##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931033_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1247670 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.342897561053803 31.0 31.0 34.0 30.0 34.0 2 31.497317399632916 31.0 31.0 34.0 30.0 34.0 3 31.558823246531535 31.0 31.0 34.0 30.0 34.0 4 35.33665712888825 37.0 35.0 37.0 33.0 37.0 5 35.22117306659614 37.0 35.0 37.0 33.0 37.0 6 35.24924699640129 37.0 35.0 37.0 32.0 37.0 7 35.13593899027788 37.0 35.0 37.0 32.0 37.0 8 35.13688555467391 37.0 35.0 37.0 32.0 37.0 9 36.6384404530044 39.0 35.0 39.0 32.0 39.0 10-11 36.57919682287785 38.0 35.0 39.0 32.0 39.0 12-13 36.47019123646477 38.0 35.0 39.0 32.0 39.0 14-15 37.51763487140029 39.0 36.0 41.0 32.0 41.0 16-17 37.470343520321876 39.0 36.0 41.0 32.0 41.0 18-19 37.49918688435243 39.0 36.0 41.0 32.0 41.0 20-21 37.490537161268605 39.0 36.0 41.0 32.0 41.0 22-23 37.38543605280242 39.0 36.0 41.0 32.0 41.0 24-25 37.2623233707631 39.0 36.0 40.5 31.0 41.0 26-27 37.00744868434762 39.0 36.0 40.0 31.0 41.0 28-29 36.81721048033535 39.0 36.0 40.0 30.5 41.0 30-31 36.57813003438409 38.0 35.0 40.0 30.0 41.0 32-33 36.392964085054544 38.0 35.0 40.0 30.0 41.0 34-35 36.449844510166955 38.0 35.0 40.0 30.0 41.0 36-37 36.50198570134731 38.0 35.0 40.0 30.0 41.0 38-39 36.48372366090393 39.0 35.0 40.0 30.0 41.0 40-41 36.364256173507414 39.0 35.0 40.0 30.0 41.0 42-43 36.2157850232834 38.0 35.0 40.0 30.0 41.0 44-45 36.04194458470589 38.0 35.0 40.0 29.5 41.0 46-47 35.78318145022322 38.0 35.0 40.0 28.5 41.0 48-49 35.54891718162655 38.0 34.5 40.0 27.5 41.0 50-51 35.28103945754887 38.0 34.0 40.0 26.5 41.0 52-53 35.0731980411487 38.0 34.0 40.0 26.0 41.0 54-55 34.9261275016631 38.0 34.0 40.0 26.0 41.0 56-57 34.7552634109981 38.0 34.0 40.0 26.0 41.0 58-59 34.544376317455736 38.0 33.0 40.0 26.0 41.0 60-61 34.241164731058696 37.5 33.0 40.0 25.0 41.0 62-63 33.98750430803017 37.0 33.0 40.0 24.0 41.0 64-65 33.67264220507025 37.0 32.0 39.5 23.0 41.0 66-67 33.34308110317632 36.0 32.0 39.0 23.0 41.0 68-69 32.99738031691072 36.0 31.5 39.0 22.0 40.0 70-71 32.60230028773634 35.5 31.0 39.0 22.0 40.0 72-73 32.171840711085466 35.0 31.0 38.0 21.0 40.0 74-75 31.647187958354372 35.0 30.5 37.0 20.0 39.5 76-77 30.27442833441535 33.5 28.5 36.0 18.5 38.5 78-79 30.998574943695047 34.0 30.0 36.0 19.5 39.0 80-81 30.95917069417394 34.0 30.0 36.0 19.5 38.5 82-83 30.677355390447794 34.0 30.0 36.0 18.0 37.0 84-85 30.327322128447427 34.0 30.0 35.0 18.0 37.0 86-87 29.973247333028766 34.0 30.0 35.0 14.5 36.5 88-89 29.618159449213334 34.0 29.0 35.0 9.0 36.0 90-91 29.276832816369712 34.0 29.0 35.0 7.0 36.0 92-93 28.946379250923723 34.0 29.0 35.0 2.0 35.0 94-95 28.55123189625462 34.0 29.0 35.0 2.0 35.0 96-97 28.116431828929123 33.5 29.0 35.0 2.0 35.0 98-99 27.610639431901063 33.0 27.5 35.0 2.0 35.0 100-101 26.472147683281637 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27125776520349376 1101 2 0.25691783935783974 1101 3 0.2006143801673801 1101 4 0.015103486003638977 1101 5 0.10784222765207119 1101 6 0.20730410509707298 1101 7 