##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931034_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2431408 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.225767950093115 30.0 2.0 31.0 2.0 33.0 2 19.89770659634253 27.0 2.0 31.0 2.0 33.0 3 20.58156179464738 27.0 2.0 31.0 2.0 33.0 4 22.077894372314315 30.0 2.0 35.0 2.0 37.0 5 21.41610622322539 30.0 2.0 35.0 2.0 37.0 6 22.434626356415706 30.0 2.0 35.0 2.0 37.0 7 22.641303721958636 30.0 2.0 35.0 2.0 37.0 8 23.109137997407263 32.0 2.0 35.0 2.0 37.0 9 24.44757276442292 32.0 2.0 37.0 2.0 39.0 10-11 24.846214004395804 32.5 2.0 37.0 2.0 39.0 12-13 24.76176910662464 33.0 2.0 37.0 2.0 39.0 14-15 24.739747093042386 32.5 2.0 38.0 2.0 40.0 16-17 23.551366327658705 31.5 2.0 38.0 2.0 40.0 18-19 22.45186986305877 30.0 2.0 38.0 2.0 40.0 20-21 22.10177765311293 29.0 2.0 38.0 2.0 40.0 22-23 21.73006813336141 27.0 2.0 37.0 2.0 40.0 24-25 21.563430325144935 27.0 2.0 37.0 2.0 40.0 26-27 21.29325703460711 27.0 2.0 37.0 2.0 39.5 28-29 21.10100978527668 27.0 2.0 37.0 2.0 39.0 30-31 21.114176641682516 27.0 2.0 37.0 2.0 39.0 32-33 20.990681325388415 26.5 2.0 37.0 2.0 39.0 34-35 20.667725655258188 25.0 2.0 36.5 2.0 39.0 36-37 20.18375998598343 25.0 2.0 36.0 2.0 39.0 38-39 20.078140525983297 24.0 2.0 36.0 2.0 39.0 40-41 19.964036475984287 24.0 2.0 36.0 2.0 39.0 42-43 19.83229778794838 24.0 2.0 35.5 2.0 39.0 44-45 19.451384341912174 24.0 2.0 35.0 2.0 39.0 46-47 19.11866498752986 23.0 2.0 35.0 2.0 39.0 48-49 18.887125278850775 20.5 2.0 35.0 2.0 39.0 50-51 16.602351189105242 11.5 2.0 33.0 2.0 37.5 52-53 18.531202085376044 21.0 2.0 34.0 2.0 38.0 54-55 19.314765765350778 23.0 2.0 36.0 2.0 39.0 56-57 19.293429979666104 23.0 2.0 36.0 2.0 39.0 58-59 19.01252319643597 22.5 2.0 36.0 2.0 39.0 60-61 18.722220005856688 20.5 2.0 35.5 2.0 39.0 62-63 18.28568755223311 18.5 2.0 35.0 2.0 39.0 64-65 17.93967589972559 15.5 2.0 34.0 2.0 38.5 66-67 17.592478514506823 13.0 2.0 34.0 2.0 38.0 68-69 17.149328290439122 8.0 2.0 34.0 2.0 38.0 70-71 16.802524504320132 8.0 2.0 33.5 2.0 37.5 72-73 16.39766608483644 6.5 2.0 33.0 2.0 37.0 74-75 15.945303091871047 2.0 2.0 32.0 2.0 36.0 76-77 15.533223136552976 2.0 2.0 32.0 2.0 36.0 78-79 15.154057648901377 2.0 2.0 31.0 2.0 35.0 80-81 14.72035195244895 2.0 2.0 31.0 2.0 35.0 82-83 14.255986243361871 2.0 2.0 30.5 2.0 34.5 84-85 13.846204956140639 2.0 2.0 30.0 2.0 34.0 86-87 13.360501404947257 2.0 2.0 29.5 2.0 34.0 88-89 12.939322400847574 2.0 2.0 29.0 2.0 34.0 90-91 12.457867622381762 2.0 2.0 29.0 2.0 34.0 92-93 11.901954752143613 2.0 2.0 28.5 2.0 34.0 94-95 11.332369762705396 2.0 2.0 26.5 2.0 34.0 96-97 10.689720112790614 2.0 2.0 25.5 2.0 33.5 98-99 10.005381244118634 2.0 2.0 24.5 2.0 33.0 100-101 9.051114210366997 2.0 2.0 11.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.039341666590936 1101 2 9.671598852483324 1101 3 8.99019544270428 1101 4 11.098195847550205 1101 5 11.783677005745854 1101 6 10.898202094506317 1101 7 10.308603371955147 1101 8 9.792557087653254 1101 9 9.656761184393222 1101 10-11 9.186023359501334 1101 12-13 9.18364375399588 1101 14-15 9.746773667434361 1101 16-17 10.872594678367825 1101 18-19 11.850929353726684 1101 20-21 11.883430532302693 1101 22-23 11.850120765833577 1101 24-25 11.849251685742193 1101 26-27 11.995753549108901 1101 28-29 12.091784600380766 1101 30-31 11.344976207798961 1101 32-33 11.485724477819183 1101 34-35 11.202113241706066 1101 36-37 11.556553385095437 1101 38-39 11.343841463141558 1101 40-41 11.029615865120604 1101 42-43 10.633803898201823 1101 44-45 10.640361598911394 1101 46-47 10.452218401834099 1101 48-49 10.678492590543051 1101 50-51 12.415116829219425 1101 52-53 11.447566489620371 1101 54-55 11.134753409331907 1101 56-57 10.988171314473313 1101 58-59 10.754478242746817 1101 60-61 10.611376836043885 1101 62-63 10.582911984245225 1101 64-65 10.446829444520215 1101 66-67 10.267051360343999 1101 68-69 10.279820405876663 1101 70-71 10.166954305767387 1101 72-73 9.857587111275045 1101 74-75 9.581938650936914 1101 