##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931032_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1439814 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.374495594569854 31.0 31.0 34.0 30.0 34.0 2 31.527102806334707 31.0 31.0 34.0 30.0 34.0 3 31.599863593491936 31.0 31.0 34.0 30.0 34.0 4 35.369543566043944 37.0 35.0 37.0 33.0 37.0 5 35.24950722801695 37.0 35.0 37.0 33.0 37.0 6 35.279236762526274 37.0 35.0 37.0 32.0 37.0 7 35.160873557278926 37.0 35.0 37.0 32.0 37.0 8 35.16009845716183 37.0 35.0 37.0 32.0 37.0 9 36.668724571368244 39.0 35.0 39.0 32.0 39.0 10-11 36.609244666324955 39.0 35.0 39.0 32.0 39.0 12-13 36.50605668509961 38.0 35.0 39.0 32.0 39.0 14-15 37.573975180127434 39.0 36.0 41.0 32.0 41.0 16-17 37.535325743464085 39.0 36.0 41.0 32.0 41.0 18-19 37.5527995282724 39.0 36.0 41.0 32.0 41.0 20-21 37.53758714667311 39.0 36.0 41.0 32.0 41.0 22-23 37.44290755611489 39.0 36.0 41.0 32.0 41.0 24-25 37.33112992372626 39.0 36.0 41.0 31.5 41.0 26-27 37.089826880416496 39.0 36.0 40.0 31.0 41.0 28-29 36.901086529232245 39.0 36.0 40.0 30.5 41.0 30-31 36.67101410321055 38.5 35.5 40.0 30.0 41.0 32-33 36.507963181355365 38.0 35.0 40.0 30.0 41.0 34-35 36.59143854692342 38.5 35.0 40.0 30.0 41.0 36-37 36.66566931561994 39.0 35.0 40.0 30.0 41.0 38-39 36.65162166779876 39.0 35.0 40.0 30.0 41.0 40-41 36.54603198746504 39.0 35.0 40.0 30.0 41.0 42-43 36.4097987656739 39.0 35.0 40.0 30.0 41.0 44-45 36.25365290238878 39.0 35.0 40.0 30.0 41.0 46-47 36.01981297584271 38.0 35.0 40.0 29.5 41.0 48-49 35.809167711940574 38.0 35.0 40.0 28.5 41.0 50-51 35.55652084227546 38.0 34.5 40.0 27.5 41.0 52-53 35.35947490439737 38.0 34.0 40.0 27.0 41.0 54-55 35.21961100531041 38.0 34.0 40.0 27.0 41.0 56-57 35.05998969311314 38.0 34.0 40.0 26.5 41.0 58-59 34.86832708947128 38.0 34.0 40.0 26.0 41.0 60-61 34.57943248225118 38.0 33.0 40.0 26.0 41.0 62-63 34.336236486101676 37.0 33.0 40.0 26.0 41.0 64-65 34.0363960205971 37.0 33.0 40.0 25.0 41.0 66-67 33.72955569261029 36.5 32.0 39.0 24.5 41.0 68-69 33.396497047535306 36.0 32.0 39.0 23.5 40.5 70-71 33.025683178521675 36.0 31.5 39.0 23.0 40.0 72-73 32.60358803289869 35.0 31.0 38.5 22.0 40.0 74-75 32.08462238872521 35.0 31.0 37.5 21.0 39.5 76-77 30.700275868966408 33.5 29.5 36.0 20.0 39.0 78-79 31.431268900010696 34.0 30.5 36.0 21.0 39.0 80-81 31.392135025774163 34.0 31.0 36.0 21.0 39.0 82-83 31.106799558831902 34.0 31.0 36.0 20.5 37.5 84-85 30.755799707462216 34.0 30.0 35.5 20.0 37.0 86-87 30.404213669265612 34.0 30.0 35.0 18.5 36.5 88-89 30.047374869253943 34.0 30.0 35.0 18.0 36.0 90-91 29.707264618902165 34.0 29.5 35.0 13.0 36.0 92-93 29.384000989016634 34.0 29.0 35.0 7.0 35.5 94-95 28.99822893790448 34.0 29.0 35.0 2.0 35.0 96-97 28.576047322779193 34.0 29.0 35.0 2.0 35.0 98-99 28.0946861886327 34.0 29.0 35.0 2.0 35.0 100-101 26.9522500823023 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25648931641026707 1101 2 0.3865423698810204 1101 3 0.2890935514592847 1101 4 0.12067903268003732 1101 5 0.1309738504808493 1101 6 0.2465004116308407 1101 7 0.34384074642840545 1101 8 0.29546309093731793 1101 9 0.45206025936219163 1101 10-11 0.3770645705921467 1101 12-13 0.3574647440110752 1101 14-15 0.3862232577538194 1101 16-17 0.5236258331902945 1101 18-19 