##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931033_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1247670 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.079824793414925 28.0 2.0 31.0 2.0 33.0 2 19.79857895116497 27.0 2.0 31.0 2.0 33.0 3 20.438332251316453 27.0 2.0 31.0 2.0 33.0 4 21.985532232080597 30.0 2.0 35.0 2.0 37.0 5 21.360871063662668 30.0 2.0 35.0 2.0 37.0 6 22.318714884544793 30.0 2.0 35.0 2.0 37.0 7 22.49904702365209 30.0 2.0 35.0 2.0 37.0 8 22.912390295510832 32.0 2.0 35.0 2.0 37.0 9 24.181338014058205 32.0 2.0 37.0 2.0 39.0 10-11 24.535642437503505 32.0 2.0 37.0 2.0 39.0 12-13 24.42957633027964 32.0 2.0 37.0 2.0 39.0 14-15 24.40263531222198 32.0 2.0 38.0 2.0 40.0 16-17 23.252941883671163 31.5 2.0 38.0 2.0 40.0 18-19 22.214323899749132 28.5 2.0 38.0 2.0 40.0 20-21 21.880089286429907 28.0 2.0 37.5 2.0 40.0 22-23 21.502930662755375 27.0 2.0 37.0 2.0 40.0 24-25 21.333234749573204 27.0 2.0 37.0 2.0 39.5 26-27 21.0442096067069 27.0 2.0 37.0 2.0 39.0 28-29 20.82604895525259 26.0 2.0 37.0 2.0 39.0 30-31 20.78933131356849 26.0 2.0 36.0 2.0 39.0 32-33 20.640657786113316 25.5 2.0 36.0 2.0 39.0 34-35 20.31414276210857 25.0 2.0 36.0 2.0 39.0 36-37 19.84467487396507 24.0 2.0 36.0 2.0 39.0 38-39 19.70539044779469 24.0 2.0 35.0 2.0 39.0 40-41 19.554104049949103 24.0 2.0 35.0 2.0 39.0 42-43 19.392815407920363 23.5 2.0 35.0 2.0 39.0 44-45 18.993281476672518 22.0 2.0 35.0 2.0 38.0 46-47 18.638752234164482 19.0 2.0 35.0 2.0 38.0 48-49 18.369030272427807 18.0 2.0 34.5 2.0 38.0 50-51 16.147000408761933 10.0 2.0 32.5 2.0 37.5 52-53 18.01124696434153 17.5 2.0 33.5 2.0 38.0 54-55 18.752878565646363 21.0 2.0 35.5 2.0 39.0 56-57 18.716445053579875 20.0 2.0 35.5 2.0 39.0 58-59 18.427901207851434 18.5 2.0 35.0 2.0 39.0 60-61 18.122589707214246 16.0 2.0 35.0 2.0 39.0 62-63 17.675336427100113 12.0 2.0 34.0 2.0 38.5 64-65 17.317647695304046 8.5 2.0 34.0 2.0 38.0 66-67 16.94610594147491 8.0 2.0 34.0 2.0 38.0 68-69 16.500161901784928 6.5 2.0 33.0 2.0 37.5 70-71 16.130613062748964 2.0 2.0 32.5 2.0 37.0 72-73 15.715956142249151 2.0 2.0 32.0 2.0 36.0 74-75 15.268234789647904 2.0 2.0 31.5 2.0 36.0 76-77 14.83502528713522 2.0 2.0 31.0 2.0 35.5 78-79 14.450643198922792 2.0 2.0 31.0 2.0 35.0 80-81 14.016818549776783 2.0 2.0 30.0 2.0 34.5 82-83 13.556270888936979 2.0 2.0 30.0 2.0 34.0 84-85 13.151252735098222 2.0 2.0 29.0 2.0 34.0 86-87 12.659668421938493 2.0 2.0 29.0 2.0 34.0 88-89 12.239750494922536 2.0 2.0 29.0 2.0 34.0 90-91 11.752637716703937 2.0 2.0 27.5 2.0 34.0 92-93 11.21418443979578 2.0 2.0 26.0 2.0 34.0 94-95 10.64830083275225 2.0 2.0 25.5 2.0 33.0 96-97 10.007171367428889 2.0 2.0 24.0 2.0 33.0 98-99 9.34577292072423 2.0 2.0 19.0 2.0 32.0 100-101 8.454736428703104 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.959606552314412 1101 2 9.41827398903931 1101 3 8.57462682211704 1101 4 10.840814227601587 1101 5 11.83127452665882 1101 6 10.78820983072342 1101 7 10.650528582465231 1101 8 10.034078663538441 1101 9 9.523854135008492 1101 10-11 9.359612954548627 1101 12-13 9.00098347188288 1101 14-15 9.75934966295127 1101 16-17 10.904581049675482 1101 18-19 11.772706872898343 1101 20-21 11.661578076446403 1101 22-23 11.74498085229963 1101 24-25 11.859278068693378 1101 26-27 11.778499021517195 1101 28-29 11.813624059300164 1101 30-31 11.280561055468088 1101 32-33 11.46412930643384 1101 34-35 11.326607326898298 1101 36-37 11.983618083477413 1101 38-39 11.914921801189937 1101 40-41 11.149900736974153 1101 42-43 10.853776783624436 1101 44-45 10.7482716505523 1101 46-47 10.618603702089441 1101 48-49 10.885762897298754 1101 50-51 12.09774427134717 1101 52-53 11.376280430932166 1101 54-55 10.982840574643895 1101 56-57 11.179308254525651 1101 58-59 10.912755561294535 1101 60-61 10.565228151134257 1101 62-63 10.716064655193293 1101 64-65 10.41217327628512 1101 66-67 10.235226629122 1101 68-69 9.92953185484576 1101 70-71 9.653644929581018 1101 72-73 9.794834035544879 1101 74-75 9.365854537205637 1101 76-77 9.095989016391451 1101 78-79 9.084922279739246 