##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931035_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1763497 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30321117642956 31.0 31.0 34.0 30.0 34.0 2 31.4682395263502 31.0 31.0 34.0 30.0 34.0 3 31.52814889960119 31.0 31.0 34.0 30.0 34.0 4 35.30946522732956 37.0 35.0 37.0 33.0 37.0 5 35.1863048250153 37.0 35.0 37.0 32.0 37.0 6 35.212951312080484 37.0 35.0 37.0 32.0 37.0 7 35.09190092186151 37.0 35.0 37.0 32.0 37.0 8 35.09244813005069 37.0 35.0 37.0 32.0 37.0 9 36.58798512274191 39.0 35.0 39.0 32.0 39.0 10-11 36.533105528390465 38.0 35.0 39.0 32.0 39.0 12-13 36.41930692255218 38.0 35.0 39.0 32.0 39.0 14-15 37.47735323621191 39.0 36.0 41.0 32.0 41.0 16-17 37.42792616035071 39.0 36.0 41.0 32.0 41.0 18-19 37.45456045573086 39.0 36.0 41.0 32.0 41.0 20-21 37.43968489881185 39.0 36.0 41.0 32.0 41.0 22-23 37.33977063754574 39.0 36.0 40.5 32.0 41.0 24-25 37.21969813387831 39.0 36.0 40.5 31.0 41.0 26-27 36.96634953164082 39.0 36.0 40.0 31.0 41.0 28-29 36.77986466662546 39.0 36.0 40.0 30.0 41.0 30-31 36.55439221047725 38.0 35.0 40.0 30.0 41.0 32-33 36.392731884431896 38.0 35.0 40.0 30.0 41.0 34-35 36.47481424691962 38.0 35.0 40.0 30.0 41.0 36-37 36.54645712467897 38.0 35.0 40.0 30.0 41.0 38-39 36.53545086835986 39.0 35.0 40.0 30.0 41.0 40-41 36.42209768431701 39.0 35.0 40.0 30.0 41.0 42-43 36.28727409232905 38.0 35.0 40.0 30.0 41.0 44-45 36.130383266883925 38.0 35.0 40.0 30.0 41.0 46-47 35.89563123725189 38.0 35.0 40.0 29.0 41.0 48-49 35.68922600945734 38.0 35.0 40.0 28.0 41.0 50-51 35.43750967537795 38.0 34.0 40.0 27.5 41.0 52-53 35.23712232002663 38.0 34.0 40.0 27.0 41.0 54-55 35.08926865200225 38.0 34.0 40.0 26.0 41.0 56-57 34.93553462240084 38.0 34.0 40.0 26.0 41.0 58-59 34.735249620498365 38.0 33.5 40.0 26.0 41.0 60-61 34.43679717062178 37.5 33.0 40.0 26.0 41.0 62-63 34.183022993517994 37.0 33.0 40.0 25.0 41.0 64-65 33.87779508556011 37.0 32.5 40.0 24.5 41.0 66-67 33.56105170578685 36.0 32.0 39.0 23.5 41.0 68-69 33.22508203869924 36.0 32.0 39.0 23.0 40.0 70-71 32.85066490047899 35.5 31.0 39.0 22.0 40.0 72-73 32.42271662497866 35.0 31.0 38.0 22.0 40.0 74-75 31.901068445253948 35.0 31.0 37.5 21.0 39.5 76-77 30.518853448574056 33.5 29.0 36.0 20.0 39.0 78-79 31.263918226115496 34.0 30.0 36.0 20.0 39.0 80-81 31.22229411221 34.0 30.0 36.0 20.0 38.5 82-83 30.94298175726979 34.0 30.0 36.0 20.0 37.5 84-85 30.58093804525894 34.0 30.0 35.0 19.0 37.0 86-87 30.21685917242842 34.0 30.0 35.0 18.0 36.5 88-89 29.84776157827317 34.0 29.5 35.0 15.0 36.0 90-91 29.494211784879703 34.0 29.0 35.0 9.0 36.0 92-93 29.156271601255916 34.0 29.0 35.0 4.5 35.5 94-95 28.755177638521644 34.0 29.0 35.0 2.0 35.0 96-97 28.304451893028453 34.0 29.0 35.0 2.0 35.0 98-99 27.79206372338598 33.0 28.5 35.0 2.0 35.0 100-101 26.63649952339017 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24909922490651937 1101 2 0.3291901401092403 1101 3 0.2951616584138286 1101 4 0.19381302441851744 1101 5 0.2988711597145155 1101 6 0.3368887654564787 1101 7 0.28464115990603034 1101 8 0.3787354917526997 1101 9 0.5860516835432108 1101 10-11 0.4604859495777873 1101 12-13 0.40848739971437453 