##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931035_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1763497 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.163692935116988 28.0 2.0 31.0 2.0 33.0 2 19.8879045442096 27.0 2.0 31.0 2.0 33.0 3 20.535952712139572 27.0 2.0 31.0 2.0 33.0 4 22.068937457789836 30.0 2.0 35.0 2.0 37.0 5 21.436650586873693 30.0 2.0 35.0 2.0 37.0 6 22.402867144089274 30.0 2.0 35.0 2.0 37.0 7 22.57641209483203 30.0 2.0 35.0 2.0 37.0 8 23.002488237859207 32.0 2.0 35.0 2.0 37.0 9 24.291050112361972 32.0 2.0 37.0 2.0 39.0 10-11 24.64751712081166 32.0 2.0 37.0 2.0 39.0 12-13 24.564660728087432 32.0 2.0 37.0 2.0 39.0 14-15 24.548492001971084 32.0 2.0 38.0 2.0 40.0 16-17 23.42708493408268 31.5 2.0 38.0 2.0 40.0 18-19 22.383438418097676 29.5 2.0 38.0 2.0 40.0 20-21 22.039813223385128 28.0 2.0 37.5 2.0 40.0 22-23 21.67100652850558 27.0 2.0 37.0 2.0 40.0 24-25 21.490330576122332 27.0 2.0 37.0 2.0 40.0 26-27 21.205295784455544 27.0 2.0 37.0 2.0 39.0 28-29 21.00550383697846 27.0 2.0 37.0 2.0 39.0 30-31 20.98914486386991 27.0 2.0 37.0 2.0 39.0 32-33 20.869180951257643 26.0 2.0 37.0 2.0 39.0 34-35 20.53185205305141 25.0 2.0 36.0 2.0 39.0 36-37 20.076338661194207 24.0 2.0 36.0 2.0 39.0 38-39 19.951741908265227 24.0 2.0 36.0 2.0 39.0 40-41 19.80471103721753 24.0 2.0 35.0 2.0 39.0 42-43 19.65230221542764 24.0 2.0 35.0 2.0 39.0 44-45 19.268649166967677 23.0 2.0 35.0 2.0 38.5 46-47 18.93486152797538 22.0 2.0 35.0 2.0 38.0 48-49 18.694190577018276 20.0 2.0 35.0 2.0 38.0 50-51 16.446431720609674 11.5 2.0 33.0 2.0 37.5 52-53 18.351759600384916 20.5 2.0 34.0 2.0 38.0 54-55 19.121684924896385 23.0 2.0 35.5 2.0 39.0 56-57 19.085790052378883 22.5 2.0 36.0 2.0 39.0 58-59 18.80878277649466 21.0 2.0 35.5 2.0 39.0 60-61 18.513594579406714 19.0 2.0 35.0 2.0 39.0 62-63 18.069485233034136 16.0 2.0 34.5 2.0 39.0 64-65 17.70940891875631 13.0 2.0 34.0 2.0 38.0 66-67 17.344833872697258 10.0 2.0 34.0 2.0 38.0 68-69 16.890214443234097 8.0 2.0 33.5 2.0 38.0 70-71 16.526242743820944 7.0 2.0 33.0 2.0 37.0 72-73 16.119241767919085 2.0 2.0 32.0 2.0 36.5 74-75 15.66183668018715 2.0 2.0 32.0 2.0 36.0 76-77 15.234869126513967 2.0 2.0 31.0 2.0 36.0 78-79 14.848998041958676 2.0 2.0 31.0 2.0 35.0 80-81 14.39859977079632 2.0 2.0 31.0 2.0 35.0 82-83 13.929857266556166 2.0 2.0 30.0 2.0 34.0 84-85 13.511690408319378 2.0 2.0 30.0 2.0 34.0 86-87 13.013372010272771 2.0 2.0 29.0 2.0 34.0 88-89 12.582404733322484 2.0 2.0 29.0 2.0 34.0 90-91 12.0865286416705 2.0 2.0 29.0 2.0 34.0 92-93 11.538434145337362 2.0 2.0 27.0 2.0 34.0 94-95 10.957718952739926 2.0 2.0 26.0 2.0 33.5 96-97 10.306037946194408 2.0 2.0 25.0 2.0 33.0 98-99 9.62913262682046 2.0 2.0 22.0 2.0 32.5 100-101 8.700465041902538 2.0 2.0 9.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.08046732253845 1101 2 9.731789985865785 1101 3 9.000428179289642 1101 4 10.863525286665297 1101 5 11.766611304622757 1101 6 10.935188222322896 1101 7 10.618696301044935 1101 8 10.043055710110721 1101 9 10.022561251023166 1101 10-11 9.485777920753545 1101 12-13 9.310801862989255 1101 14-15 9.851445032317251 1101 16-17 11.1030585298316 1101 18-19 11.897473277283805 1101 20-21 11.797672280173611 1101 22-23 11.934981796373524 1101 24-25 12.063069736158585 1101 26-27 12.01540955994108 1101 28-29 12.01973666878072 1101 30-31 11.561384628734782 1101 32-33 11.367020420510649 1101 34-35 11.48224080990429 1101 36-37 11.71747930864553 1101 38-39 11.25454555788625 1101 40-41 11.107955601539931 1101 42-43 10.754300867255388 1101 44-45 10.757509060758721 1101 46-47 10.63188679725673 1101 48-49 10.933593391914552 1101 50-51 12.436328537273493 1101 52-53 11.40819462388502 1101 54-55 11.288741922184688 1101 56-57 11.202338672903096 1101 58-59 10.997692846023085 1101 60-61 10.582328791656174 1101 62-63 10.971637314671728 1101 64-65 10.627425613645276 1101 66-67 10.288170192191675 1101 68-69 10.380731022413578 1101 70-71 10.129894369435771 1101 72-73 10.053071566594758 1101 74-75 9.727948918473308 1101 76-77 9.453061584565743 1101 78-79 9.27884970994571 1101 80-81 9.03591774058624 1101 82-83 8.66771525075903 1101 84-85 8.409328505669437 1101 86-87 8.07093793517605 1101 88-89 8.0899383911377 1101 90-91 7.726998909885568 1101 