1101 80-81 8.968162217608421 1101 82-83 8.747662810074038 1101 84-85 8.56853420010032 1101 86-87 7.92811180145798 1101 88-89 7.574989071954633 1101 90-91 7.444072604488353 1101 92-93 7.285976188161413 1101 94-95 7.0664057261979725 1101 96-97 6.767831698754309 1101 98-99 6.652314442311647 1101 100-101 5.93311276301675 1102 1 7.626033583227425 1102 2 8.81389105110497 1102 3 8.43750484751843 1102 4 10.275192372820854 1102 5 11.04388655637365 1102 6 10.075578630148296 1102 7 10.252812311197555 1102 8 9.58652055186818 1102 9 9.298265829233266 1102 10-11 8.506758892722605 1102 12-13 8.743222868000775 1102 14-15 9.256511177449237 1102 16-17 10.057146350418346 1102 18-19 11.005143968513007 1102 20-21 11.094670575847317 1102 22-23 11.528918067732175 1102 24-25 11.281573779213396 1102 26-27 11.3001453191448 1102 28-29 10.848153175044231 1102 30-31 11.058270137663154 1102 32-33 11.010527581056412 1102 34-35 10.58717047091939 1102 36-37 10.68764995503265 1102 38-39 10.761195465220606 1102 40-41 10.359038048260999 1102 42-43 10.263032714609341 1102 44-45 10.257470068956327 1102 46-47 10.301210939089199 1102 48-49 9.4979855189139 1102 50-51 11.232259008946018 1102 52-53 10.60168182081954 1102 54-55 10.442436788398986 1102 56-57 10.017204252608565 1102 58-59 10.276433970113132 1102 60-61 10.020651108239456 1102 62-63 9.855798659506736 1102 64-65 9.768458922103477 1102 66-67 9.773178941607021 1102 68-69 9.592994591486065 1102 70-71 9.290361919755954 1102 72-73 9.086185581195489 1102 74-75 8.910412409240143 1102 76-77 8.688458462832857 1102 78-79 8.654439413695634 1102 80-81 8.195033772844466 1102 82-83 8.127436354286838 1102 84-85 8.213776232211508 1102 86-87 7.776598501673654 1102 88-89 7.528989790861893 1102 90-91 7.354560263247043 1102 92-93 7.231989847399369 1102 94-95 6.775475939473795 1102 96-97 6.877775384352203 1102 98-99 6.243858894743951 1102 100-101 5.2298273568341465 1103 1 8.098195315909432 1103 2 9.402012998018044 1103 3 8.894971741756557 1103 4 10.919903102030435 1103 5 11.58395718199898 1103 6 10.726586222403018 1103 7 10.263826377467932 1103 8 10.09356663508871 1103 9 10.016055247160402 1103 10-11 9.079853177932417 1103 12-13 8.983455638290923 1103 14-15 9.730209900302508 1103 16-17 10.541880327229602 1103 18-19 11.404490975752136 1103 20-21 11.836643384981564 1103 22-23 11.910948848733877 1103 24-25 12.037292689224572 1103 26-27 11.907048385474031 1103 28-29 11.802119686726886 1103 30-31 11.565636684658621 1103 32-33 11.718591261828585 1103 34-35 11.60605635167979 1103 36-37 11.544781671823895 1103 38-39 11.483666185013437 1103 40-41 11.235602994408957 1103 42-43 10.953683647456142 1103 44-45 10.887499496300212 1103 46-47 10.963953567539559 1103 48-49 10.79652974821176 1103 50-51 12.472026532929304 1103 52-53 11.459271132808237 1103 54-55 11.193792506131903 1103 56-57 11.265626652702732 1103 58-59 10.883728420116075 1103 60-61 10.642367899481876 1103 62-63 10.735146843839619 1103 64-65 10.447413368575138 1103 66-67 10.289180059846089 1103 68-69 10.107637049257828 1103 70-71 10.013239325441317 1103 72-73 9.681426002595055 1103 74-75 9.476904669848702 1103 76-77 9.210484829732986 1103 78-79 8.826700316526544 1103 80-81 8.44506349446179 1103 82-83 8.433045794578755 1103 84-85 8.285741390236346 1103 86-87 7.9499773492988695 1103 88-89 7.546351040935463 1103 90-91 6.90476890311521 1103 92-93 6.696998969875974 1103 94-95 6.564215566335074 1103 96-97 6.069037523131554 1103 98-99 5.673387468694866 1103 100-101 5.1468066399840495 1104 1 8.05687817194692 1104 2 9.336792911866667 1104 3 8.665144984322094 1104 4 10.83232413329851 1104 5 11.481237434583608 1104 6 10.643283111047953 1104 7 10.281305731868823 1104 8 9.734130545491741 1104 9 9.354468947893054 1104 10-11 8.792895638418504 1104 12-13 8.740700985211372 1104 14-15 9.602549957809796 1104 16-17 10.518743649125671 1104 18-19 11.255338449992347 1104 20-21 11.476356275227062 1104 22-23 11.675326102049024 1104 24-25 11.453396861933236 1104 26-27 11.346652188311804 1104 28-29 11.152225112902908 1104 