1101 14-15 0.4252897452822637 1101 16-17 0.5929576859502248 1101 18-19 0.6051813745218695 1101 20-21 0.4632447246561 1101 22-23 0.5138043397996128 1101 24-25 0.43156742952106697 1101 26-27 0.3795792303806991 1101 28-29 0.43285055387036664 1101 30-31 0.5821668337534831 1101 32-33 0.4820691493069873 1101 34-35 0.5134228893597381 1101 36-37 0.5902091531734612 1101 38-39 0.6655995875981162 1101 40-41 0.5444004488892631 1101 42-43 0.6229887853894382 1101 44-45 0.6123343746091692 1101 46-47 0.6858269896132825 1101 48-49 0.6733523673245898 1101 50-51 0.8079823223144018 1101 52-53 0.544591141063222 1101 54-55 0.5389708183897426 1101 56-57 0.7240679328836919 1101 58-59 0.6357191087229523 1101 60-61 0.6188559164220706 1101 62-63 0.4745317189342515 1101 64-65 0.7623106480120967 1101 66-67 0.6668198442484794 1101 68-69 0.7042688647091424 1101 70-71 0.5875676360321052 1101 72-73 0.49611312942690944 1101 74-75 0.602709313482297 1101 76-77 0.6674660650543167 1101 78-79 0.6206619131606139 1101 80-81 0.525808904857108 1101 82-83 0.6732264077050658 1101 84-85 0.6753253592596522 1101 86-87 0.6091181360271314 1101 88-89 0.6236889782153838 1101 90-91 0.4928809803444203 1101 92-93 0.5900651749403352 1101 94-95 0.7255772241737226 1101 96-97 0.8699249186872784 1101 98-99 1.0377158021965514 1101 100-101 1.141737895004944 1102 1 0.3728920650448657 1102 2 0.377316328553448 1102 3 0.2973716031652103 1102 4 0.19598254335127052 1102 5 0.3383057971548098 1102 6 0.3285056484761384 1102 7 0.3998951687997092 1102 8 0.29236498163876234 1102 9 0.4158679706563575 1102 10-11 0.3550232236798081 1102 12-13 0.33991997094616266 1102 14-15 0.4178828369590448 1102 16-17 0.4502088800091286 1102 18-19 0.40822229443383407 1102 20-21 0.49972977458946843 1102 22-23 0.5157822104070462 1102 24-25 0.5911603265550127 1102 26-27 0.45836765362404464 1102 28-29 0.36432654542586107 1102 30-31 0.4441598266115889 1102 32-33 0.5342543893914353 1102 34-35 0.5758628121015121 1102 36-37 0.5985016863750303 1102 38-39 0.41111157310471214 1102 40-41 0.536089200030915 1102 42-43 0.6109785741271025 1102 44-45 0.5697120865078347 1102 46-47 0.6165835513225417 1102 48-49 0.6602603911009552 1102 50-51 0.49384301793793384 1102 52-53 0.21595618620538204 1102 54-55 0.43281261868619936 1102 56-57 0.3969294819411644 1102 58-59 0.5027061874335246 1102 60-61 0.44012888053293153 1102 62-63 0.10113415346688015 1102 64-65 0.32311856548354 1102 66-67 0.22471111376890462 1102 68-69 0.2769185525319884 1102 70-71 0.27828691615308543 1102 72-73 0.3670544520998007 1102 74-75 0.3413466639441971 1102 76-77 0.25013836139054035 1102 78-79 0.4467680084757113 1102 80-81 0.441663312019962 1102 82-83 0.36443828391222866 1102 84-85 0.2800634149275041 1102 86-87 0.31522468051798924 1102 88-89 0.3909747588079924 1102 90-91 0.33972477198675577 1102 92-93 0.26488587894848337 1102 94-95 0.3048919316854395 1102 96-97 0.5315406039049009 1102 98-99 0.5027347424412056 1102 100-101 0.7299275669569099 1103 1 0.286805142786303 1103 2 0.42206286002522475 1103 3 0.3939790345060814 1103 4 0.2698362544247317 1103 5 0.290356267578737 1103 6 0.3956941814277002 1103 7 0.36940539378887394 1103 8 0.34232317104294907 