0.5579310424834389 1101 20-21 0.3104931246329059 1101 22-23 0.4350659255913101 1101 24-25 0.4065094366276867 1101 26-27 0.506242645304809 1101 28-29 0.48124226449289864 1101 30-31 0.6666059626522411 1101 32-33 0.5794288296183296 1101 34-35 0.4842006061627373 1101 36-37 0.6192685229564319 1101 38-39 0.7318838506027845 1101 40-41 0.7721601284645487 1101 42-43 0.5727191574627852 1101 44-45 0.7537094460035405 1101 46-47 0.6451385020077183 1101 48-49 0.6375683660457412 1101 50-51 0.5421191725825523 1101 52-53 0.3707584740799419 1101 54-55 0.4078598666659232 1101 56-57 0.7150550180650797 1101 58-59 0.5888539133676929 1101 60-61 0.38854097444988867 1101 62-63 0.1546533656421687 1101 64-65 0.3876543926788969 1101 66-67 0.31122560608122285 1101 68-69 0.3754239518830005 1101 70-71 0.4461026947868447 1101 72-73 0.4421308800943109 1101 74-75 0.4657003382350666 1101 76-77 0.20062802088250464 1101 78-79 0.3456950269860748 1101 80-81 0.31806834246018667 1101 82-83 0.3175567800167798 1101 84-85 0.3118329792170762 1101 86-87 0.38142047533373713 1101 88-89 0.33632642458932693 1101 90-91 0.4400203119660695 1101 92-93 0.30211986235663346 1101 94-95 0.48653736527102254 1101 96-97 0.5569139244172732 1101 98-99 0.5999767087938217 1101 100-101 0.8418663793959134 1102 1 0.29286206665369363 1102 2 0.33193674512921234 1102 3 0.37445294056893985 1102 4 0.1970891498305889 1102 5 0.2608294326857248 1102 6 0.23931765137145078 1102 7 0.36817945540083485 1102 8 0.27469599985524695 1102 9 0.538524008905334 1102 10-11 0.49829870151396705 1102 12-13 0.49922672667052126 1102 14-15 0.621953525971584 1102 16-17 0.6233309805793965 1102 18-19 0.6265943618142629 1102 20-21 0.5935281489009299 1102 22-23 0.6552149468257227 1102 24-25 0.5236978884120091 1102 26-27 0.5098363504038446 1102 28-29 0.6291807606841431 1102 30-31 0.4908217737456937 1102 32-33 0.6905033095459387 1102 34-35 0.6699193890319961 1102 36-37 0.7320027984913366 1102 38-39 0.6598230535846952 1102 40-41 0.5980292252511603 1102 42-43 0.6301882144459228 1102 44-45 0.7066808963101181 1102 46-47 0.7901719490165533 1102 48-49 0.7265414549907021 1102 50-51 0.4571922277689566 1102 52-53 0.45915566812094966 1102 54-55 0.49841509724242883 1102 56-57 0.3888476382404207 1102 58-59 0.575377693619032 1102 60-61 0.4254087106644491 1102 62-63 0.2412738181184082 1102 64-65 0.31217100830473754 1102 66-67 0.48235636886646205 1102 68-69 0.5916102953851237 1102 70-71 0.662837033560919 1102 72-73 0.46917724835271457 1102 74-75 0.47892964624049483 1102 76-77 0.6237554673314847 1102 78-79 0.7221687087982112 1102 80-81 0.7599176044684235 1102 82-83 0.6976399767194188 1102 84-85 0.739431574191606 1102 86-87 0.8336372405946015 1102 88-89 0.7790480150915471 1102 90-91 0.8669632655885131 1102 92-93 0.6991258133481395 1102 94-95 0.7660828430661688 1102 96-97 0.9712990211778489 1102 98-99 1.0957364297231038 1102 100-101 1.4948379692006455 1103 1 0.3427883684494404 1103 2 0.34501714707949205 1103 3 0.2910568577817507 1103 4 0.33187634912498964 1103 5 0.27261336839856654 1103 6 0.3306947327395733 1103 7 0.41302511004308684 1103 8 0.39284872302905427 1103 9 0.45982567198697666 1103 10-11 0.3326401433271471 1103 12-13 0.4804798889227655 1103 14-15 0.4381762345338416 1103 16-17 0.504505549712519 1103 18-19 