76-77 9.478260473828906 1101 78-79 9.086677121148275 1101 80-81 8.906940418386563 1101 82-83 8.505708455541685 1101 84-85 8.359849796688321 1101 86-87 8.08631438499751 1101 88-89 8.09368871549532 1101 90-91 8.026455736566176 1101 92-93 7.573066254918224 1101 94-95 7.330889922254334 1101 96-97 7.0942745060646235 1101 98-99 6.7239017351447 1101 100-101 6.3178174086657926 1102 1 7.679921480364094 1102 2 9.028018540834083 1102 3 8.443507642786905 1102 4 10.445735863016143 1102 5 11.167252126772109 1102 6 10.288570046117002 1102 7 10.171186263026218 1102 8 9.451349764637609 1102 9 9.277870203365477 1102 10-11 8.455294540421022 1102 12-13 8.666763636200521 1102 14-15 9.214215087546435 1102 16-17 10.13497603555684 1102 18-19 11.519911938656925 1102 20-21 11.480452478574446 1102 22-23 11.711114359651436 1102 24-25 11.383791838918537 1102 26-27 11.53004310907119 1102 28-29 11.56920903179569 1102 30-31 11.321022873088527 1102 32-33 11.04928378863157 1102 34-35 10.669117365073404 1102 36-37 11.222446092799803 1102 38-39 11.210404383555538 1102 40-41 10.81507828077298 1102 42-43 10.45998191803212 1102 44-45 10.441149262707324 1102 46-47 10.535515321358258 1102 48-49 10.041075481614119 1102 50-51 11.890036321597684 1102 52-53 10.869442648241574 1102 54-55 10.551807386514351 1102 56-57 10.422572499312459 1102 58-59 10.403256129103951 1102 60-61 10.47537026566857 1102 62-63 10.440636280068365 1102 64-65 10.157051661445884 1102 66-67 9.7670960500189 1102 68-69 9.72277883587211 1102 70-71 9.425050750720299 1102 72-73 9.30944507623304 1102 74-75 8.916321364043766 1102 76-77 8.909037822585804 1102 78-79 8.569613610168515 1102 80-81 8.134691249945552 1102 82-83 8.077287080238461 1102 84-85 7.846319749337129 1102 86-87 7.953750574762447 1102 88-89 7.802826131942178 1102 90-91 7.366109845103162 1102 92-93 7.242718191868047 1102 94-95 6.861846956607783 1102 96-97 6.549483203536051 1102 98-99 6.2043687058335735 1102 100-101 5.512336666936362 1103 1 7.893572155718356 1103 2 9.37720381684829 1103 3 8.775004905447794 1103 4 10.787722169450245 1103 5 11.470339067960051 1103 6 10.703680799243493 1103 7 10.166875125684225 1103 8 9.652652151785755 1103 9 9.59606305572714 1103 10-11 9.045062054712965 1103 12-13 8.845812833712273 1103 14-15 9.5504417602939 1103 16-17 10.541935786497262 1103 18-19 11.542318565850778 1103 20-21 11.784662965458967 1103 22-23 12.13531522479062 1103 24-25 11.936345102034831 1103 26-27 11.801843019953086 1103 28-29 11.6375298851048 1103 30-31 11.537540033558034 1103 32-33 11.405679403000129 1103 34-35 11.312699013156347 1103 36-37 11.521163253400179 1103 38-39 11.141946859586234 1103 40-41 11.213600847217332 1103 42-43 10.8854937091686 1103 44-45 10.740714337571116 1103 46-47 10.35811721338402 1103 48-49 10.498361033815932 1103 50-51 12.359988405894772 1103 52-53 11.42526169399737 1103 54-55 11.015998610278658 1103 56-57 10.831993712561871 1103 58-59 10.502062636819343 1103 60-61 10.554175337156074 1103 62-63 10.617591905534155 1103 64-65 10.261373742255461 1103 66-67 10.099524079952637 1103 68-69 9.788912467879772 1103 70-71 9.661483549977515 1103 72-73 9.51384222366854 1103 74-75 9.334127579933353 1103 76-77 9.099992335056777 1103 78-79 8.858219922766297 1103 80-81 8.29543623018188 1103 82-83 8.180537388258633 1103 84-85 7.857197948706498 1103 86-87 7.648892967433037 1103 88-89 7.096653656349874 1103 90-91 6.824784841455342 1103 92-93 6.574021612838543 1103 94-95 6.370598377295613 1103 96-97 6.037879610937955 1103 98-99 5.7858815903353324 1103 100-101 5.306051812237476 1104 1 7.84352052045292 1104 2 9.272888631746778 1104 3 8.708518407249212 1104 4 10.748840543734385 1104 5 11.55457385435638 1104 6 10.67939422144918 1104 7 10.395609951750966 1104 8 9.648110128390723 1104 9 9.305442065585478 1104 10-11 8.672512499356174 1104 12-13 8.532207857353612 1104 14-15 9.31219408053515 1104 16-17 10.49927025651806 1104 18-19 11.149552394103054 1104 20-21 11.321763485189184 1104 22-23 11.53641059023479 1104 24-25 11.325101694253892 1104 