1103 9 0.4074305486407823 1103 10-11 0.501362935865302 1103 12-13 0.4623711663789649 1103 14-15 0.5017527031213476 1103 16-17 0.5344460664843922 1103 18-19 0.582190273208937 1103 20-21 0.5991982765442785 1103 22-23 0.5535229496884355 1103 24-25 0.5088748849606901 1103 26-27 0.5972519455364349 1103 28-29 0.4931850504891031 1103 30-31 0.5726387527578751 1103 32-33 0.6160293476902865 1103 34-35 0.5262312269326728 1103 36-37 0.5889719351809077 1103 38-39 0.5832482040025226 1103 40-41 0.3943779864729038 1103 42-43 0.6183197266985658 1103 44-45 0.5696542959460871 1103 46-47 0.5563101844531744 1103 48-49 0.394242117024838 1103 50-51 0.3510534850345266 1103 52-53 0.5453899700424145 1103 54-55 0.7326471275320969 1103 56-57 0.5671126167288989 1103 58-59 0.6086214016745188 1103 60-61 0.45658474299139584 1103 62-63 0.4317565840853703 1103 64-65 0.5498090488665639 1103 66-67 0.6135163814661695 1103 68-69 0.40257362168365063 1103 70-71 0.6229507020478096 1103 72-73 0.5137325893173639 1103 74-75 0.597715665437125 1103 76-77 0.5402859462266463 1103 78-79 0.5730627140722042 1103 80-81 0.5677785166291969 1103 82-83 0.6261218280497687 1103 84-85 0.5783542152829995 1103 86-87 0.7055644627526441 1103 88-89 0.693543374154892 1103 90-91 0.7576945720401689 1103 92-93 0.5661029832035318 1103 94-95 0.6202887383190969 1103 96-97 0.6469155201742574 1103 98-99 0.5925679816459706 1103 100-101 0.9619912591608752 1104 1 0.3201716147634386 1104 2 0.3472480567168823 1104 3 0.3178844236780307 1104 4 0.24824176786659535 1104 5 0.3705733602150545 1104 6 0.3925977893975485 1104 7 0.34933752860792566 1104 8 0.3647409457830193 1104 9 0.5065444351133124 1104 10-11 0.39051676130749513 1104 12-13 0.49089844707707897 1104 14-15 0.5348322324145371 1104 16-17 0.4132639144056327 1104 18-19 0.6046067534707333 1104 20-21 0.5158778879936818 1104 22-23 0.5124116142010919 1104 24-25 0.38179379538969727 1104 26-27 0.5778485792025307 1104 28-29 0.6606823407145797 1104 30-31 0.5263270665532147 1104 32-33 0.6229569639171757 1104 34-35 0.51049252859098 1104 36-37 0.5479179882303526 1104 38-39 0.704971016506839 1104 40-41 0.6265780472270848 1104 42-43 0.6807826183213876 1104 44-45 0.8008865184444716 1104 46-47 0.4986817376890258 1104 48-49 0.4247551273944694 1104 50-51 0.29059228831525274 1104 52-53 0.46292825207989097 1104 54-55 0.6193289309647056 1104 56-57 0.6764127527354091 1104 58-59 0.7277543946463609 1104 60-61 0.6773787241852105 1104 62-63 0.6359488814218537 1104 64-65 0.47211403139973385 1104 66-67 0.3584053497497308 1104 68-69 0.2936217003327002 1104 70-71 0.5188539372079077 1104 72-73 0.4629320839530422 1104 74-75 0.4936163116406824 1104 76-77 0.5642057472567039 1104 78-79 0.6634164345753568 1104 80-81 0.4328843876922619 1104 82-83 0.5434603289403697 1104 84-85 0.57411177848806 1104 86-87 0.7398038445788622 1104 88-89 0.9343547358769939 1104 90-91 0.9922393644403762 1104 92-93 0.8266836171181708 1104 94-95 0.9533597240556695 1104 96-97 0.8639071871861184 1104 98-99 0.8648985948489596 1104 100-101 1.178375815862477 1105 1 0.23165537900474575 1105 2 0.2703644463395136 1105 3 0.2658419465575079 1105 4 0.22014436665524784 1105 5 