##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931031_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3304447 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.96452719622981 28.0 2.0 31.0 2.0 31.0 2 20.375398969933546 28.0 2.0 31.0 2.0 33.0 3 21.10395687992575 30.0 2.0 31.0 2.0 33.0 4 22.800677692818194 32.0 2.0 35.0 2.0 37.0 5 22.009199118642243 32.0 2.0 35.0 2.0 37.0 6 22.56090474442471 32.0 2.0 35.0 2.0 37.0 7 22.276465623446224 30.0 2.0 35.0 2.0 37.0 8 21.98163747217008 29.0 2.0 35.0 2.0 37.0 9 20.762313331095946 26.0 2.0 33.0 2.0 37.0 10-11 20.785005327669047 26.0 2.0 33.5 2.0 37.0 12-13 20.418781417889285 25.5 2.0 33.0 2.0 37.0 14-15 21.961135100668887 28.5 2.0 35.0 2.0 37.5 16-17 23.26460872273031 31.5 2.0 36.5 2.0 38.5 18-19 23.45456562020816 32.0 2.0 37.5 2.0 39.5 20-21 23.742421651792267 32.0 2.0 38.0 2.0 40.0 22-23 23.72318409101432 32.0 2.0 38.0 2.0 40.0 24-25 23.78650769705188 32.0 2.0 38.0 2.0 40.0 26-27 23.631504303140588 32.0 2.0 38.0 2.0 40.0 28-29 23.559215354339166 32.0 2.0 38.0 2.0 40.0 30-31 23.686036574349657 32.0 2.0 38.0 2.0 40.0 32-33 23.62926065995309 32.0 2.0 38.0 2.0 40.0 34-35 23.36431012511322 31.0 2.0 38.0 2.0 40.0 36-37 22.916140128741663 30.0 2.0 38.0 2.0 40.0 38-39 22.90907843279072 30.0 2.0 38.0 2.0 40.0 40-41 22.883367171572125 30.0 2.0 38.0 2.0 40.0 42-43 22.819746844176954 30.0 2.0 38.0 2.0 40.0 44-45 22.48892779941697 30.0 2.0 38.0 2.0 40.0 46-47 22.191972665925647 30.0 2.0 38.0 2.0 40.0 48-49 22.063582499583138 29.5 2.0 38.0 2.0 40.0 50-51 19.295004882813977 19.5 2.0 35.5 2.0 38.5 52-53 21.561774784101544 28.5 2.0 37.0 2.0 39.0 54-55 22.51188095920437 31.0 2.0 38.0 2.0 40.0 56-57 22.56610970004966 31.0 2.0 38.0 2.0 40.0 58-59 22.331098667946556 30.5 2.0 38.0 2.0 40.0 60-61 22.091723970758196 30.0 2.0 38.0 2.0 40.0 62-63 21.691304929387577 29.0 2.0 37.5 2.0 40.0 64-65 21.40223296061338 28.0 2.0 37.0 2.0 40.0 66-67 21.111350250132624 28.0 2.0 36.5 2.0 39.0 68-69 20.70421223278812 28.0 2.0 36.0 2.0 39.0 70-71 20.410072093757293 27.0 2.0 35.5 2.0 39.0 72-73 20.044260355817478 26.0 2.0 35.0 2.0 38.5 74-75 19.619143082034604 26.0 2.0 35.0 2.0 38.0 76-77 19.253024484883554 26.0 2.0 34.0 2.0 37.0 78-79 18.92631838247065 25.0 2.0 34.0 2.0 36.5 80-81 18.53023864507435 24.0 2.0 34.0 2.0 36.0 82-83 18.104844925641114 22.0 2.0 34.0 2.0 36.0 84-85 17.760781153397225 20.5 2.0 33.5 2.0 35.0 86-87 17.300969723527114 19.0 2.0 33.0 2.0 35.0 88-89 16.920705340409455 17.5 2.0 32.5 2.0 35.0 90-91 16.473928012765825 9.5 2.0 32.0 2.0 35.0 92-93 15.949058647331913 4.5 2.0 32.0 2.0 34.5 94-95 15.409080551148195 2.0 2.0 32.0 2.0 34.0 96-97 14.77329141608263 2.0 2.0 31.0 2.0 34.0 98-99 14.061981475266514 2.0 2.0 31.0 2.0 34.0 100-101 12.859117577010617 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 9.329149179777296 1101 2 10.453607084744757 1101 3 9.773639107033738 1101 4 12.010845047340169 1101 5 12.751133416745258 1101 6 12.074382898883147 1101 7 12.244100689666201 1101 8 12.215296279318643 1101 9 13.176274983226747 1101 10-11 12.846825289475603 1101 12-13 12.327859380983863 1101 14-15 11.68745718725419 1101 16-17 11.240901784503901 1101 18-19 11.918184618559735 1101 20-21 12.043743519335258 1101 22-23 12.38357456553852 1101 24-25 12.325972510371066 1101 26-27 12.545444124244494 1101 28-29 12.631694995984173 1101 30-31 12.219183710995306 1101 32-33 12.353112944995129 1101 34-35 12.478852342228617 1101 36-37 12.758670193116195 1101 38-39 12.430088715201734 1101 40-41 11.998724568288548 1101 42-43 11.928815225029371 1101 44-45 11.946224359299617 1101 46-47 11.949833145251898 1101 48-49 12.0953257217758 1101 50-51 13.9434332811047 1101 52-53 12.646556429757272 1101 54-55 12.389207403592266 1101 56-57 12.348557149369665 1101 58-59 12.224785363847435 1101 60-61 12.070485512169714 1101 62-63 12.252734205921879 1101 64-65 11.930645217793593 1101 66-67 11.772726768950204 1101 68-69 11.952878603497556 1101 70-71 11.677626101474555 1101 72-73 11.645499091860394 1101 74-75 11.362415277650875 