FastQCFastQC Report
Sat 18 Jun 2016
SRR1931034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431408
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59500.24471417384494912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52070.21415574843876467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32690.13444884610069557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31500.060.052591
GTATCAA60750.039.5097541
ATCAACG74200.031.961643
TCAACGC75300.031.6827754
CAACGCA76050.031.1907235
TATCAAC78000.030.3442252
AACGCAG79800.029.843286
TAGTCCG909.015507E-426.3561635
ACGCAGA92850.025.495557
CGCAGAG93800.025.035038
TTCGCGC1050.002213085522.5905328
GTACCGT1754.0968807E-621.686916
GCAGAGT104150.021.6361569
GCGTGCG5500.020.7011419
AGAGTAC98150.019.79289810-11
CGTTCGC1200.00479989719.7667166
GTTCGCG1200.00479989719.7667167
GAGTACT76350.019.7589512-13
GTACATG62400.019.6514381
TGGTATC15300.019.2593482
Produced by FastQC (version 0.11.5)