0.1958649679598352 1105 6 0.17294811891540718 1105 7 0.18654525382293485 1105 8 0.2480739455962464 1105 9 0.4299826716368784 1105 10-11 0.3572331224697436 1105 12-13 0.30129588075104863 1105 14-15 0.3498316876650307 1105 16-17 0.28312992826227656 1105 18-19 0.33627065192053607 1105 20-21 0.24389391029950502 1105 22-23 0.26441690382994665 1105 24-25 0.36160721312585764 1105 26-27 0.2466275398649529 1105 28-29 0.2991534956724706 1105 30-31 0.09777902409388162 1105 32-33 0.35303775360384293 1105 34-35 0.3396171161377879 1105 36-37 0.16188403143677732 1105 38-39 0.22611091040297993 1105 40-41 0.1838023150392729 1105 42-43 0.06491995745352597 1105 44-45 0.10482033249144251 1105 46-47 0.04019053817285112 1105 48-49 0.2771010522234931 1105 50-51 0.18796231553049125 1105 52-53 0.11245754403893926 1105 54-55 0.09924240473223733 1105 56-57 0.1603190180217524 1105 58-59 0.2233838916091102 1105 60-61 0.09814602053783972 1105 62-63 0.05094211096373158 1105 64-65 0.004147247778682583 1105 66-67 0.10677304677117405 1105 68-69 0.052512234587439366 1105 70-71 0.11015173063889705 1105 72-73 0.189368888563628 1105 74-75 0.2820094784807772 1105 76-77 0.1110650440184493 1105 78-79 0.25262361281739487 1105 80-81 0.24011921697443483 1105 82-83 0.13468057894703733 1105 84-85 0.16589428780439164 1105 86-87 0.19858381691893 1105 88-89 0.19811938852692634 1105 90-91 0.19072996362035965 1105 92-93 0.3106382428196781 1105 94-95 0.3934617367383275 1105 96-97 0.4483739330082237 1105 98-99 0.3889498413247665 1105 100-101 0.4719521630613528 1106 1 0.20360856872337152 1106 2 0.27186320429161626 1106 3 0.2860667959808474 1106 4 0.1207503013299629 1106 5 0.23256406144902542 1106 6 0.24930671702564666 1106 7 0.29293370615813785 1106 8 0.23958602278736407 1106 9 0.3140860526787037 1106 10-11 0.34446399605900524 1106 12-13 0.21132381652186183 1106 14-15 0.33097600434079055 1106 16-17 0.33199114157183374 1106 18-19 0.39804487570243197 1106 20-21 0.27514025135668163 1106 22-23 0.3276354652311966 1106 24-25 0.3506868663528948 1106 26-27 0.29256338543873284 1106 28-29 0.3990127449027483 1106 30-31 0.45569784215022224 1106 32-33 0.44731534567452513 1106 34-35 0.49891436274379686 1106 36-37 0.334219070490235 1106 38-39 0.4317829382969691 1106 40-41 0.38493711640231254 1106 42-43 0.33180798844342974 1106 44-45 0.3769338148265433 1106 46-47 0.3338521360527622 1106 48-49 0.25113004781653814 1106 50-51 0.22197790942947648 1106 52-53 0.32083423498587393 1106 54-55 0.4455810981934931 1106 56-57 0.3671462411610591 1106 58-59 0.24398363665809342 1106 60-61 0.38346440422098027 1106 62-63 0.1977339498927435 1106 64-65 0.2931961727572343 1106 66-67 0.34808434591644755 1106 68-69 0.15429089994801615 1106 70-71 0.34995231584986897 1106 72-73 0.4560799160469955 1106 74-75 0.5773788578636037 1106 76-77 0.47661287811668984 1106 78-79 0.4692809895484693 1106 80-81 0.4130229210201719 1106 82-83 0.33685527591099174 1106 84-85 0.44261178526065237 1106 86-87 0.5529129543896367 1106 88-89 0.6986766472373418 1106 90-91 0.6047430893648595 1106 92-93 0.6990859055763252 1106 94-95 0.6898721111227353 1106 96-97 0.6419499425466242 1106 98-99 0.6526456250682564 1106 100-101 