FastQCFastQC Report
Sat 18 Jun 2016
SRR1931035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763497
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43740.24802990875516093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.2310182552054242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.13994920320250048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.056.1369251
GTATCAA43400.040.6377371
ATCAACG53400.032.0411383
CAACGCA53350.032.0102355
TCAACGC53650.031.8909244
TATCAAC56100.031.0126532
AACGCAG55650.030.770766
ACGCAGA64700.025.9534537
CGCAGAG65650.025.5056368
GTACCGT1201.7273561E-423.7173526
TAACCCG1652.4591245E-622.9999495
GTGGTAT13650.021.9408421
GCAGAGT75000.021.5037339
TGGTATC12700.021.3349592
GTACATG40000.021.2734721
GAGTACT54050.020.57990312-13
ACATGGG39350.020.4121463
AGAGTAC69900.020.05286610-11
TACATGG42300.019.5537262
CAGAGTA73800.019.28239810-11
Produced by FastQC (version 0.11.5)