FastQCFastQC Report
Sat 18 Jun 2016
SRR1931033_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931033_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247670
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN544204.3617302652143595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.10154928787259451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10850.060.8679471
GTATCAA19350.038.6278531
ATCAACG22900.032.7562943
TAACGCA1905.456968E-1231.7902284
TCAACGC23850.031.445894
ATAACGC2100.031.1650643
CAACGCA24700.030.7621125
TATCAAC23900.030.4153392
AACGCAG27550.028.0313196
TATAACG1854.0345185E-927.6713622
GTACATG17500.025.158751
ACGCAGA30350.024.8730537
TACATGG17150.024.7751452
CGCAGAG30850.024.6162458
ACATGGG18050.023.9864083
GTGGTAT5850.023.6285231
GTAGCCC3650.022.0684133
TGGTATC5350.022.0077822
GCAGAGT32950.021.2651189
CAGAGTA30250.020.68198210-11
Produced by FastQC (version 0.11.5)