0.7873861542210747 1107 1 0.15594173537071754 1107 2 0.17971180152045463 1107 3 0.1599450148042898 1107 4 -0.07185436886728525 1107 5 0.10333588525600845 1107 6 0.152563975551665 1107 7 0.0794935696956216 1107 8 0.2059450677866579 1107 9 0.11729561814158984 1107 10-11 0.13830671161345975 1107 12-13 0.130851291364543 1107 14-15 0.1491026964080291 1107 16-17 0.12452800921217033 1107 18-19 0.2754423969605355 1107 20-21 0.2305521730430371 1107 22-23 0.12622746221674674 1107 24-25 0.16659573227051538 1107 26-27 0.20438240901986404 1107 28-29 0.1666101737079515 1107 30-31 0.19849290384518525 1107 32-33 0.18650437745458248 1107 34-35 0.21607264025424655 1107 36-37 0.31911438689606086 1107 38-39 0.35916355122193266 1107 40-41 0.12176208398879851 1107 42-43 0.2296700925959385 1107 44-45 0.18881669239640075 1107 46-47 0.19978456646136777 1107 48-49 0.17325709074227547 1107 50-51 0.13165737312366588 1107 52-53 0.15799387193195003 1107 54-55 0.24497648778885406 1107 56-57 0.48790840507796673 1107 58-59 0.3431211847495419 1107 60-61 0.3604723146132969 1107 62-63 0.09014660685890163 1107 64-65 0.07231763889445375 1107 66-67 0.2041700343591799 1107 68-69 0.2712040559849953 1107 70-71 0.3024521684836685 1107 72-73 0.355427668987339 1107 74-75 0.48435087295051815 1107 76-77 0.47876061718063667 1107 78-79 0.5134533400380512 1107 80-81 0.5844594580807296 1107 82-83 0.4893046996879704 1107 84-85 0.5425624563621234 1107 86-87 0.2999242408077727 1107 88-89 0.37443968689026974 1107 90-91 0.4738570786061409 1107 92-93 0.5077264445671652 1107 94-95 0.5768874984109935 1107 96-97 0.5509850845216242 1107 98-99 0.4855150016372818 1107 100-101 0.8290725989838492 1108 1 0.10773490073436776 1108 2 0.11606712688945464 1108 3 0.07115079637115329 1108 4 0.0553114232642784 1108 5 0.02639635003486518 1108 6 0.19514556009818307 1108 7 0.07650694447160333 1108 8 0.12636719108760985 1108 9 0.14096247637849046 1108 10-11 0.10910854638017042 1108 12-13 0.13159915206919237 1108 14-15 0.1597488718614315 1108 16-17 0.12630626837155035 1108 18-19 0.11586042814001019 1108 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-1.5477156966449357 2316 82-83 -1.0034353226588912 2316 84-85 -1.0545874146441214 2316 86-87 -1.1020430613788683 2316 88-89 -1.364587980913658 2316 90-91 -1.1082532843770174 2316 92-93 -0.9552846651722753 2316 94-95 -1.4059230321343001 2316 96-97 -1.2609142256507546 2316 98-99 -1.5821326099142041 2316 100-101 -1.7767850384607478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 16.0 5 34.0 6 95.0 7 238.0 8 545.0 9 1066.0 10 1874.0 11 2944.0 12 4252.0 13 5641.0 14 7107.0 15 9008.0 16 10615.0 17 12369.0 18 10905.0 19 9015.0 20 8659.0 21 9372.0 22 10130.0 23 12112.0 24 14659.0 25 18743.0 26 24235.0 27 30733.0 28 39215.0 29 49637.0 30 60714.0 31 73264.0 32 88782.0 33 106637.0 34 132045.0 35 164079.0 36 214652.0 37 292005.0 38 284554.0 39 53520.