92-93 7.321685417918545 1101 94-95 6.852553495816078 1101 96-97 6.825642880124095 1101 98-99 6.485115502458003 1101 100-101 5.972380918878754 1102 1 7.673732680606392 1102 2 8.87756848232478 1102 3 8.522171280698558 1102 4 10.612793878798737 1102 5 11.016749800482852 1102 6 10.242628070202034 1102 7 10.122762838947885 1102 8 9.341661767994502 1102 9 9.382761283962719 1102 10-11 8.428936973891037 1102 12-13 8.727943907337878 1102 14-15 9.22778320177629 1102 16-17 10.064474331834923 1102 18-19 10.97932283334843 1102 20-21 11.026895655280295 1102 22-23 11.294450421446516 1102 24-25 11.184572528534577 1102 26-27 11.44819639609673 1102 28-29 10.943831953932463 1102 30-31 11.042305888223613 1102 32-33 10.928275267865754 1102 34-35 10.515893058028983 1102 36-37 10.64998615857591 1102 38-39 10.734107312067326 1102 40-41 10.598162072665417 1102 42-43 10.249044013581877 1102 44-45 9.944222441704685 1102 46-47 9.96058014496369 1102 48-49 9.708027804766967 1102 50-51 11.427966381828735 1102 52-53 10.509405152564891 1102 54-55 10.454990106765983 1102 56-57 10.1545763168265 1102 58-59 10.046044370974798 1102 60-61 10.181119760228842 1102 62-63 9.981043803764244 1102 64-65 9.486305069843151 1102 66-67 9.306705430030242 1102 68-69 9.13246933261436 1102 70-71 8.786319828717986 1102 72-73 8.698359712846381 1102 74-75 8.426058637139556 1102 76-77 7.958992201882966 1102 78-79 7.770877588788032 1102 80-81 7.2440103905735125 1102 82-83 7.219671411204612 1102 84-85 7.218626443952688 1102 86-87 6.857223101895121 1102 88-89 6.5302856040020885 1102 90-91 6.576174672361423 1102 92-93 6.388970449884884 1102 94-95 5.804018557428023 1102 96-97 5.490914107422006 1102 98-99 5.030379692668964 1102 100-101 4.624727862237691 1103 1 7.975905518166755 1103 2 9.296436225121756 1103 3 8.796581007025225 1103 4 10.870738548713934 1103 5 11.461522600391788 1103 6 10.677978381701923 1103 7 10.251854695294561 1103 8 9.708131859189763 1103 9 9.746566880015454 1103 10-11 9.109603404141776 1103 12-13 8.84168842927421 1103 14-15 9.796143307025162 1103 16-17 10.688621592693636 1103 18-19 11.552878835467421 1103 20-21 11.81756773324501 1103 22-23 11.88593682096317 1103 24-25 11.899928181283347 1103 26-27 12.046682896309068 1103 28-29 11.559638548185799 1103 30-31 11.406943318119723 1103 32-33 11.33269799706881 1103 34-35 11.490105333269668 1103 36-37 11.595521180969754 1103 38-39 11.502513824888513 1103 40-41 11.030907430813667 1103 42-43 10.365550223878227 1103 44-45 10.623488764254827 1103 46-47 10.509402662268023 1103 48-49 10.721809102515326 1103 50-51 12.370767735528862 1103 52-53 11.563929234154656 1103 54-55 11.089037503782766 1103 56-57 10.92495252462735 1103 58-59 10.75742517137408 1103 60-61 10.713441320924254 1103 62-63 10.895495822274736 1103 64-65 10.36905546277519 1103 66-67 10.322915682601263 1103 68-69 10.216479734915474 1103 70-71 9.878173986354877 1103 72-73 9.712193985276345 1103 74-75 9.441566646823853 1103 76-77 9.278760076459934 1103 78-79 8.853689274740113 1103 80-81 8.337867954351092 1103 82-83 7.95773569007034 1103 84-85 7.602116731198047 1103 86-87 7.399874287696155 1103 88-89 7.297872979391194 1103 90-91 7.103485966475981 1103 92-93 6.585461424487683 1103 94-95 6.43786456309325 1103 96-97 6.008879967209845 1103 98-99 5.8331974203615875 1103 100-101 5.109041664987995 1104 1 7.889573310166536 1104 2 9.539766210071289 1104 3 8.952354207527826 1104 4 10.999817018260895 1104 5 11.597477338527462 1104 6 10.663302450022758 1104 7 10.32889427526225 1104 8 9.795963936016179 1104 9 9.557745649190988 1104 10-11 8.966126884251683 1104 12-13 8.878786882113783 1104 14-15 9.34817615570583 1104 16-17 10.410344146302293 1104 18-19 11.20052228091983 1104 20-21 11.741305703226924 1104 22-23 11.73268655861558 1104 24-25 11.636928167475578 1104 26-27 11.521781449254956 1104 28-29 11.286910961645614 1104 30-31 11.149435344133558 1104 32-33 11.370598594945175 1104 34-35 11.024174275456541 1104 36-37 10.96004583128802 1104 38-39 10.645015171145918 1104 40-41 10.448786160637063 1104 42-43 10.287728347540604 1104 44-45 10.432747822150798 1104 46-47 10.094123244817762 1104 48-49 10.077328930470532 1104 50-51 11.559114755812477 1104 52-53 10.996953359777734 1104 54-55 10.926655991694066 