0.3437020524481369 1101 8 0.3126557300549919 1101 9 0.3119819358478466 1101 10-11 0.3644218613456687 1101 12-13 0.28089076783105327 1101 14-15 0.42296060803410285 1101 16-17 0.4986825765982772 1101 18-19 0.4560484369305655 1101 20-21 0.32753222433540685 1101 22-23 0.38548092204445794 1101 24-25 0.3345615897742462 1101 26-27 0.29047816151275896 1101 28-29 0.3975133058920335 1101 30-31 0.40813902898094767 1101 32-33 0.3705596433266649 1101 34-35 0.4128618113399014 1101 36-37 0.5461351740152907 1101 38-39 0.5252184528519095 1101 40-41 0.42470230756158855 1101 42-43 0.48294565259475064 1101 44-45 0.5381431284581382 1101 46-47 0.5432866037164032 1101 48-49 0.518313257378928 1101 50-51 0.3658327850509764 1101 52-53 0.36060754823364505 1101 54-55 0.35469828202938913 1101 56-57 0.49238289541366953 1101 58-59 0.4804963602720349 1101 60-61 0.1963431945773877 1101 62-63 0.1773921956656892 1101 64-65 0.23371449740015038 1101 66-67 0.11655760003291249 1101 68-69 0.2901001492539024 1101 70-71 0.20854058908214057 1101 72-73 0.385613642043829 1101 74-75 0.2624719414128158 1101 76-77 0.13563469804273254 1101 78-79 0.4390756685007169 1101 80-81 0.3769537737662425 1101 82-83 0.3650931732817746 1101 84-85 0.36936090647122555 1101 86-87 0.3402208581837094 1101 88-89 0.2591104916496434 1101 90-91 0.30199808867888933 1101 92-93 0.3202337569054414 1101 94-95 0.49952142608062644 1101 96-97 0.4702096927050192 1101 98-99 0.747784507013673 1101 100-101 1.0129725413196589 1102 1 0.2808479880647461 1102 2 0.30396552687281897 1102 3 0.34068147817840355 1102 4 0.07387655094493084 1102 5 0.3593255729671938 1102 6 0.3604073881068999 1102 7 0.4504501952710456 1102 8 0.44428764953737954 1102 9 0.4529404788624589 1102 10-11 0.5138515282519691 1102 12-13 0.46466383277234513 1102 14-15 0.5117360693329331 1102 16-17 0.4943391785632869 1102 18-19 0.45989219441918294 1102 20-21 0.43106804988057235 1102 22-23 0.47357222326660064 1102 24-25 0.4249342233711815 1102 26-27 0.3427247467032686 1102 28-29 0.4987713935987159 1102 30-31 0.3321816841450911 1102 32-33 0.4727139687520179 1102 34-35 0.4521105532521972 1102 36-37 0.4495116417842766 1102 38-39 0.46689230859118425 1102 40-41 0.4164971793564547 1102 42-43 0.36305828139897045 1102 44-45 0.5260355320047481 1102 46-47 0.5677366396617671 1102 48-49 0.4826626463077517 1102 50-51 0.21885818644086186 1102 52-53 0.17122101576299542 1102 54-55 0.41215215214680967 1102 56-57 0.40379674636022855 1102 58-59 0.48391596247668645 1102 60-61 0.1921004435589353 1102 62-63 -0.11183617720754313 1102 64-65 0.04649067760624348 1102 66-67 0.08351901867657574 1102 68-69 0.12757319023161529 1102 70-71 0.17950033027552337 1102 72-73 0.08749598088879651 1102 74-75 0.15979640822326502 1102 76-77 0.16217555593873456 1102 78-79 0.3716900945730863 1102 80-81 0.45780927340679156 1102 82-83 0.25105770719023823 1102 84-85 0.45285958847457763 1102 86-87 0.5078149152170219 1102 88-89 0.33191902268247375 1102 90-91 0.333646782664033 1102 92-93 0.21969218010217872 1102 94-95 0.25594725881486724 1102 96-97 