26-27 11.389812162425319 1104 28-29 11.257853675340403 1104 30-31 11.132110237285527 1104 32-33 11.147471451009931 1104 34-35 11.060177392780862 1104 36-37 10.972681945175086 1104 38-39 10.850693213970803 1104 40-41 10.386913157886699 1104 42-43 10.048358601298531 1104 44-45 10.309867914328901 1104 46-47 9.996823859118305 1104 48-49 10.055653077205484 1104 50-51 11.719235069517385 1104 52-53 10.834097091732303 1104 54-55 10.544261549604592 1104 56-57 10.482227799866735 1104 58-59 10.45329057154574 1104 60-61 10.259164473198194 1104 62-63 10.153674934913521 1104 64-65 9.920788503281845 1104 66-67 9.738134337733854 1104 68-69 9.360819633376135 1104 70-71 9.155341327242642 1104 72-73 8.977219103114134 1104 74-75 8.96958786347644 1104 76-77 8.54924766244284 1104 78-79 8.328928579742763 1104 80-81 8.175027602615687 1104 82-83 7.791312637361743 1104 84-85 7.334322458681294 1104 86-87 7.0814918989128355 1104 88-89 6.708487420428488 1104 90-91 6.649263653471428 1104 92-93 6.398209986443987 1104 94-95 6.0740147965134295 1104 96-97 5.746625248387334 1104 98-99 5.2979576466437415 1104 100-101 4.658313137344004 1105 1 7.885163872073825 1105 2 9.076722038738811 1105 3 8.61993761896943 1105 4 10.405374927952256 1105 5 10.47990475955595 1105 6 10.497081130016525 1105 7 10.107676716853835 1105 8 9.547294032877598 1105 9 9.387508576481604 1105 10-11 8.66279682499929 1105 12-13 8.505487819522756 1105 14-15 9.274679367937303 1105 16-17 10.129189118627437 1105 18-19 10.775770710525514 1105 20-21 11.298876226490332 1105 22-23 11.743053487040026 1105 24-25 11.586331966049901 1105 26-27 11.61520127484258 1105 28-29 11.53444216884083 1105 30-31 11.086884025446782 1105 32-33 11.231184409948803 1105 34-35 11.099572239857132 1105 36-37 11.125608354774421 1105 38-39 10.99044352169878 1105 40-41 10.787410591470596 1105 42-43 10.504961223180707 1105 44-45 10.219637309001403 1105 46-47 10.250353963781034 1105 48-49 10.131378508200761 1105 50-51 11.738730888735354 1105 52-53 10.473256368792843 1105 54-55 10.409410157945207 1105 56-57 10.130840581442811 1105 58-59 10.157150210835564 1105 60-61 9.859724064923796 1105 62-63 9.867436615789732 1105 64-65 9.886980155561329 1105 66-67 9.746130288189875 1105 68-69 9.353211195060013 1105 70-71 9.269344320756694 1105 72-73 9.173059055776555 1105 74-75 9.03399711304896 1105 76-77 8.62526175590945 1105 78-79 8.542846230235446 1105 80-81 8.297607623374882 1105 82-83 7.944137440833904 1105 84-85 7.707917545324447 1105 86-87 7.358790027369318 1105 88-89 7.11895060042003 1105 90-91 6.891528647764028 1105 92-93 6.699908056831077 1105 94-95 6.3422859824246185 1105 96-97 5.950341704965913 1105 98-99 5.572945786214877 1105 100-101 4.995242424073584 1106 1 7.649308734791141 1106 2 9.000915275028689 1106 3 8.51771769340942 1106 4 10.121637226930083 1106 5 10.991278101203964 1106 6 10.192785109612352 1106 7 9.39410112356897 1106 8 8.958880793507877 1106 9 8.767155968994299 1106 10-11 8.43349510658814 1106 12-13 8.135037526571118 1106 14-15 8.84990877351433 1106 16-17 10.091757664940523 1106 18-19 11.187356423590494 1106 20-21 11.268221772040643 1106 22-23 11.357692448896831 1106 24-25 10.998172611613509 1106 26-27 11.184113786827847 1106 28-29 11.503105599727927 1106 30-31 11.154233046220153 1106 32-33 11.098582845873949 1106 34-35 11.175350719304127 1106 36-37 11.25581802796248 1106 38-39 11.150642595022514 1106 40-41 10.815444636145664 1106 42-43 10.560598463887718 1106 44-45 10.0479468932811 1106 46-47 9.966702312765744 1106 48-49 10.270112594374382 1106 50-51 11.756896419317453 1106 52-53 10.809986507441103 1106 54-55 10.340006711964183 1106 56-57 10.338823223585074 1106 58-59 9.947819052846405 1106 60-61 9.801141369894445 1106 62-63 9.95487913625579 1106 64-65 9.91313531704277 1106 66-67 9.667041577217482 1106 68-69 9.407126687368564 1106 70-71 9.011051756420098 1106 72-73 8.83684648921313 1106 74-75 8.660943620324078 1106 76-77 8.387303706110893 1106 78-79 8.073008628297993 1106 80-81 7.8093779922720135 1106 82-83 