FastQCFastQC Report
Sat 18 Jun 2016
SRR1931033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247670
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43290.34696674601457117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.2980756129425248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23100.18514511048594579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.057.361261
GTATCAA38700.043.209811
TAACGCA1950.041.4140364
TCAACGC46450.035.180824
ATCAACG46900.034.9445533
CAACGCA47200.034.5746275
TATCAAC48500.033.9892
AACGCAG49400.033.5150226
ATAACGC2500.032.3029483
TATAACG2700.031.6708282
ACGCAGA55700.029.3835987
CGCAGAG56650.028.9745888
GTATAAC4850.025.4672641
GCAGAGT64250.024.9565569
AGAGTAC56900.022.8443810-11
TCCGCAC1050.0021951622.6211133
GTACATG30300.022.263651
GTGGTAT9900.022.0736051
GAGTACT46700.021.58654412-13
TACATGG30650.021.0794412
Produced by FastQC (version 0.11.5)