0.5245093110672627 1103 20-21 0.5392927208795086 1103 22-23 0.5397182868045505 1103 24-25 0.6150578147046559 1103 26-27 0.605079728315971 1103 28-29 0.6058532890494845 1103 30-31 0.5770109315550087 1103 32-33 0.6214099850204065 1103 34-35 0.8060476577600824 1103 36-37 0.6869505967403242 1103 38-39 0.73434630260045 1103 40-41 0.9487141931048342 1103 42-43 1.0121224103211972 1103 44-45 0.8904510969833268 1103 46-47 0.9873144166575045 1103 48-49 0.9469642749923324 1103 50-51 0.9191308336423063 1103 52-53 1.0540868236593823 1103 54-55 0.9729748065119352 1103 56-57 0.993121447884036 1103 58-59 1.0051166802893121 1103 60-61 0.9309205220482824 1103 62-63 0.7056214540575141 1103 64-65 0.6882477752088931 1103 66-67 0.8226179128598545 1103 68-69 0.7170651336196272 1103 70-71 0.7482242487752302 1103 72-73 0.6345018544035597 1103 74-75 0.8470219835486148 1103 76-77 0.6743325183236877 1103 78-79 0.926460725695275 1103 80-81 0.7099478431481323 1103 82-83 0.6100640026084854 1103 84-85 0.4543471574961977 1103 86-87 0.4365169144377319 1103 88-89 0.46971455610681545 1103 90-91 0.5359759841327367 1103 92-93 0.601040182530852 1103 94-95 0.7517970111456513 1103 96-97 0.9306737438374597 1103 98-99 0.6313993155636233 1103 100-101 1.0297073756167343 1104 1 0.2751773432331355 1104 2 0.27423562492070985 1104 3 0.38468392763409653 1104 4 0.1694840116930223 1104 5 0.2831816915401646 1104 6 0.2637615747146782 1104 7 0.2844251637742943 1104 8 0.3197898517757878 1104 9 0.4455304136768774 1104 10-11 0.3408881946813693 1104 12-13 0.44607768416418025 1104 14-15 0.40249791365179277 1104 16-17 0.4144361706281927 1104 18-19 0.411227206227359 1104 20-21 0.313287173399452 1104 22-23 0.36191203227875945 1104 24-25 0.33005558183503325 1104 26-27 0.33007447973798065 1104 28-29 0.3891137056976177 1104 30-31 0.37108986838880753 1104 32-33 0.4033406877783108 1104 34-35 0.3216993176451055 1104 36-37 0.49093555734040706 1104 38-39 0.4905732066333215 1104 40-41 0.36890022405899003 1104 42-43 0.40761076826353815 1104 44-45 0.4817329865425677 1104 46-47 0.32459849104853333 1104 48-49 0.3990169582611074 1104 50-51 0.4065698300430327 1104 52-53 0.43298166884412836 1104 54-55 0.4113017911416037 1104 56-57 0.4894265379371845 1104 58-59 0.5719126191353681 1104 60-61 0.5140050668542386 1104 62-63 0.16855737514736546 1104 64-65 0.21127079600570653 1104 66-67 0.33031375153814224 1104 68-69 0.30504331021025877 1104 70-71 0.5164670604473542 1104 72-73 0.43783808186802986 1104 74-75 0.5062676205569687 1104 76-77 0.48217251497883495 1104 78-79 0.7806583426629494 1104 80-81 0.4669377925660747 1104 82-83 0.3925053297336696 1104 84-85 0.4267836719422391 1104 86-87 0.5381200436577238 1104 88-89 0.7013476467008317 1104 90-91 0.6811765598750554 1104 92-93 0.4473769419698428 1104 94-95 0.38303481264976824 1104 96-97 0.40127659194717324 1104 98-99 0.582858865039924 1104 100-101 1.0049432057905427 1105 1 0.25102871898535284 1105 2 0.35275556061312585 1105 3 0.3625440620519633 1105 4 0.09108354338088986 1105 5 0.23935764309785412 1105 6 0.2600794193866065 1105 7 0.22914248688577032 1105 8 0.21891586255515705 1105 9 0.398370665309308 1105 10-11 0.4324040480027307 1105 12-13 0.4326597906812921 1105 14-15 0.4409484899965719 1105 16-17 0.4123058112361804 1105 18-19 