7.66990295291899 1106 84-85 7.257519238549138 1106 86-87 7.14012685837354 1106 88-89 6.907117482361366 1106 90-91 6.6320500150283 1106 92-93 6.379789011510255 1106 94-95 6.040411409213943 1106 96-97 5.40744428001611 1106 98-99 5.031892674803764 1106 100-101 4.394478838760392 1107 1 7.393216905710972 1107 2 8.343879858381616 1107 3 8.209789835339382 1107 4 9.958338024339618 1107 5 10.895886574532717 1107 6 9.83835732326449 1107 7 9.657810788661667 1107 8 8.197100866546922 1107 9 5.660684247520912 1107 10-11 7.67327153621104 1107 12-13 7.601628071706706 1107 14-15 8.062200397069304 1107 16-17 9.288373362809082 1107 18-19 10.652732468265185 1107 20-21 10.937264921749527 1107 22-23 10.558201279543123 1107 24-25 10.549578234044443 1107 26-27 10.769234638699206 1107 28-29 10.680806997957685 1107 30-31 10.456855741116353 1107 32-33 10.918272700360234 1107 34-35 11.087358040845235 1107 36-37 11.374245528009514 1107 38-39 11.013109805641161 1107 40-41 10.491691676136071 1107 42-43 10.220223374528938 1107 44-45 9.748717847915056 1107 46-47 9.788299124773683 1107 48-49 9.91565732432274 1107 50-51 11.585883408208748 1107 52-53 10.532008243964388 1107 54-55 10.182102913157827 1107 56-57 9.951928438257788 1107 58-59 10.094462102144231 1107 60-61 9.823935868803328 1107 62-63 9.877004722966731 1107 64-65 9.782001013147667 1107 66-67 9.800386188177573 1107 68-69 9.643482130729208 1107 70-71 9.385415369054488 1107 72-73 8.986105413921305 1107 74-75 8.885466667834732 1107 76-77 8.813662444576552 1107 78-79 8.408936207709727 1107 80-81 8.110059191302236 1107 82-83 7.857527511416071 1107 84-85 7.535554453551066 1107 86-87 7.231141421503166 1107 88-89 7.253113283086579 1107 90-91 7.1214038275912035 1107 92-93 6.856438445257776 1107 94-95 6.563792839518799 1107 96-97 6.076593322818967 1107 98-99 5.618899393760994 1107 100-101 5.098361257302809 1108 1 6.762463205282746 1108 2 8.192719692676906 1108 3 7.91365375732784 1108 4 9.275855130092737 1108 5 11.085549230462707 1108 6 9.569225643875175 1108 7 8.986828313331188 1108 8 6.256275717598868 1108 9 6.940545423279616 1108 10-11 6.208934413235802 1108 12-13 5.111896760032902 1108 14-15 7.102327352012519 1108 16-17 8.554461789079795 1108 18-19 8.224945219615023 1108 20-21 8.272541755672446 1108 22-23 9.244058487232568 1108 24-25 9.017040938848016 1108 26-27 7.638726469829354 1108 28-29 9.38000523850879 1108 30-31 9.787226307584422 1108 32-33 10.076844195426325 1108 34-35 9.57336099653319 1108 36-37 9.636110462379275 1108 38-39 9.06262950634866 1108 40-41 9.579532918455932 1108 42-43 8.747721163581257 1108 44-45 8.068246334230455 1108 46-47 8.909111706229648 1108 48-49 9.506908371613747 1108 50-51 10.900907379536246 1108 52-53 10.35345850927811 1108 54-55 9.665435567691436 1108 56-57 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-14.909109332685428 2313 70-71 -14.558944387043073 2313 72-73 -14.158140012907964 2313 74-75 -13.705234551677465 2313 76-77 -13.313814689569787 2313 78-79 -12.943960133753686 2313 80-81 -12.51360696723435 2313 82-83 -12.061489256876614 2313 84-85 -11.659758046448934 2313 86-87 -11.172059798009029 2313 88-89 -10.773754094962198 2313 90-91 -10.285227269969772 2313 92-93 -9.74188472547393 2313 94-95 -9.174012038529982 2313 96-97 -8.53297222596152 2313 98-99 -7.861474081848762 2313 100-101 -6.928506120745972 2314 1 -18.963109313801223 2314 2 -17.646211605033017 2314 3 -18.321079067099642 2314 4 -19.765366243953064 2314 5 -19.083153059613625 2314 6 -20.13039267673551 2314 7 -20.34866786987492 2314 8 -20.812426572477467 2314 9 -22.148385874430307 2314 10-11 -22.560912915008473 2314 12-13 -22.49936278195183 2314 14-15 -22.470955090735572 2314 16-17 -21.249545844507992 2314 18-19 -20.14661966588116 2314 20-21 -19.79406129776267 2314 22-23 -19.42826975704224 2314 24-25 -19.26349341229702 2314 26-27 -18.997914751544695 2314 28-29 -18.794040563017926 2314 30-31 -18.81086529546901 2314 32-33 -18.69605656793245 2314 34-35 -18.36957793476452 2314 36-37 -17.89728648418561 2314 38-39 -17.797294157773475 2314 40-41 -17.669322043376127 2314 42-43 -17.540828454739355 2314 44-45 -17.167440688670307 2314 46-47 -16.843941728884854 2314 48-49 -16.603778651287016 2314 50-51 -14.37981781130345 2314 52-53 -16.267915536523418 2314 54-55 -17.025785806354367 2314 56-57 -17.00672247637636 2314 58-59 -16.736330580782596 2314 60-61 -16.44683394826984 2314 62-63 -16.02390991117961 2314 64-65 -15.686748986852333 2314 66-67 -15.352433933773646 2314 68-69 -14.909109332685428 2314 70-71 -14.558944387043073 2314 72-73 -14.158140012907964 2314 74-75 -13.705234551677465 2314 76-77 -13.313814689569787 2314 78-79 -12.943960133753686 2314 80-81 -12.51360696723435 2314 82-83 -12.061489256876614 2314 84-85 -11.659758046448934 2314 86-87 -11.172059798009029 2314 88-89 -10.773754094962198 2314 90-91 -10.285227269969772 2314 92-93 -9.74188472547393 2314 94-95 -9.174012038529982 2314 96-97 -8.53297222596152 2314 98-99 -7.861474081848762 2314 100-101 -6.928506120745972 2315 1 -18.963109313801223 2315 2 -17.646211605033017 2315 3 -18.321079067099642 2315 4 -19.765366243953064 2315 5 -19.083153059613625 2315 6 -20.13039267673551 2315 7 -20.34866786987492 2315 8 -20.812426572477467 2315 9 -22.148385874430307 2315 10-11 -22.560912915008473 2315 12-13 -22.49936278195183 2315 14-15 -22.470955090735572 2315 16-17 -21.249545844507992 2315 18-19 -20.14661966588116 2315 20-21 -19.79406129776267 2315 22-23 -19.42826975704224 2315 24-25 -19.26349341229702 2315 26-27 -18.997914751544695 2315 28-29 -18.794040563017926 2315 30-31 -18.81086529546901 2315 32-33 -18.69605656793245 2315 34-35 -18.36957793476452 2315 36-37 -17.89728648418561 2315 38-39 -17.797294157773475 2315 40-41 -17.669322043376127 2315 42-43 -17.540828454739355 2315 44-45 -17.167440688670307 2315 46-47 -16.843941728884854 2315 48-49 -16.603778651287016 2315 50-51 -14.37981781130345 2315 52-53 -16.267915536523418 2315 54-55 -17.025785806354367 2315 56-57 -17.00672247637636 2315 58-59 -16.736330580782596 2315 60-61 -16.44683394826984 2315 62-63 -16.02390991117961 2315 64-65 -15.686748986852333 2315 66-67 -15.352433933773646 2315 68-69 -14.909109332685428 2315 70-71 -14.558944387043073 2315 72-73 -14.158140012907964 2315 74-75 -13.705234551677465 2315 76-77 -13.313814689569787 2315 78-79 -12.943960133753686 2315 80-81 -12.51360696723435 2315 82-83 -12.061489256876614 2315 84-85 -11.659758046448934 2315 86-87 -11.172059798009029 2315 88-89 -10.773754094962198 2315 90-91 -10.285227269969772 2315 92-93 -9.74188472547393 2315 94-95 -9.174012038529982 2315 96-97 -8.53297222596152 2315 98-99 -7.861474081848762 2315 100-101 -6.928506120745972 2316 1 -18.963109313801223 2316 2 -17.646211605033017 2316 3 -18.321079067099642 2316 4 -19.765366243953064 2316 5 -19.083153059613625 2316 6 -20.13039267673551 2316 7 -20.34866786987492 2316 8 -20.812426572477467 2316 9 -22.148385874430307 2316 10-11 -22.560912915008473 2316 12-13 -22.49936278195183 2316 14-15 -22.470955090735572 2316 16-17 -21.249545844507992 2316 18-19 -20.14661966588116 2316 20-21 -19.79406129776267 2316 22-23 -19.42826975704224 2316 24-25 -19.26349341229702 2316 26-27 -18.997914751544695 2316 28-29 -18.794040563017926 2316 30-31 -18.81086529546901 2316 32-33 -18.69605656793245 2316 34-35 -18.36957793476452 2316 36-37 -17.89728648418561 2316 38-39 -17.797294157773475 2316 40-41 -17.669322043376127 2316 42-43 -17.540828454739355 2316 44-45 -17.167440688670307 2316 46-47 -16.843941728884854 2316 48-49 -16.603778651287016 2316 50-51 -14.37981781130345 2316 52-53 -16.267915536523418 2316 54-55 -17.025785806354367 2316 56-57 -17.00672247637636 2316 58-59 -16.736330580782596 2316 60-61 -16.44683394826984 2316 62-63 -16.02390991117961 2316 64-65 -15.686748986852333 2316 66-67 -15.352433933773646 2316 68-69 -14.909109332685428 2316 70-71 -14.558944387043073 2316 72-73 -14.158140012907964 2316 74-75 -13.705234551677465 2316 76-77 -13.313814689569787 2316 78-79 -12.943960133753686 2316 80-81 -12.51360696723435 2316 82-83 -12.061489256876614 2316 84-85 -11.659758046448934 2316 86-87 -11.172059798009029 2316 88-89 -10.773754094962198 2316 90-91 -10.285227269969772 2316 92-93 -9.74188472547393 2316 94-95 -9.174012038529982 2316 96-97 -8.53297222596152 2316 98-99 -7.861474081848762 2316 100-101 -6.928506120745972 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 614587.0 3 24891.0 4 94819.0 5 50718.0 6 27581.0 7 28070.0 8 27363.0 9 26409.0 10 27503.0 11 29337.0 12 31265.0 13 31719.0 14 32591.0 15 33787.0 16 35131.0 17 35834.0 18 35040.0 19 35538.0 20 37825.0 21 40151.0 22 43154.0 23 45844.0 24 49138.0 25 52757.0 26 56799.0 27 61018.0 28 65683.0 29 71026.0 30 77108.0 31 84395.0 32 92447.0 33 99490.0 34 105220.0 35 102338.0 36 81631.0 37 37804.0 38 5343.0 39 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.467637512136896 21.241460425638422 14.488548233912882 22.8023538283118 2 14.519795056904996 21.138473000222106 42.305102649348285 22.036629293524612 3 18.432569271248468 26.6248639429546 30.590980382073894 24.351586403723037 4 11.842086825738981 17.71820285807035 40.614410391624304 29.825299924566373 5 12.154558700573535 38.47532771695816 36.49496873181727 12.875144850651033 6 28.717956339867985 39.64988883219028 18.573473967906384 13.058680860035352 7 25.413016735554795 33.41993864914157 23.753678419702602 17.413366195601036 8 23.710663749930415 37.69522511582068 21.133692221097405 17.4604189131515 9 24.751543273347266 17.285409289926005 22.123585254869116 35.83946218185761 10-11 23.285661756458488 27.932470088629536 29.717984080430792 19.06388407448119 12-13 23.89347079624811 25.330432972553375 29.991524888106802 20.78457134309171 14-15 21.171598802479735 27.19874961021562 27.63014948471468 23.999502102589965 16-17 20.549808443999567 30.320543915649466 28.337377486792775 20.79227015355819 18-19 20.276489361130917 29.165024059079048 30.490979293677324 20.06750728611271 20-21 21.015324455779087 28.44739173609103 30.571890795728024 19.965393012401854 22-23 20.6325302075797 28.323643427664607 30.288401203156056 20.755425161599636 24-25 20.5278481876927 28.511355127464533 30.557094075547486 20.40370260929528 26-27 20.534277307823135 28.697963131704153 30.28763998574675 20.480119574725965 28-29 20.48451641764734 28.70498125495096 30.418117423887566 20.392384903514134 30-31 20.80444530000003 28.523269042501553 30.298335201938762 20.37395045555965 32-33 20.55476072343217 28.69720112200815 30.219249322585195 20.528788831974484 34-35 20.667991730552373 28.720923625619555 30.293462678217086 20.317621965610986 36-37 20.792552505863704 28.56576211644776 30.13085811956862 20.51082725811991 38-39 20.6670905292364 28.74591870787082 30.246232615056318 20.340758147836464 40-41 20.857971201713084 28.719162922349284 30.125139616488116 20.297726259449515 42-43 20.67240091016281 28.719794933751853 29.96286648684519 20.644937669240147 44-45 20.788247779944864 28.896043389865923 29.812930717629882 20.502778112559334 46-47 20.832696682444226 28.859647999635797 29.506485302967576 20.801170014952405 48-49 20.790757072270548 28.931433576486164 29.51943996844139 20.7583693828019 50-51 21.08024317511954 29.148915921164193 29.0868532009021 20.683987702814164 52-53 20.556996539345562 29.35795572119372 29.221387151387624 20.8636605880731 54-55 20.624792041685385 29.267156913427307 29.259637516740728 20.84841352814658 56-57 20.56629925052853 29.39091563460352 29.304074833347972 20.738710281519978 58-59 20.490922626253838 29.311667031912048 29.451089539290145 20.74632080254397 60-61 20.529874230504834 29.38367266279646 29.40677756475964 20.679675541939066 62-63 20.484815205982166 29.337988395623142 29.33340350833405 20.84379289006064 64-65 20.33633308530558 29.213597669177815 29.437221833680894 21.012847411835715 66-67 20.351490233078465 29.307220701512442 29.1759556763551 21.165333389054 68-69 20.412079494727426 29.26330518453668 29.25760613196346 21.06700918877243 70-71 20.468683305462445 29.073842063077265 29.273346880252404 21.18412775120789 72-73 20.45270230245376 29.20339196009934 