1104 56-57 10.673179089392683 1104 58-59 10.678163403703586 1104 60-61 10.475082132544873 1104 62-63 10.386991531510752 1104 64-65 9.972270256418096 1104 66-67 10.134419365823447 1104 68-69 9.443175242558073 1104 70-71 9.119607560633767 1104 72-73 9.016029610699208 1104 74-75 8.616788520399927 1104 76-77 8.276938314036395 1104 78-79 8.0477915454276 1104 80-81 7.985615821050894 1104 82-83 7.4674369417445305 1104 84-85 7.175393694317501 1104 86-87 6.955624446485489 1104 88-89 6.639752458286019 1104 90-91 6.267341026332657 1104 92-93 5.9818462055655175 1104 94-95 5.750395199079051 1104 96-97 5.495756977966096 1104 98-99 4.891690147119663 1104 100-101 4.115290974599583 1105 1 7.898385467196693 1105 2 9.126418624312151 1105 3 8.555668174460418 1105 4 10.6295407746756 1105 5 10.692635071302263 1105 6 10.643265673872559 1105 7 10.066775822261903 1105 8 9.590282643381194 1105 9 9.45535053014655 1105 10-11 8.910393451307012 1105 12-13 8.369754323881171 1105 14-15 9.250404737044718 1105 16-17 10.146710400293422 1105 18-19 10.767681278847558 1105 20-21 11.149095176443037 1105 22-23 11.576710600968187 1105 24-25 11.484324931885869 1105 26-27 11.42528129805918 1105 28-29 11.155355068123018 1105 30-31 10.970468456009545 1105 32-33 11.230781408431842 1105 34-35 11.158610670949127 1105 36-37 11.159009195216264 1105 38-39 11.133178542633953 1105 40-41 10.95711221211478 1105 42-43 10.587476484274951 1105 44-45 10.278818010345073 1105 46-47 10.237248672835232 1105 48-49 10.083052691332174 1105 50-51 11.614283705980124 1105 52-53 10.462745592316846 1105 54-55 10.15733155890059 1105 56-57 9.996204983893605 1105 58-59 10.063804918507433 1105 60-61 9.574920819987618 1105 62-63 9.912759016858104 1105 64-65 9.63316146660691 1105 66-67 9.266854918621902 1105 68-69 9.276029140741056 1105 70-71 9.245677882236084 1105 72-73 9.01815024269757 1105 74-75 8.62552424573945 1105 76-77 8.48361016138202 1105 78-79 7.961072705099241 1105 80-81 7.998914101421237 1105 82-83 7.82637767077501 1105 84-85 7.685496574152429 1105 86-87 7.157892994650238 1105 88-89 6.754511723474522 1105 90-91 6.472645997116297 1105 92-93 6.234511031201208 1105 94-95 5.970165214732262 1105 96-97 5.526767399357629 1105 98-99 5.104439008704372 1105 100-101 4.547488282869992 1106 1 7.990975066976375 1106 2 9.244895261773241 1106 3 8.667609974388188 1106 4 10.621781204805114 1106 5 11.178805985228536 1106 6 10.498191785902588 1106 7 9.718586562636787 1106 8 9.139411285901726 1106 9 9.03957479029113 1106 10-11 8.481493890902367 1106 12-13 8.23501961200935 1106 14-15 8.922330936477096 1106 16-17 10.07301413831064 1106 18-19 11.40978980838268 1106 20-21 11.166416096443502 1106 22-23 11.499448295514004 1106 24-25 11.096174107307892 1106 26-27 11.362120180342497 1106 28-29 11.310821590548318 1106 30-31 10.852145734641095 1106 32-33 11.163115221653566 1106 34-35 11.098274875508011 1106 36-37 11.143876357382808 1106 38-39 10.833274547796737 1106 40-41 10.675516811347507 1106 42-43 10.41381323179704 1106 44-45 9.842315544664242 1106 46-47 9.677777006314233 1106 48-49 9.943562659323124 1106 50-51 11.582815011139115 1106 52-53 10.757690877894412 1106 54-55 10.33042807033738 1106 56-57 10.17246034828171 1106 58-59 10.158636333661462 1106 60-61 9.92310843051174 1106 62-63 10.064535400962306 1106 64-65 9.892575497048217 1106 66-67 9.25383155580814 1106 68-69 8.966082258284029 1106 70-71 8.564604394447954 1106 72-73 8.696912790294748 1106 74-75 8.379145534094102 1106 76-77 8.143744326741773 1106 78-79 7.722079492249222 1106 80-81 7.546268173848651 1106 82-83 7.6223874510432985 1106 84-85 7.300805975732155 1106 86-87 7.04809104068544 1106 88-89 6.757511713030219 1106 90-91 6.247526121216643 1106 92-93 6.078106720011455 1106 94-95 5.586748684253855 1106 96-97 5.3575017495266355 1106 98-99 4.841172128644036 1106 100-101 4.322024706838466 1107 1 7.309423144291596 1107 2 8.406285654173487 1107 3 8.314314372444478 1107 4 9.822799709770017 1107 5 10.692777852808224 1107 6 9.778331700596588 1107 7 10.005671726766074 1107 8 8.31420932368896 1107 9 5.478527246427472 1107 10-11 7.713237192226909 1107 12-13 7.770133806012094 1107 14-15 7.930953340258242 1107 16-17 8.902664444649027 1107 18-19 10.59983439617896 1107 