0.4463634161076513 1105 20-21 0.4958896508115842 1105 22-23 0.449359594632206 1105 24-25 0.47447031113901517 1105 26-27 0.43251903834014627 1105 28-29 0.5167893483467907 1105 30-31 0.40902886834443564 1105 32-33 0.5748996858907276 1105 34-35 0.5434806957525424 1105 36-37 0.5133745180424683 1105 38-39 0.46457326466033066 1105 40-41 0.33316923729256587 1105 42-43 0.4072045791705676 1105 44-45 0.5732718800015562 1105 46-47 0.3327969400206001 1105 48-49 0.22951450576645271 1105 50-51 0.4513316974205779 1105 52-53 0.3390154542192718 1105 54-55 0.3258254405628733 1105 56-57 0.41155428611359923 1105 58-59 0.3809555318183655 1105 60-61 0.5094269061740206 1105 62-63 0.27104118731732996 1105 64-65 0.16810552483182306 1105 66-67 0.32153033907188444 1105 68-69 0.25195712644211454 1105 70-71 0.38342818576181514 1105 72-73 0.21588560940526946 1105 74-75 0.19855932917943875 1105 76-77 0.3809004433803267 1105 78-79 0.43382491091125175 1105 80-81 0.34152093674272166 1105 82-83 0.3438227989366851 1105 84-85 0.29587302330262943 1105 86-87 0.3443516328810823 1105 88-89 0.42236486024365405 1105 90-91 0.326967724067611 1105 92-93 0.20773647051742472 1105 94-95 0.2832321898287695 1105 96-97 0.18735446078564166 1105 98-99 0.2199954708692502 1105 100-101 0.1961255744532231 1106 1 0.15019018752548163 1106 2 0.19752733403884903 1106 3 0.0889573760871194 1106 4 0.10845446546662885 1106 5 0.1442543423408651 1106 6 0.08984942096632409 1106 7 0.26027702336658365 1106 8 0.06465126403838184 1106 9 0.22909276727371264 1106 10-11 0.1672361922779615 1106 12-13 0.07078052384128597 1106 14-15 0.21104698755654994 1106 16-17 0.2001962044152208 1106 18-19 0.3365996907053628 1106 20-21 0.10260943016339041 1106 22-23 -0.011821607575988935 1106 24-25 0.21232697520127886 1106 26-27 0.3669748254453822 1106 28-29 0.2985331436836347 1106 30-31 0.5074011182473015 1106 32-33 0.4728955786235929 1106 34-35 0.5321861380949642 1106 36-37 0.23260353020629765 1106 38-39 0.43156761610065075 1106 40-41 0.35528614497297895 1106 42-43 0.4302428467748243 1106 44-45 0.4079481872183095 1106 46-47 0.5673889244454386 1106 48-49 0.5073899129708224 1106 50-51 0.28442017444422163 1106 52-53 0.2895993095000122 1106 54-55 0.3572456071590935 1106 56-57 0.4963768239948152 1106 58-59 0.6107953398612622 1106 60-61 0.7602554042890333 1106 62-63 0.4338832283131566 1106 64-65 0.4774145475840328 1106 66-67 0.433945856173402 1106 68-69 0.3638897621377666 1106 70-71 0.5174610780549216 1106 72-73 0.6790952550787566 1106 74-75 0.7604198101822632 1106 76-77 0.5657603394604891 1106 78-79 0.5728520607154941 1106 80-81 0.3952082977168523 1106 82-83 0.4947883482729907 1106 84-85 0.6493334599025644 1106 86-87 0.4446671495556451 1106 88-89 0.5064479192610705 1106 90-91 0.4490666998125299 1106 92-93 0.49080893688437044 1106 94-95 0.7195759535712476 1106 96-97 0.702238607673813 1106 98-99 0.6021104999659492 1106 100-101 0.6722244391909271 1107 1 0.15917878366840554 1107 2 0.19104792801286408 1107 3 0.13162556269879389 1107 4 0.1609412406642008 1107 5 0.16755945422333696 1107 6 0.16176117876564433 1107 7 0.16316577278486477 1107 8 0.19951094664312308 1107 9 0.27482603400271444 1107 10-11 0.08440922264697548 1107 12-13 0.32334543104352065 1107 14-15 0.15666647188532323 1107 16-17 