0.2216606871933955 1102 98-99 0.3439958657484006 1102 100-101 0.6033463730953592 1103 1 0.4069094323587841 1103 2 0.4764915700721595 1103 3 0.352776116516349 1103 4 0.2614364355897436 1103 5 0.3509252433809422 1103 6 0.37408816028040803 1103 7 0.4482243109793629 1103 8 0.41603151393617566 1103 9 0.45183754201354276 1103 10-11 0.5195933915913784 1103 12-13 0.6273576118318474 1103 14-15 0.5610816052048548 1103 16-17 0.5971730317796968 1103 18-19 0.6063414911056597 1103 20-21 0.6549624645707084 1103 22-23 0.616756261875274 1103 24-25 0.4998562409098781 1103 26-27 0.4836052128323942 1103 28-29 0.40873254950478355 1103 30-31 0.5075018536465947 1103 32-33 0.5885882551389301 1103 34-35 0.46613444825543127 1103 36-37 0.4261063095495743 1103 38-39 0.5218016723720069 1103 40-41 0.5129132231443378 1103 42-43 0.66878955089458 1103 44-45 0.5735047804303832 1103 46-47 0.6580559263429535 1103 48-49 0.29686369392118905 1103 50-51 0.21075242456692678 1103 52-53 0.37716945905650334 1103 54-55 0.3960798197355331 1103 56-57 0.15115652506660382 1103 58-59 0.25379619318706403 1103 60-61 0.2173187944505841 1103 62-63 0.10676631823122307 1103 64-65 -0.10898310263036137 1103 66-67 -0.037174878092301356 1103 68-69 0.06945860015451188 1103 70-71 0.0352045266147627 1103 72-73 0.022175517113844023 1103 74-75 0.2115682285987539 1103 76-77 0.25953827737949453 1103 78-79 0.2528271671409179 1103 80-81 0.15471675383760797 1103 82-83 0.40121308356596685 1103 84-85 0.275281458876556 1103 86-87 0.26036538311178603 1103 88-89 0.1415691315636458 1103 90-91 0.24180757367103567 1103 92-93 0.30281824684339753 1103 94-95 0.51700576037452 1103 96-97 0.5740201716263336 1103 98-99 0.4780694929628062 1103 100-101 0.43164017569673163 1104 1 0.3988996952121582 1104 2 0.45901682824665 1104 3 0.38070814329926606 1104 4 0.2773215686250694 1104 5 0.4276387142343623 1104 6 0.29869792198899603 1104 7 0.2650105106150846 1104 8 0.21432416915943264 1104 9 0.5179720712339204 1104 10-11 0.4257499689220765 1104 12-13 0.49290161608925587 1104 14-15 0.6236269742080012 1104 16-17 0.5848121946203264 1104 18-19 0.4848194450133505 1104 20-21 0.4491012073786962 1104 22-23 0.4825421380541499 1104 24-25 0.28088460305931306 1104 26-27 0.332823871391696 1104 28-29 0.554979729347366 1104 30-31 0.5480685892520469 1104 32-33 0.7053103399768759 1104 34-35 0.7631116546376191 1104 36-37 0.7770010626874182 1104 38-39 0.8195886740192719 1104 40-41 0.7298706069838374 1104 42-43 0.7266012644144482 1104 44-45 0.9840118812018943 1104 46-47 1.0421447899139267 1104 48-49 1.0634399925485525 1104 50-51 1.0038141310237805 1104 52-53 0.9903312122733468 1104 54-55 1.00848366916032 1104 56-57 1.2823713217471635 1104 58-59 1.4126815412837104 1104 60-61 1.2785931106507036 1104 62-63 1.1732067188564557 1104 64-65 1.0615069085493687 1104 66-67 0.9757391798266468 1104 68-69 1.0746820534859154 1104 70-71 1.1210382121374494 1104 72-73 0.9302030703328796 1104 74-75 0.8768246964384367 1104 76-77 0.9545464329893356 1104 78-79 1.0781650815303685 1104 80-81 1.0602065257399893 