FastQCFastQC Report
Sat 18 Jun 2016
SRR1931031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304447
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68930.20859768669311388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54590.16520162072504113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35030.10600866045059884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41450.057.4528961
GTATCAA78250.042.5218771
ATCAACG96400.033.969433
CAACGCA97950.033.3681375
TCAACGC99400.032.9920044
TATCAAC101800.032.2166442
AACGCAG104250.031.7151936
ACGCAGA117200.027.6846567
CGCAGAG118050.027.28448
GCAGAGT140150.022.1693159
AGAGTAC125750.020.8791910-11
GAGTACT94000.020.84137212-13
GTACATG83950.020.0438561
CAGAGTA133850.019.95235310-11
TACATGG87050.019.2199762
ACGGGCG4250.018.97485
GGCGTCG4500.018.9745128
ACATGGG85000.018.7873863
GTACTTT105050.018.46848714-15
AGTACTT104350.018.20598412-13
Produced by FastQC (version 0.11.5)