1101 76-77 11.47754791289351 1101 78-79 11.07037567295556 1101 80-81 10.895820496785927 1101 82-83 10.740199599419675 1101 84-85 10.582757395367164 1101 86-87 10.539691102414213 1101 88-89 10.409058683853775 1101 90-91 10.100321404723363 1101 92-93 10.011974614064608 1101 94-95 9.611149792664284 1101 96-97 9.713104863568718 1101 98-99 9.684001793702617 1101 100-101 9.129182516821283 1102 1 9.415211785069392 1102 2 9.970037359101067 1102 3 9.471689290801017 1102 4 11.648225968496774 1102 5 12.258075885649415 1102 6 11.395476961501888 1102 7 11.417057308482757 1102 8 11.39619574701561 1102 9 12.042902811611981 1102 10-11 11.070519667508272 1102 12-13 10.651296770647836 1102 14-15 10.16368151784026 1102 16-17 10.364109507101059 1102 18-19 11.363121434896698 1102 20-21 11.584628208309553 1102 22-23 11.97146786618718 1102 24-25 11.909049864122238 1102 26-27 12.141560380166919 1102 28-29 12.093209201379832 1102 30-31 12.13400724060487 1102 32-33 11.897693071227952 1102 34-35 11.906972014574752 1102 36-37 12.244106005710556 1102 38-39 11.944032667022093 1102 40-41 11.656927515962789 1102 42-43 11.308550553069939 1102 44-45 11.650684708945032 1102 46-47 11.476282603365402 1102 48-49 11.049593225062765 1102 50-51 13.31729057816207 1102 52-53 11.956075339460792 1102 54-55 11.645466335556343 1102 56-57 11.529232750522372 1102 58-59 11.598069417958744 1102 60-61 11.53269082040443 1102 62-63 11.655491219510228 1102 64-65 11.454979250552253 1102 66-67 11.444848616912626 1102 68-69 11.119354693575467 1102 70-71 10.791578207652702 1102 72-73 10.878005976828465 1102 74-75 10.609285685570526 1102 76-77 10.334145040541998 1102 78-79 10.187512938538791 1102 80-81 9.960139212802432 1102 82-83 9.9939138673601 1102 84-85 9.92922033759396 1102 86-87 9.821232662737966 1102 88-89 9.693311694240915 1102 90-91 9.512510152045873 1102 92-93 9.513484431238863 1102 94-95 9.25175193734445 1102 96-97 9.228265832272795 1102 98-99 8.969170193800807 1102 100-101 8.030868871638402 1103 1 9.089523777333415 1103 2 10.039787570891775 1103 3 9.31606872321856 1103 4 11.496236633760937 1103 5 12.168767280372375 1103 6 11.180794580990966 1103 7 11.11667675095308 1103 8 10.76633841656053 1103 9 11.028310556577686 1103 10-11 10.318861375381893 1103 12-13 9.578013398839882 1103 14-15 9.668882290439456 1103 16-17 10.285171281749083 1103 18-19 11.2910918307997 1103 20-21 11.797429562771477 1103 22-23 12.242818325302832 1103 24-25 12.2826076900594 1103 26-27 12.324940218690028 1103 28-29 12.066488994798586 1103 30-31 12.067240531607805 1103 32-33 12.268128170066387 1103 34-35 12.217378011955038 1103 36-37 12.47576409811289 1103 38-39 12.139942817830388 1103 40-41 11.85906115132327 1103 42-43 11.567291892119815 1103 44-45 11.73736835770367 1103 46-47 11.680561483046642 1103 48-49 11.681540085151077 1103 50-51 13.812398004734352 1103 52-53 12.471206849424522 1103 54-55 11.86971229405701 1103 56-57 11.733086051544792 1103 58-59 11.698893404484028 1103 60-61 11.754725792085228 1103 62-63 11.785419784875753 1103 64-65 11.516434964241181 1103 66-67 11.433917719768846 1103 68-69 11.316315434485109 1103 70-71 11.23902311294189 1103 72-73 11.026602457690739 1103 74-75 10.918634768254616 1103 76-77 10.680096301216828 1103 78-79 10.364892109650704 1103 80-81 10.215239083148237 1103 82-83 10.147030186645623 1103 84-85 9.990842654426203 1103 86-87 9.899776474704435 1103 88-89 9.717261437012983 1103 90-91 9.37849402599337 1103 92-93 9.279137387404427 1103 94-95 9.267955799008918 1103 96-97 9.009488567859796 1103 98-99 8.857442272878213 1103 100-101 8.214918160204702 1104 1 8.831148635224025 1104 2 9.929478861120074 1104 3 9.321539256680353 1104 4 11.286718593432447 1104 5 11.474144055991385 1104 6 10.900923522735976 1104 7 10.538436767359695 1104 8 10.265904745352334 1104 9 9.859998393026466 1104 10-11 9.076044956323955 1104 12-13 8.639958667950285 1104 14-15 9.015302652646724 1104 16-17 9.969688154825786 1104 18-19 11.261615729566941 1104 20-21 11.530446491177116 1104 