30-31 11.011059251337723 1104 32-33 10.811890400603104 1104 34-35 10.825782145604911 1104 36-37 11.056238732494826 1104 38-39 10.94170673992932 1104 40-41 10.219313684455997 1104 42-43 9.943448342688871 1104 44-45 10.337791789436299 1104 46-47 10.194043818342578 1104 48-49 9.533169856053775 1104 50-51 11.4131349001987 1104 52-53 10.346322711202706 1104 54-55 10.334146544355164 1104 56-57 10.054514843298957 1104 58-59 10.215535471475373 1104 60-61 10.13371643778557 1104 62-63 9.975490561425463 1104 64-65 9.42801575938827 1104 66-67 9.289721460881264 1104 68-69 9.225055921264051 1104 70-71 8.939745143916872 1104 72-73 8.714556690116714 1104 74-75 8.307489100446452 1104 76-77 8.17551718406641 1104 78-79 8.0273444626783 1104 80-81 7.9776582583815365 1104 82-83 7.560437919413493 1104 84-85 7.193276551033609 1104 86-87 6.776637919678407 1104 88-89 6.320803888595357 1104 90-91 6.291131766348313 1104 92-93 6.1036882996630055 1104 94-95 5.608324759832033 1104 96-97 4.665691157350869 1104 98-99 4.344045550589989 1104 100-101 3.9822530133178287 1105 1 7.755146187490631 1105 2 8.901015703560951 1105 3 8.437231446220935 1105 4 10.329068647148937 1105 5 10.596965796410721 1105 6 10.457707916524566 1105 7 9.827045397388474 1105 8 9.577361608402011 1105 9 9.213629591976549 1105 10-11 8.927042720340769 1105 12-13 8.562828984775567 1105 14-15 9.106659462898723 1105 16-17 10.38371956635903 1105 18-19 10.555434747011613 1105 20-21 11.203506303372805 1105 22-23 11.485813341133468 1105 24-25 11.186241295432112 1105 26-27 11.252998655567414 1105 28-29 11.281012303867218 1105 30-31 10.595930007913385 1105 32-33 10.583010203855302 1105 34-35 10.478160971382778 1105 36-37 10.553677526858415 1105 38-39 10.202894955489374 1105 40-41 9.859927760957937 1105 42-43 9.908908525545392 1105 44-45 9.776140133831209 1105 46-47 9.438498937235632 1105 48-49 9.59797740955338 1105 50-51 11.094837785591062 1105 52-53 10.13969155205216 1105 54-55 9.729776918148755 1105 56-57 9.512876171051566 1105 58-59 9.262454591058454 1105 60-61 9.154939801473933 1105 62-63 9.145883228552147 1105 64-65 9.053153083363906 1105 66-67 8.866291777566243 1105 68-69 8.778650291208034 1105 70-71 8.372593152378753 1105 72-73 8.482207360620885 1105 74-75 8.218485856876844 1105 76-77 8.027806238551117 1105 78-79 7.913535799237803 1105 80-81 7.624367646801835 1105 82-83 7.392290385797493 1105 84-85 7.053560754917719 1105 86-87 6.572098965065685 1105 88-89 6.415107724133442 1105 90-91 6.067655722005155 1105 92-93 5.939119133775645 1105 94-95 5.775875615099375 1105 96-97 5.5862983222576705 1105 98-99 4.906566262677222 1105 100-101 4.433492787788735 1106 1 7.723796031781131 1106 2 9.04666480335122 1106 3 8.681723733380121 1106 4 10.340236718076202 1106 5 11.051697118080217 1106 6 10.276289893263478 1106 7 9.458396914988644 1106 8 9.315140342025277 1106 9 8.795312956936009 1106 10-11 8.61090618853154 1106 12-13 8.47581779003428 1106 14-15 8.94128394424536 1106 16-17 9.98747009976621 1106 18-19 11.273047568304325 1106 20-21 11.246137737171296 1106 22-23 11.24842331380486 1106 24-25 11.249553606469782 1106 26-27 11.473253544754908 1106 28-29 11.151848323151587 1106 30-31 10.998248399792882 1106 32-33 11.043239307912827 1106 34-35 11.140401587956081 1106 36-37 11.474475356458655 1106 38-39 10.947358586199101 1106 40-41 11.007181054463537 1106 42-43 10.722649548416076 1106 44-45 9.947814396821375 1106 46-47 10.037676071545288 1106 48-49 10.35457151392868 1106 50-51 12.084197447297418 1106 52-53 11.370437255287104 1106 54-55 10.73505083975833 1106 56-57 10.56355779733061 1106 58-59 10.503402841482906 1106 60-61 10.626557432510513 1106 62-63 10.607743764138352 1106 64-65 10.240374463231529 1106 66-67 9.697407971808172 1106 68-69 9.286849440480328 1106 70-71 8.745321632236388 1106 72-73 8.647864904133295 1106 74-75 8.50568050964012 1106 76-77 8.138214998685855 1106 78-79 7.901873475489154 1106 80-81 7.7293538122624685 1106 82-83 7.686662486105279 1106 84-85 7.385816113957496 1106 86-87 7.1519205119918965 1106 88-89 