20-21 10.529466636142264 1107 22-23 9.992747575266957 1107 24-25 10.266599246826317 1107 26-27 10.325972871672487 1107 28-29 10.426367977904551 1107 30-31 10.204598458292935 1107 32-33 10.612576933092765 1107 34-35 10.69871657927007 1107 36-37 10.897540840209217 1107 38-39 10.884136900991084 1107 40-41 10.330633492948223 1107 42-43 10.308033909941066 1107 44-45 9.525781958848174 1107 46-47 9.872244836623953 1107 48-49 10.051797708563178 1107 50-51 11.517390620438444 1107 52-53 10.410234221186595 1107 54-55 10.312345884274638 1107 56-57 9.97707503994507 1107 58-59 10.001950942320278 1107 60-61 9.743612724720684 1107 62-63 9.889383684279974 1107 64-65 9.854942257549787 1107 66-67 9.764120819453758 1107 68-69 9.592168784387308 1107 70-71 9.43554783995165 1107 72-73 8.814097692436535 1107 74-75 8.626999839824409 1107 76-77 8.439444702617264 1107 78-79 8.14469305518616 1107 80-81 7.8361183228647935 1107 82-83 7.6027640541972445 1107 84-85 7.480593029448761 1107 86-87 7.073528986256688 1107 88-89 6.980164282180223 1107 90-91 6.555889837372122 1107 92-93 6.038852311223518 1107 94-95 5.94236421103939 1107 96-97 5.7634783023645415 1107 98-99 5.3506907163878505 1107 100-101 4.818640501844598 1108 1 6.416401798380374 1108 2 8.04979329374487 1108 3 8.194613584739432 1108 4 9.348733671317678 1108 5 10.952559270042517 1108 6 9.641751195191247 1108 7 8.609812534285602 1108 8 6.0316769579553196 1108 9 7.316496806031317 1108 10-11 6.308510791922213 1108 12-13 5.156607190786001 1108 14-15 7.592072121735555 1108 16-17 8.784755518723223 1108 18-19 8.277719256803113 1108 20-21 8.634726684227527 1108 22-23 9.513807264696478 1108 24-25 8.956454271237352 1108 26-27 7.528419671259417 1108 28-29 9.438150822280381 1108 30-31 9.787888716663364 1108 32-33 10.331694735181447 1108 34-35 9.902100400994133 1108 36-37 9.596796266804382 1108 38-39 9.37882621148562 1108 40-41 9.472087987083796 1108 42-43 8.744675173715134 1108 44-45 7.915516004958789 1108 46-47 8.803815865537455 1108 48-49 9.457191062323112 1108 50-51 11.176263552698533 1108 52-53 10.490796587498348 1108 54-55 9.734652820590572 1108 56-57 9.49078279672569 1108 58-59 9.549155292211395 1108 60-61 9.675981065529793 1108 62-63 9.692599825375417 1108 64-65 9.53141882193609 1108 66-67 8.741993607752782 1108 68-69 9.163864651344614 1108 70-71 8.961207198610794 1108 72-73 8.734117829981694 1108 74-75 8.481974091198554 1108 76-77 8.064886079293077 1108 78-79 7.890656902823213 1108 80-81 8.061992801289337 1108 82-83 8.003589903255772 1108 84-85 7.698037142518272 1108 86-87 7.41237733178137 1108 88-89 7.291077380709666 1108 90-91 7.034019941896924 1108 92-93 6.868931784530076 1108 94-95 6.4622824139519395 1108 96-97 6.420812438252449 1108 98-99 6.1958285732724185 1108 100-101 5.660133848463158 1109 1 6.508119493358642 1109 2 8.077895313919278 1109 3 7.855459834614141 1109 4 8.707127828575999 1109 5 10.092658182957237 1109 6 9.122208946482903 1109 7 7.5473313281950105 1109 8 8.33921024026034 1109 9 7.402707316041067 1109 10-11 5.741770202545215 1109 12-13 6.147594656956965 1109 14-15 7.775304195763056 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50-51 -14.09019079974308 2313 52-53 -15.927993221418848 2313 54-55 -16.676175204887134 2313 56-57 -16.62970552599193 2313 58-59 -16.367776767236585 2313 60-61 -16.067118722155982 2313 62-63 -15.632506331737112 2313 64-65 -15.283071623923785 2313 66-67 -14.934757984603905 2313 68-69 -14.49192091128808 2313 70-71 -14.124249276973071 2313 72-73 -13.702453295283698 2313 74-75 -13.257410197548792 2313 76-77 -12.848595873997793 2313 78-79 -12.455680676794621 2313 80-81 -12.023198281513208 2313 82-83 -11.564881434323844 2313 84-85 -11.1862516048278 2313 86-87 -10.70917256206152 2313 88-89 -10.268901387867828 2313 90-91 -9.780735896744266 2313 92-93 -9.245717896998585 2313 94-95 -8.691645399211545 2313 96-97 -8.062202423743306 2313 98-99 -7.400243613564099 2313 100-101 -6.511044495485887 2314 1 -18.73168737359415 2314 2 -17.469314986509907 2314 3 -18.096256903583285 2314 4 -19.553601785157905 2314 5 -18.904659413609288 2314 6 -19.886911225190918 2314 7 -20.066939058071085 2314 8 -20.530424952872707 2314 9 -21.818322726877387 2314 10-11 -22.196266278141483 2314 12-13 -22.128424656347757 2314 14-15 -22.1101571776275 2314 16-17 -20.9737370502789 2314 18-19 -19.88788583873829 2314 20-21 -19.533266946345723 2314 22-23 -19.173029253350233 2314 24-25 -18.989140208592797 2314 26-27 -18.727684362710857 2314 28-29 -18.51009758536293 2314 30-31 -18.53005183535362 2314 32-33 -18.403974054627472 2314 34-35 -18.058090681808416 2314 36-37 -17.61180245931027 2314 38-39 -17.479984828854082 2314 40-41 -17.351160420559516 2314 42-43 -17.17995327639102 2314 44-45 -16.814314143661168 2314 46-47 -16.484135302190783 2314 48-49 -16.242649702008098 2314 50-51 -14.09019079974308 2314 52-53 -15.927993221418848 2314 54-55 -16.676175204887134 2314 56-57 -16.62970552599193 2314 58-59 -16.367776767236585 2314 60-61 -16.067118722155982 2314 62-63 -15.632506331737112 2314 64-65 -15.283071623923785 2314 66-67 -14.934757984603905 2314 68-69 -14.49192091128808 2314 70-71 -14.124249276973071 2314 72-73 -13.702453295283698 2314 74-75 -13.257410197548792 2314 76-77 -12.848595873997793 2314 78-79 -12.455680676794621 2314 80-81 -12.023198281513208 2314 82-83 -11.564881434323844 2314 84-85 -11.1862516048278 2314 86-87 -10.70917256206152 2314 88-89 -10.268901387867828 2314 90-91 -9.780735896744266 2314 92-93 -9.245717896998585 2314 94-95 -8.691645399211545 2314 96-97 -8.062202423743306 2314 98-99 -7.400243613564099 2314 100-101 -6.511044495485887 2315 1 -18.73168737359415 2315 2 -17.469314986509907 2315 3 -18.096256903583285 2315 4 -19.553601785157905 2315 5 -18.904659413609288 2315 6 -19.886911225190918 2315 7 -20.066939058071085 2315 8 -20.530424952872707 2315 9 -21.818322726877387 2315 10-11 -22.196266278141483 2315 12-13 -22.128424656347757 2315 14-15 -22.1101571776275 2315 16-17 -20.9737370502789 2315 18-19 -19.88788583873829 2315 20-21 -19.533266946345723 2315 22-23 -19.173029253350233 2315 24-25 -18.989140208592797 2315 26-27 -18.727684362710857 2315 28-29 -18.51009758536293 2315 30-31 -18.53005183535362 2315 32-33 -18.403974054627472 2315 34-35 -18.058090681808416 2315 36-37 -17.61180245931027 2315 38-39 -17.479984828854082 2315 40-41 -17.351160420559516 2315 42-43 -17.17995327639102 2315 44-45 -16.814314143661168 2315 46-47 -16.484135302190783 2315 48-49 -16.242649702008098 2315 50-51 -14.09019079974308 2315 52-53 -15.927993221418848 2315 54-55 -16.676175204887134 2315 56-57 -16.62970552599193 2315 58-59 -16.367776767236585 2315 60-61 -16.067118722155982 2315 62-63 -15.632506331737112 2315 64-65 -15.283071623923785 2315 66-67 -14.934757984603905 2315 68-69 -14.49192091128808 2315 70-71 -14.124249276973071 2315 72-73 -13.702453295283698 2315 74-75 -13.257410197548792 2315 76-77 -12.848595873997793 2315 78-79 -12.455680676794621 2315 80-81 -12.023198281513208 2315 82-83 -11.564881434323844 2315 84-85 -11.1862516048278 2315 86-87 -10.70917256206152 2315 88-89 -10.268901387867828 2315 90-91 -9.780735896744266 2315 92-93 -9.245717896998585 2315 94-95 -8.691645399211545 2315 96-97 -8.062202423743306 2315 98-99 -7.400243613564099 2315 100-101 -6.511044495485887 2316 1 -18.73168737359415 2316 2 -17.469314986509907 2316 3 -18.096256903583285 2316 4 -19.553601785157905 2316 5 -18.904659413609288 2316 6 -19.886911225190918 2316 7 -20.066939058071085 2316 8 -20.530424952872707 2316 9 -21.818322726877387 2316 10-11 -22.196266278141483 2316 12-13 -22.128424656347757 2316 14-15 -22.1101571776275 2316 16-17 -20.9737370502789 2316 18-19 -19.88788583873829 2316 20-21 -19.533266946345723 2316 22-23 -19.173029253350233 2316 24-25 -18.989140208592797 2316 26-27 -18.727684362710857 2316 28-29 -18.51009758536293 2316 30-31 -18.53005183535362 2316 32-33 -18.403974054627472 2316 34-35 -18.058090681808416 2316 36-37 -17.61180245931027 2316 38-39 -17.479984828854082 2316 40-41 -17.351160420559516 2316 42-43 -17.17995327639102 2316 44-45 -16.814314143661168 2316 46-47 -16.484135302190783 2316 48-49 -16.242649702008098 2316 50-51 -14.09019079974308 2316 52-53 -15.927993221418848 2316 54-55 -16.676175204887134 2316 56-57 -16.62970552599193 2316 58-59 -16.367776767236585 2316 60-61 -16.067118722155982 2316 62-63 -15.632506331737112 2316 64-65 -15.283071623923785 2316 66-67 -14.934757984603905 2316 68-69 -14.49192091128808 2316 70-71 -14.124249276973071 2316 72-73 -13.702453295283698 2316 74-75 -13.257410197548792 2316 76-77 -12.848595873997793 2316 78-79 -12.455680676794621 2316 80-81 -12.023198281513208 2316 82-83 -11.564881434323844 2316 84-85 -11.1862516048278 2316 86-87 -10.70917256206152 2316 88-89 -10.268901387867828 2316 90-91 -9.780735896744266 2316 92-93 -9.245717896998585 2316 94-95 -8.691645399211545 2316 96-97 -8.062202423743306 2316 98-99 -7.400243613564099 2316 100-101 -6.511044495485887 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 448378.0 3 18348.0 4 66923.0 5 35951.0 6 20947.0 7 20928.0 8 20401.0 9 20004.0 10 21159.0 11 22274.0 12 23352.0 13 24077.0 14 24675.0 15 24987.0 16 26083.0 17 26773.0 18 26355.0 19 26939.0 20 28300.0 21 30179.0 22 31706.0 23 34109.0 24 36139.0 25 38859.0 26 41645.0 27 44676.0 28 48383.0 29 51900.0 30 56409.0 31 61135.0 32 65890.0 33 71502.0 34 73859.0 35 70736.0 36 53401.0 37 23168.0 38 2935.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.16749878470317 21.39496456565037 14.672792683077473 22.764743966568982 2 14.596182389671252 21.047661508301424 42.18919606546708 22.166960036560244 3 18.378938357044422 26.578444825740572 30.77955479394224 24.26306202327277 4 12.026265847050404 17.745261859728046 40.51860750434516 29.709864788876395 5 12.298481193804546 38.38895164717542 36.38989844846487 12.922668710555167 6 28.891093291491593 39.47534379879755 18.492716670564185 13.14084623914667 7 25.412228931739598 33.421375615434876 23.652473596607756 17.51392185621776 8 23.75321616670402 37.726952307756484 21.076771551940517 17.443059973598974 9 24.74852390275487 17.40593573517094 22.080179444093297 35.76536091798089 10-11 23.30007026654084 27.939770962544486 29.629013419603726 19.131145351310945 12-13 23.866582742107607 25.339485566584617 30.01620172997206 20.777729961335716 14-15 21.21006923036271 27.2297637712051 27.50770694241373 24.05246005601846 16-17 20.633825051949863 30.427530857851952 28.228491287842633 20.710152802355548 18-19 20.45095846948085 29.279582571622715 30.27260699754074 19.9968519613557 20-21 20.991009377316132 28.505950981433543 30.4242607968829 20.078778844367424 22-23 20.71390259261486 28.34903152718401 30.13325401170205 20.803811868499082 24-25 20.658666149670417 28.577045366421096 30.268321054672352 20.49596742923614 26-27 20.687448965267713 28.70250680627858 30.095673440981606 20.514370787472096 28-29 20.641968354773905 28.787423893978964 30.122327583163894 20.448280168083237 30-31 20.875199717341108 28.607344617379322 30.100954051586804 20.41650161369277 32-33 20.635956088106376 28.720731291524476 30.01100408218429 20.63230853818486 34-35 20.853823262686664 28.672551996918976 30.069566265357857 20.4040584750365 36-37 20.86484285211498 28.608242204898044 29.916536112400216 20.61037883058676 38-39 20.903380062129166 28.72181348227073 29.963967891222882 20.410838564377222 40-41 20.924507258737197 28.668984714008566 30.03248390331681 20.37402412393743 42-43 20.81077362046005 28.69011701672366 29.77434790536312 20.724761457453166 44-45 20.88314570461163 28.88924269038446 29.655541929640815 20.5720696753631 46-47 20.93164333002762 28.86804743565633 29.347901843997477 20.852407390318575 48-49 20.974419750046174 28.96892580833355 29.362795108808776 20.693859332811495 50-51 21.207316735986854 29.069055546997507 28.959012216942565 20.764615500073074 52-53 20.69367331551169 29.31490385051287 29.160659930417964 20.830762903557478 54-55 20.68154354958119 29.259030851268946 29.204315144807126 20.855110454342743 56-57 20.6666401871722 29.391860581678834 29.177754954975406 20.76374427617356 58-59 20.71296409400173 29.254287129580987 29.261893538748847 20.770855237668435 60-61 20.70625010939265 29.372456737302354 29.234519590314754 20.686773562990243 62-63 20.680682451113753 29.30997261917015 29.254014511208155 20.75533041850794 64-65 20.5101382270044 29.183983023346542 29.252312595028783 21.053566154620267 66-67 20.475984577979283 29.247589208109186 29.05756683267166 21.21885938123987 68-69 20.54186635504501 29.239582195044534 29.06656229719875 21.15198915271171 70-71 20.63385467935397 29.160304786904373 29.10940974880601 21.096430784935652 72-73 20.606765102253906 29.20076616276992 29.030552250794205 21.16191648418197 74-75 