0.2004688125298415 1107 18-19 0.28594944456819604 1107 20-21 0.27143945676242254 1107 22-23 0.22555623111345113 1107 24-25 0.23546643015583868 1107 26-27 0.26143236304907447 1107 28-29 0.2334775760311345 1107 30-31 0.40385422410122374 1107 32-33 0.015714692359026117 1107 34-35 0.25855184711088697 1107 36-37 0.43607826965953933 1107 38-39 0.4047414405982366 1107 40-41 0.3758600522990321 1107 42-43 0.2991838805936027 1107 44-45 0.42579451907012356 1107 46-47 0.5135860732676889 1107 48-49 0.3695533155982744 1107 50-51 0.4018491618449005 1107 52-53 0.5317709522787837 1107 54-55 0.42964174069224015 1107 56-57 0.520545230486178 1107 58-59 0.4074755835648105 1107 60-61 0.43698188676911087 1107 62-63 0.4285444214699865 1107 64-65 0.38511716778137384 1107 66-67 0.3603401708959453 1107 68-69 0.4308057680980042 1107 70-71 0.556778767909293 1107 72-73 0.3352258422642578 1107 74-75 0.4016641157253673 1107 76-77 0.5451953981021873 1107 78-79 0.7449503960341524 1107 80-81 0.725091441270532 1107 82-83 0.7820504832833883 1107 84-85 0.5943853130827073 1107 86-87 0.7026957467232542 1107 88-89 0.6041242392863424 1107 90-91 0.7231514239382442 1107 92-93 0.3684738573220834 1107 94-95 0.6063240397640861 1107 96-97 0.7041720545760697 1107 98-99 0.7668906843233252 1107 100-101 1.2000065576356285 1108 1 0.1394448078808601 1108 2 0.18218965288870592 1108 3 0.12534164799847503 1108 4 0.10435966684649856 1108 5 0.10782484289448035 1108 6 0.1828205926794837 1108 7 0.10867688597728176 1108 8 0.09327645364202652 1108 9 0.211525283311353 1108 10-11 0.1673693148339126 1108 12-13 0.15702217968107846 1108 14-15 0.2570504308226589 1108 16-17 0.16445557152839285 1108 18-19 0.15969602748835854 1108 20-21 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-2.706423555570243 2316 76-77 -1.7489567959698675 2316 78-79 -1.609196188357199 2316 80-81 -1.3553553869701993 2316 82-83 -0.9246748369835878 2316 84-85 -0.9525167693681489 2316 86-87 -1.019240966919721 2316 88-89 -0.9330874059528682 2316 90-91 -0.9830049144285375 2316 92-93 -0.815369689827854 2316 94-95 -1.1838733294101473 2316 96-97 -0.983168384352826 2316 98-99 -0.8453149505437025 2316 100-101 -1.1619661037571696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 12.0 5 29.0 6 52.0 7 172.0 8 427.0 9 816.0 10 1476.0 11 2434.0 12 3472.0 13 4592.0 14 5573.0 15 7110.0 16 8662.0 17 10255.0 18 8906.0 19 6753.0 20 6629.0 21 7016.0 22 7758.0 23 9034.0 24 11205.0 25 14292.0 26 18422.0 27 24075.0 28 30432.0 29 38365.0 30 47727.0 31 57671.0 32 70636.0 33 85721.0 34 106280.0 35 133139.0 36 174547.0 37 243406.0 38 244070.0 39 48627.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.68409859191125 22.625825401996035 15.339992345909334 22.35008366018338 2 14.771256811243891 22.566827462379706 41.2323966896226 21.429519036753803 3 18.086697184942558 27.49353552906564 31.528421099421934 22.891346186569876 4 12.07595974100333 18.85928204742892 40.739797675374795 28.324960536192954 5 11.648846839313173 39.2868503704033 36.778137245777486 12.286165544506042 6 27.929576672429015 41.120719652008916 18.337874451228533 12.61182922433354 7 24.67554836944216 34.05703792295394 24.020394300930537 17.24701940667336 8 23.48142314012621 38.4443833249294 20.977606643135655 17.096586891808734 9 24.424127005293737 