6.7665461644054545 1106 90-91 6.457672836431206 1106 92-93 6.4819852099870445 1106 94-95 6.3943081810829785 1106 96-97 5.756120697667372 1106 98-99 5.498152558879363 1106 100-101 4.849246231102304 1107 1 7.57844068026262 1107 2 8.432323692843397 1107 3 8.383644823245184 1107 4 10.080037775352206 1107 5 11.071582331672733 1107 6 9.882369938013984 1107 7 9.84326598975041 1107 8 8.154298393860902 1107 9 5.831449670185702 1107 10-11 7.644953714429821 1107 12-13 7.7753696055607335 1107 14-15 8.304150026203782 1107 16-17 9.215026197843653 1107 18-19 10.552567247713146 1107 20-21 10.976447146384217 1107 22-23 10.439269162917977 1107 24-25 10.395072016513282 1107 26-27 10.80496355875643 1107 28-29 10.501674818689843 1107 30-31 10.22994239102962 1107 32-33 10.813910231463858 1107 34-35 10.814464581767218 1107 36-37 11.042260427205104 1107 38-39 10.8954496407971 1107 40-41 10.178323432298448 1107 42-43 10.047899500715797 1107 44-45 9.509472033933495 1107 46-47 9.85936732815468 1107 48-49 9.797450640569103 1107 50-51 11.155212831982483 1107 52-53 10.25644689046064 1107 54-55 10.281621033905548 1107 56-57 9.991641344442353 1107 58-59 10.14433664594969 1107 60-61 9.860792702182703 1107 62-63 9.885802994015204 1107 64-65 9.942463141025051 1107 66-67 9.720406301024465 1107 68-69 9.456230446540104 1107 70-71 9.079330884675331 1107 72-73 8.787629839416608 1107 74-75 8.87151221852177 1107 76-77 8.799754191897184 1107 78-79 8.556606956896236 1107 80-81 8.375720070018366 1107 82-83 8.13203844640959 1107 84-85 7.782179399292364 1107 86-87 7.443421595934765 1107 88-89 7.261030766415267 1107 90-91 6.928919333928425 1107 92-93 6.346594829194434 1107 94-95 6.197002553355782 1107 96-97 6.023516296332783 1107 98-99 5.941047821519653 1107 100-101 5.554368476871135 1108 1 6.818751063616137 1108 2 8.25023105812991 1108 3 7.7493910435142155 1108 4 9.198536243561986 1108 5 11.06956738999969 1108 6 9.496228436962486 1108 7 8.739715484128126 1108 8 5.907234753952249 1108 9 7.287079785444483 1108 10-11 6.380479183086727 1108 12-13 5.097841179673882 1108 14-15 7.618952929000574 1108 16-17 8.615057016616685 1108 18-19 8.108563357343353 1108 20-21 7.983952861293986 1108 22-23 9.257326333743176 1108 24-25 9.014031336876428 1108 26-27 7.0691290447038675 1108 28-29 9.089148960081111 1108 30-31 9.615542705278184 1108 32-33 10.097193076899991 1108 34-35 9.580600982569077 1108 36-37 9.349402091784786 1108 38-39 8.97050936289067 1108 40-41 9.058308040065235 1108 42-43 8.561633581018427 1108 44-45 7.712110040856569 1108 46-47 8.647793306493487 1108 48-49 9.202623734579511 1108 50-51 10.65634752317152 1108 52-53 9.82712966065094 1108 54-55 9.364083551073147 1108 56-57 9.450519438184024 1108 58-59 9.435846216922638 1108 60-61 9.32329014348123 1108 62-63 9.080772901398785 1108 64-65 8.504598132968713 1108 66-67 8.129211607571197 1108 68-69 8.338257441269466 1108 70-71 8.385568570031095 1108 72-73 7.9187262672978225 1108 74-75 7.865367332551235 1108 76-77 7.5804500329066205 1108 78-79 7.412270463923946 1108 80-81 7.379643888755949 1108 82-83 7.273070354989331 1108 84-85 6.973378152361377 1108 86-87 6.518769207679977 1108 88-89 6.607286828679481 1108 90-91 6.378719612269405 1108 92-93 6.061543763802604 1108 94-95 5.580472427004528 1108 96-97 5.446237508225284 1108 98-99 5.217441613395163 1108 100-101 4.6327830997213955 1109 1 6.160118563808261 1109 2 7.757290274338409 1109 3 7.531007832852612 1109 4 8.715519310389691 1109 5 9.588999303460778 1109 6 8.601836238402889 1109 7 7.514161798183817 1109 8 8.235260914745698 1109 9 7.349947470877229 1109 10-11 5.241275442487833 1109 12-13 6.06398659184287 1109 14-15 7.4721807038535 1109 16-17 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72-73 -13.34535288034297 2313 74-75 -12.888818359990625 2313 76-77 -12.484618460244063 2313 78-79 -12.110560586304366 2313 80-81 -11.692273919463227 2313 82-83 -11.239486722636242 2313 84-85 -10.849300690865412 2313 86-87 -10.351093806018653 2313 88-89 -9.943702516830413 2313 90-91 -9.485055121368342 2313 92-93 -8.944163998754476 2313 94-95 -8.38413944514159 2313 96-97 -7.7679938464170295 2313 98-99 -7.128448797563742 2313 100-101 -6.2713264893197 2314 1 -18.643197186003345 2314 2 -17.375339718125797 2314 3 -18.00403361402003 2314 4 -19.49096147333299 2314 5 -18.831498059010965 2314 6 -19.80057521600555 2314 7 -19.976418458033212 2314 8 -20.45501790967028 2314 9 -21.680195709228272 2314 10-11 -22.05977956881586 2314 12-13 -21.973315593537688 2314 14-15 -21.94002728408923 2314 16-17 -20.793238264966266 2314 18-19 -19.71677910841007 2314 20-21 -19.36840634722961 2314 22-23 -18.99762039380629 2314 24-25 -18.82919545155584 2314 26-27 -18.525239296239814 2314 28-29 -18.31838528649423 2314 30-31 -18.280576016183 2314 32-33 -18.14101088048205 2314 34-35 -17.815137221388305 2314 36-37 -17.345042352659657 2314 38-39 -17.200342996317854 2314 40-41 -17.045192720969773 2314 42-43 -16.925428823852197 2314 44-45 -16.535606854120598 2314 46-47 -16.16955829168003 2314 48-49 -15.902399096470717 2314 50-51 -13.78543329874629 2314 52-53 -15.559856640718923 2314 54-55 -16.26871705775486 2314 56-57 -16.24164190123759 2314 58-59 -15.97587372219456 2314 60-61 -15.681656584068236 2314 62-63 -15.243047494339418 2314 64-65 -14.889618000973446 2314 66-67 -14.524897981469902 2314 68-69 -14.101620793129317 2314 70-71 -13.738099618705585 2314 72-73 -13.34535288034297 2314 74-75 -12.888818359990625 2314 76-77 -12.484618460244063 2314 78-79 -12.110560586304366 2314 80-81 -11.692273919463227 2314 82-83 -11.239486722636242 2314 84-85 -10.849300690865412 2314 86-87 -10.351093806018653 2314 88-89 -9.943702516830413 2314 90-91 -9.485055121368342 2314 92-93 -8.944163998754476 2314 94-95 -8.38413944514159 2314 96-97 -7.7679938464170295 2314 98-99 -7.128448797563742 2314 100-101 -6.2713264893197 2315 1 -18.643197186003345 2315 2 -17.375339718125797 2315 3 -18.00403361402003 2315 4 -19.49096147333299 2315 5 -18.831498059010965 2315 6 -19.80057521600555 2315 7 -19.976418458033212 2315 8 -20.45501790967028 2315 9 -21.680195709228272 2315 10-11 -22.05977956881586 2315 12-13 -21.973315593537688 2315 14-15 -21.94002728408923 2315 16-17 -20.793238264966266 2315 18-19 -19.71677910841007 2315 20-21 -19.36840634722961 2315 22-23 -18.99762039380629 2315 24-25 -18.82919545155584 2315 26-27 -18.525239296239814 2315 28-29 -18.31838528649423 2315 30-31 -18.280576016183 2315 32-33 -18.14101088048205 2315 34-35 -17.815137221388305 2315 36-37 -17.345042352659657 2315 38-39 -17.200342996317854 2315 40-41 -17.045192720969773 2315 42-43 -16.925428823852197 2315 44-45 -16.535606854120598 2315 46-47 -16.16955829168003 2315 48-49 -15.902399096470717 2315 50-51 -13.78543329874629 2315 52-53 -15.559856640718923 2315 54-55 -16.26871705775486 2315 56-57 -16.24164190123759 2315 58-59 -15.97587372219456 2315 60-61 -15.681656584068236 2315 62-63 -15.243047494339418 2315 64-65 -14.889618000973446 2315 66-67 -14.524897981469902 2315 68-69 -14.101620793129317 2315 70-71 -13.738099618705585 2315 72-73 -13.34535288034297 2315 74-75 -12.888818359990625 2315 76-77 -12.484618460244063 2315 78-79 -12.110560586304366 2315 80-81 -11.692273919463227 2315 82-83 -11.239486722636242 2315 84-85 -10.849300690865412 2315 86-87 -10.351093806018653 2315 88-89 -9.943702516830413 2315 90-91 -9.485055121368342 2315 92-93 -8.944163998754476 2315 94-95 -8.38413944514159 2315 96-97 -7.7679938464170295 2315 98-99 -7.128448797563742 2315 100-101 -6.2713264893197 2316 1 -18.643197186003345 2316 2 -17.375339718125797 2316 3 -18.00403361402003 2316 4 -19.49096147333299 2316 5 -18.831498059010965 2316 6 -19.80057521600555 2316 7 -19.976418458033212 2316 8 -20.45501790967028 2316 9 -21.680195709228272 2316 10-11 -22.05977956881586 2316 12-13 -21.973315593537688 2316 14-15 -21.94002728408923 2316 16-17 -20.793238264966266 2316 18-19 -19.71677910841007 2316 20-21 -19.36840634722961 2316 22-23 -18.99762039380629 2316 24-25 -18.82919545155584 2316 26-27 -18.525239296239814 2316 28-29 -18.31838528649423 2316 30-31 -18.280576016183 2316 32-33 -18.14101088048205 2316 