FastQCFastQC Report
Sat 18 Jun 2016
SRR1931034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431408
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1096884.511295512723492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.056.6369861
GTATCAA38400.047.4033361
ATCAACG45150.039.7406923
TCAACGC46600.038.609894
CAACGCA46850.038.3990975
TATCAAC47500.037.8852772
AACGCAG51200.034.9145746
ACGCAGA56500.031.1349747
CGCAGAG57750.030.0229728
GCAGAGT62850.025.9396789
GTGGTAT10700.023.9606171
TACATGG37300.022.1296222
GTACATG37400.021.9361361
GAGTACT41600.021.76663412-13
CAGAGTA58650.021.47087110-11
AGAGTAC53300.021.26341210-11
ACATGGG36900.021.1713493
GTACTTT47000.019.34544614-15
TGGTATC11050.019.022982
AGTACTT47100.018.7149412-13
Produced by FastQC (version 0.11.5)