17.27646765484986 22.044514083069064 36.25489125678734 10-11 22.96393839759858 28.350467490939803 29.833089551844893 18.85250455961673 12-13 23.820229557428945 25.529130846067616 30.22841839293131 20.42222120357213 14-15 20.976042738853767 27.496294660282512 27.689861329310588 23.83780127155313 16-17 20.131801746614496 30.670246295702086 28.49236081882799 20.705591138855436 18-19 19.982928350467493 29.38615682303409 30.856555082809304 19.774359743689114 20-21 20.664578896996417 28.631996910712076 30.922917821329698 19.780506370961803 22-23 20.452149308381927 28.30157687253614 30.839616561051024 20.40665725803091 24-25 20.35247828699025 28.59630297158296 30.892829237292553 20.15838950413424 26-27 20.283814489520434 28.818564800071677 30.691769602060315 20.20585110834757 28-29 20.266285134844182 28.748654336336045 30.811739048902286 20.17332147991749 30-31 20.67692282046313 28.507641411078954 30.71994504490801 20.09549072354991 32-33 20.45991104658332 28.79346725552138 30.532417980330212 20.214203717565088 34-35 20.55183561421173 28.699091804849008 30.739396372706977 20.00967620823228 36-37 20.595244048462423 28.586688669783776 30.722477350958055 20.09558993079575 38-39 20.432030576352155 28.83191514409412 30.733803975606428 20.002250303947303 40-41 20.569837850001875 28.68553346418684 30.671742449183135 20.072886236628147 42-43 20.487454519943153 28.740443804779243 30.392139156818153 20.37996251845945 44-45 20.455164270334098 28.880287559908314 30.3769188025283 20.287629367229286 46-47 20.629883083830673 28.800643761322085 30.121715900376756 20.44775725447049 48-49 20.620232361386833 28.978108628878886 29.989601668178604 20.412057341555677 50-51 20.69225989129096 29.166060678599937 29.64889109402824 20.492788336080867 52-53 20.75965174487388 29.18345416157784 29.570118450269554 20.486775643278726 54-55 20.704320843148274 29.133129150148275 29.71865650335485 20.4438935033486 56-57 20.684921230828646 29.271389506102953 29.59683631390423 20.446852949164175 58-59 20.58980134451505 29.18729952537334 29.757580914902377 20.46531821520924 60-61 20.689614409633307 29.319095037979103 29.659754390100506 20.331536162287083 62-63 20.672417435808548 29.186911135266985 29.76606043772257 20.374610991201898 64-65 20.477589621152287 29.046592521763287 29.75957962199814 20.71623823508629 66-67 20.49841707704765 29.14893261489681 29.614027196893055 20.73862311116248 68-69 20.54618272579643 29.169290536836005 29.6431362515295 20.641390485838063 70-71 20.64462010226209 28.926004434373702 29.703516521000623 20.725858942363583 72-73 20.68844742126119 29.132654889018045 29.559665083769055 20.61923260595171 74-75 20.51828278733627 29.404473846608187 29.42582199060821 20.651421375447335 76-77 20.530858079146697 29.252600102737297 29.49016269522522 20.726379122890787 78-79 20.48290639846637 29.305663601255798 29.45308810046398 20.758341899813853 80-81 20.558989257632764 29.32780159645732 29.308007359289018 20.805201786620902 82-83 20.678816847176094 29.25520240808882 29.48745462955632 20.578526115178768 84-85 20.704827151284817 29.274441004185263 29.275864799203234 20.744867045326686 86-87 20.534562103160546 29.391018562119832 29.3466725563163 20.727746778403322 88-89 20.669081337120872 29.253130621401436 