34-35 -17.815137221388305 2316 36-37 -17.345042352659657 2316 38-39 -17.200342996317854 2316 40-41 -17.045192720969773 2316 42-43 -16.925428823852197 2316 44-45 -16.535606854120598 2316 46-47 -16.16955829168003 2316 48-49 -15.902399096470717 2316 50-51 -13.78543329874629 2316 52-53 -15.559856640718923 2316 54-55 -16.26871705775486 2316 56-57 -16.24164190123759 2316 58-59 -15.97587372219456 2316 60-61 -15.681656584068236 2316 62-63 -15.243047494339418 2316 64-65 -14.889618000973446 2316 66-67 -14.524897981469902 2316 68-69 -14.101620793129317 2316 70-71 -13.738099618705585 2316 72-73 -13.34535288034297 2316 74-75 -12.888818359990625 2316 76-77 -12.484618460244063 2316 78-79 -12.110560586304366 2316 80-81 -11.692273919463227 2316 82-83 -11.239486722636242 2316 84-85 -10.849300690865412 2316 86-87 -10.351093806018653 2316 88-89 -9.943702516830413 2316 90-91 -9.485055121368342 2316 92-93 -8.944163998754476 2316 94-95 -8.38413944514159 2316 96-97 -7.7679938464170295 2316 98-99 -7.128448797563742 2316 100-101 -6.2713264893197 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 319251.0 3 13740.0 4 47426.0 5 26298.0 6 15603.0 7 15493.0 8 15137.0 9 15083.0 10 15949.0 11 16877.0 12 17194.0 13 17956.0 14 18128.0 15 18602.0 16 19279.0 17 19526.0 18 18670.0 19 19275.0 20 20339.0 21 21445.0 22 22726.0 23 24196.0 24 25894.0 25 27443.0 26 29066.0 27 31184.0 28 33585.0 29 36304.0 30 39017.0 31 42262.0 32 45467.0 33 48531.0 34 50154.0 35 47455.0 36 35897.0 37 15250.0 38 1955.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.359522985560794 20.629447108102532 14.047958168302344 22.963071738034333 2 14.840104174296986 21.28349405370318 41.58407138486227 22.292330387137564 3 18.21454604651259 27.007124437619435 30.788748137495116 23.989581378372858 4 12.153324595538306 17.838197259673947 40.183132443071685 29.82534570171606 5 12.359703076292963 38.054651619825286 36.01421588936063 13.571429414521122 6 28.465972658788928 39.16260881610212 18.956963697776263 13.414454827332687 7 25.178268001655084 33.651957849988136 23.812089248470414 17.35768489988637 8 24.070949487527134 36.889959605197205 21.438198747221985 17.600892160053675 9 25.22079456103728 17.273097583257808 21.900915293816663 35.60519256188825 10-11 23.336376707642685 27.46705992547437 29.829260554606503 19.367302812276446 12-13 24.101327722000484 25.207502412598615 29.87921151686311 20.811958348537793 14-15 21.506143140727662 26.813544021653396 27.650378680621873 24.02993415699707 16-17 20.941217471395987 29.7804303022571 28.197420841747807 21.080931384599108 18-19 20.710650021834454 28.61291911085946 30.376126496643945 20.300304370662143 20-21 21.288140243868153 27.996482432968868 30.4906601173347 20.224717205828277 22-23 20.98433766923281 27.823732413060792 30.3013147415925 20.890615176113897 24-25 20.749683075867857 28.00957401233073 30.455888653075174 20.784854258726234 26-27 20.737445141214742 28.168005780815225 30.175699004172984 20.918850073797046 28-29 20.87687229607889 28.322075152513754 30.172836407208237 20.62821614419911 30-31 21.056153076882055 28.10221325711157 30.18781100476496 20.65382266124142 32-33 20.880190346614413 28.201493565603077 30.092699582396193 20.825616505386318 34-35 20.97594948021243 28.27952139037292 30.117378550982753 20.627150578431895 36-37 21.061574396320008 28.197777588251316 29.897518150979835 20.84312986444884 38-39 21.061948950547286 28.252355717470383 29.92810805390353 20.757587278078795 40-41 21.19759223337988 28.16029959928807 29.951350066114557 20.690758101217494 42-43 21.05844963382617 28.27631047598849 29.695321094333277 20.969918795852067 44-45 21.055872419234877 28.453047404538818 29.669238564990298 20.821841611236007 46-47 21.202919235725425 28.413688979466972 29.273019188296644 21.11037259651096 48-49 21.080191316230824 28.606693997556782 29.187837753897757 21.125276932314637 50-51 21.33685115710398 28.842639327640306 28.852349056956488 20.968160458299224 52-53 20.93955138168417 28.9460041249197 28.98995818728939 21.124486306106746 54-55 20.825557095644733 