20.556799590084466 29.37842967298608 28.769574188271868 21.295196548657586 76-77 20.540272506980976 29.26114739590968 28.883930268036984 21.31464982907236 78-79 20.36397857349884 29.2997384306555 28.902267530692356 21.434015465153312 80-81 20.44216223154397 29.435772333551007 28.626148363257165 21.49591707164786 82-83 20.641774492765947 29.20158966687214 28.756126718907932 21.40050912145398 84-85 20.570426613133446 29.300333571052256 28.663326839289894 21.465912976524404 86-87 20.512318751171335 29.28183214871927 28.77869342207357 21.42715567803582 88-89 20.645603976917094 29.200966017149852 28.63817667452778 21.51525333140527 90-91 20.593844422755108 29.234264596713167 28.674895432837054 21.49699554769467 92-93 20.571784096349514 29.19045476176441 28.603078710259922 21.63468243162615 94-95 20.41962607394694 29.107425241074182 28.676319105919195 21.796629579059687 96-97 20.502412689580353 29.03728237000367 28.53985238986092 21.92045255055505 98-99 20.297201075885706 29.081374381019543 28.353853813281432 22.267570729813315 100-101 20.476035086211763 29.194823068726183 28.31575034007832 22.01339150498373 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 105222.0 1 95430.5 2 63401.0 3 32235.0 4 19987.0 5 15700.5 6 14661.5 7 14970.0 8 15426.5 9 15053.0 10 13631.5 11 11483.0 12 9081.5 13 6913.5 14 5340.5 15 4377.0 16 4129.5 17 4522.5 18 5331.5 19 6257.0 20 7212.0 21 8342.0 22 9472.0 23 10665.5 24 12178.5 25 14007.5 26 16327.5 27 18970.0 28 21936.5 29 25479.5 30 29110.5 31 32854.5 32 36750.0 33 40268.0 34 43228.5 35 45433.5 36 47690.0 37 50445.0 38 52038.5 39 52521.5 40 52489.5 41 52231.5 42 51762.0 43 51458.0 44 51615.5 45 51378.5 46 50521.5 47 48978.0 48 46962.0 49 44524.0 50 41426.5 51 37820.5 52 34047.0 53 30433.5 54 26988.5 55 23057.5 56 19086.0 57 15491.5 58 12401.0 59 9894.5 60 7584.5 61 5589.0 62 4030.0 63 2818.0 64 1885.5 65 1240.0 66 812.0 67 516.5 68 319.0 69 209.5 70 150.5 71 97.5 72 67.0 73 43.5 74 28.0 75 20.0 76 14.0 77 11.0 78 8.0 79 2.5 80 1.5 81 1.5 82 1.5 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.96147767759174 2 28.901438448718654 3 22.765902068446955 4 29.00651376214419 5 32.08284448456675 6 24.82947234954185 7 23.720482654634516 8 19.79918310039654 9 11.720405535138422 10-11 15.304732585312026 12-13 15.405810160153377 14-15 19.524955245174784 16-17 22.773330490497006 18-19 26.68722997544084 20-21 28.73197402660736 22-23 28.321284357160803 24-25 26.87824249204847 26-27 28.29829027211274 28-29 25.30049668357814 30-31 27.136762920492636 32-33 26.155587449255655 34-35 24.245944280029963 36-37 27.17492572995588 38-39 27.284906070154925 40-41 24.369732412360214 42-43 25.205458245746943 44-45 23.958447335039413 46-47 27.182212388226347 48-49 22.630319189655555 50-51 28.21847726420856 52-53 21.822350704310807 54-55 22.946168890562333 56-57 25.047930333876383 58-59 24.33210830525938 60-61 23.864883240515862 62-63 23.694256355412 64-65 22.24001515171276 66-67 22.755298137734286 68-69 23.71855466723221 70-71 22.23122579737873 72-73 22.09459386661843 74-75 19.433914545927777 76-77 21.66516302551124 78-79 20.763403623595618 80-81 21.804601879107253 82-83 22.636046446350633 84-85 19.65172041687624 86-87 21.180784543438406 88-89 20.003209531969716 90-91 21.130997104049513 92-93 21.116849078847313 94-95 20.17803262494918 96-97 19.084948826110846 98-99 18.50615566683697 100-101 20.15118256509651 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1763497.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.81769095985045 #Duplication Level Percentage of deduplicated Percentage of total 1 90.63472284533768 73.24889021146198 2 6.480768380365805 10.475214722935483 3 1.443270072140804 3.4992526398562975 4 0.5857434962865511 1.8935374745851516 5 0.2741337750415193 1.1077429356481336 6 0.1575463901039344 0.7639521280355888 7 0.10286638082533991 0.5819396362990426 8 0.06997850484213494 0.4524400942531248 9 0.045861457597409665 0.33357753963681663 >10 0.1977890530862495 2.596551989891461 >50 0.005708020317987487 0.308852338671555 >100 0.0013314364171584272 0.16348359263705206 >500 7.004690934873565E-5 0.03447566948715216 >1k 1.400938186974713E-4 0.1342230087915234 >5k 0.0 0.0 >10k+ 7.004690934873565E-5 4.405866017809652 