1104 82-83 0.8000224048889173 1104 84-85 0.7666657446394396 1104 86-87 0.9398585648605184 1104 88-89 1.0766970065674641 1104 90-91 0.9606342036831066 1104 92-93 0.739493640887062 1104 94-95 1.048819845911268 1104 96-97 1.1206717590153197 1104 98-99 1.1178492655513104 1104 100-101 1.3941376315731269 1105 1 0.2635657823186861 1105 2 0.2685484740461277 1105 3 0.21209018994856166 1105 4 0.14508368718218634 1105 5 0.2991471670357697 1105 6 0.2922771749465696 1105 7 0.3603621507854129 1105 8 0.36241786269771126 1105 9 0.38534548349637987 1105 10-11 0.4317871167598426 1105 12-13 0.34648843888912495 1105 14-15 0.4209818988046621 1105 16-17 0.35285979950860735 1105 18-19 0.347796503965057 1105 20-21 0.29629395349503085 1105 22-23 0.2812274595965363 1105 24-25 0.5018862622961109 1105 26-27 0.41804332872366246 1105 28-29 0.4839196790482063 1105 30-31 0.25392403817659925 1105 32-33 0.4823455720884411 1105 34-35 0.403779923039032 1105 36-37 0.38033763807714394 1105 38-39 0.307303569017499 1105 40-41 0.04606274932865517 1105 42-43 0.2379551766723651 1105 44-45 0.28181055517435283 1105 46-47 0.19852672307233377 1105 48-49 0.28698725242453094 1105 50-51 0.25923700942509953 1105 52-53 0.049006001861236825 1105 54-55 0.17079209653976335 1105 56-57 0.2435534655446645 1105 58-59 0.5633089189178335 1105 60-61 0.35952630083419734 1105 62-63 0.08534292381192188 1105 64-65 0.29700504275915307 1105 66-67 0.5195222624207787 1105 68-69 0.4722094274883304 1105 70-71 0.3595617297194593 1105 72-73 0.32319222741335096 1105 74-75 0.36134993000269233 1105 76-77 0.4611306069118548 1105 78-79 0.45420863025427494 1105 80-81 0.4306498665485883 1105 82-83 0.2747868545489034 1105 84-85 0.48118906020766516 1105 86-87 0.3947379921400973 1105 88-89 0.48278556577793097 1105 90-91 0.34408700509220935 1105 92-93 0.39294750911052034 1105 94-95 0.5253853959789119 1105 96-97 0.4205578141628159 1105 98-99 0.2822013801135519 1105 100-101 0.6397319152640328 1106 1 0.18789743447365126 1106 2 0.2668540550740808 1106 3 0.4100977439021314 1106 4 0.22607816244571666 1106 5 0.19026117090353978 1106 6 0.3017122399995458 1106 7 0.3057038037575097 1106 8 0.1879488865671206 1106 9 0.44788917748862644 1106 10-11 0.4506180925091172 1106 12-13 0.4288473583650614 1106 14-15 0.49853277677017616 1106 16-17 0.365143769601481 1106 18-19 0.42566345405629846 1106 20-21 0.3522662412897688 1106 22-23 0.4595579632237019 1106 24-25 0.5585786497812464 1106 26-27 0.49748099271798907 1106 28-29 0.5459367753157238 1106 30-31 0.4753481672475246 1106 32-33 0.7173676120190748 1106 34-35 0.8818004263030375 1106 36-37 0.5577094274199013 1106 38-39 0.599166669497798 1106 40-41 0.42504769816696353 1106 42-43 0.6576487399231112 1106 44-45 0.6023238631159913 1106 46-47 0.4068772691904954 1106 48-49 0.34791625479422095 1106 50-51 0.2370469870900962 1106 52-53 0.4164804210148674 1106 54-55 0.7835401876230108 1106 56-57 0.6550163858014244 1106 58-59 0.6319731765512344 1106 60-61 0.5084348589551482 1106 62-63 0.294156229029781 1106 64-65 0.41568927478900264 1106 66-67 