28.89460229059248 29.16376275835689 21.116077855405894 56-57 20.89072408672218 29.0070747268356 29.094607037106112 21.007594149336107 58-59 20.91015297671762 28.969234735280246 29.12766904644566 20.99294324155648 60-61 20.8955223880597 29.1304251715539 29.096688080859543 20.877364359526858 62-63 20.862256335410574 28.956724433921156 29.09485134688178 21.086167883786494 64-65 20.63288668411237 28.879871325470237 29.14902482073172 21.338217169685674 66-67 20.63209949257212 28.94195039577508 28.974873324495125 21.45107678715767 68-69 20.71966401538162 28.941710407006994 29.000042528369306 21.338583049242075 70-71 20.77883025109006 28.729020046424164 28.977463477528353 21.514686224957416 72-73 20.707223380672122 29.023488428525013 28.832549837337183 21.436738353465685 74-75 20.656572459924814 29.21078387756918 28.517517562796346 21.615126099709663 76-77 20.655989480282173 29.020075196350003 28.671854948960757 21.652080374407063 78-79 20.545333866477673 29.04795822062571 28.695668438141524 21.711039474755093 80-81 20.633877789083265 29.08348213394154 28.490549902526425 21.792090174448763 82-83 20.831443970291964 29.032832470955572 28.47834324084254 21.65738031790992 84-85 20.79725648187546 29.11540174723401 28.323446557099995 21.763895213790533 86-87 20.663752219492928 29.207333026816805 28.374556418938656 21.754358334751608 88-89 20.68333589528799 29.02833154664118 28.338356266582814 21.949976291488017 90-91 20.74614042535041 29.014698982628108 28.38826071855825 21.850899873463227 92-93 20.675832795991003 29.01318561919607 28.284752509430994 22.026229075381938 94-95 20.610690852933164 29.060096222593394 28.243072178986665 22.086140745486777 96-97 20.560730082745682 28.908761933521284 28.20808447336569 22.322423510367344 98-99 20.41148999262353 28.922195209570834 27.978095671963864 22.68821912584177 100-101 20.55396729862574 29.142240946935498 27.904303340355103 22.39948841408366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 73988.0 1 67109.0 2 44800.5 3 22936.5 4 14141.5 5 11128.0 6 10410.0 7 10617.0 8 10976.0 9 10891.0 10 10063.0 11 8554.5 12 6851.5 13 5253.0 14 4049.5 15 3362.5 16 3144.5 17 3361.0 18 3872.0 19 4527.0 20 5227.0 21 5992.0 22 6762.5 23 7627.0 24 8744.5 25 9943.0 26 11342.5 27 12938.0 28 14589.5 29 16600.0 30 18985.0 31 21193.0 32 23420.5 33 25666.0 34 27569.5 35 29334.0 36 31157.5 37 32939.0 38 34300.0 39 35061.0 40 35706.5 41 36373.5 42 36653.5 43 36971.5 44 36944.5 45 36734.0 46 36697.5 47 35879.0 48 34598.0 49 33170.5 50 31203.0 51 28749.0 52 26287.0 53 23958.0 54 21464.0 55 18507.0 56 15264.0 57 12406.5 58 9920.0 59 7757.5 60 5989.0 61 4519.5 62 3224.0 63 2155.5 64 1453.5 65 968.5 66 623.5 67 391.0 68 228.5 69 145.5 70 96.5 71 61.5 72 45.0 73 31.0 74 20.5 75 13.5 76 10.0 77 7.5 78 4.0 79 2.0 80 1.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.949954715589858 2 28.878709915282087 3 22.70175607332067 4 29.00310178172113 5 32.09574647142273 6 24.77898803369481 7 23.680300079347905 8 19.72124039209086 9 11.653802688210826 10-11 15.242932826789135 12-13 15.32749044218423 14-15 19.470813596543955 16-17 22.726922984443 18-19 26.67624451978488 20-21 28.72706725336026 22-23 28.293338783492427 24-25 26.849327145799773 26-27 28.291655646124376 28-29 25.283849094712547 30-31 27.108009329389983 32-33 26.127341364303057 34-35 24.216299181674643 36-37 27.16844197584297 38-39 27.266584914280216 40-41 24.32337877804227 42-43 25.174685613984465 44-45 23.919465884408535 46-47 27.165756970993932 48-49 22.580490033422297 50-51 28.185457693140016 52-53 21.772664246154832 54-55 22.89102887782827 56-57 25.00785464105092 58-59 24.29444484519144 60-61 23.858632490963156 62-63 23.666915129801954 64-65 22.189240744748208 66-67 22.706044066139285 68-69 23.67328700698101 70-71 22.17124720478973 72-73 22.049580417898966 74-75 19.378481489496423 76-77 21.616052321527327 78-79 20.724189889954875 80-81 21.740644561462567 82-83 22.58097092981317 84-85 19.567754294004022 86-87 21.11876537866583 88-89 19.963892695985315 90-91 21.07736821435155 92-93 21.070074619089986 94-95 20.127637917077433 96-97 19.047304174982166 98-99 18.45403832744235 100-101 20.09906465651975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1247670.