097 15.144331431063183 22.574028763616692 2 14.891028732144793 22.18492814079743 41.14888202354776 21.775161103510015 3 18.200201757912424 27.253929463172838 31.335691597630817 23.210177181283925 4 12.132308758955396 18.4172636930899 40.08602958169632 29.364397966258377 5 11.597494709508926 39.14548115062485 36.72855296345365 12.52847117641257 6 28.37236048363328 40.765650801450924 17.8875448222481 12.974443892667695 7 24.757743247350763 34.01607220205779 23.827821070584225 17.39836348000722 8 23.581646901670272 38.409056696057014 20.76394532437338 17.24535107789933 9 24.511636571901853 16.8439169201079 22.157271797628447 36.4871747103618 10-11 23.24528593535244 28.19190322303425 29.4913026350775 19.071508206535814 12-13 23.77602735262005 25.67339042946682 30.046008740334095 20.50457347757903 14-15 21.042034235762927 27.37762292212313 27.679272304030277 23.901070538083665 16-17 19.964747972806567 30.62119009044291 28.615019832149137 20.79904210460139 18-19 19.828832720710164 29.090795310419132 31.23671632442247 19.843655644448237 20-21 20.415174521182074 28.43529909141822 31.34811895944229 19.801407427957418 22-23 20.276474837675213 28.17716076174052 31.2033591101588 20.34300529042547 24-25 20.171099152723222 28.547892189942264 31.2140743688682 20.066934288466307 26-27 20.074647160659527 28.5891934651723 31.159537109161523 20.17662226500665 28-29 20.080782236905474 28.547430869934598 31.2529667544701 20.118820138689824 30-31 20.34846312666245 28.413533264290187 31.164963227458525 20.073040381588843 32-33 20.274267524337976 28.70391389212344 30.89018540156976 20.131633181968827 34-35 20.347178618513368 28.563785404088488 31.120583511121097 19.968452466277046 36-37 20.423847980888024 28.361899016139287 31.198501311006805 20.01575169196589 38-39 20.190356662079434 28.58643279090182 31.213728473559204 20.009482073459537 40-41 20.22042177524431 28.663453624391284 31.140372379910335 19.975752220454073 42-43 20.307317989065023 28.630463053573425 30.791298973270543 20.270919984091016 44-45 20.122289039006343 28.966513130022015 30.71882663433466 20.19237119663698 46-47 20.374200407068113 28.755783069669675 30.44101737610365 20.428999147158557 48-49 20.48202674465388 28.967318338676208 30.13011809121678 20.420536825453134 50-51 20.449872579442815 29.1876000439182 29.89546313471907 20.467064241919918 52-53 20.48242235544248 29.30271474452622 29.764545831451862 20.450317068579434 54-55 20.488351070979235 29.24060877746672 29.805157983209757 20.465882168344287 56-57 20.352036472621307 29.313358143698622 29.80243227716239 20.532173106517682 58-59 20.326882030573984 29.258869339486587 30.031583501586383 20.382665128353043 60-61 20.37473092742873 29.408350742142215 29.89883881921355 20.318079511215508 62-63 20.298528345565323 29.297076001752988 29.950804166006602 20.453591486675094 64-65 20.159963504314348 29.15642176916768 29.940396740250684 20.743217986267286 66-67 20.15750736129198 29.206692125672813 29.81184468310014 20.823955829935073 68-69 20.240046073303272 29.25539112001841 29.84903013614985 20.65553267052847 70-71 20.35178406966882 28.988667774007627 29.8377319591205 20.82181619720305 72-73 20.373033144444335 29.235805451071894 29.715326236468076 20.675835168015695 74-75 20.24167174896814 29.41083763557744 29.628993438264423 20.718497177189995 76-77 