29.300879977219218 20.77690806425848 90-91 20.544694488424696 29.192390627512417 29.532768248369738 20.730146635693146 92-93 20.7050575808139 29.256075890804155 29.325959228699126 20.712907299682815 94-95 20.426831174080913 29.13209235066917 29.42269371630111 21.018382758948807 96-97 20.477513233484753 29.165131456261523 29.407593414008133 20.94976189624559 98-99 20.39305076905767 29.275621712248945 29.275482805417923 21.055844713275466 100-101 20.466879749745452 29.184394650975754 29.25652202298352 21.092203576295272 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 138.0 1 100.0 2 85.5 3 120.5 4 98.0 5 101.0 6 170.0 7 354.0 8 683.0 9 930.5 10 869.0 11 764.5 12 823.0 13 911.5 14 1035.0 15 1157.5 16 1346.0 17 1572.5 18 1762.5 19 1952.5 20 2274.5 21 2700.0 22 3307.0 23 4337.0 24 5640.0 25 7384.0 26 10084.5 27 13419.5 28 16938.5 29 21466.5 30 26395.5 31 30868.5 32 36413.0 33 41952.5 34 46537.0 35 50527.0 36 55622.5 37 60891.5 38 62402.5 39 62236.5 40 61936.5 41 60561.0 42 58666.5 43 58470.5 44 59066.5 45 59238.5 46 57615.5 47 54596.0 48 52597.5 49 50106.5 50 46717.5 51 42641.5 52 38009.0 53 33668.5 54 29684.0 55 25076.0 56 20181.5 57 15778.5 58 12289.5 59 9254.0 60 6369.5 61 4290.5 62 2868.5 63 1735.5 64 965.5 65 542.5 66 281.0 67 142.0 68 71.0 69 41.0 70 19.5 71 10.5 72 8.0 73 4.5 74 3.0 75 2.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004236658346147488 2 0.0068758881355508415 3 0.0014585217257229058 4 0.16814671895119787 5 0.002222509296339666 6 4.8617390857430196E-4 7 0.0 8 8.334409861273748E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.3890683102122913E-4 24-25 6.250807395955311E-4 26-27 0.002465596250626817 28-29 2.7781366204245825E-4 30-31 0.0064591676424871555 32-33 0.02059293769889722 34-35 0.01392540980987822 36-37 0.0012501614791910621 38-39 5.903540318402239E-4 40-41 0.0025003229583821242 42-43 0.011945987467825705 44-45 0.00791768936821006 46-47 0.010418012326592185 48-49 0.01111254648169833 50-51 0.1921776007178705 52-53 0.11220199275739783 54-55 0.017849527786227944 56-57 0.007014794966572071 58-59 0.01816206815602571 60-61 0.11949460138601237 62-63 0.2299255320478895 64-65 0.2356207121197599 66-67 0.24635126481614986 68-69 0.2418020661002046 70-71 0.22982135192462358 72-73 0.15699944576174424 74-75 0.2842033762694348 76-77 0.2180837247033297 78-79 0.006528621057997769 80-81 6.945341551061456E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.7781366204245825E-4 90-91 0.006216080688200003 92-93 0.018891329018887165 94-95 0.002847590035935197 96-97 3.472670775530728E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1439814.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.24797244692954 #Duplication Level Percentage of deduplicated Percentage of total 1 79.77539834813996 51.25407595014124 2 12.94534591775085 16.63424455679257 3 3.441953530001593 6.634156066774627 4 1.3267470947096829 3.409632431398061 5 0.6807732987889717 2.1869152071599594 6 0.40534406803116585 1.5625520708635605 7 0.2568244213716117 1.1550313843587352 8 0.17590540689294057 0.904125258825886 9 0.13043523750875832 0.7542179591014273 >10 0.7713944410110269 9.162640816218873 >50 0.06226915730159613 2.722473673262345 >100 0.026851211513192173 2.8286991027684403 >500 4.3306684490984637E-4 