FastQCFastQC Report
Sat 18 Jun 2016
SRR1931035_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931035_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763497
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN778284.413276574896356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.051.2066571
GTATCAA23800.044.3217281
TCAACGC28050.036.245524
ATCAACG28600.035.384493
TATCAAC29100.035.1890342
CAACGCA29750.033.9979745
AACGCAG31900.030.9598356
ACGCAGA37500.025.8356277
GTACATG26100.025.3090381
CGCAGAG38750.024.6403628
GTGGTAT7750.024.4470481
ACATGGG26800.023.4832463
TACATGG26850.023.2640782
GTATAGG5150.022.8689921
GCAGAGT42350.020.8861399
GAGTACA21200.020.5309721
TTCGCGC650.00577384419.14497216-17
AGAGTAC36950.019.09683810-11
CAGAGTA40900.018.59687610-11
GAGTACT27900.018.49787312-13
Produced by FastQC (version 0.11.5)