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.03671111257292 22.662251753272198 15.627160223588636 22.67387691056625 2 14.818580743506482 22.257842068670627 40.93862107527955 21.98495611254334 3 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19.927450968161555 22-23 20.229771645997427 28.420716873926132 30.85716391927371 20.492347560802727 24-25 20.260654033741105 28.604151887708838 30.832294198936538 20.302899879613516 26-27 20.268235713082714 28.743473726770663 30.771544853296014 20.21674570685061 28-29 20.199280575743543 28.801353676665098 30.779444324575056 20.219921423016302 30-31 20.469155160607524 28.63964568095691 30.7364964776502 20.154702680785366 32-33 20.31172101649637 28.810939728832206 30.514827450279757 20.36251180439166 34-35 20.44763993710469 28.747864380922135 30.704112723823513 20.100382958149666 36-37 20.42494422245579 28.628219737297623 30.725721918356246 20.221114121890345 38-39 20.40353944764815 28.82383312007317 30.683520315192187 20.089107117086492 40-41 20.47084221503558 28.733136107089653 30.66593061242775 20.13009106544702 42-43 20.329458282067854 28.79174338175517 30.482844979706954 20.395953356470027 44-45 20.36297657120805 29.023863188807532 30.30612785955484 20.307032380429572 46-47 20.56471324821273 28.92405838591461 30.043747469236692 20.46748089663597 48-49 20.58764899286706 29.054626346376605 29.975344489514672 20.382380171241664 50-51 20.620933709956347 29.153779902783995 29.76209007592302 20.463196311336638 52-53 20.683374518255622 29.217338880667043 29.650844957926665 20.448441643150673 54-55 20.552957180358494 29.191154931063263 29.770309178132965 20.485578710445278 56-57 20.565163603348257 29.29792490552866 29.60129366260143 20.535617828521655 58-59 20.563816437792887 29.264234011329076 29.746095439302955 20.42585411157508 60-61 20.621552160206285 29.367689529353726 29.69483867927688 20.31591963116311 62-63 20.582006206755253 29.285103531644307 29.694842584785917 20.438047676814524 64-65 20.429020601036928 29.213732205844405 29.71173553192916 20.645511661189502 66-67 20.428160822352538 29.2164169241871 29.704324950667772 20.65109730279259 68-69 20.400345472325977 29.250047105987193 29.735999006440235 20.613608415246595 70-71 20.552712169619188 29.08288892305183 29.716264151769085 20.648134755559898 72-73 20.544345764203918 29.223237421818983 29.591832967719494 20.640583846257606 74-75 20.502191854585607 29.33210058393542 29.458758761234694 20.706948800244277 76-77 20.400798983937925 29.251506618894325 29.555273947497778 20.79242044966998 78-79 20.30422179621064 29.278951924551905 29.60936766768677 20.807458611550686 80-81 20.414965618001293 29.39446117100951 29.37946255037226 20.81111066061694 82-83 20.497653242392815 29.350574455187616 29.501552880441533 20.650219421978036 84-85 20.512056442398258 29.311192477219976 29.39355723315662 20.783193847225146 86-87 20.420760568348005 29.361546971727197 29.477367979644992 20.740324480279806 88-89 20.529227321532716 29.200272980566155 29.40807050542516 20.862429192475965 90-91 20.45084841014718 29.286262540855333 29.489195930670896 20.773693118326584 92-93 20.462614680980426 29.312957703022768 29.397068431989982 20.827359184006824 94-95 20.34176266242346 29.221195318711207 29.4280913620264 21.00895065683894 96-97 20.42904536527291 29.08520090626775 29.410974164110904 21.074779564348443 98-99 20.241514445445613 29.244478442549095 29.388708911894945 21.125298200110347 100-101 