0.3721219982139914 1106 68-69 0.49630978521161495 1106 70-71 0.41041274115025317 1106 72-73 0.49161979979783865 1106 74-75 0.6319113580812434 1106 76-77 0.46924123329077005 1106 78-79 0.4372457145371449 1106 80-81 0.17851850765407917 1106 82-83 0.4740992699981881 1106 84-85 0.5157237702611432 1106 86-87 0.6599037796306781 1106 88-89 0.6113616636887933 1106 90-91 0.35324912455725865 1106 92-93 0.2952585240822394 1106 94-95 0.5898899288050146 1106 96-97 0.650061359618185 1106 98-99 0.7022501698467103 1106 100-101 0.8560818666899301 1107 1 0.23819815855541293 1107 2 0.26760593605041905 1107 3 0.26523213413402047 1107 4 0.19283800271630014 1107 5 0.1297070097122628 1107 6 0.2618030998331946 1107 7 0.214302706396289 1107 8 0.20881981159020313 1107 9 0.2741610764466955 1107 10-11 0.2436082178433665 1107 12-13 0.2507928277443199 1107 14-15 0.23748825103552207 1107 16-17 0.2834364449332796 1107 18-19 0.354567106284172 1107 20-21 0.43804776438094706 1107 22-23 0.3338692815801849 1107 24-25 0.2755959583304346 1107 26-27 0.3911903307060456 1107 28-29 0.2834012265756556 1107 30-31 0.4304909368758345 1107 32-33 0.404116615691855 1107 34-35 0.5987463967475506 1107 36-37 0.5594382441075751 1107 38-39 0.43509695518425673 1107 40-41 0.48728056952001 1107 42-43 0.5070760817804043 1107 44-45 0.4846929902489805 1107 46-47 0.48557140819901434 1107 48-49 0.534501633766574 1107 50-51 0.30306757209652346 1107 52-53 0.4412556918307331 1107 54-55 0.539048933051852 1107 56-57 0.46929810450621545 1107 58-59 0.554404028130044 1107 60-61 0.4641055871756592 1107 62-63 0.291364134877071 1107 64-65 0.3777849618344078 1107 66-67 0.4374790256117933 1107 68-69 0.24760873320103371 1107 70-71 0.1687744445909587 1107 72-73 0.35361283923705145 1107 74-75 0.5010155609713429 1107 76-77 0.6600123472465995 1107 78-79 0.812232197098659 1107 80-81 0.7786851330843021 1107 82-83 0.7830793219391232 1107 84-85 0.590894033368528 1107 86-87 0.7432541454083186 1107 88-89 0.9152713315306507 1107 90-91 0.8519950459709769 1107 92-93 0.6832554350290714 1107 94-95 0.9271190209370452 1107 96-97 0.9174371421434095 1107 98-99 0.919906690460877 1107 100-101 1.048605576701668 1108 1 -0.03686539444315784 1108 2 -0.0042665302633473345 1108 3 -0.024908031246720697 1108 4 -0.09942517016500574 1108 5 -0.13607660476375116 1108 6 -5.046830791428647E-4 1108 7 0.004621837935843587 1108 8 0.1742255959136827 1108 9 0.21406446902754794 1108 10-11 0.08463480889826513 1108 12-13 0.09920077224079193 1108 14-15 0.21682680812229904 1108 16-17 0.05850028382027972 1108 18-19 0.08190015601618228 1108 20-21 0.24024613207235745 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2316 96-97 -1.1394552952697374 2316 98-99 -1.5028534766190766 2316 100-101 -1.4872235750911749 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 16.0 5 28.0 6 55.0 7 165.0 8 438.0 9 863.0 10 1447.0 11 2514.0 12 3418.0 13 4769.0 14 5522.0 15 6890.0 16 8343.0 17 9934.0 18 8630.0 19 6776.0 20 6374.0 21 6802.0 22 7613.0 23 8600.0 24 10361.0 25 13436.0 26 16775.0 27 21726.0 28 27865.0 29 34450.0 30 42537.0 31 51097.0 32 62174.0 33 74972.0 34 93035.0 35 116746.0 36 150932.0 37 207835.0 38 198585.0 39 35931.