FastQCFastQC Report
Sat 18 Jun 2016
SRR1931032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439814
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37000.2569776373892739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31990.22218147621845602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.1282110050325945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.063.062171
GTATCAA38800.038.7033581
ATCAACG48000.030.3941823
TCAACGC48800.029.9922524
CAACGCA48700.029.9073665
TATCAAC51700.028.7704812
AACGCAG51200.028.724036
CGCAGAG57350.025.1474428
ACGCAGA57700.025.0771227
TGGTATC10100.023.0551532
GTGGTAT11100.022.2624861
GCAGAGT66150.021.586159
GAGTACT45450.021.24071312-13
GTACATG36200.021.1354061
AGAGTAC60200.020.8433610-11
CAGAGTA63650.020.79430410-11
GTGATCG1951.0440883E-519.4629678
ACATGGG35900.019.4588073
GTACTTT50100.019.45864914-15
TACATGG38300.019.3561572
Produced by FastQC (version 0.11.5)