22-23 11.902471489591857 1104 24-25 11.969464611669665 1104 26-27 12.189039201963485 1104 28-29 12.144459526050444 1104 30-31 11.944343744551734 1104 32-33 12.049898895455723 1104 34-35 11.921880526026552 1104 36-37 12.126520232171735 1104 38-39 11.748831016324118 1104 40-41 11.554085982646338 1104 42-43 11.12093094925682 1104 44-45 11.22303350117036 1104 46-47 11.47743435723201 1104 48-49 11.320486283000136 1104 50-51 13.322206857509467 1104 52-53 12.08157960736688 1104 54-55 11.740190659959154 1104 56-57 11.584994988386146 1104 58-59 11.594277789540019 1104 60-61 11.3994049441003 1104 62-63 11.490722860899648 1104 64-65 11.296798317469086 1104 66-67 11.203099122269489 1104 68-69 11.052865338738318 1104 70-71 10.814690923609074 1104 72-73 10.64743632308463 1104 74-75 10.703779594669207 1104 76-77 10.248991078108602 1104 78-79 10.097121494678174 1104 80-81 9.86555383128825 1104 82-83 9.70312995521234 1104 84-85 9.446164876593958 1104 86-87 9.172748733328216 1104 88-89 8.851834358266121 1104 90-91 8.575191302113872 1104 92-93 8.405213840669072 1104 94-95 8.30637847337926 1104 96-97 8.001864384524195 1104 98-99 7.694404279294439 1104 100-101 7.068739822935397 1105 1 8.592142137953502 1105 2 9.389529254488913 1105 3 8.895657519104507 1105 4 10.961173136935273 1105 5 10.636700729014144 1105 6 10.525609949900772 1105 7 10.016508781864868 1105 8 9.47983494019238 1105 9 8.597113167729852 1105 10-11 8.202955093354099 1105 12-13 7.570145349814336 1105 14-15 8.513787221385272 1105 16-17 9.865249205552896 1105 18-19 10.765869929176986 1105 20-21 11.584559288789777 1105 22-23 11.987423038182808 1105 24-25 12.125620663265295 1105 26-27 12.280721017217726 1105 28-29 11.899278767651705 1105 30-31 11.760380780359913 1105 32-33 11.527288042730437 1105 34-35 11.686554657365487 1105 36-37 11.886722117922048 1105 38-39 11.53435078820727 1105 40-41 11.31602731789496 1105 42-43 11.275231502178631 1105 44-45 11.264498929406653 1105 46-47 11.27772344576 1105 48-49 11.117466623340789 1105 50-51 12.945185804323192 1105 52-53 11.481873067744065 1105 54-55 11.14412614395603 1105 56-57 11.187887708281377 1105 58-59 11.183794992961769 1105 60-61 11.066690982092457 1105 62-63 11.075777811530923 1105 64-65 10.953808831376055 1105 66-67 10.547274745697134 1105 68-69 10.609922420646086 1105 70-71 10.506107695505886 1105 72-73 10.436127364111702 1105 74-75 10.307348663055468 1105 76-77 9.944746519987405 1105 78-79 9.580774994402002 1105 80-81 9.380701078682218 1105 82-83 9.428898102777865 1105 84-85 9.165985162364564 1105 86-87 8.934723911373723 1105 88-89 8.813955212572292 1105 90-91 8.38622998712409 1105 92-93 8.372644461959586 1105 94-95 8.138298281174032 1105 96-97 7.891287983531944 1105 98-99 7.53768933362052 1105 100-101 6.825658879318587 1106 1 8.447126795554148 1106 2 9.42264546192597 1106 3 8.892541350756364 1106 4 10.591074202889999 1106 5 10.788240636842485 1106 6 10.10799695361813 1106 7 9.469038264679426 1106 8 9.023870167123079 1106 9 7.792674699546275 1106 10-11 7.466033032742828 1106 12-13 7.188015785694834 1106 14-15 8.348009879666062 1106 16-17 9.645455125960044 1106 18-19 11.160757841711629 1106 20-21 11.529267978646587 1106 22-23 11.898279515411563 1106 24-25 11.698687970468981 1106 26-27 11.902699502678605 1106 28-29 11.930928710777334 1106 30-31 11.33299180265232 1106 32-33 11.541735501364595 1106 34-35 11.477622214738243 1106 36-37 11.713077271991011 1106 38-39 11.465665348437426 1106 40-41 11.428656321088521 1106 42-43 11.283264039791948 1106 44-45 10.838782331862216 1106 46-47 10.762096440010023 1106 48-49 10.957312847546987 1106 50-51 13.112706042609222 1106 52-53 11.880777679063439 1106 54-55 11.301916721182053 1106 56-57 11.227085799178742 1106 58-59 11.080936537833896 1106 60-61 10.973634189807047 1106 62-63 11.138862786924452 1106 64-65 11.075639951625288 1106 66-67 10.82869789493471 1106 68-69 10.572600363161925 1106 70-71 10.32377682347127 1106 72-73 10.092896508885321 1106 74-75 9.898609006959745 1106 76-77 9.803233270799478 