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.5813341156272 21.688215816923996 15.031183548103458 22.699266519345343 2 15.188194236265964 22.339479029235473 40.52392044206689 21.94840629243167 3 18.104363103577622 27.248558082273465 31.608054861803524 23.03902395234538 4 12.421516958760073 18.803332337742837 39.80218259129725 28.972968112199837 5 11.862749419907106 38.68287854317534 36.38118214716526 13.073189889752292 6 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20.412115227472107 28-29 20.50256238448431 28.367848446619526 30.92005392468818 20.209535244207984 30-31 20.766502737080376 28.109789405721518 30.938165909672943 20.185541947525167 32-33 20.5085572572845 28.24870940679009 30.836068708180232 20.406664627745176 34-35 20.59979855477278 28.33688247278225 30.878835866228506 20.184483106216465 36-37 20.696490058157053 28.18477208461307 30.83658476374898 20.282153093480897 38-39 20.513848073740977 28.37253123736389 30.78784400774407 20.325776681151062 40-41 20.698940244268005 28.26046009845741 30.77082762257884 20.269772034695738 42-43 20.698016585699396 28.35873238155205 30.442906549431452 20.500344483317107 44-45 20.5158253201114 28.60380570763967 30.501569646998757 20.378799325250178 46-47 20.69265419112658 28.53676591709488 30.14297400192139 20.627605889857154 48-49 20.7224661481765 28.688230567139534 29.92511217142639 20.664191113257573 50-51 20.789493231509866 28.858721946851844 29.705832163929358 20.645952657708932 52-53 20.899650064314024 28.85637390880809 29.59062900959615 20.653347017281735 54-55 20.82574282744791 28.7634379510464 29.801398296439967 20.609420925065724 56-57 20.819144224931016 28.951111449543415 29.59255199555941 20.637192329966155 58-59 20.782186857307998 28.906354902466134 29.78780116214146 20.523657078084412 60-61 20.83781553100049 28.977232375182915 29.72659028908219 20.458361804734405 62-63 20.864233832320657 28.915877534001595 29.754418861736887 20.46546977194086 64-65 20.700904713258403 28.713809561494667 29.796565054310513 20.788720670936414 66-67 20.66013437152606 28.8736471347319 29.69760982921727 20.76860866452477 68-69 20.725108007716617 28.92200384545235 29.66678906335997 20.686099083471063 70-71 20.735790924026784 28.73939014151834 29.70760374864929 20.817215185805583 72-73 20.685512390185472 28.99697581049804 29.499254589871892 20.81825720944459 74-75 20.713441832429176 29.040265220012056 29.38702029334941 20.859272654209363 76-77 20.599720079759194 29.058813371111185 29.396243767557767 20.94522278157185 78-79 20.60601299939003 29.02912559103621 29.412903365429706 20.95195804414406 80-81 20.697797252316118 29.108200973174775 29.229587334461304 20.9644144400478 82-83 20.90825298356136 29.08277028380902 29.264949866551255 20.744026866078368 84-85 20.79087418948921 29.2045572947975 29.13478724342174 20.86978127229155 86-87 20.69529603180328 29.268115767791166 29.147611147178342 20.888977053227215 88-89 