20.17237447969905 29.37920507060603 29.702157961099424 20.7462624885955 78-79 20.16429596692162 29.359626994397242 29.533532464228973 20.942544574452164 80-81 20.224679893874725 29.353875879570886 29.535788441573423 20.885655784980965 82-83 20.29111184040152 29.22137546350475 29.698477506934186 20.78903518915954 84-85 20.449521023462324 29.221894554219315 29.39463640867666 20.933948013641704 86-87 20.14550689496661 29.30564118950214 29.60498350156679 20.943868413964463 88-89 20.29889277822862 29.170682414743577 29.47887846284373 21.05154634418407 90-91 20.2643424633356 29.194098395452954 29.585890968323202 20.95566817288824 92-93 20.34586983615948 29.150678878293977 29.440586287330518 21.06286499821602 94-95 19.976813399778518 29.167272286821706 29.39002514765596 21.465889165743818 96-97 20.08097240714834 29.059023803488348 29.50973886557306 21.350264923790252 98-99 20.058616877023372 29.082316828747935 29.380793989621644 21.478272304607046 100-101 20.0465624370963 29.04312547979843 29.274928322223833 21.63538376088144 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 260.0 1 195.5 2 179.5 3 251.5 4 213.0 5 202.5 6 369.5 7 819.5 8 1385.5 9 1696.0 10 1586.5 11 1363.0 12 1375.5 13 1533.0 14 1772.5 15 1978.0 16 2193.0 17 2450.0 18 2720.5 19 3026.0 20 3512.0 21 4202.5 22 4974.0 23 6193.0 24 7901.0 25 10047.0 26 13205.5 27 16984.0 28 21000.0 29 26162.5 30 31507.0 31 36498.5 32 43018.0 33 49893.5 34 55421.0 35 59994.5 36 66248.0 37 72327.5 38 74007.5 39 73276.5 40 71773.0 41 70023.0 42 68793.5 43 68919.0 44 69485.0 45 69851.0 46 67998.5 47 64569.5 48 62316.0 49 59784.5 50 56629.0 51 52422.0 52 47041.0 53 41391.5 54 35952.0 55 30167.5 56 24300.5 57 19148.0 58 15150.5 59 11885.0 60 8594.0 61 5932.0 62 4070.0 63 2576.0 64 1478.5 65 801.0 66 424.0 67 228.0 68 110.5 69 69.5 70 40.5 71 23.5 72 13.0 73 7.5 74 7.0 75 5.0 76 3.0 77 2.0 78 1.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004787169923191877 2 0.007671007226319515 3 0.0023070698425021094 4 0.17308791493372075 5 0.0017303023818765822 6 4.6141396850042193E-4 7 0.0 8 6.921209527506329E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.902523415316983E-4 24-25 6.344442066880801E-4 26-27 0.002566615199783597 28-29 2.3070698425021097E-4 30-31 0.005911866471411656 32-33 0.02004266925673708 34-35 0.013063782983168196 36-37 0.001095858175188502 38-39 1.7303023818765822E-4 40-41 0.001961009366126793 42-43 0.010929743378853744 44-45 0.007007724646600158 46-47 0.01064135964854098 48-49 0.010583682902478428 50-51 0.19096770621311213 52-53 0.11529581537904293 54-55 0.016956963342390503 56-57 0.006748179289318671 58-59 0.017360700564828375 60-61 0.12449525637602007 62-63 0.2412618287796581 64-65 0.24694298826681954 66-67 0.25868020609054904 68-69 0.25279717799216866 70-71 0.23832031473046794 72-73 0.16161024246727276 74-75 0.27462782637684485 76-77 0.2118178499147249 78-79 0.00579651297928655 80-81 5.767674606255274E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.1535349212510548E-4 90-91 0.005940704844442932 92-93 0.01825469012879794 94-95 0.0023647465885646623 96-97 2.883837303127637E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1733801.