1106 78-79 9.504674817329214 1106 80-81 9.110706746574973 1106 82-83 9.104143288000692 1106 84-85 8.678794404547759 1106 86-87 8.623342860898209 1106 88-89 8.369526164817366 1106 90-91 8.126138636328434 1106 92-93 7.922238797193943 1106 94-95 7.756719675319294 1106 96-97 7.346940084365711 1106 98-99 6.9380362086571665 1106 100-101 6.349568083585533 1107 1 7.840079698578755 1107 2 8.626101802789066 1107 3 8.600355890785853 1107 4 10.218898331082457 1107 5 10.495913522918539 1107 6 9.942508786481444 1107 7 9.44298602775601 1107 8 8.302187190230924 1107 9 4.728265208544187 1107 10-11 6.4490563992488745 1107 12-13 6.675962017099046 1107 14-15 7.634336981421498 1107 16-17 9.130168199481101 1107 18-19 10.821481763616617 1107 20-21 11.229023102954955 1107 22-23 11.12700400424438 1107 24-25 11.30451903533698 1107 26-27 11.361101719406971 1107 28-29 11.506865331241624 1107 30-31 11.13161697927124 1107 32-33 11.325785024397948 1107 34-35 11.32922074372122 1107 36-37 11.792163547829006 1107 38-39 11.567139696201309 1107 40-41 11.179120869612571 1107 42-43 10.825787214955955 1107 44-45 10.517898139741387 1107 46-47 10.70224596761437 1107 48-49 10.81420662907884 1107 50-51 12.696753326948485 1107 52-53 11.328439780406232 1107 54-55 11.205303693729732 1107 56-57 10.792363115653796 1107 58-59 10.744291744326507 1107 60-61 10.549558446751714 1107 62-63 10.74511861864455 1107 64-65 10.836429911105707 1107 66-67 10.727519998630083 1107 68-69 10.4571355034048 1107 70-71 10.282594914678008 1107 72-73 10.137109781080724 1107 74-75 10.204616881858712 1107 76-77 9.911703888602602 1107 78-79 9.704712898529692 1107 80-81 9.287825670529106 1107 82-83 9.05227871323634 1107 84-85 8.810105708299233 1107 86-87 8.615534108711973 1107 88-89 8.466403155363526 1107 90-91 8.163664648471674 1107 92-93 7.7409660036609775 1107 94-95 7.734929966541413 1107 96-97 7.325615431133185 1107 98-99 7.008247427494275 1107 100-101 6.642979287071595 1108 1 6.866262975564645 1108 2 8.142174005859427 1108 3 8.203299461649493 1108 4 9.283750282781117 1108 5 10.221866256007928 1108 6 9.176517103688816 1108 7 8.246542681483255 1108 8 5.768342000998608 1108 9 5.344589680137112 1108 10-11 4.820383565159304 1108 12-13 3.807210417710426 1108 14-15 6.677656426971929 1108 16-17 8.47242255851572 1108 18-19 8.645908090461688 1108 20-21 9.118249891815552 1108 22-23 10.06568311605508 1108 24-25 9.850663957920773 1108 26-27 8.351068730446325 1108 28-29 10.191570769278893 1108 30-31 10.495106387163087 1108 32-33 10.889961657204605 1108 34-35 10.354734732138358 1108 36-37 10.2011224821008 1108 38-39 9.916670490665474 1108 40-41 10.18024985436126 1108 42-43 9.589858896944534 1108 44-45 9.000233969989338 1108 46-47 9.6952700819493 1108 48-49 10.335236028450915 1108 50-51 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-13.982721061826958 2313 92-93 -13.477845048529923 2313 94-95 -12.957785634909884 2313 96-97 -12.334306422062463 2313 98-99 -11.648604372673184 2313 100-101 -10.491963498303791 2314 1 -18.362822897011533 2314 2 -17.780540675581015 2314 3 -18.487559264112278 2314 4 -20.1101555343934 2314 5 -19.293369201055786 2314 6 -19.88082361653279 2314 7 -19.59999471463863 2314 8 -19.334151073793635 2314 9 -18.163166552549868 2314 10-11 -18.199422529023522 2314 12-13 -17.849714790045805 2314 14-15 -19.369989002390952 2314 16-17 -20.6091563888527 2314 18-19 -20.764479721172663 2314 20-21 -21.02620994197946 2314 22-23 -20.997751786992016 2314 24-25 -21.062770945126317 2314 26-27 -20.90627198977528 2314 28-29 -20.852889064516123 2314 30-31 -20.9722066322275 2314 32-33 -20.908376292933898 2314 34-35 -20.666871916061087 2314 36-37 -20.213697462497535 2314 38-39 -20.217817043960565 2314 40-41 -20.188448206580563 2314 42-43 -20.144628637681503 2314 44-45 -19.80682974192202 2314 46-47 -19.52675207871552 2314 48-49 -19.392603306729143 2314 50-51 -16.759009999872614 2314 52-53 -18.924560498689498 2314 54-55 -19.830978751148862 2314 56-57 -19.895189214795547 2314 58-59 -19.65872248955571 