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.70993628024065 #Duplication Level Percentage of deduplicated Percentage of total 1 93.15211216866905 78.90909486177785 2 4.841714577257 8.202826666531058 3 1.0111499417164735 2.5696334139771464 4 0.389129235149339 1.3185245085711716 5 0.20373913650594305 0.8629364635604845 6 0.11618096907879749 0.5905009492584928 7 0.07295754442746763 0.4326160257729528 8 0.048203955671384795 0.32666832107028415 9 0.03332844686368226 0.254092554912772 >10 0.12621449577645927 1.6483112928722639 >50 0.0037688540598032967 0.20883315358271726 >100 0.0011255180127951164 0.13403427316290745 >500 1.8757840588665955E-4 0.1176636343695906 >1k 9.378920294332978E-5 0.10066159672266799 >5k 0.0 0.0 >10k+ 9.378920294332978E-5 4.3236022838576105 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 54420 4.3617302652143595 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1267 0.10154928787259451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 8.014939847876441E-5 14-15 0.0 0.0 0.0 0.0 8.014939847876441E-5 16-17 0.0 0.0 0.0 0.0 8.014939847876441E-5 18-19 0.0 0.0 0.0 0.0 8.014939847876441E-5 20-21 0.0 0.0 0.0 0.0 8.014939847876441E-5 22-23 0.0 0.0 0.0 8.014939847876441E-5 8.014939847876441E-5 24-25 0.0 0.0 0.0 1.6029879695752882E-4 8.014939847876441E-5 26-27 0.0 0.0 0.0 2.8052289467567546E-4 8.014939847876441E-5 28-29 0.0 0.0 0.0 5.209710901119687E-4 8.014939847876441E-5 30-31 0.0 0.0 0.0 0.001522838571096524 8.014939847876441E-5 32-33 0.0 0.0 0.0 0.003726947029262545 8.014939847876441E-5 34-35 0.0 0.0 0.0 0.006411951878301153 8.014939847876441E-5 36-37 0.0 0.0 0.0 0.01106061699006949 8.014939847876441E-5 38-39 0.0 0.0 0.0 0.01855458574783396 8.014939847876441E-5 40-41 0.0 0.0 0.0 0.026930197888864846 8.014939847876441E-5 42-43 0.0 0.0 0.0 0.034784838939783756 8.014939847876441E-5 44-45 0.0 0.0 0.0 0.04456306555419301 8.014939847876441E-5 46-47 0.0 0.0 0.0 0.05602442953665633 8.014939847876441E-5 48-49 0.0 0.0 0.0 0.06940937908260998 8.014939847876441E-5 50-51 0.0 0.0 0.0 0.08419694310194202 8.014939847876441E-5 52-53 0.0 0.0 0.0 0.10499571200718139 8.014939847876441E-5 54-55 0.0 0.0 0.0 0.12707687128808098 8.014939847876441E-5 56-57 0.0 0.0 0.0 0.1531655004929188 8.014939847876441E-5 58-59 0.0 0.0 0.0 0.1806166694718956 8.014939847876441E-5 60-61 0.0 0.0 0.0 0.2115543372846987 8.014939847876441E-5 62-63 0.0 0.0 0.0 0.24513693524730096 8.014939847876441E-5 64-65 0.0 0.0 0.0 0.2775573669319612 8.014939847876441E-5 66-67 0.0 0.0 0.0 0.3172713938781889 8.014939847876441E-5 68-69 0.0 0.0 0.0 0.36167416063542446 8.014939847876441E-5 70-71 0.0 0.0 0.0 0.4052754334078723 8.014939847876441E-5 72-73 0.0 0.0 0.0 0.4533249977958915 8.014939847876441E-5 74-75 0.0 0.0 0.0 0.5052217333108915 8.014939847876441E-5 76-77 0.0 0.0 0.0 0.5628491508171232 8.014939847876441E-5 78-79 0.0 0.0 0.0 0.6319780070050575 8.014939847876441E-5 80-81 0.0 0.0 0.0 0.7091218030408681 8.014939847876441E-5 82-83 0.0 0.0 0.0 0.7898723220082233 8.014939847876441E-5 84-85 0.0 0.0 0.0 0.8696610481938334 8.014939847876441E-5 86-87 0.0 0.0 0.0 0.9542186635889298 8.014939847876441E-5 88-89 0.0 0.0 0.0 1.0519207803345436 8.014939847876441E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1085 0.0 60.867947 1 GTATCAA 1935 0.0 38.627853 1 ATCAACG 2290 0.0 32.756294 3 TAACGCA 190 5.456968E-12 31.790228 4 TCAACGC 2385 0.0 31.44589 4 ATAACGC 210 0.0 31.165064 3 CAACGCA 2470 0.0 30.762112 5 TATCAAC 2390 0.0 30.415339 2 AACGCAG 2755 0.0 28.031319 6 TATAACG 185 4.0345185E-9 27.671362 2 GTACATG 1750 0.0 25.15875 1 ACGCAGA 3035 0.0 24.873053 7 TACATGG 1715 0.0 24.775145 2 CGCAGAG 3085 0.0 24.616245 8 ACATGGG 1805 0.0 23.986408 3 GTGGTAT 585 0.0 23.628523 1 GTAGCCC 365 0.0 22.068413 3 TGGTATC 535 0.0 22.007782 2 GCAGAGT 3295 0.0 21.265118 9 CAGAGTA 3025 0.0 20.681982 10-11 >>END_MODULE