0.18294060868036083 >1k 3.2480013368238475E-4 0.6082949136538994 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3700 0.2569776373892739 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3199 0.22218147621845602 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1846 0.1282110050325945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.472670775530728E-5 0.0 14-15 0.0 0.0 0.0 6.945341551061456E-5 0.0 16-17 0.0 0.0 0.0 6.945341551061456E-5 0.0 18-19 0.0 0.0 0.0 6.945341551061456E-5 3.472670775530728E-5 20-21 0.0 0.0 0.0 1.3890683102122913E-4 6.945341551061456E-5 22-23 0.0 0.0 0.0 2.083602465318437E-4 6.945341551061456E-5 24-25 0.0 0.0 0.0 2.4308695428715098E-4 6.945341551061456E-5 26-27 0.0 0.0 0.0 2.7781366204245825E-4 6.945341551061456E-5 28-29 0.0 0.0 0.0 6.250807395955312E-4 6.945341551061456E-5 30-31 0.0 0.0 0.0 0.002326689419605588 6.945341551061456E-5 32-33 0.0 0.0 0.0 0.005695180071870394 6.945341551061456E-5 34-35 0.0 0.0 0.0 0.010904186235166487 6.945341551061456E-5 36-37 0.0 0.0 0.0 0.01969004329725923 6.945341551061456E-5 38-39 0.0 0.0 0.0 0.033997446892445826 6.945341551061456E-5 40-41 0.0 0.0 0.0 0.0514997076011207 6.945341551061456E-5 42-43 0.0 0.0 0.0 0.06969650246490172 6.945341551061456E-5 44-45 0.0 0.0 0.0 0.09178268859727715 1.0418012326592185E-4 46-47 0.0 0.0 0.0 0.11942514797050174 1.3890683102122913E-4 48-49 0.0 0.0 0.0 0.15008883091843808 1.3890683102122913E-4 50-51 0.0 0.0 0.0 0.1806830604508638 1.3890683102122913E-4 52-53 0.0 0.0 0.0 0.21353452598738448 1.3890683102122913E-4 54-55 0.0 0.0 0.0 0.2505184697467867 1.3890683102122913E-4 56-57 0.0 0.0 0.0 0.2960799103217499 1.3890683102122913E-4 58-59 0.0 0.0 0.0 0.3486214191555298 1.3890683102122913E-4 60-61 0.0 0.0 0.0 0.4040105180252449 1.3890683102122913E-4 62-63 0.0 0.0 0.0 0.4610317721594595 1.3890683102122913E-4 64-65 0.0 0.0 0.0 0.5203449890055243 1.3890683102122913E-4 66-67 0.0 0.0 0.0 0.5918125535659466 1.3890683102122913E-4 68-69 0.0 0.0 0.0 0.6743232111925568 1.3890683102122913E-4 70-71 0.0 0.0 0.0 0.764994645141664 1.3890683102122913E-4 72-73 0.0 0.0 0.0 0.8572982343552709 1.3890683102122913E-4 74-75 0.0 0.0 0.0 0.9558179042570776 1.3890683102122913E-4 76-77 0.0 0.0 0.0 1.0654501206405826 1.3890683102122913E-4 78-79 0.0 0.0 0.0 1.2005370138087281 1.3890683102122913E-4 80-81 0.0 0.0 0.0 1.345382111856115 1.3890683102122913E-4 82-83 0.0 0.0 0.0 1.499533967581924 1.3890683102122913E-4 84-85 0.0 0.0 0.0 1.6565681400514234 1.3890683102122913E-4 86-87 0.0 0.0 0.0 1.8245064987560893 1.3890683102122913E-4 88-89 0.0 0.0 0.0 2.0198095031719374 1.3890683102122913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2110 0.0 63.06217 1 GTATCAA 3880 0.0 38.703358 1 ATCAACG 4800 0.0 30.394182 3 TCAACGC 4880 0.0 29.992252 4 CAACGCA 4870 0.0 29.907366 5 TATCAAC 5170 0.0 28.770481 2 AACGCAG 5120 0.0 28.72403 6 CGCAGAG 5735 0.0 25.147442 8 ACGCAGA 5770 0.0 25.077122 7 TGGTATC 1010 0.0 23.055153 2 GTGGTAT 1110 0.0 22.262486 1 GCAGAGT 6615 0.0 21.58615 9 GAGTACT 4545 0.0 21.240713 12-13 GTACATG 3620 0.0 21.135406 1 AGAGTAC 6020 0.0 20.84336 10-11 CAGAGTA 6365 0.0 20.794304 10-11 GTGATCG 195 1.0440883E-5 19.462967 8 ACATGGG 3590 0.0 19.458807 3 GTACTTT 5010 0.0 19.458649 14-15 TACATGG 3830 0.0 19.356157 2 >>END_MODULE