2314 60-61 -19.41904328364181 2314 62-63 -19.01820820245509 2314 64-65 -18.74227508470786 2314 66-67 -18.475382596960205 2314 68-69 -18.069518138794475 2314 70-71 -17.787179017780826 2314 72-73 -17.423872635888298 2314 74-75 -17.016147840441036 2314 76-77 -16.666722011481127 2314 78-79 -16.348036194409186 2314 80-81 -15.96587234789121 2314 82-83 -15.562010988131227 2314 84-85 -15.223525327145925 2314 86-87 -14.772408955759143 2314 88-89 -14.408422409805329 2314 90-91 -13.982721061826958 2314 92-93 -13.477845048529923 2314 94-95 -12.957785634909884 2314 96-97 -12.334306422062463 2314 98-99 -11.648604372673184 2314 100-101 -10.491963498303791 2315 1 -18.362822897011533 2315 2 -17.780540675581015 2315 3 -18.487559264112278 2315 4 -20.1101555343934 2315 5 -19.293369201055786 2315 6 -19.88082361653279 2315 7 -19.59999471463863 2315 8 -19.334151073793635 2315 9 -18.163166552549868 2315 10-11 -18.199422529023522 2315 12-13 -17.849714790045805 2315 14-15 -19.369989002390952 2315 16-17 -20.6091563888527 2315 18-19 -20.764479721172663 2315 20-21 -21.02620994197946 2315 22-23 -20.997751786992016 2315 24-25 -21.062770945126317 2315 26-27 -20.90627198977528 2315 28-29 -20.852889064516123 2315 30-31 -20.9722066322275 2315 32-33 -20.908376292933898 2315 34-35 -20.666871916061087 2315 36-37 -20.213697462497535 2315 38-39 -20.217817043960565 2315 40-41 -20.188448206580563 2315 42-43 -20.144628637681503 2315 44-45 -19.80682974192202 2315 46-47 -19.52675207871552 2315 48-49 -19.392603306729143 2315 50-51 -16.759009999872614 2315 52-53 -18.924560498689498 2315 54-55 -19.830978751148862 2315 56-57 -19.895189214795547 2315 58-59 -19.65872248955571 2315 60-61 -19.41904328364181 2315 62-63 -19.01820820245509 2315 64-65 -18.74227508470786 2315 66-67 -18.475382596960205 2315 68-69 -18.069518138794475 2315 70-71 -17.787179017780826 2315 72-73 -17.423872635888298 2315 74-75 -17.016147840441036 2315 76-77 -16.666722011481127 2315 78-79 -16.348036194409186 2315 80-81 -15.96587234789121 2315 82-83 -15.562010988131227 2315 84-85 -15.223525327145925 2315 86-87 -14.772408955759143 2315 88-89 -14.408422409805329 2315 90-91 -13.982721061826958 2315 92-93 -13.477845048529923 2315 94-95 -12.957785634909884 2315 96-97 -12.334306422062463 2315 98-99 -11.648604372673184 2315 100-101 -10.491963498303791 2316 1 -18.362822897011533 2316 2 -17.780540675581015 2316 3 -18.487559264112278 2316 4 -20.1101555343934 2316 5 -19.293369201055786 2316 6 -19.88082361653279 2316 7 -19.59999471463863 2316 8 -19.334151073793635 2316 9 -18.163166552549868 2316 10-11 -18.199422529023522 2316 12-13 -17.849714790045805 2316 14-15 -19.369989002390952 2316 16-17 -20.6091563888527 2316 18-19 -20.764479721172663 2316 20-21 -21.02620994197946 2316 22-23 -20.997751786992016 2316 24-25 -21.062770945126317 2316 26-27 -20.90627198977528 2316 28-29 -20.852889064516123 2316 30-31 -20.9722066322275 2316 32-33 -20.908376292933898 2316 34-35 -20.666871916061087 2316 36-37 -20.213697462497535 2316 38-39 -20.217817043960565 2316 40-41 -20.188448206580563 2316 42-43 -20.144628637681503 2316 44-45 -19.80682974192202 2316 46-47 -19.52675207871552 2316 48-49 -19.392603306729143 2316 50-51 -16.759009999872614 2316 52-53 -18.924560498689498 2316 54-55 -19.830978751148862 2316 56-57 -19.895189214795547 2316 58-59 -19.65872248955571 2316 60-61 -19.41904328364181 2316 62-63 -19.01820820245509 2316 64-65 -18.74227508470786 2316 66-67 -18.475382596960205 2316 68-69 -18.069518138794475 2316 70-71 -17.787179017780826 2316 72-73 -17.423872635888298 2316 74-75 -17.016147840441036 2316 76-77 -16.666722011481127 2316 78-79 -16.348036194409186 2316 80-81 -15.96587234789121 2316 82-83 -15.562010988131227 2316 84-85 -15.223525327145925 2316 86-87 -14.772408955759143 2316 88-89 -14.408422409805329 2316 90-91 -13.982721061826958 2316 92-93 -13.477845048529923 2316 94-95 -12.957785634909884 2316 96-97 -12.334306422062463 2316 98-99 -11.648604372673184 2316 100-101 -10.491963498303791 