FastQCFastQC Report
Sat 18 Jun 2016
SRR1931031_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931031_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304447
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1424874.31197716289594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.057.4219171
GTATCAA47300.049.5295561
ATCAACG55150.040.309313
CAACGCA56200.038.6491365
TCAACGC56400.038.4306834
TATCAAC62350.035.9284442
AACGCAG63500.034.1241156
ACGCAGA70900.029.9697427
CGCAGAG71050.029.0483138
GTACATG53200.025.5758551
GCAGAGT80900.023.9207889
TACATGG55050.023.2264822
TCAACGA3350.022.517824
ACATGGG54150.022.384493
AGAGTAC72700.021.98328410-11
GAGTACT53300.020.21503812-13
CAGAGTA78600.019.80803710-11
AGTACTT61750.019.46956312-13
GAGTACA39350.018.3288211
GTACTTT62600.017.65112314-15
Produced by FastQC (version 0.11.5)