20.253621072221843 29.1223631228179 29.440991393804467 21.183024411155788 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 154.0 1 118.5 2 112.0 3 174.0 4 169.5 5 165.5 6 300.5 7 675.0 8 1182.5 9 1488.5 10 1329.0 11 1060.0 12 1057.5 13 1161.5 14 1356.5 15 1569.0 16 1742.5 17 1969.0 18 2231.0 19 2497.5 20 2951.0 21 3619.5 22 4385.5 23 5597.5 24 7429.5 25 9885.0 26 13252.0 27 17176.0 28 21487.0 29 27285.5 30 33600.0 31 39736.0 32 46382.5 33 52203.5 34 56534.5 35 59725.0 36 64619.5 37 69694.5 38 71475.0 39 72161.0 40 71617.5 41 70571.5 42 70497.5 43 71509.5 44 72729.0 45 73015.5 46 72006.0 47 69955.0 48 67547.0 49 64091.5 50 59173.0 51 53324.0 52 47678.0 53 42389.0 54 36668.0 55 30546.0 56 24413.0 57 18758.5 58 14419.5 59 10820.5 60 7682.5 61 5387.5 62 3729.0 63 2388.0 64 1350.5 65 744.0 66 401.5 67 187.5 68 95.5 69 54.0 70 38.0 71 24.0 72 13.5 73 9.0 74 6.5 75 6.5 76 5.5 77 2.5 78 1.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00453644094659645 2 0.006974777955392042 3 0.0017578708668061246 4 0.1645593953377862 5 0.002324925985130681 6 3.4023307099473375E-4 7 5.670551183245563E-5 8 5.103496064921007E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.9693858282718934E-4 24-25 5.670551183245562E-4 26-27 0.00272186456795787 28-29 1.9846929141359467E-4 30-31 0.006492781104816169 32-33 0.02166150551999805 34-35 0.014148025202197679 36-37 0.0010490519689004291 38-39 8.505826774868344E-5 40-41 0.002268220473298225 42-43 0.011823099217066998 44-45 0.0075134803178003706 46-47 0.011057574807328847 48-49 0.011312749610574897 50-51 0.18673125046427638 52-53 0.10944163783663935 54-55 0.018287527565966938 56-57 0.007456774805967915 58-59 0.018967993707956408 60-61 0.11726699846951823 62-63 0.2246955906361054 64-65 0.23084813866992684 66-67 0.2410834835556851 68-69 0.23626351504992638 70-71 0.22429865205327826 72-73 0.1564788599016613 74-75 0.2736891528593471 76-77 0.21332613551369806 78-79 0.006379370081151258 80-81 8.505826774868344E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 8.505826774868344E-5 90-91 0.006265959057486346 92-93 0.01956340158219719 94-95 0.0028919811034552366 96-97 2.8352755916227815E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1763497.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.63310033922964 #Duplication Level Percentage of deduplicated Percentage of total 1 77.47178146978428 49.29769643725655 2 14.00957869577096 17.829458537166552 3 3.981226535212876 7.600133627652138 4 1.6868797960313362 4.29365565284325 5 0.8228524097712755 2.6180324977676244 6 0.5125362422218979 1.9568562077278644 7 0.3048202081057745 1.3577658421473726 8 0.22412753396106447 1.1409543885862814 9 0.15476671302724454 0.886345720131187 >10 0.7712245344025773 8.737091177524734 >50 0.044038845936432015 1.891267684351711 >100 0.015541949877140776 1.6104158837659408 >500 3.571805126843853E-4 0.16006548521466252 >1k 2.6788538451328887E-4 0.6202608578641304 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4374 0.24802990875516093 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4074 0.2310182552054242 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2468 