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 902974.0 3 15168.0 4 39032.0 5 22464.0 6 21567.0 7 21323.0 8 17658.0 9 19060.0 10 21775.0 11 24615.0 12 27335.0 13 29387.0 14 30853.0 15 33204.0 16 34862.0 17 34354.0 18 33706.0 19 35939.0 20 39014.0 21 42085.0 22 46625.0 23 51862.0 24 57310.0 25 63562.0 26 70892.0 27 79715.0 28 89874.0 29 101773.0 30 116438.0 31 133414.0 32 153303.0 33 177047.0 34 200812.0 35 215107.0 36 188491.0 37 97139.0 38 14589.0 39 119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.38989953674463 21.491517267387113 14.699161636563165 23.419421559305093 2 13.880239531083157 21.07568326638829 42.47149375513079 22.57258344739777 3 17.452284383091826 27.25216660951833 31.206338444509225 24.08921056288062 4 10.995185575600258 17.645328798061197 42.37480571129449 28.984679915044048 5 11.510311119599084 38.65909468688219 37.554773954653 12.275820238865725 6 27.40494178362792 40.82211906234792 19.210143168648532 12.562795985375628 7 23.73928203527638 35.48981266270538 24.28831295550611 16.482592346512128 8 22.591610540316662 39.55209916486391 21.335025279825526 16.5212650149939 9 24.00753050428143 20.12356804612041 21.615491986797363 34.253409462800796 10-11 21.909693295703462 30.97204011607681 29.164351557069796 17.953915031149933 12-13 22.737947328721074 28.34910640256797 29.258844492775367 19.654101775935597 14-15 20.491797142288178 28.617619711413926 27.43882855675521 23.451754589542688 16-17 20.049556992877935 30.9021023565269 28.374084433777874 20.674256216817295 18-19 19.950278034021416 29.68581274493693 30.457501099309102 19.906408121732554 20-21 20.814015566015673 28.73415620034493 30.43595967887034 20.015868554769057 22-23 20.47348829999602 28.535588079423036 30.136344688757482 20.85457893182347 24-25 20.408006893923755 28.66083066029451 30.46136495992753 20.469797485854208 26-27 20.40872186251919 28.816516410925804 30.19875474363681 20.576006982918198 28-29 20.379972059576193 28.90091611113078 30.258243712599906 20.460868116693113 30-31 20.708908433673862 28.674787879961894 30.14386842076521 20.47243526559904 32-33 20.578024238833567 28.770695458555373 30.003816818742614 20.647463483868446 34-35 20.68190471730889 28.781971166103933 30.050272114660974 20.4858520019262 36-37 20.70710216094624 28.62606234631397 30.108067934072043 20.55876755866775 38-39 20.594723073510405 28.802015497919935 30.18543968810839 20.41782174046127 40-41 20.790159160244738 28.70514544659247 30.05218560898215 20.452509784180638 42-43 20.675730267964635 28.730298298510647 29.824929343163113 20.7690420903616 44-45 20.658959226682814 29.044221021664224 29.662419190718804 20.634400560934164 46-47 20.774568729579084 28.81411513716794 29.50600814836173 20.90530798489125 48-49 20.730089428242447 28.86111822894275 29.542131206363347 20.866661136451455 50-51 21.082918811874553 29.005688814873515 29.04843241359552 20.86295995965641 52-53 20.578637012305123 29.24980671069688 29.1478922811215 21.023663995876497 54-55 20.575132153743148 29.11966956491882 29.26288489241195 21.042313388926086 56-57 20.6741095976363 29.18112590952655 29.253052102187315 20.891712390649836 58-59 20.579166794913675 29.169942343455084 29.38498030622407 20.865910555407176 60-61 20.623966311653916 29.19675741378701 29.32709170951105 20.852184565048027 62-63 20.559746109980008 29.04565328759647 29.253092132824676 21.141508469598843 64-65 20.39867253564916 29.06066946861004 29.29627717771285 21.244380818027956 66-67 20.48975240924046 29.016004003919853 29.11851493803031 21.37572864880937 68-69 20.480454967099845 29.063793751610724 29.06531088656638 21.39044039472305 70-71 20.43659511867906 28.76412748152179 29.286997739216332 21.512279660582816 72-73 20.50306820670288 28.855034589074464 29.250037864207854 21.3918593400148 74-75 20.42639285549503 29.059865621170054 28.914574045186093 21.59916747814882 76-77 20.41055559787749 28.927594376687775 29.10134883736103 21.560501188073708 78-79 20.261207509075216 28.991846685569765 29.06899289833424 21.67795290702078 80-81 