29.12856227930623 21.21534345814067 74-75 20.33503483778412 29.430391518765248 28.915219720861597 21.31935392258903 76-77 20.368379192812608 29.328986268033702 29.096749048606362 21.205885490547324 78-79 20.31428948933768 29.38547451022991 28.916868207937323 21.383367792495093 80-81 20.33531439825781 29.356149048964486 28.781699309744873 21.526837243032826 82-83 20.452175811486303 29.227499767900245 28.98568793966851 21.334636480944944 84-85 20.53912706046678 29.21489583220754 28.79573890143778 21.45023820588791 86-87 20.348161562081728 29.247198383329398 28.917040652966957 21.48759940162192 88-89 20.46940885443469 29.179288312437908 28.83809658772072 21.513206245406685 90-91 20.38558755668187 29.19510105998539 28.895193797229425 21.524117586103316 92-93 20.394236846315028 29.084808257653037 28.73990018855604 21.781054707475896 94-95 20.24728559750538 29.059659205001676 28.8185661114573 21.874489086035645 96-97 20.26660811454676 29.047841308077533 28.795092459863852 21.890458117511855 98-99 20.13372628869328 29.044193774969955 28.500310601877416 22.321769334459347 100-101 20.265096064807018 29.156343873507545 28.48513129141964 22.093428770265795 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 147650.0 1 134419.5 2 89705.5 3 45480.5 4 28198.5 5 22200.5 6 20679.0 7 20995.0 8 21562.0 9 21080.0 10 19078.0 11 16056.5 12 12625.0 13 9569.5 14 7267.5 15 5956.0 16 5742.5 17 6241.5 18 7259.0 19 8579.0 20 9996.0 21 11467.5 22 12992.0 23 14863.5 24 17085.5 25 19375.0 26 22378.0 27 26023.0 28 30028.5 29 34628.0 30 39698.0 31 44844.5 32 50151.0 33 55317.5 34 59958.5 35 63973.5 36 67855.0 37 71511.0 38 73554.5 39 74346.5 40 74321.5 41 73684.5 42 72779.0 43 71996.0 44 71384.0 45 70378.5 46 68696.0 47 66235.0 48 63353.5 49 60039.0 50 55957.0 51 51103.5 52 45867.0 53 40737.0 54 35621.0 55 30050.5 56 24368.0 57 19553.0 58 15644.5 59 12219.5 60 9288.5 61 6903.0 62 4935.5 63 3365.0 64 2215.5 65 1448.5 66 927.5 67 589.0 68 382.5 69 235.0 70 161.5 71 125.0 72 87.0 73 59.0 74 37.5 75 26.5 76 18.5 77 16.0 78 8.5 79 4.5 80 4.5 81 3.0 82 4.0 83 3.0 84 1.0 85 0.5 86 0.0 87 0.0 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.44086553963794 2 29.448163368714752 3 23.218809841869405 4 29.55670130229069 5 32.706974724110474 6 25.28514342307009 7 24.206755920849155 8 20.208044063357526 9 12.00127662654725 10-11 15.663763547705692 12-13 15.730288787402197 14-15 19.87389611286958 16-17 23.226110138652174 18-19 27.231854958114805 20-21 29.299525213374306 22-23 28.88404578746142 24-25 27.41393052914196 26-27 28.8424032494752 28-29 25.806651948171595 30-31 27.67805732316419 32-33 26.680487190960957 34-35 24.72049117219323 36-37 27.728768680534078 38-39 27.840185604390545 40-41 24.86330965432375 42-43 25.71997377651139 44-45 24.412171877364884 46-47 27.727370313826395 48-49 23.10885297736949 50-51 28.69440258483973 52-53 22.28671617433191 54-55 23.424966110171557 56-57 25.548941189631684 58-59 24.8362060172542 60-61 24.34685992642946 62-63 24.199825779959596 64-65 22.726996045089923 66-67 23.235713627659365 68-69 24.224502839506982 70-71 22.723705770483605 72-73 22.585000131610986 74-75 19.84226834821634 76-77 22.12175003125761 78-79 21.246166830083638 80-81 22.275138520560926 82-83 23.13883560471957 84-85 20.084699894053156 86-87 21.644166672150458 88-89 20.477476425182445 90-91 21.612394957983195 92-93 21.58473608707383 94-95 20.632304409626027 96-97 19.549002059711903 98-99 18.961852556214343 100-101 20.630720964971736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2431408.