20.672670395754004 29.11469307965494 29.14679293947353 21.065843585117527 90-91 20.748172271753393 29.042183030121343 29.29090315945902 20.91874153866624 92-93 20.761817649084175 29.123045122169717 29.187658251060483 20.927478977685624 94-95 20.606805775583574 29.030687346383928 29.2067810086552 21.155725869377296 96-97 20.53401182525173 28.982326241976036 29.266175564192103 21.21748636858013 98-99 20.528505133568974 28.951124896807652 29.106734953954174 21.413635015669207 100-101 20.483381022225426 29.051952840093936 29.13510784101565 21.32955829666498 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 370.0 1 279.5 2 370.0 3 556.5 4 406.0 5 286.5 6 466.0 7 917.5 8 1469.5 9 1721.5 10 1452.5 11 1123.0 12 1070.0 13 1182.5 14 1338.0 15 1453.5 16 1615.5 17 1811.5 18 1985.0 19 2173.0 20 2475.0 21 2934.0 22 3504.5 23 4347.5 24 5511.0 25 7073.5 26 9235.5 27 11541.0 28 14222.5 29 17625.5 30 21363.5 31 25050.5 32 29087.5 33 32562.0 34 35202.0 35 37695.0 36 41063.0 37 44586.0 38 46485.5 39 47882.5 40 49149.5 41 50062.0 42 50406.0 43 51027.5 44 51889.5 45 52166.0 46 51716.5 47 50991.0 48 49848.5 49 47714.0 50 44789.5 51 40966.5 52 37065.0 53 33326.0 54 29211.0 55 24395.0 56 19376.0 57 14898.0 58 11443.5 59 8609.0 60 6036.5 61 4261.5 62 3011.5 63 1846.5 64 994.5 65 581.0 66 288.5 67 136.0 68 72.5 69 31.0 70 19.5 71 14.5 72 4.5 73 3.5 74 2.5 75 1.5 76 1.0 77 1.0 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004568515713289572 2 0.006091354284386096 3 0.002083884360447875 4 0.17456538988674888 5 0.0020037349619691103 6 6.411951878301153E-4 7 8.014939847876441E-5 8 5.610457893513509E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.2059759391505764E-4 24-25 4.808963908725865E-4 26-27 0.002685004849038608 28-29 3.2059759391505764E-4 30-31 0.0065321759760192995 32-33 0.02232160747633589 34-35 0.014787564019332034 36-37 0.0011220915787027018 38-39 4.408216916332043E-4 40-41 0.001923585563490346 42-43 0.01198233507257528 44-45 0.007734416953200767 46-47 0.010860243493872579 48-49 0.011020542290830108 50-51 0.1905551948832624 52-53 0.11549528320789952 54-55 0.017913390560003847 56-57 0.007013072366891886 58-59 0.018554585747833963 60-61 0.12314955076262153 62-63 0.23636057611387626 64-65 0.2421714075035867 66-67 0.2513084389301658 68-69 0.24710059551003072 70-71 0.23684147250474882 72-73 0.16406581868603076 74-75 0.2741109427973743 76-77 0.21419926743449788 78-79 0.005850906088949803 80-81 8.014939847876441E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 8.014939847876441E-5 90-91 0.006652400073737447 92-93 0.019676677326536662 94-95 0.00308575184143243 96-97 8.014939847876441E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1247670.