20.33098072150352 28.98644268017784 28.982116612331254 21.700459985987386 82-83 20.431468556055886 28.74255498399641 29.227761884587274 21.598214575360434 84-85 20.59616630469483 28.846672772095474 28.905338033540435 21.651822889669262 86-87 20.30977991379639 28.877007285349197 29.081740855170306 21.731471945684095 88-89 20.483827558105617 28.689939736754265 29.015294354681075 21.81093835045904 90-91 20.404618359620983 28.70689019127632 29.157222387769178 21.731269061333524 92-93 20.436070687259264 28.555725712212325 29.006592151771947 22.001611448756464 94-95 20.14435947759709 28.609665267803848 29.137837731912764 22.1081375226863 96-97 20.190680569428014 28.54676287880182 29.051934216002902 22.210622335767262 98-99 20.12443963504055 28.44150770629264 28.8211155795345 22.612937079132305 100-101 20.255141502698798 28.441950156178713 28.831313803741885 22.471594537380604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 193533.0 1 175649.5 2 117218.5 3 60092.5 4 36749.0 5 28355.0 6 27066.5 7 28053.5 8 28849.5 9 27896.5 10 24641.5 11 20078.5 12 15355.0 13 11356.0 14 8833.5 15 7762.5 16 7828.5 17 8669.0 18 9994.5 19 11715.5 20 13650.5 21 15625.0 22 17617.0 23 19991.5 24 22600.5 25 25887.0 26 30203.0 27 35105.0 28 40900.5 29 48289.5 30 55734.0 31 62873.0 32 70577.0 33 76838.5 34 82272.0 35 88013.0 36 94123.5 37 99526.0 38 101715.0 39 101137.5 40 100419.0 41 99821.0 42 99162.5 43 98641.5 44 98153.0 45 97761.0 46 95876.5 47 93128.0 48 89787.5 49 84582.5 50 78256.5 51 71727.0 52 64791.5 53 57568.5 54 49934.0 55 41650.0 56 33576.5 57 26247.0 58 20091.5 59 15126.5 60 10968.5 61 7697.5 62 5309.5 63 3523.0 64 2123.0 65 1220.0 66 699.0 67 402.0 68 241.0 69 131.5 70 86.5 71 47.0 72 28.0 73 19.5 74 12.5 75 8.0 76 4.5 77 3.0 78 5.0 79 3.5 80 0.5 81 0.0 82 0.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.51830548348937 2 28.4109565080027 3 22.246082324818644 4 28.48706606581979 5 31.546065045074105 6 24.32146740437961 7 23.207665306782044 8 19.29811553945335 9 11.413498234349046 10-11 14.92549282830077 12-13 15.066666222820338 14-15 19.15415801796791 16-17 22.275769591704755 18-19 26.154996584905128 20-21 28.180206854581115 22-23 27.76506931416966 24-25 26.340852191002007 26-27 27.747653389508137 28-29 24.78959414389155 30-31 26.57532410112797 32-33 25.629189997600204 34-35 23.73956973738722 36-37 26.62657322087478 38-39 26.73990534573561 40-41 23.862494995380466 42-43 24.67827445863105 44-45 23.477695360222146 46-47 26.635500584515352 48-49 22.112852770826706 50-51 27.74912110861515 52-53 21.32632177184261 54-55 22.44015413168981 56-57 24.51918581233108 58-59 23.80184944712383 60-61 23.342650071252464 62-63 23.17687346778447 64-65 21.765139522588804 66-67 22.241694298622434 68-69 23.20415488582507 70-71 21.7269183013073 72-73 21.575455741913853 74-75 18.997444958263817 76-77 21.146004157427853 78-79 20.290460098164687 80-81 21.302596168133427 82-83 22.106376649406087 84-85 19.141114988377783 86-87 20.661460147492154 88-89 19.492489968820802 90-91 20.633634008958232 92-93 20.600148829743674 94-95 19.696578580319187 96-97 18.60418702433418 98-99 18.004237925438055 100-101 19.67397267984628 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3304447.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.54636956739826 #Duplication Level Percentage of deduplicated Percentage of total 1 84.32401924890544 60.33077444590593 2 10.360133420232692 14.824598689030438 3 2.728151412975757 5.855679876857498 4 1.0358011901850674 2.964312590053272 5 0.5089207234713868 1.8205715080995766 6 0.2855531386217134 1.2258174232175767 7 0.1687620256834528 0.845201718094375 8 0.10864296422520901 0.6218407735483544 9 0.08007592968155516 0.5156227852604586 >10 0.37427941062544406 4.560980898015499 >50 0.017792177553865963 0.863109896628359 >100 0.007440560000001265 0.9069855656543764 >500 2.5667870339946014E-4 0.12922436791490485 >1k 1.2833935123677967E-4 0.1741253972149423 >5k 0.0 0.0 >10k+ 4.2779783745593223E-5 4.361154064504391 