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 77828 4.413276574896356 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1341102366491125E-4 0.0 12-13 0.0 2.835275591622781E-5 0.0 1.1341102366491125E-4 0.0 14-15 0.0 5.670551183245562E-5 0.0 1.1341102366491125E-4 0.0 16-17 0.0 5.670551183245562E-5 0.0 1.1341102366491125E-4 0.0 18-19 0.0 5.670551183245562E-5 0.0 1.7011653549736688E-4 0.0 20-21 0.0 5.670551183245562E-5 0.0 1.7011653549736688E-4 0.0 22-23 0.0 5.670551183245562E-5 0.0 2.268220473298225E-4 2.835275591622781E-5 24-25 0.0 5.670551183245562E-5 0.0 2.268220473298225E-4 5.670551183245562E-5 26-27 0.0 5.670551183245562E-5 0.0 2.835275591622781E-4 5.670551183245562E-5 28-29 0.0 5.670551183245562E-5 0.0 4.5364409465964504E-4 5.670551183245562E-5 30-31 0.0 5.670551183245562E-5 0.0 0.001219168504397796 5.670551183245562E-5 32-33 0.0 5.670551183245562E-5 0.0 0.0031188031507850596 5.670551183245562E-5 34-35 0.0 5.670551183245562E-5 0.0 0.006549486616648625 5.670551183245562E-5 36-37 0.0 5.670551183245562E-5 0.0 0.011624629925653402 5.670551183245562E-5 38-39 0.0 5.670551183245562E-5 0.0 0.019620107094029646 5.670551183245562E-5 40-41 0.0 5.670551183245562E-5 0.0 0.028097581112981763 1.1341102366491125E-4 42-43 0.0 5.670551183245562E-5 0.0 0.036575055131933876 1.1341102366491125E-4 44-45 2.835275591622781E-5 5.670551183245562E-5 0.0 0.0484265071049171 1.1341102366491125E-4 46-47 5.670551183245562E-5 5.670551183245562E-5 0.0 0.06325499844910426 1.1341102366491125E-4 48-49 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.07856548664386728 1.1341102366491125E-4 50-51 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.09543537641402282 1.1341102366491125E-4 52-53 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.1157926551618744 1.1341102366491125E-4 54-55 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.13807792131202945 1.1341102366491125E-4 56-57 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.1637088126602994 1.1341102366491125E-4 58-59 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.1932523843250088 1.1341102366491125E-4 60-61 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.2271055748889848 1.1341102366491125E-4 62-63 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.2591725418302384 1.1341102366491125E-4 64-65 5.670551183245562E-5 1.1341102366491125E-4 0.0 0.29716523475798373 1.1341102366491125E-4 66-67 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.33807826154510046 1.1341102366491125E-4 68-69 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.38469019227137896 1.1341102366491125E-4 70-71 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.4336837544946206 1.1341102366491125E-4 72-73 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.4873838741999561 1.7011653549736688E-4 74-75 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.5407437608342969 1.7011653549736688E-4 76-77 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.6001711372347103 1.7011653549736688E-4 78-79 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.6715917293876882 1.7011653549736688E-4 80-81 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.748484403432498 1.7011653549736688E-4 82-83 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.8311326869283021 1.7011653549736688E-4 84-85 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.9107188727851536 1.7011653549736688E-4 86-87 5.670551183245562E-5 1.7011653549736688E-4 0.0 0.9973081893533133 1.7011653549736688E-4 88-89 5.670551183245562E-5 1.7011653549736688E-4 0.0 1.09594742718587 1.7011653549736688E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1230 0.0 51.206657 1 GTATCAA 2380 0.0 44.321728 1 TCAACGC 2805 0.0 36.24552 4 ATCAACG 2860 0.0 35.38449 3 TATCAAC 2910 0.0 35.189034 2 CAACGCA 2975 0.0 33.997974 5 AACGCAG 3190 0.0 30.959835 6 ACGCAGA 3750 0.0 25.835627 7 GTACATG 2610 0.0 25.309038 1 CGCAGAG 3875 0.0 24.640362 8 GTGGTAT 775 0.0 24.447048 1 ACATGGG 2680 0.0 23.483246 3 TACATGG 2685 0.0 23.264078 2 GTATAGG 515 0.0 22.868992 1 GCAGAGT 4235 0.0 20.886139 9 GAGTACA 2120 0.0 20.530972 1 TTCGCGC 65 0.005773844 19.144972 16-17 AGAGTAC 3695 0.0 19.096838 10-11 CAGAGTA 4090 0.0 18.596876 10-11 GAGTACT 2790 0.0 18.497873 12-13 >>END_MODULE