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.42648929972428 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66326387799093 58.216834689836126 2 11.343654063078183 15.97787462986299 3 2.7673539415626927 5.846850682620444 4 1.1315883733007814 3.1877518585583955 5 0.6039176187971281 2.1265898859065455 6 0.34778031488069416 1.4695767974759966 7 0.2457581425766475 1.2115518238946066 8 0.16517077484763362 0.9305918245947229 9 0.11767410205098812 0.7458636500053719 >10 0.5776022982106364 6.957928831755747 >50 0.02563102997786442 1.2008897449950606 >100 0.009807215031745682 1.0697572213669508 >500 4.561415395178638E-4 0.22607579753605272 >1k 3.4210615463839785E-4 0.8318625615909179 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4329 0.34696674601457117 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3719 0.2980756129425248 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2310 0.18514511048594579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.014939847876441E-5 0.0 24-25 0.0 0.0 0.0 1.6029879695752882E-4 0.0 26-27 0.0 0.0 0.0 3.606722931544399E-4 0.0 28-29 0.0 0.0 0.0 7.61419285548262E-4 0.0 30-31 0.0 0.0 0.0 0.0028453036459961368 0.0 32-33 0.0 0.0 0.0 0.007333669960806944 0.0 34-35 0.0 0.0 0.0 0.013024277252799218 0.0 36-37 0.0 0.0 0.0 0.021640337589266395 0.0 38-39 0.0 0.0 0.0 0.03646797630783781 0.0 40-41 0.0 0.0 0.0 0.054461516266320426 0.0 42-43 0.0 0.0 0.0 0.07217453333012736 0.0 44-45 0.0 0.0 0.0 0.09313360103232425 0.0 46-47 0.0 0.0 0.0 0.11685782298203852 0.0 48-49 0.0 0.0 0.0 0.14491011244960605 0.0 50-51 0.0 0.0 0.0 0.1756874814654516 0.0 52-53 0.0 0.0 0.0 0.2113138890892624 0.0 54-55 0.0 0.0 0.0 0.2501462726522238 0.0 56-57 0.0 0.0 0.0 0.293266649033799 0.0 58-59 0.0 0.0 0.0 0.34135628812105767 0.0 60-61 0.0 0.0 0.0 0.39750094175543216 0.0 62-63 0.0 0.0 0.0 0.4572122436221116 0.0 64-65 0.0 0.0 0.0 0.5162022009024823 0.0 66-67 8.014939847876441E-5 0.0 0.0 0.5869741197592312 0.0 68-69 8.014939847876441E-5 0.0 0.0 0.6664021736516867 0.0 70-71 8.014939847876441E-5 0.0 0.0 0.7487957552878566 0.0 72-73 8.014939847876441E-5 0.0 0.0 0.8383627080878758 8.014939847876441E-5 74-75 8.014939847876441E-5 0.0 0.0 0.9336202681798873 8.014939847876441E-5 76-77 8.014939847876441E-5 0.0 0.0 1.0392171006756594 8.014939847876441E-5 78-79 8.014939847876441E-5 0.0 0.0 1.1657730008736285 8.014939847876441E-5 80-81 8.014939847876441E-5 0.0 0.0 1.3085591542635473 8.014939847876441E-5 82-83 8.014939847876441E-5 0.0 0.0 1.4580778571256823 8.014939847876441E-5 84-85 8.014939847876441E-5 0.0 0.0 1.6126860467912187 8.014939847876441E-5 86-87 8.014939847876441E-5 0.0 0.0 1.7803185137095547 8.014939847876441E-5 88-89 8.014939847876441E-5 0.0 0.0 1.9741598339304463 8.014939847876441E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1880 0.0 57.36126 1 GTATCAA 3870 0.0 43.20981 1 TAACGCA 195 0.0 41.414036 4 TCAACGC 4645 0.0 35.18082 4 ATCAACG 4690 0.0 34.944553 3 CAACGCA 4720 0.0 34.574627 5 TATCAAC 4850 0.0 33.989 2 AACGCAG 4940 0.0 33.515022 6 ATAACGC 250 0.0 32.302948 3 TATAACG 270 0.0 31.670828 2 ACGCAGA 5570 0.0 29.383598 7 CGCAGAG 5665 0.0 28.974588 8 GTATAAC 485 0.0 25.467264 1 GCAGAGT 6425 0.0 24.956556 9 AGAGTAC 5690 0.0 22.84438 10-11 TCCGCAC 105 0.00219516 22.621113 3 GTACATG 3030 0.0 22.26365 1 GTGGTAT 990 0.0 22.073605 1 GAGTACT 4670 0.0 21.586544 12-13 TACATGG 3065 0.0 21.079441 2 >>END_MODULE