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 142487 4.31197716289594 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 3.0262249629060474E-5 0.0 0.0 0.0 7 0.0 3.0262249629060474E-5 0.0 0.0 0.0 8 0.0 3.0262249629060474E-5 0.0 0.0 0.0 9 0.0 3.0262249629060474E-5 0.0 0.0 0.0 10-11 3.0262249629060474E-5 3.0262249629060474E-5 0.0 4.539337444359071E-5 0.0 12-13 1.210489985162419E-4 3.0262249629060474E-5 0.0 6.052449925812095E-5 0.0 14-15 1.210489985162419E-4 3.0262249629060474E-5 0.0 6.052449925812095E-5 0.0 16-17 1.210489985162419E-4 3.0262249629060474E-5 0.0 7.565562407265119E-5 0.0 18-19 1.210489985162419E-4 3.0262249629060474E-5 0.0 1.0591787370171167E-4 0.0 20-21 1.210489985162419E-4 3.0262249629060474E-5 0.0 1.5131124814530238E-4 0.0 22-23 1.210489985162419E-4 3.0262249629060474E-5 0.0 1.8157349777436286E-4 0.0 24-25 1.3618012333077215E-4 3.0262249629060474E-5 0.0 2.723602466615443E-4 0.0 26-27 1.5131124814530238E-4 3.0262249629060474E-5 0.0 3.328847459196652E-4 0.0 28-29 1.5131124814530238E-4 3.0262249629060474E-5 0.0 7.565562407265119E-4 0.0 30-31 1.664423729598326E-4 3.0262249629060474E-5 0.0 0.002799258090688094 0.0 32-33 2.1183574740342334E-4 3.0262249629060474E-5 0.0 0.008125414025402738 0.0 34-35 2.1183574740342334E-4 3.0262249629060474E-5 0.0 0.016462663798208897 0.0 36-37 2.1183574740342334E-4 3.0262249629060474E-5 0.0 0.030610265499794672 0.0 38-39 2.1183574740342334E-4 3.0262249629060474E-5 0.0 0.05385167321491312 0.0 40-41 2.420979970324838E-4 3.0262249629060474E-5 0.0 0.07772858817224182 0.0 42-43 2.420979970324838E-4 3.0262249629060474E-5 0.0 0.10369359835397572 0.0 44-45 2.420979970324838E-4 3.0262249629060474E-5 0.0 0.1362255167052157 0.0 46-47 2.420979970324838E-4 3.0262249629060474E-5 0.0 0.175899325968914 0.0 48-49 2.420979970324838E-4 3.0262249629060474E-5 0.0 0.22076311104399615 0.0 50-51 2.420979970324838E-4 3.0262249629060474E-5 0.0 0.2706806918071314 0.0 52-53 2.723602466615443E-4 3.0262249629060474E-5 0.0 0.32931380046343606 0.0 54-55 2.723602466615443E-4 3.0262249629060474E-5 0.0 0.39381778554777847 0.0 56-57 2.723602466615443E-4 3.0262249629060474E-5 0.0 0.47252989683296476 0.0 58-59 3.0262249629060475E-4 3.0262249629060474E-5 0.0 0.5610167147483376 0.0 60-61 3.0262249629060475E-4 3.0262249629060474E-5 0.0 0.6554954580902644 0.0 62-63 3.17753621105135E-4 3.0262249629060474E-5 0.0 0.7529399018958391 0.0 64-65 3.328847459196652E-4 3.0262249629060474E-5 0.0 0.8568150737475893 0.0 66-67 3.328847459196652E-4 6.052449925812095E-5 0.0 0.9770015981494029 0.0 68-69 3.631469955487257E-4 6.052449925812095E-5 0.0 1.1117745268724237 0.0 70-71 3.631469955487257E-4 6.052449925812095E-5 0.0 1.2600898123044493 0.0 72-73 3.631469955487257E-4 6.052449925812095E-5 0.0 1.4181798043666611 0.0 74-75 3.631469955487257E-4 6.052449925812095E-5 0.0 1.5806426915002723 0.0 76-77 3.631469955487257E-4 6.052449925812095E-5 0.0 1.7619438290279734 0.0 78-79 3.631469955487257E-4 6.052449925812095E-5 0.0 1.9657146869052522 0.0 80-81 3.631469955487257E-4 6.052449925812095E-5 0.0 2.183436441861528 0.0 82-83 3.631469955487257E-4 6.052449925812095E-5 0.0 2.4114019683172403 0.0 84-85 3.631469955487257E-4 6.052449925812095E-5 0.0 2.6429535713539964 0.0 86-87 3.631469955487257E-4 6.052449925812095E-5 0.0 2.893706571780392 0.0 88-89 3.631469955487257E-4 6.052449925812095E-5 0.0 3.169925255269641 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2325 0.0 57.421917 1 GTATCAA 4730 0.0 49.529556 1 ATCAACG 5515 0.0 40.30931 3 CAACGCA 5620 0.0 38.649136 5 TCAACGC 5640 0.0 38.430683 4 TATCAAC 6235 0.0 35.928444 2 AACGCAG 6350 0.0 34.124115 6 ACGCAGA 7090 0.0 29.969742 7 CGCAGAG 7105 0.0 29.048313 8 GTACATG 5320 0.0 25.575855 1 GCAGAGT 8090 0.0 23.920788 9 TACATGG 5505 0.0 23.226482 2 TCAACGA 335 0.0 22.51782 4 ACATGGG 5415 0.0 22.38449 3 AGAGTAC 7270 0.0 21.983284 10-11 GAGTACT 5330 0.0 20.215038 12-13 CAGAGTA 7860 0.0 19.808037 10-11 AGTACTT 6175 0.0 19.469563 12-13 GAGTACA 3935 0.0 18.328821 1 GTACTTT 6260 0.0 17.651123 14-15 >>END_MODULE