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.86789508440705 #Duplication Level Percentage of deduplicated Percentage of total 1 90.01601835325181 70.99373891400323 2 6.877838628708423 10.84881310752916 3 1.5305678468526231 3.621379930954185 4 0.5836709292448123 1.8413159044599294 5 0.28802379144895524 1.135791508290467 6 0.17628006434581941 0.8341702572179166 7 0.10962895203490323 0.605234328111157 8 0.07730907065284917 0.4877762938657537 9 0.059014956356391306 0.4188946847694058 >10 0.26818251328311943 3.590050263939836 >50 0.009800343798984893 0.5259838193232464 >100 0.0034559271223560603 0.4145127150525112 >500 5.215572474604361E-5 0.03360657789722221 >1k 1.0431144949208721E-4 0.1368120906807357 >5k 0.0 0.0 >10k+ 5.2155724746043606E-5 4.511919603905152 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 109688 4.511295512723492 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 4.112843257898304E-5 0.0 0.0 0.0 0.0 10-11 4.112843257898304E-5 0.0 0.0 0.0 0.0 12-13 4.112843257898304E-5 0.0 0.0 4.112843257898304E-5 0.0 14-15 4.112843257898304E-5 0.0 0.0 4.112843257898304E-5 0.0 16-17 4.112843257898304E-5 0.0 0.0 4.112843257898304E-5 0.0 18-19 4.112843257898304E-5 0.0 0.0 4.112843257898304E-5 0.0 20-21 4.112843257898304E-5 0.0 0.0 8.225686515796608E-5 0.0 22-23 4.112843257898304E-5 0.0 0.0 1.4394951402644065E-4 0.0 24-25 4.112843257898304E-5 0.0 0.0 1.6451373031593216E-4 0.0 26-27 4.112843257898304E-5 0.0 0.0 2.2620637918440676E-4 0.0 28-29 4.112843257898304E-5 0.0 0.0 5.14105407237288E-4 0.0 30-31 4.112843257898304E-5 0.0 0.0 0.0014806235728433894 0.0 32-33 4.112843257898304E-5 0.0 0.0 0.0035781736343715245 0.0 34-35 4.112843257898304E-5 0.0 0.0 0.007300296782769489 0.0 36-37 4.112843257898304E-5 0.0 0.0 0.01351069010219593 0.0 38-39 4.112843257898304E-5 0.0 0.0 0.022127096727492877 0.0 40-41 4.112843257898304E-5 0.0 0.0 0.03265597546771253 0.0 42-43 4.112843257898304E-5 0.0 0.0 0.04240341398893152 0.0 44-45 4.112843257898304E-5 0.0 0.0 0.05490645749294236 0.0 46-47 4.112843257898304E-5 0.0 0.0 0.07113162414535118 0.0 48-49 4.112843257898304E-5 0.0 0.0 0.088528951126261 0.0 50-51 4.112843257898304E-5 0.0 0.0 0.10896978211801556 0.0 52-53 4.112843257898304E-5 0.0 0.0 0.1331327362581681 0.0 54-55 4.112843257898304E-5 2.056421628949152E-5 0.0 0.15929041937840133 0.0 56-57 4.112843257898304E-5 4.112843257898304E-5 0.0 0.19188470219724538 0.0 58-59 4.112843257898304E-5 4.112843257898304E-5 0.0 0.22900311259977757 0.0 60-61 4.112843257898304E-5 4.112843257898304E-5 0.0 0.2683424583615749 0.0 62-63 4.112843257898304E-5 4.112843257898304E-5 0.0 0.3087100149378467 4.112843257898304E-5 64-65 4.112843257898304E-5 4.112843257898304E-5 0.0 0.3524706672018847 4.112843257898304E-5 66-67 4.112843257898304E-5 4.112843257898304E-5 0.0 0.40497111138895653 4.112843257898304E-5 68-69 4.112843257898304E-5 4.112843257898304E-5 0.0 0.46600570533616736 6.169264886847457E-5 70-71 4.112843257898304E-5 4.112843257898304E-5 0.0 0.5327571514118569 8.225686515796608E-5 72-73 4.112843257898304E-5 4.112843257898304E-5 0.0 0.6059246329698678 8.225686515796608E-5 74-75 4.112843257898304E-5 4.112843257898304E-5 0.0 0.6785780091206413 8.225686515796608E-5 76-77 4.112843257898304E-5 4.112843257898304E-5 0.0 0.759230865408027 8.225686515796608E-5 78-79 4.112843257898304E-5 4.112843257898304E-5 0.0 0.8520166093062127 8.225686515796608E-5 80-81 4.112843257898304E-5 4.112843257898304E-5 0.0 0.9568118555174614 8.225686515796608E-5 82-83 4.112843257898304E-5 4.112843257898304E-5 0.0 1.065987279798372 8.225686515796608E-5 84-85 4.112843257898304E-5 4.112843257898304E-5 0.0 1.174771983969782 8.225686515796608E-5 86-87 4.112843257898304E-5 4.112843257898304E-5 0.0 1.297355277271441 8.225686515796608E-5 88-89 4.112843257898304E-5 4.112843257898304E-5 0.0 1.4332641827286907 8.225686515796608E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1865 0.0 56.636986 1 GTATCAA 3840 0.0 47.403336 1 ATCAACG 4515 0.0 39.740692 3 TCAACGC 4660 0.0 38.60989 4 CAACGCA 4685 0.0 38.399097 5 TATCAAC 4750 0.0 37.885277 2 AACGCAG 5120 0.0 34.914574 6 ACGCAGA 5650 0.0 31.134974 7 CGCAGAG 5775 0.0 30.022972 8 GCAGAGT 6285 0.0 25.939678 9 GTGGTAT 1070 0.0 23.960617 1 TACATGG 3730 0.0 22.129622 2 GTACATG 3740 0.0 21.936136 1 GAGTACT 4160 0.0 21.766634 12-13 CAGAGTA 5865 0.0 21.470871 10-11 AGAGTAC 5330 0.0 21.263412 10-11 ACATGGG 3690 0.0 21.171349 3 GTACTTT 4700 0.0 19.345446 14-15 TGGTATC 1105 0.0 19.02298 2 AGTACTT 4710 0.0 18.71494 12-13 >>END_MODULE