0.13994920320250048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.7011653549736688E-4 0.0 6 0.0 0.0 0.0 1.7011653549736688E-4 0.0 7 0.0 0.0 0.0 1.7011653549736688E-4 0.0 8 0.0 0.0 0.0 1.7011653549736688E-4 0.0 9 0.0 0.0 0.0 1.7011653549736688E-4 0.0 10-11 0.0 0.0 0.0 1.7011653549736688E-4 0.0 12-13 0.0 0.0 0.0 1.7011653549736688E-4 0.0 14-15 0.0 0.0 0.0 1.7011653549736688E-4 0.0 16-17 0.0 0.0 0.0 2.268220473298225E-4 0.0 18-19 0.0 0.0 0.0 2.835275591622781E-4 0.0 20-21 0.0 0.0 0.0 2.835275591622781E-4 0.0 22-23 0.0 0.0 0.0 3.1188031507850593E-4 2.835275591622781E-5 24-25 0.0 0.0 0.0 3.969385828271894E-4 5.670551183245562E-5 26-27 0.0 0.0 0.0 4.53644094659645E-4 5.670551183245562E-5 28-29 0.0 0.0 0.0 8.222299215706066E-4 5.670551183245562E-5 30-31 0.0 0.0 0.0 0.001984692914135947 5.670551183245562E-5 32-33 0.0 0.0 0.0 0.005472081891831968 5.670551183245562E-5 34-35 0.0 0.0 0.0 0.011341102366491124 5.670551183245562E-5 36-37 0.0 0.0 0.0 0.02188832756732787 5.670551183245562E-5 38-39 0.0 0.0 0.0 0.037425637809420716 5.670551183245562E-5 40-41 0.0 0.0 0.0 0.056847275612036764 5.670551183245562E-5 42-43 0.0 0.0 0.0 0.07618385514690412 5.670551183245562E-5 44-45 0.0 0.0 0.0 0.10028369767569778 5.670551183245562E-5 46-47 0.0 0.0 0.0 0.12994068036407208 5.670551183245562E-5 48-49 0.0 0.0 0.0 0.16146894494291741 5.670551183245562E-5 50-51 0.0 0.0 0.0 0.19526542999506097 5.670551183245562E-5 52-53 0.0 0.0 0.0 0.2295439118977804 5.670551183245562E-5 54-55 0.0 0.0 0.0 0.26816036545568267 5.670551183245562E-5 56-57 0.0 0.0 0.0 0.31321289460656865 5.670551183245562E-5 58-59 0.0 0.0 0.0 0.36334056706645945 5.670551183245562E-5 60-61 0.0 0.0 0.0 0.4211518363796479 5.670551183245562E-5 62-63 0.0 0.0 0.0 0.477914053723936 5.670551183245562E-5 64-65 0.0 0.0 0.0 0.543749152961417 5.670551183245562E-5 66-67 0.0 0.0 0.0 0.618912308895337 5.670551183245562E-5 68-69 0.0 0.0 0.0 0.7025245860922928 5.670551183245562E-5 70-71 0.0 0.0 0.0 0.7928281136854783 5.670551183245562E-5 72-73 0.0 0.0 0.0 0.8909853546674591 5.670551183245562E-5 74-75 0.0 0.0 0.0 0.9893694176967696 5.670551183245562E-5 76-77 0.0 0.0 0.0 1.0998317547463932 5.670551183245562E-5 78-79 0.0 0.0 0.0 1.2304529012524545 5.670551183245562E-5 80-81 0.0 0.0 0.0 1.3744565485509757 5.670551183245562E-5 82-83 0.0 0.0 0.0 1.5288373045148362 5.670551183245562E-5 84-85 0.0 0.0 0.0 1.681658658903304 5.670551183245562E-5 86-87 0.0 0.0 0.0 1.8508679062113518 5.670551183245562E-5 88-89 0.0 0.0 0.0 2.040944781873743 5.670551183245562E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2405 0.0 56.136925 1 GTATCAA 4340 0.0 40.637737 1 ATCAACG 5340 0.0 32.041138 3 CAACGCA 5335 0.0 32.010235 5 TCAACGC 5365 0.0 31.890924 4 TATCAAC 5610 0.0 31.012653 2 AACGCAG 5565 0.0 30.77076 6 ACGCAGA 6470 0.0 25.953453 7 CGCAGAG 6565 0.0 25.505636 8 GTACCGT 120 1.7273561E-4 23.717352 6 TAACCCG 165 2.4591245E-6 22.999949 5 GTGGTAT 1365 0.0 21.940842 1 GCAGAGT 7500 0.0 21.503733 9 TGGTATC 1270 0.0 21.334959 2 GTACATG 4000 0.0 21.273472 1 GAGTACT 5405 0.0 20.579903 12-13 ACATGGG 3935 0.0 20.412146 3 AGAGTAC 6990 0.0 20.052866 10-11 TACATGG 4230 0.0 19.553726 2 CAGAGTA 7380 0.0 19.282398 10-11 >>END_MODULE