FastQCFastQC Report
Sat 18 Jun 2016
SRR1931032_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931032_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439814
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN638134.432030803978847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.060.1323661
GTATCAA22150.043.6512261
ATCAACG24750.036.1716233
TCAACGC24900.035.1439484
CAACGCA25650.033.9155
TATCAAC28350.032.2987252
AACGCAG27600.031.5229516
ACGCAGA32300.027.1671737
CGCAGAG32400.026.5852648
GTACATG24600.025.3707371
GTGGTAT6500.025.1851751
TACATGG23800.023.8578592
TAGTCGA1302.000132E-423.2081745
GTATTGA3600.022.7366161
GCAGAGT34750.022.6940889
ACATGGG23950.022.2599163
GCGTCGG1202.95686E-421.9061019
CAGTCCG3400.021.90619
GGCGTCG1050.003249366321.142928
TGGTATC6650.020.000332
Produced by FastQC (version 0.11.5)