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.60530332463437 #Duplication Level Percentage of deduplicated Percentage of total 1 74.45991782868188 40.65906398562525 2 14.84458381047192 16.21186003397552 3 4.6224971580045855 7.572385782903022 4 2.0660684147643718 4.5127316991062 5 1.0452887623940497 2.8539154966179376 6 0.6575360367814697 2.1542972841198056 7 0.4206041074084476 1.6077050405237763 8 0.3012815342674885 1.3161255651829948 9 0.22567240839353586 1.1090619281096812 >10 1.2059238240663253 12.353034069716132 >50 0.10156050951244436 3.823298570083406 >100 0.04747619632839133 4.4930765800978145 >500 0.0010596059501116867 0.42127941948428493 >1k 4.238423800446747E-4 0.5947440122069723 >5k 1.0596059501116868E-4 0.3174205322470522 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5486 0.31641462889916433 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4882 0.28157787427738246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2964 0.1709538753294063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.1535349212510548E-4 0.0 8 0.0 0.0 0.0 1.1535349212510548E-4 0.0 9 0.0 0.0 0.0 1.1535349212510548E-4 0.0 10-11 0.0 0.0 0.0 1.1535349212510548E-4 0.0 12-13 0.0 0.0 0.0 1.1535349212510548E-4 0.0 14-15 0.0 0.0 0.0 1.1535349212510548E-4 0.0 16-17 0.0 0.0 0.0 1.1535349212510548E-4 0.0 18-19 0.0 0.0 0.0 1.7303023818765822E-4 0.0 20-21 0.0 0.0 0.0 1.7303023818765822E-4 0.0 22-23 0.0 0.0 0.0 1.7303023818765822E-4 0.0 24-25 0.0 0.0 0.0 2.3070698425021097E-4 0.0 26-27 0.0 0.0 0.0 5.47929087594251E-4 0.0 28-29 0.0 0.0 0.0 0.0012112116673136076 0.0 30-31 0.0 0.0 0.0 0.00484484666925443 0.0 32-33 0.0 0.0 0.0 0.013063782983168196 0.0 34-35 0.0 0.0 0.0 0.024426101957491085 0.0 36-37 0.0 0.0 0.0 0.04207518625263222 0.0 38-39 0.0 0.0 0.0 0.07123078138725264 0.0 40-41 0.0 0.0 0.0 0.10549076854840896 0.0 42-43 0.0 0.0 0.0 0.14087545225778508 0.0 44-45 0.0 0.0 0.0 0.18398881993954325 0.0 46-47 0.0 0.0 0.0 0.23863753683381195 0.0 48-49 0.0 0.0 0.0 0.3011014528195566 0.0 50-51 0.0 0.0 0.0 0.37034238646765116 0.0 52-53 0.0 0.0 0.0 0.4371320584080872 0.0 54-55 0.0 0.0 0.0 0.5166106144822848 0.0 56-57 0.0 0.0 0.0 0.6079994186183997 0.0 58-59 0.0 0.0 0.0 0.7132306418095271 0.0 60-61 0.0 0.0 0.0 0.8254407512742234 0.0 62-63 0.0 0.0 0.0 0.945177676100083 0.0 64-65 0.0 0.0 0.0 1.0726432848983245 0.0 66-67 0.0 0.0 0.0 1.2172677256501756 0.0 68-69 0.0 0.0 0.0 1.383549784548515 0.0 70-71 0.0 0.0 0.0 1.5759882477862224 0.0 72-73 0.0 0.0 0.0 1.7730985274549962 0.0 74-75 0.0 0.0 0.0 1.966229111645454 0.0 76-77 0.0 0.0 0.0 2.1872752409301874 0.0 78-79 0.0 0.0 0.0 2.445176810948892 0.0 80-81 0.0 0.0 0.0 2.7339931168571248 0.0 82-83 0.0 0.0 0.0 3.032210732373554 0.0 84-85 0.0 0.0 0.0 3.320248402209942 0.0 86-87 0.0 0.0 0.0 3.6325679821386654 0.0 88-89 0.0 0.0 0.0 3.9815988109362035 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2595 0.0 57.32359 1 GTATCAA 5110 0.0 41.85217 1 ATCAACG 6335 0.0 33.30813 3 CAACGCA 6405 0.0 32.96307 5 TATCAAC 6475 0.0 32.58889 2 TCAACGC 6595 0.0 31.99407 4 AACGCAG 6795 0.0 31.069357 6 ACGCAGA 7750 0.0 27.179594 7 CGCAGAG 7790 0.0 26.918232 8 TAACGCA 180 1.9432264E-7 23.76136 4 GTGGTAT 1325 0.0 23.31438 1 ACATGGG 4850 0.0 23.027138 3 GCAGAGT 8830 0.0 22.78069 9 GTACATG 5080 0.0 22.546532 1 GGCGTGC 365 0.0 22.09621 8 TAGCGTA 280 6.730261E-11 22.026579 9 TACATGG 5220 0.0 21.6687 2 CAGTCCG 475 0.0 20.974297 9 TGGTATC 1410 0.0 20.560663 2 GAGTACA 3820 0.0 20.528015 1 >>END_MODULE